Restriction map for
pFN21AE0646
(Circular) (Six-base) MAPSORT of: pFN21AE0646.seq Check: 5381 from: 1 to: 5845
pFN21AE0646 5845 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2509 2509
Size: 5845
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3188 1937
Size: 1251 4594
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2994 3908 5292 5560 1632
Size: 422 940 914 1384 268 1917
Fragments arranged by size:
1917 1384 940 914 422 268
AfeI AGC'GCT
Cuts at: 2075 2075
Size: 5845
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 4744 5721 1203
Size: 3541 977 1327
Fragments arranged by size:
3541 1327 977
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5845
AlwNI CAG_nnn'CTG
Cuts at: 2477 2845 3104 3588 5160 2477
Size: 368 259 484 1572 3162
Fragments arranged by size:
3162 1572 484 368 259
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 2678 3154 3380 3532 5641 1493
Size: 1185 476 226 152 2109 1697
Fragments arranged by size:
2109 1697 1185 476 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 2702 3170 1870
Size: 73 759 468 4545
Fragments arranged by size:
4545 759 468 73
BamHI G'GATC_C
Cuts at: 2899 3175 3542 2899
Size: 276 367 5202
Fragments arranged by size:
5202 367 276
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2981 3139 3166 4621 618
Size: 325 217 627 1194 158 27 1455 1842
Fragments arranged by size:
1842 1455 1194 627 325 217 158 27
BanII G_rGCy'C
Cuts at: 1391 2046 2327 2457 2672 3068 3164 1391
Size: 655 281 130 215 396 96 4072
Fragments arranged by size:
4072 655 396 281 215 130 96
BbeI G_GCGC'C
Cuts at: 2985 2985
Size: 5845
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2856 928
Size: 971 957 3917
Fragments arranged by size:
3917 971 957
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4036 4070 1903
Size: 34 50 34 2015 34 3678
Fragments arranged by size:
3678 2015 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4456 136
Size: 107 122 71 631 605 2784 1525
Fragments arranged by size:
2784 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5840 5840
Size: 5845
BlpI GC'TnA_GC
Cuts at: 2426 3262 2426
Size: 836 5009
Bme1580I G_kGCm'C
Cuts at: 1790 2539 2639 3142 3906 5062 1790
Size: 749 100 503 764 1156 2573
Fragments arranged by size:
2573 1156 764 749 503 100
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2503 3576 4534
Size: 416 53 83 399 24 1086 1073 958
Cuts at: 4534 442
Size: 1753
Fragments arranged by size:
1753 1086 1073 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 2328 2336 2807 2822 4505 1239
Size: 823 266 8 471 15 1683 2579
Fragments arranged by size:
2579 1683 823 471 266 15 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 882
Size: 617 3009 2219
Fragments arranged by size:
3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2640 3636 493
Size: 711 1436 996 2702
Fragments arranged by size:
2702 1436 996 711
BsaBI GATnn'nnATC
Cuts at: 2612 5839 2612
Size: 3227 2618
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2982 3652 4034 4669
Size: 53 83 186 936 1449 670 382 635
Cuts at: 4669 275
Size: 1451
Fragments arranged by size:
1451 1449 936 670 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2419 4278 4676 4950 5097 5508 2419
Size: 1859 398 274 147 411 2756
Fragments arranged by size:
2756 1859 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5620
Fragments arranged by size:
5620 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 2420 2720 2819 2981 3122 1186
Size: 1147 87 300 99 162 141 3909
Fragments arranged by size:
3909 1147 300 162 141 99 87
BseYI C'CCAG_C
Cuts at: 2111 2198 2218 2465 2561 2644 2729 5048 5544
Size: 87 20 247 96 83 85 2319 496
Cuts at: 5544 2111
Size: 2412
Fragments arranged by size:
2412 2319 496 247 96 87 85 83
20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2931 3295 4056 5084 1540
Size: 275 186 930 364 761 1028 2301
Fragments arranged by size:
2301 1028 930 761 364 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 2457 2811 3906 3991 5062 2427
Size: 30 354 1095 85 1071 3210
Fragments arranged by size:
3210 1095 1071 354 85 30
BsmI GAATG_Cn'
Cuts at: 3356 3449 3356
Size: 93 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2327 2427 2457 2539 2639 2672
Size: 399 256 281 100 30 82 100 33
Cuts at: 2672 2811 3068 3142 3164 3906 3991 5062 1391
Size: 139 257 74 22 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 281 257 256 139
100 100 85 82 74 33 30 22
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2509 3200 844
Size: 1665 691 3489
Fragments arranged by size:
3489 1665 691
BsrBI CCG'CTC
Cuts at: 1394 1823 3075 3732 1394
Size: 429 1252 657 3507
Fragments arranged by size:
3507 1252 657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4340 4514 65
Size: 1183 3092 174 1396
Fragments arranged by size:
3092 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4489 4676 1700
Size: 110 2679 187 2869
Fragments arranged by size:
2869 2679 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 2538 3905 4917 2538
Size: 1367 1012 3466
Fragments arranged by size:
3466 1367 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2782 794
Size: 1988 3857
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 2899 3175 3542 3927 3944 5396 5482 5494
Size: 927 276 367 385 17 1452 86 12
Cuts at: 5494 5840 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 927 385 367 346 276 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2121 2712 513
Size: 552 1056 591 3646
Fragments arranged by size:
3646 1056 591 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3096 487
Size: 683 56 1870 3236
Fragments arranged by size:
3236 1870 683 56
BtsI GCAGTG_nn'
Cuts at: 2244 2455 2968 3361 4155 4175 2244
Size: 211 513 393 794 20 3914
Fragments arranged by size:
3914 794 513 393 211 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DraIII CAC_nnn'GTG
Cuts at: 3016 3016
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2177 4852 809
Size: 507 861 2675 1802
Fragments arranged by size:
2675 1802 861 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2530 2928 3132
Size: 54 1425 50 275 18 700 398 204
Cuts at: 3132 3292 4181 5810 8
Size: 160 889 1629 43
Fragments arranged by size:
1629 1425 889 700 398 275 204 160
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2928 3292 1537
Size: 275 1116 364 4090
Fragments arranged by size:
4090 1116 364 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 2292 2970 3775 1353
Size: 845 94 678 805 3423
Fragments arranged by size:
3423 845 805 678 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2455 2455
Size: 5845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2062 2328 2336 2807 2822 2994
Size: 393 422 8 266 8 471 15 172
Cuts at: 2994 3908 4505 5292 5560 1239
Size: 914 597 787 268 1524
Fragments arranged by size:
1524 914 787 597 471 422 393 268
266 172 15 8 8
EcoO109I rG'GnC_Cy
Cuts at: 2287 2519 2287
Size: 232 5613
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 2077 2387 2985 4992 1678
Size: 399 310 598 2007 2531
Fragments arranged by size:
2531 2007 598 399 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2592 2596 2624 2798
Size: 32 135 32 992 28 4 28 174
Cuts at: 2798 2830 4486 4518 4560 4592 1373
Size: 32 1656 32 42 32 2626
Fragments arranged by size:
2626 1656 992 174 135 42 32 32
32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3189 3435 669
Size: 988 281 1251 246 3079
Fragments arranged by size:
3079 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KasI G'GCGC_C
Cuts at: 2981 2981
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3000 4959 5143 1305
Size: 1695 1959 184 2007
Fragments arranged by size:
2007 1959 1695 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4023
Fragments arranged by size:
4023 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 2880 3803 4162 4321 518
Size: 648 1521 193 923 359 159 2042
Fragments arranged by size:
2042 1521 923 648 359 193 159
MspA1I CmG'CkG
Cuts at: 1606 3115 3261 3938 5086 5331 1606
Size: 1509 146 677 1148 245 2120
Fragments arranged by size:
2120 1509 1148 677 245 146
NarI GG'CG_CC
Cuts at: 2982 2982
Size: 5845
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5293
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 2723 3203 4748 1132
Size: 97 1494 480 1545 2229
Fragments arranged by size:
2229 1545 1494 480 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5845
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2477 2845 1108
Size: 1369 368 4108
Fragments arranged by size:
4108 1369 368
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PmlI CAC'GTG
Cuts at: 2640 2640
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PstI C_TGCA'G
Cuts at: 830 2462 2595 3197 830
Size: 1632 133 602 3478
Fragments arranged by size:
3478 1632 602 133
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 3115 3115
Size: 5845
SacI G_AGCT'C
Cuts at: 2457 2457
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SfcI C'TryA_G
Cuts at: 826 1045 2350 2458 2591 3193 4328 5009 5200
Size: 219 1305 108 133 602 1135 681 191
Cuts at: 5200 826
Size: 1471
Fragments arranged by size:
1471 1305 1135 681 602 219 191 133
108
SfoI GGC'GCC
Cuts at: 2983 2983
Size: 5845
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3949 4850 5112 820
Size: 197 926 2006 901 262 1553
Fragments arranged by size:
2006 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 2723 3203 1132
Size: 1591 480 3774
Fragments arranged by size:
3774 1591 480
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 3123 1517
Size: 1606 4239
StyI C'CwwG_G
Cuts at: 513 1065 3027 3284 513
Size: 552 1962 257 3074
Fragments arranged by size:
3074 1962 552 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3872 3889 4042 4227 4672 325
Size: 1255 2292 17 153 185 445 1498
Fragments arranged by size:
2292 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 4091 5803
Size: 274 80 33 51 1443 534 1580 1712
Cuts at: 5803 96
Size: 138
Fragments arranged by size:
1712 1580 1443 534 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5805 5837 5805
Size: 32 5813
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2119
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI