Restriction map for
pFN21AE0645
(Circular) (Six-base) MAPSORT of: pFN21AE0645.seq Check: 2620 from: 1 to: 5356
pFN21AE0645 5356 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3166 278
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2400 2699 1937
Size: 463 299 4594
Fragments arranged by size:
4594 463 299
Acc65I G'GTAC_C
Cuts at: 2677 2677
Size: 5356
AclI AA'CG_TT
Cuts at: 1563 3483 3856 1563
Size: 1920 373 3063
Fragments arranged by size:
3063 1920 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3419 4803 5071 1632
Size: 1787 1384 268 1917
Fragments arranged by size:
1917 1787 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5159
AflIII A'CryG_T
Cuts at: 1203 4255 5232 1203
Size: 3052 977 1327
Fragments arranged by size:
3052 1327 977
AgeI A'CCGG_T
Cuts at: 4187 4187
Size: 5356
AhdI GACnn_n'nnGTC
Cuts at: 4085 4085
Size: 5356
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5356
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2360 2392 2360
Size: 32 5324
AlwNI CAG_nnn'CTG
Cuts at: 3099 4671 3099
Size: 1572 3784
ApaLI G'TGCA_C
Cuts at: 2648 3413 4569 2648
Size: 765 1156 3435
Fragments arranged by size:
3435 1156 765
ApoI r'AATT_y
Cuts at: 1493 2665 2891 3043 5152 1493
Size: 1172 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3910 160
Size: 872 2878 1606
Fragments arranged by size:
2878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
AvaI C'yCGr_G
Cuts at: 1870 1943 2188 2347 2681 1870
Size: 73 245 159 334 4545
Fragments arranged by size:
4545 334 245 159 73
BamHI G'GATC_C
Cuts at: 2686 3053 2686
Size: 367 4989
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2677 4132 618
Size: 325 217 627 890 1455 1842
Fragments arranged by size:
1842 1455 890 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2127 2276 2463 2675 1391
Size: 736 149 187 212 4072
Fragments arranged by size:
4072 736 212 187 149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3547 3581 1903
Size: 34 1610 34 3678
Fragments arranged by size:
3678 1610 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3248 4458 3248
Size: 1210 4146
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3967 136
Size: 107 122 71 631 605 2295 1525
Fragments arranged by size:
2295 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2439 5351 2439
Size: 2912 2444
BlpI GC'TnA_GC
Cuts at: 2534 2773 2534
Size: 239 5117
Bme1580I G_kGCm'C
Cuts at: 1790 2652 3417 4573 1790
Size: 862 765 1156 2573
Fragments arranged by size:
2573 1156 862 765
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2494 3087 4045
Size: 416 53 83 399 24 1077 593 958
Cuts at: 4045 442
Size: 1753
Fragments arranged by size:
1753 1077 958 593 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5356
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2093
Size: 5356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2588 4016 1239
Size: 1349 1428 2579
Fragments arranged by size:
2579 1428 1349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2453 3481 4346 4644 2063
Size: 390 1028 865 298 2775
Fragments arranged by size:
2775 1028 865 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2304 4019 882
Size: 617 805 1715 2219
Fragments arranged by size:
2219 1715 805 617
BsaAI yAC'GTr
Cuts at: 493 1204 2638 3147 493
Size: 711 1434 509 2702
Fragments arranged by size:
2702 1434 711 509
BsaBI GATnn'nnATC
Cuts at: 5350 5350
Size: 5356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3163 3545 4180 275
Size: 53 83 186 936 1630 382 635 1451
Fragments arranged by size:
1630 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3789 4187 4461 4608 5019 3789
Size: 398 274 147 411 4126
Fragments arranged by size:
4126 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2301 2331 2360 2390 1178
Size: 30 165 30 898 30 29 30 4144
Fragments arranged by size:
4144 898 165 30 30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 2471 1186
Size: 1198 87 4071
Fragments arranged by size:
4071 1198 87
BseYI C'CCAG_C
Cuts at: 4559 5055 4559
Size: 496 4860
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 2660 1163
Size: 573 603 321 3859
Fragments arranged by size:
3859 603 573 321
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2806 3567 4595 1540
Size: 275 186 805 761 1028 2301
Fragments arranged by size:
2301 1028 805 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2276 2652 3417 3502 4573 2127
Size: 149 376 765 85 1071 2910
Fragments arranged by size:
2910 1071 765 376 149 85
BsmI GAATG_Cn'
Cuts at: 2867 2960 2867
Size: 93 5263
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2276 2463 2652 2675 3417 3502
Size: 399 337 149 187 189 23 742 85
Cuts at: 3502 4573 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 337 189 187 149
85 23
BspEI T'CCGG_A
Cuts at: 5019 5019
Size: 5356
BspHI T'CATG_A
Cuts at: 3245 4975 3245
Size: 1730 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2711 844
Size: 1867 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3243 1394
Size: 429 1420 3507
Fragments arranged by size:
3507 1420 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3851 4025 65
Size: 1183 2603 174 1396
Fragments arranged by size:
2603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2598 4000 4187 1700
Size: 110 788 1402 187 2869
Fragments arranged by size:
2869 1402 788 187 110
BsrGI T'GTAC_A
Cuts at: 96 2022 96
Size: 1926 3430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5356
BssSI C'ACGA_G
Cuts at: 3416 4428 3416
Size: 1012 4344
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2647 794
Size: 1853 3503
BstBI TT'CG_AA
Cuts at: 4160 4160
Size: 5356
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5356
BstYI r'GATC_y
Cuts at: 1972 2082 2439 2686 3053 3438 3455 4907 4993
Size: 110 357 247 367 385 17 1452 86
Cuts at: 4993 5005 5351 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 357 346 247 110
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2180 2545 2627 513
Size: 552 939 9 167 365 82 3242
Fragments arranged by size:
3242 939 552 365 167 82 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2407 487
Size: 683 56 1181 3436
Fragments arranged by size:
3436 1181 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 3666 3686 2872
Size: 794 20 4542
Fragments arranged by size:
4542 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5321
DraI TTT'AAA
Cuts at: 2660 2660
Size: 5356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4363 809
Size: 507 3047 1802
Fragments arranged by size:
3047 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2803 3692
Size: 54 1425 50 275 18 381 592 889
Cuts at: 3692 5321 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 592 381 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2803 1537
Size: 275 991 4090
Fragments arranged by size:
4090 991 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 3286 1353
Size: 858 1075 3423
Fragments arranged by size:
3423 1075 858
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2531 3943 4317 4463 387
Size: 1064 138 942 1412 374 146 1280
Fragments arranged by size:
1412 1280 1064 942 374 146 138
EcoICRI GAG'CTC
Cuts at: 2125 2274 2125
Size: 149 5207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2588 3419 4016 4803 5071 1239
Size: 393 956 831 597 787 268 1524
Fragments arranged by size:
1524 956 831 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2366
Size: 5356
EcoO109I rG'GnC_Cy
Cuts at: 2481 2521 2563 2481
Size: 40 42 5274
Fragments arranged by size:
5274 42 40
EcoRI G'AATT_C
Cuts at: 2665 3043 2665
Size: 378 4978
FspI TGC'GCA
Cuts at: 3862 3862
Size: 5356
HaeII r_GCGC'y
Cuts at: 1678 2575 4503 1678
Size: 897 1928 2531
Fragments arranged by size:
2531 1928 897
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373
Size: 32 135 32 2425 32 42 32 2626
Fragments arranged by size:
2626 2425 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2401 2700 2946 669
Size: 988 281 463 299 246 3079
Fragments arranged by size:
3079 988 463 299 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5356
HpaI GTT'AAC
Cuts at: 2946 2946
Size: 5356
KpnI G_GTAC'C
Cuts at: 2681 2681
Size: 5356
MfeI C'AATT_G
Cuts at: 2955 2955
Size: 5356
MluI A'CGCG_T
Cuts at: 5232 5232
Size: 5356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4470 4654 1305
Size: 3165 184 2007
Fragments arranged by size:
3165 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3534
Fragments arranged by size:
3534 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3314 3673 3832 518
Size: 648 2148 359 159 2042
Fragments arranged by size:
2148 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2209 2772 3449 4597 4842 1606
Size: 603 563 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 603 563 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3865
Fragments arranged by size:
3865 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5356
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5356
NotI GC'GGCC_GC
Cuts at: 2803 2803
Size: 5356
NspI r_CATG'y
Cuts at: 1132 1229 2714 4259 1132
Size: 97 1485 1545 2229
Fragments arranged by size:
2229 1545 1485 97
PasI CC'CwG_GG
Cuts at: 1348 2279 1348
Size: 931 4425
PciI A'CATG_T
Cuts at: 4255 4255
Size: 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5356
PfoI T'CCnGG_A
Cuts at: 2259 2565 2259
Size: 306 5050
PmeI GTTT'AAAC
Cuts at: 2660 2660
Size: 5356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2360 2392 3425 3457 4938 4970 2360
Size: 32 1033 32 1481 32 2746
Fragments arranged by size:
2746 1481 1033 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2521 2563 2521
Size: 42 5314
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5356
PsiI TTA'TAA
Cuts at: 2926 2926
Size: 5356
PstI C_TGCA'G
Cuts at: 830 2708 830
Size: 1878 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5356
PvuII CAG'CTG
Cuts at: 2209 2209
Size: 5356
SacI G_AGCT'C
Cuts at: 2127 2276 2127
Size: 149 5207
SalI G'TCGA_C
Cuts at: 1936 2399 2698 1936
Size: 463 299 4594
Fragments arranged by size:
4594 463 299
SbfI CC_TGCA'GG
Cuts at: 2708 2708
Size: 5356
ScaI AGT'ACT
Cuts at: 2357 3604 5316 2357
Size: 1247 1712 2397
Fragments arranged by size:
2397 1712 1247
SexAI A'CCwGG_T
Cuts at: 2313 2652 2313
Size: 339 5017
SfcI C'TryA_G
Cuts at: 826 1045 2037 2704 3839 4520 4711 826
Size: 219 992 667 1135 681 191 1471
Fragments arranged by size:
1471 1135 992 681 667 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5356
SmaI CCC'GGG
Cuts at: 1872 2683 1872
Size: 811 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2468 3460 4361 4623 820
Size: 197 926 135 390 992 901 262 1553
Fragments arranged by size:
1553 992 926 901 390 262 197 135
SnaBI TAC'GTA
Cuts at: 493 3147 493
Size: 2654 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5356
SphI G_CATG'C
Cuts at: 1132 2714 1132
Size: 1582 3774
SspI AAT'ATT
Cuts at: 5 52 3280 5
Size: 47 3228 2081
Fragments arranged by size:
3228 2081 47
StuI AGG'CCT
Cuts at: 1517 2345 1517
Size: 828 4528
StyI C'CwwG_G
Cuts at: 513 1065 2004 2795 513
Size: 552 939 791 3074
Fragments arranged by size:
3074 939 791 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3383 3400 3553 3738 4183 325
Size: 1255 1803 17 153 185 445 1498
Fragments arranged by size:
1803 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 2355 3602
Size: 274 80 33 51 1443 45 333 1247
Cuts at: 3602 5314 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1247 333 274 138 80 51
45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 2301 2333 5316 5348 2067
Size: 32 202 32 2983 32 2075
Fragments arranged by size:
2983 2075 202 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5356
XbaI T'CTAG_A
Cuts at: 2692 2692
Size: 5356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2583 2011
Size: 572 4784
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5356
XmaI C'CCGG_G
Cuts at: 1870 2681 1870
Size: 811 4545
XmnI GAAnn'nnTTC
Cuts at: 2220 2357 3485 2220
Size: 137 1128 4091
Fragments arranged by size:
4091 1128 137
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3164 276
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI