Restriction map for
pFN21AE0636
(Circular) (Six-base) MAPSORT of: pFN21AE0636.seq Check: 2758 from: 1 to: 5839
pFN21AE0636 5839 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2827 3902 5286 5554 1632
Size: 1195 1075 1384 268 1917
Fragments arranged by size:
1917 1384 1195 1075 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 2785 4738 5715 1203
Size: 1582 1953 977 1327
Fragments arranged by size:
1953 1582 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 2939 1166
Size: 1773 4066
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 2246 3582 5154 2246
Size: 1336 1572 2931
Fragments arranged by size:
2931 1572 1336
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 2331 2625 2961 3129 3148 3374 3526 5635
Size: 838 294 336 168 19 226 152 2109
Cuts at: 5635 1493
Size: 1697
Fragments arranged by size:
2109 1697 838 336 294 226 168 152
19
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 1870
Size: 73 1221 4545
Fragments arranged by size:
4545 1221 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2890 2923 2890
Size: 33 5806
BamHI G'GATC_C
Cuts at: 3169 3536 3169
Size: 367 5472
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 4615 618
Size: 325 217 627 1373 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2237 3158 1391
Size: 846 921 4072
Fragments arranged by size:
4072 921 846
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4868
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3731 4941 3731
Size: 1210 4629
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 5380
BfrBI ATG'CAT
Cuts at: 2680 2680
Size: 5839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4450 136
Size: 107 122 71 631 605 2778 1525
Fragments arranged by size:
2778 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 3900 5056 1790
Size: 2110 1156 2573
Fragments arranged by size:
2573 2110 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4528 442
Size: 416 53 83 399 24 2153 958 1753
Fragments arranged by size:
2153 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2223 2739 4499 1239
Size: 984 516 1760 2579
Fragments arranged by size:
2579 1760 984 516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2162 2825 3964 4829 5127 2162
Size: 663 1139 865 298 2874
Fragments arranged by size:
2874 1139 865 663 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2635 4502 882
Size: 617 1136 1867 2219
Fragments arranged by size:
2219 1867 1136 617
BsaAI yAC'GTr
Cuts at: 493 1204 2788 3630 493
Size: 711 1584 842 2702
Fragments arranged by size:
2702 1584 842 711
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3646 4028 4663 275
Size: 53 83 186 936 2113 382 635 1451
Fragments arranged by size:
2113 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5614
Fragments arranged by size:
5614 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2761 3020 1186
Size: 892 683 259 4005
Fragments arranged by size:
4005 892 683 259
BseYI C'CCAG_C
Cuts at: 5042 5538 5042
Size: 496 5343
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2758 1163
Size: 573 1022 4244
Fragments arranged by size:
4244 1022 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 2372 3900 3985 5056 2237
Size: 135 1528 85 1071 3020
Fragments arranged by size:
3020 1528 1071 135 85
BsmI GAATG_Cn'
Cuts at: 2489 2498 2573 3350 3443 2489
Size: 9 75 777 93 4885
Fragments arranged by size:
4885 777 93 75 9
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2372 3158 3900 3985 5056 1391
Size: 399 447 135 786 742 85 1071 2174
Fragments arranged by size:
2174 1071 786 742 447 399 135 85
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3194 844
Size: 2350 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3726 1394
Size: 429 1903 3507
Fragments arranged by size:
3507 1903 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2578 2662 4334 4508 65
Size: 1183 1330 84 1672 174 1396
Fragments arranged by size:
1672 1396 1330 1183 174 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 3118 96
Size: 3022 2817
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 2228 2693 3899 4911 2228
Size: 465 1206 1012 3156
Fragments arranged by size:
3156 1206 1012 465
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2493 2609 794
Size: 1699 116 4024
Fragments arranged by size:
4024 1699 116
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5839
BstYI r'GATC_y
Cuts at: 1972 3169 3536 3921 3938 5390 5476 5488 5834
Size: 1197 367 385 17 1452 86 12 346
Cuts at: 5834 1972
Size: 1977
Fragments arranged by size:
1977 1452 1197 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 4151
Fragments arranged by size:
4151 1136 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5100
Fragments arranged by size:
5100 683 56
BtsI GCAGTG_nn'
Cuts at: 2055 2494 3355 4149 4169 2055
Size: 439 861 794 20 3725
Fragments arranged by size:
3725 861 794 439 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2560 2595 2644 2679 529
Size: 35 1996 35 49 35 3689
Fragments arranged by size:
3689 1996 49 35 35 35
DraI TTT'AAA
Cuts at: 3143 3143
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3286 4175 5804
Size: 54 1425 50 275 18 1456 889 1629
Cuts at: 5804 8
Size: 43
Fragments arranged by size:
1629 1456 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3769 1353
Size: 2416 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2235 2235
Size: 5839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2739 2827 3902 4499 5286 5554
Size: 393 591 516 88 1075 597 787 268
Cuts at: 5554 1239
Size: 1524
Fragments arranged by size:
1524 1075 787 597 591 516 393 268
88
EcoNI CCTnn'n_nnAGG
Cuts at: 2593 2593
Size: 5839
EcoRI G'AATT_C
Cuts at: 2961 3148 3526 2961
Size: 187 378 5274
Fragments arranged by size:
5274 378 187
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 4986 1678
Size: 3308 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2359 2391 4480 4512 4554
Size: 32 135 32 787 32 2089 32 42
Cuts at: 4554 4586 1373
Size: 32 2626
Fragments arranged by size:
2626 2089 787 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3429 669
Size: 988 281 1245 246 3079
Fragments arranged by size:
3079 1245 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2922 748
Size: 2174 3665
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 4953 5137 1305
Size: 792 2856 184 2007
Fragments arranged by size:
2856 2007 792 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4017
Fragments arranged by size:
4017 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2818 2939 3797 4156 4315 518
Size: 648 975 677 121 858 359 159 2042
Fragments arranged by size:
2042 975 858 677 648 359 159 121
MspA1I CmG'CkG
Cuts at: 1606 2074 3255 3932 5080 5325 1606
Size: 468 1181 677 1148 245 2120
Fragments arranged by size:
2120 1181 1148 677 468 245
NcoI C'CATG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 4151
Fragments arranged by size:
4151 1136 552
NdeI CA'TA_TG
Cuts at: 387 2912 387
Size: 2525 3314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NsiI A_TGCA'T
Cuts at: 2682 2682
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 3197 4742 1132
Size: 97 1968 1545 2229
Fragments arranged by size:
2229 1968 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2041 2695 1108
Size: 933 654 4252
Fragments arranged by size:
4252 933 654
PfoI T'CCnGG_A
Cuts at: 2016 2034 2016
Size: 18 5821
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PmlI CAC'GTG
Cuts at: 2788 2788
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 2361 2865 3409 2361
Size: 504 544 4791
Fragments arranged by size:
4791 544 504
PstI C_TGCA'G
Cuts at: 830 2062 3191 830
Size: 1232 1129 3478
Fragments arranged by size:
3478 1232 1129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 2074 2074
Size: 5839
SacI G_AGCT'C
Cuts at: 2237 2237
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SfcI C'TryA_G
Cuts at: 826 1045 2058 2125 2466 2590 3187 4322 5003
Size: 219 1013 67 341 124 597 1135 681
Cuts at: 5003 5194 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1013 681 597 341 219 191
124 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2177 2804 3943 4844 5106 820
Size: 197 926 234 627 1139 901 262 1553
Fragments arranged by size:
1553 1139 926 901 627 262 234 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5839
StyI C'CwwG_G
Cuts at: 513 1065 2201 3278 513
Size: 552 1136 1077 3074
Fragments arranged by size:
3074 1136 1077 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3866 3883 4036 4221 4666 325
Size: 1255 2286 17 153 185 445 1498
Fragments arranged by size:
2286 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3118 4085 5797
Size: 274 80 33 51 1443 1141 967 1712
Cuts at: 5797 96
Size: 138
Fragments arranged by size:
1712 1443 1141 967 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5799 5831 5799
Size: 32 5807
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 2504 2756 2840 3968 2504
Size: 252 84 1128 4375
Fragments arranged by size:
4375 1128 252 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI