Restriction map for
pFN21AE0633
(Circular) (Six-base) MAPSORT of: pFN21AE0633.seq Check: 3570 from: 1 to: 5311
pFN21AE0633 5311 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 1937
Size: 717 4594
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3374 4758 5026 1632
Size: 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 4210 5187 1203
Size: 3007 977 1327
Fragments arranged by size:
3007 1327 977
AgeI A'CCGG_T
Cuts at: 2097 4142 2097
Size: 2045 3266
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2359 1184
Size: 1175 4136
AlwNI CAG_nnn'CTG
Cuts at: 3054 4626 3054
Size: 1572 3739
ApaLI G'TGCA_C
Cuts at: 2306 3368 4524 2306
Size: 1062 1156 3093
Fragments arranged by size:
3093 1156 1062
ApoI r'AATT_y
Cuts at: 1493 2620 2846 2998 5107 1493
Size: 1127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2321 2415 2441 2596 2636 1870
Size: 73 378 94 26 155 40 4545
Fragments arranged by size:
4545 378 155 94 73 40 26
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 4087 618
Size: 325 217 627 845 1455 1842
Fragments arranged by size:
1842 1455 845 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2603 2630 1391
Size: 1212 27 4072
Fragments arranged by size:
4072 1212 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3502 3536 1903
Size: 34 1565 34 3678
Fragments arranged by size:
3678 1565 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 2469 1548
Size: 921 4390
BfrBI ATG'CAT
Cuts at: 2346 2346
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3922 136
Size: 107 122 71 631 605 2250 1525
Fragments arranged by size:
2250 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5306 5306
Size: 5311
BlpI GC'TnA_GC
Cuts at: 2728 2728
Size: 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2310 3372 4528 1790
Size: 520 1062 1156 2573
Fragments arranged by size:
2573 1156 1062 520
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5311
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3042 4000 442
Size: 416 53 83 399 24 1625 958 1753
Fragments arranged by size:
1753 1625 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2356 3971 1239
Size: 1117 1615 2579
Fragments arranged by size:
2579 1615 1117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4301 4599 3436
Size: 865 298 4148
Fragments arranged by size:
4148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3974 882
Size: 617 2475 2219
Fragments arranged by size:
2475 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 493
Size: 711 1898 2702
Fragments arranged by size:
2702 1898 711
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3118 3500 4135 275
Size: 53 83 186 936 1585 382 635 1451
Fragments arranged by size:
1585 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2097 2238 3744 4142 4416 4563 4974 2097
Size: 141 1506 398 274 147 411 2434
Fragments arranged by size:
2434 1506 411 398 274 147 141
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 1178
Size: 30 165 30 684 30 4372
Fragments arranged by size:
4372 684 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2542 1186
Size: 1075 281 3955
Fragments arranged by size:
3955 1075 281
BseYI C'CCAG_C
Cuts at: 4514 5010 4514
Size: 496 4815
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4738
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2761 3522 4550 1540
Size: 275 186 760 761 1028 2301
Fragments arranged by size:
2301 1028 761 760 275 186
BsiHKAI G_wGCw'C
Cuts at: 2105 2310 3372 3457 4528 2105
Size: 205 1062 85 1071 2888
Fragments arranged by size:
2888 1071 1062 205 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2310 2603 2630 3372 3457 4528
Size: 399 315 205 293 27 742 85 1071
Cuts at: 4528 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 315 293 205 85
27
BspEI T'CCGG_A
Cuts at: 2238 4974 2238
Size: 2736 2575
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2396 2666 844
Size: 1552 270 3489
Fragments arranged by size:
3489 1552 270
BsrBI CCG'CTC
Cuts at: 1394 1823 2260 3198 1394
Size: 429 437 938 3507
Fragments arranged by size:
3507 938 437 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2097 3955 4142 1700
Size: 110 275 12 1858 187 2869
Fragments arranged by size:
2869 1858 275 187 110 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5311
BssSI C'ACGA_G
Cuts at: 2213 3371 4383 2213
Size: 1158 1012 3141
Fragments arranged by size:
3141 1158 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2305 794
Size: 1511 3800
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 2408 1184
Size: 1224 4087
BstYI r'GATC_y
Cuts at: 1972 2641 3008 3393 3410 4862 4948 4960 5306
Size: 669 367 385 17 1452 86 12 346
Cuts at: 5306 1972
Size: 1977
Fragments arranged by size:
1977 1452 669 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2265 513
Size: 552 939 261 3559
Fragments arranged by size:
3559 939 552 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3621 3641 2827
Size: 794 20 4497
Fragments arranged by size:
4497 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DraIII CAC_nnn'GTG
Cuts at: 2215 2215
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2430 2481 2758
Size: 54 1425 50 275 18 600 51 277
Cuts at: 2758 3647 5276 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 600 277 275 54 51
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2758 1537
Size: 275 946 4090
Fragments arranged by size:
4090 946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 3241 1353
Size: 819 1069 3423
Fragments arranged by size:
3423 1069 819
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3898 4272 4418 387
Size: 1064 138 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2356 3374 3971 4758 5026 1239
Size: 393 724 1018 597 787 268 1524
Fragments arranged by size:
1524 1018 787 724 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2395 2494 2545 2395
Size: 99 51 5161
Fragments arranged by size:
5161 99 51
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 4458 1678
Size: 2780 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2439 2471 2537
Size: 32 135 32 515 32 320 32 66
Cuts at: 2537 2569 3952 3984 4026 4058 1373
Size: 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 515 320 135 66 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 2655 2901 669
Size: 988 281 115 602 246 3079
Fragments arranged by size:
3079 988 602 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2053 2901 2053
Size: 848 4463
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4425 4609 1305
Size: 3120 184 2007
Fragments arranged by size:
3120 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2432 10
Size: 54 1425 343 600 2889
Fragments arranged by size:
2889 1425 600 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 2477 3269 3628 3787 518
Size: 648 1147 164 792 359 159 2042
Fragments arranged by size:
2042 1147 792 648 359 164 159
MspA1I CmG'CkG
Cuts at: 1606 2286 2727 3404 4552 4797 1606
Size: 680 441 677 1148 245 2120
Fragments arranged by size:
2120 1148 680 677 441 245
NaeI GCC'GGC
Cuts at: 2087 2087
Size: 5311
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3820
Fragments arranged by size:
3820 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NgoMIV G'CCGG_C
Cuts at: 2085 2085
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2758 2758
Size: 5311
NsiI A_TGCA'T
Cuts at: 2348 2348
Size: 5311
NspI r_CATG'y
Cuts at: 1132 1229 2669 4214 1132
Size: 97 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5311
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2215 1108
Size: 1107 4204
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2395 2545 2395
Size: 150 5161
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2157 2663 830
Size: 1327 506 3478
Fragments arranged by size:
3478 1327 506
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SanDI GG'GwC_CC
Cuts at: 2395 2395
Size: 5311
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2153 2659 3794 4475 4666 826
Size: 219 1108 506 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 681 506 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2598 2638 1872
Size: 726 40 4545
Fragments arranged by size:
4545 726 40
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3415 4316 4578 820
Size: 197 926 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 2015 3235 5
Size: 47 1963 1220 2081
Fragments arranged by size:
2081 1963 1220 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5311
StyI C'CwwG_G
Cuts at: 513 1065 2004 2200 2750 513
Size: 552 939 196 550 3074
Fragments arranged by size:
3074 939 552 550 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3338 3355 3508 3693 4138 325
Size: 1255 1758 17 153 185 445 1498
Fragments arranged by size:
1758 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2596 2636 1870
Size: 726 40 4545
Fragments arranged by size:
4545 726 40
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI