Restriction map for
pFN21AE0630
(Circular) (Six-base) MAPSORT of: pFN21AE0630.seq Check: 4905 from: 1 to: 5818
pFN21AE0630 5818 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3161 1937
Size: 1224 4594
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 2072 3945 4318 1563
Size: 509 1873 373 3063
Fragments arranged by size:
3063 1873 509 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3881 5265 5533 1632
Size: 2249 1384 268 1917
Fragments arranged by size:
2249 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 2245 4717 5694 1203
Size: 1042 2472 977 1327
Fragments arranged by size:
2472 1327 1042 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 4547 4547
Size: 5818
AleI CACnn'nnGTG
Cuts at: 1166 2114 1166
Size: 948 4870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5818
AlwNI CAG_nnn'CTG
Cuts at: 2491 3561 5133 2491
Size: 1070 1572 3176
Fragments arranged by size:
3176 1572 1070
ApaI G_GGCC'C
Cuts at: 3043 3043
Size: 5818
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 2135 3127 3353 3505 5614 1493
Size: 642 992 226 152 2109 1697
Fragments arranged by size:
2109 1697 992 642 226 152
AscI GG'CGCG_CC
Cuts at: 2273 2273
Size: 5818
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2662 2934 3075 3143 1870
Size: 73 719 272 141 68 4545
Fragments arranged by size:
4545 719 272 141 73 68
AvrII C'CTAG_G
Cuts at: 2692 2692
Size: 5818
BamHI G'GATC_C
Cuts at: 3148 3515 3148
Size: 367 5451
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2580 3002 3051 3060 3139
Size: 325 217 627 793 422 49 9 79
Cuts at: 3139 4594 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 793 627 422 325 217 79
49 9
BanII G_rGCy'C
Cuts at: 1391 2342 2388 3043 3137 1391
Size: 951 46 655 94 4072
Fragments arranged by size:
4072 951 655 94 46
BbeI G_GCGC'C
Cuts at: 3006 3055 3064 3006
Size: 49 9 5760
Fragments arranged by size:
5760 49 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2159 2619 928
Size: 971 260 460 4127
Fragments arranged by size:
4127 971 460 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4009 4043 1903
Size: 34 2072 34 3678
Fragments arranged by size:
3678 2072 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2872 4429 136
Size: 107 122 71 631 605 1200 1557 1525
Fragments arranged by size:
1557 1525 1200 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2198 5813 2198
Size: 3615 2203
BlpI GC'TnA_GC
Cuts at: 3235 3235
Size: 5818
Bme1580I G_kGCm'C
Cuts at: 1790 2766 2985 3043 3879 5035 1790
Size: 976 219 58 836 1156 2573
Fragments arranged by size:
2573 1156 976 836 219 58
BmgBI CAC'GTC
Cuts at: 1318 2569 1318
Size: 1251 4567
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2018 2704 3549
Size: 416 53 83 399 24 601 686 845
Cuts at: 3549 4507 442
Size: 958 1753
Fragments arranged by size:
1753 958 845 686 601 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 2498 2669 3065 4478 1239
Size: 1163 96 171 396 1413 2579
Fragments arranged by size:
2579 1413 1163 396 171 96
Bpu10I CC'TnA_GC
Cuts at: 2888 2888
Size: 5818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 2144 3943 4808 5106 2132
Size: 12 1799 865 298 2844
Fragments arranged by size:
2844 1799 865 298 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2159 4481 882
Size: 617 660 2322 2219
Fragments arranged by size:
2322 2219 660 617
BsaAI yAC'GTr
Cuts at: 493 1204 3609 493
Size: 711 2405 2702
Fragments arranged by size:
2702 2405 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3003 3052 3061 3625
Size: 53 83 186 936 1470 49 9 564
Cuts at: 3625 4007 4642 275
Size: 382 635 1451
Fragments arranged by size:
1470 1451 936 635 564 382 186 83
53 49 9
BsaWI w'CCGG_w
Cuts at: 4251 4649 4923 5070 5481 4251
Size: 398 274 147 411 4588
Fragments arranged by size:
4588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2689 2719 2953 2983 1178
Size: 30 165 30 1286 30 234 30 4013
Fragments arranged by size:
4013 1286 234 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2666 2925 1186
Size: 1258 222 259 4079
Fragments arranged by size:
4079 1258 259 222
BseYI C'CCAG_C
Cuts at: 2875 5021 5517 2875
Size: 2146 496 3176
Fragments arranged by size:
3176 2146 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 3007 1163
Size: 573 336 935 3974
Fragments arranged by size:
3974 935 573 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 2867 3268 4029 5057 1540
Size: 275 186 832 34 401 761 1028 2301
Fragments arranged by size:
2301 1028 832 761 401 275 186 34
BsiHKAI G_wGCw'C
Cuts at: 3879 3964 5035 3879
Size: 85 1071 4662
Fragments arranged by size:
4662 1071 85
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
BsmBI CGTCTCn'nnnn_
Cuts at: 2907 2907
Size: 5818
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2342 2388 2766 2985 3043 3137 3879
Size: 399 552 46 378 219 58 94 742
Cuts at: 3879 3964 5035 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 399 378 219 94
85 58 46
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2811 3106 3173 844
Size: 1967 295 67 3489
Fragments arranged by size:
3489 1967 295 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2892 3049 3705 1394
Size: 429 1069 157 656 3507
Fragments arranged by size:
3507 1069 656 429 157
BsrDI GCAATG_nn'
Cuts at: 65 1248 4313 4487 65
Size: 1183 3065 174 1396
Fragments arranged by size:
3065 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2616 4462 4649 1700
Size: 110 806 1846 187 2869
Fragments arranged by size:
2869 1846 806 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 2273 2774 1925
Size: 348 501 4969
Fragments arranged by size:
4969 501 348
BssSI C'ACGA_G
Cuts at: 2257 3878 4890 2257
Size: 1621 1012 3185
Fragments arranged by size:
3185 1621 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5818
BstBI TT'CG_AA
Cuts at: 4622 4622
Size: 5818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstYI r'GATC_y
Cuts at: 1972 2198 3148 3515 3900 3917 5369 5455 5467
Size: 226 950 367 385 17 1452 86 12
Cuts at: 5467 5813 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 950 385 367 346 226 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2587 2587
Size: 5818
BtgI C'CryG_G
Cuts at: 513 1065 2004 2263 2766 2803 2850 2905 3056
Size: 552 939 259 503 37 47 55 151
Cuts at: 3056 513
Size: 3275
Fragments arranged by size:
3275 939 552 503 259 151 55 47
37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5079
Fragments arranged by size:
5079 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2864 3265 4154
Size: 54 1425 50 275 18 1034 401 889
Cuts at: 4154 5783 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1034 889 401 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2864 3265 1537
Size: 275 1052 401 4090
Fragments arranged by size:
4090 1052 401 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3748 1353
Size: 2395 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4405 4779 4925 387
Size: 1064 138 2816 374 146 1280
Fragments arranged by size:
2816 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 2498 2669 3065 3881 4478 5265
Size: 393 770 96 171 396 816 597 787
Cuts at: 5265 5533 1239
Size: 268 1524
Fragments arranged by size:
1524 816 787 770 597 396 393 268
171 96
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2651
Size: 5818
EcoO109I rG'GnC_Cy
Cuts at: 2012 3039 3040 2012
Size: 1027 1 4790
Fragments arranged by size:
4790 1027 1
EcoRI G'AATT_C
Cuts at: 2135 3127 3505 2135
Size: 992 378 4448
Fragments arranged by size:
4448 992 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 5818
FspI TGC'GCA
Cuts at: 2120 4324 2120
Size: 2204 3614
HaeII r_GCGC'y
Cuts at: 1678 2642 2883 3006 3055 3064 4965 1678
Size: 964 241 123 49 9 1901 2531
Fragments arranged by size:
2531 1901 964 241 123 49 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2689 2721 2741 2773 4459
Size: 32 135 32 1117 32 20 32 1686
Cuts at: 4459 4491 4533 4565 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1686 1117 135 42 32 32 32
32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 3162 3408 669
Size: 988 281 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2219 748
Size: 1471 4347
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KasI G'GCGC_C
Cuts at: 3002 3051 3060 3002
Size: 49 9 5760
Fragments arranged by size:
5760 49 9
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 2245 5694 2245
Size: 3449 2369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2268 2769 4932 5116 1305
Size: 963 501 2163 184 2007
Fragments arranged by size:
2163 2007 963 501 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3996
Fragments arranged by size:
3996 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 3776 4135 4294 518
Size: 648 948 1662 359 159 2042
Fragments arranged by size:
2042 1662 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2265 2284 2703 2768 2805 2852 3018 3234
Size: 659 19 419 65 37 47 166 216
Cuts at: 3234 3911 5059 5304 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 659 419 245 216 166
65 47 37 19
NarI GG'CG_CC
Cuts at: 3003 3052 3061 3003
Size: 49 9 5760
Fragments arranged by size:
5760 49 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4327
Fragments arranged by size:
4327 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5818
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 3176 4721 1132
Size: 97 1947 1545 2229
Fragments arranged by size:
2229 1947 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5818
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2476 1108
Size: 1368 4450
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PspOMI G'GGCC_C
Cuts at: 3039 3039
Size: 5818
PspXI vC'TCGA_Gb
Cuts at: 3075 3075
Size: 5818
PstI C_TGCA'G
Cuts at: 830 2283 2323 2505 3170 830
Size: 1453 40 182 665 3478
Fragments arranged by size:
3478 1453 665 182 40
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
PvuII CAG'CTG
Cuts at: 2284 2284
Size: 5818
SacII CC_GC'GG
Cuts at: 2266 2769 2806 2853 2266
Size: 503 37 47 5231
Fragments arranged by size:
5231 503 47 37
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 4066 5778 4066
Size: 1712 4106
SfcI C'TryA_G
Cuts at: 826 1045 2279 2319 2501 3166 4301 4982 5173
Size: 219 1234 40 182 665 1135 681 191
Cuts at: 5173 826
Size: 1471
Fragments arranged by size:
1471 1234 1135 681 665 219 191 182
40
SfoI GGC'GCC
Cuts at: 3004 3053 3062 3004
Size: 49 9 5760
Fragments arranged by size:
5760 49 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 2664 2936 3145 1872
Size: 792 272 209 4545
Fragments arranged by size:
4545 792 272 209
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 2159 3075 3922 4823 5085
Size: 197 926 204 12 916 847 901 262
Cuts at: 5085 820
Size: 1553
Fragments arranged by size:
1553 926 916 901 847 262 204 197
12
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 3176 1132
Size: 2044 3774
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 2536 1517
Size: 1019 4799
StyI C'CwwG_G
Cuts at: 513 1065 2004 2668 2692 3257 513
Size: 552 939 664 24 565 3074
Fragments arranged by size:
3074 939 664 565 552 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 3862 4015 4200 4645 325
Size: 1255 2265 17 153 185 445 1498
Fragments arranged by size:
2265 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4064 5776 96
Size: 274 80 33 51 1443 2087 1712 138
Fragments arranged by size:
2087 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2953 2985 5778 5810 2953
Size: 32 2793 32 2961
Fragments arranged by size:
2961 2793 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2473 2473
Size: 5818
XhoI C'TCGA_G
Cuts at: 1943 3075 1943
Size: 1132 4686
XmaI C'CCGG_G
Cuts at: 1870 2662 2934 3143 1870
Size: 792 272 209 4545
Fragments arranged by size:
4545 792 272 209
XmnI GAAnn'nnTTC
Cuts at: 3947 3947
Size: 5818
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI BaeI BbvCI BfrBI BplI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI