Restriction map for
pFN21AE0626
(Circular) (Six-base) MAPSORT of: pFN21AE0626.seq Check: 32 from: 1 to: 5821
pFN21AE0626 5821 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 3164 1937
Size: 870 357 4594
Fragments arranged by size:
4594 870 357
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3884 5268 5536 1632
Size: 2252 1384 268 1917
Fragments arranged by size:
2252 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 2149 4720 5697 1203
Size: 946 2571 977 1327
Fragments arranged by size:
2571 1327 977 946
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 2040 3564 5136 2040
Size: 1524 1572 2725
Fragments arranged by size:
2725 1572 1524
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 2081 2120 3059 3130 3356 3508 5617 1493
Size: 588 39 939 71 226 152 2109 1697
Fragments arranged by size:
2109 1697 939 588 226 152 71 39
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
AvrII C'CTAG_G
Cuts at: 2509 2509
Size: 5821
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3142 4597 618
Size: 325 217 627 1355 1455 1842
Fragments arranged by size:
1842 1455 1355 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3140 1391
Size: 1749 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2424 2877 2907 928
Size: 971 525 453 30 3842
Fragments arranged by size:
3842 971 525 453 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 2689 1548
Size: 1141 4680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2826 4432 136
Size: 107 122 71 631 605 1154 1606 1525
Fragments arranged by size:
1606 1525 1154 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 2978 3028 3238 2978
Size: 50 210 5561
Fragments arranged by size:
5561 210 50
Bme1580I G_kGCm'C
Cuts at: 1790 2051 3882 5038 1790
Size: 261 1831 1156 2573
Fragments arranged by size:
2573 1831 1156 261
BmgBI CAC'GTC
Cuts at: 1318 2446 1318
Size: 1128 4693
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2518 2975 3060 4481 1239
Size: 1279 457 85 1421 2579
Fragments arranged by size:
2579 1421 1279 457 85
Bpu10I CC'TnA_GC
Cuts at: 2270 2270
Size: 5821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2737 4484 882
Size: 617 1238 1747 2219
Fragments arranged by size:
2219 1747 1238 617
BsaAI yAC'GTr
Cuts at: 493 1204 3612 493
Size: 711 2408 2702
Fragments arranged by size:
2702 2408 711
BsaBI GATnn'nnATC
Cuts at: 2235 5815 2235
Size: 3580 2241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3628 4010 4645 275
Size: 53 83 186 936 2095 382 635 1451
Fragments arranged by size:
2095 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2798 4254 4652 4926 5073 5484 2798
Size: 1456 398 274 147 411 3135
Fragments arranged by size:
3135 1456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3058 3088 1178
Size: 30 165 30 1655 30 3911
Fragments arranged by size:
3911 1655 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 1186
Size: 955 4866
BseYI C'CCAG_C
Cuts at: 5024 5520 5024
Size: 496 5325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3882 3967 5038 3882
Size: 85 1071 4665
Fragments arranged by size:
4665 1071 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 3140 3882 3967 5038 1391
Size: 399 261 1089 742 85 1071 2174
Fragments arranged by size:
2174 1089 1071 742 399 261 85
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3176 844
Size: 2332 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 2746 4465 4652 1700
Size: 110 209 727 1719 187 2869
Fragments arranged by size:
2869 1719 727 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2040 794
Size: 1246 4575
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2160 2577 3151 3518 3903 3920 5372 5458
Size: 188 417 574 367 385 17 1452 86
Cuts at: 5458 5470 5816 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 574 417 385 367 346 188
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2200 513
Size: 552 1135 4134
Fragments arranged by size:
4134 1135 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2264 3337 4131 4151 2264
Size: 1073 794 20 3934
Fragments arranged by size:
3934 1073 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 3268 4157
Size: 54 1425 50 275 18 426 1012 889
Cuts at: 4157 5786 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1012 889 426 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2057 2675 3751 1353
Size: 704 618 1076 3423
Fragments arranged by size:
3423 1076 704 618
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2518 2975 3060 3884 4481 5268 5536
Size: 393 886 457 85 824 597 787 268
Cuts at: 5536 1239
Size: 1524
Fragments arranged by size:
1524 886 824 787 597 457 393 268
85
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 4968 1678
Size: 3290 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2505 2537 2674 2706 2764
Size: 32 135 32 933 32 137 32 58
Cuts at: 2764 2796 4462 4494 4536 4568 1373
Size: 32 1666 32 42 32 2626
Fragments arranged by size:
2626 1666 933 137 135 58 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 3165 3411 669
Size: 988 281 235 992 246 3079
Fragments arranged by size:
3079 992 988 281 246 235
HindIII A'AGCT_T
Cuts at: 748 2851 2980 748
Size: 2103 129 3589
Fragments arranged by size:
3589 2103 129
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2762 4935 5119 1305
Size: 1457 2173 184 2007
Fragments arranged by size:
2173 2007 1457 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 2738 3779 4138 4297 518
Size: 648 1239 333 1041 359 159 2042
Fragments arranged by size:
2042 1239 1041 648 359 333 159
MspA1I CmG'CkG
Cuts at: 1606 2037 3237 3914 5062 5307 1606
Size: 431 1200 677 1148 245 2120
Fragments arranged by size:
2120 1200 1148 677 431 245
NcoI C'CATG_G
Cuts at: 513 1065 2200 513
Size: 552 1135 4134
Fragments arranged by size:
4134 1135 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2153 3074 3179 4724 1132
Size: 97 924 921 105 1545 2229
Fragments arranged by size:
2229 1545 924 921 105 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 2149 4720 2149
Size: 2571 3250
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PfoI T'CCnGG_A
Cuts at: 2523 2523
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2428 1613
Size: 815 5006
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 2057 2675 2057
Size: 618 5203
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 2305 3169 4304 4985 5176 826
Size: 219 1260 864 1135 681 191 1471
Fragments arranged by size:
1471 1260 1135 864 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3925 4826 5088 820
Size: 197 926 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2200 2509 3260 513
Size: 552 1135 309 751 3074
Fragments arranged by size:
3074 1135 751 552 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2618 4067 5779
Size: 274 80 33 51 1443 641 1449 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1712 1449 1443 641 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5781 5813 5781
Size: 32 5789
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 2568 3950 2568
Size: 1382 4439
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI