Restriction map for
pFN21AE0624
(Circular) (Six-base) MAPSORT of: pFN21AE0624.seq Check: 8177 from: 1 to: 5815
pFN21AE0624 5815 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2586 3878 5262 5530 1632
Size: 954 1292 1384 268 1917
Fragments arranged by size:
1917 1384 1292 954 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5618
AflIII A'CryG_T
Cuts at: 1203 4714 5691 1203
Size: 3511 977 1327
Fragments arranged by size:
3511 1327 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 2936 1166
Size: 1770 4045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 2137 2380 3029 3558 5130 2137
Size: 243 649 529 1572 2822
Fragments arranged by size:
2822 1572 649 529 243
ApaLI G'TGCA_C
Cuts at: 3872 5028 3872
Size: 1156 4659
ApoI r'AATT_y
Cuts at: 1493 3124 3350 3502 5611 1493
Size: 1631 226 152 2109 1697
Fragments arranged by size:
2109 1697 1631 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 2515 3140 1870
Size: 73 572 625 4545
Fragments arranged by size:
4545 625 572 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2969 3136 4591 618
Size: 325 217 627 1182 167 1455 1842
Fragments arranged by size:
1842 1455 1182 627 325 217 167
BanII G_rGCy'C
Cuts at: 1391 2313 3134 1391
Size: 922 821 4072
Fragments arranged by size:
4072 922 821
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2472 928
Size: 971 573 4271
Fragments arranged by size:
4271 971 573
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2654 2688 3048 3082 4006
Size: 34 50 34 633 34 360 34 924
Cuts at: 4006 4040 1903
Size: 34 3678
Fragments arranged by size:
3678 924 633 360 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2546 3707 4917 2546
Size: 1161 1210 3444
Fragments arranged by size:
3444 1210 1161
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BfrBI ATG'CAT
Cuts at: 2154 2154
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 4426 136
Size: 107 122 71 631 605 493 2261 1525
Fragments arranged by size:
2261 1525 631 605 493 122 107 71
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2972 3083 3876 5032 1790
Size: 297 885 111 793 1156 2573
Fragments arranged by size:
2573 1156 885 793 297 111
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2737 3546 4504
Size: 416 53 83 399 24 1320 809 958
Cuts at: 4504 442
Size: 1753
Fragments arranged by size:
1753 1320 958 809 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 4475 1239
Size: 953 2283 2579
Fragments arranged by size:
2579 2283 953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 3940 4805 5103 2414
Size: 1526 865 298 3126
Fragments arranged by size:
3126 1526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2557 3606 493
Size: 711 1353 1049 2702
Fragments arranged by size:
2702 1353 1049 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2355 3622 4004 4639
Size: 53 83 186 936 822 1267 382 635
Cuts at: 4639 275
Size: 1451
Fragments arranged by size:
1451 1267 936 822 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5590
Fragments arranged by size:
5590 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5815
BseYI C'CCAG_C
Cuts at: 2008 2965 3029 5018 5514 2008
Size: 957 64 1989 496 2309
Fragments arranged by size:
2309 1989 957 496 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 1163
Size: 573 462 4780
Fragments arranged by size:
4780 573 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 2529 3876 3961 5032 2313
Size: 216 1347 85 1071 3096
Fragments arranged by size:
3096 1347 1071 216 85
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2313 2529 2972 3083 3134 3876
Size: 399 297 226 216 443 111 51 742
Cuts at: 3876 3961 5032 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 297 226 216
111 85 51
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 2478 3704 5434 2478
Size: 1226 1730 2859
Fragments arranged by size:
2859 1730 1226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2406 3170 844
Size: 1562 764 3489
Fragments arranged by size:
3489 1562 764
BsrBI CCG'CTC
Cuts at: 1394 1823 2350 3702 1394
Size: 429 527 1352 3507
Fragments arranged by size:
3507 1352 527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2154 4310 4484 65
Size: 1183 906 2156 174 1396
Fragments arranged by size:
2156 1396 1183 906 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3037 4459 4646 1700
Size: 110 1227 1422 187 2869
Fragments arranged by size:
2869 1422 1227 187 110
BsrGI T'GTAC_A
Cuts at: 96 2416 96
Size: 2320 3495
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2815 794
Size: 2021 3794
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 2645 1184
Size: 1461 4354
BstXI CCAn_nnnn'nTGG
Cuts at: 2938 2938
Size: 5815
BstYI r'GATC_y
Cuts at: 1972 2425 3145 3512 3897 3914 5366 5452 5464
Size: 453 720 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 720 453 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2825 2931 3004 513
Size: 552 1760 106 73 3324
Fragments arranged by size:
3324 1760 552 106 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2608 487
Size: 683 56 1382 3694
Fragments arranged by size:
3694 1382 683 56
BtsI GCAGTG_nn'
Cuts at: 2022 2527 3098 3331 4125 4145 2022
Size: 505 571 233 794 20 3692
Fragments arranged by size:
3692 794 571 505 233 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 3262 4151
Size: 54 1425 50 275 18 312 1120 889
Cuts at: 4151 5780 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1120 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3745 1353
Size: 2392 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2057 4402 4776 4922 387
Size: 1064 138 468 2345 374 146 1280
Fragments arranged by size:
2345 1280 1064 468 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 2311
Size: 5815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2586 3878 4475 5262 5530 1239
Size: 393 560 394 1292 597 787 268 1524
Fragments arranged by size:
1524 1292 787 597 560 394 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2024 2091 2167 2512 2782 2024
Size: 67 76 345 270 5057
Fragments arranged by size:
5057 345 270 76 67
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 2938 2911
Size: 27 5788
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 4962 1678
Size: 3284 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2594 2626 2723
Size: 32 135 32 881 32 109 32 97
Cuts at: 2723 2755 4456 4488 4530 4562 1373
Size: 32 1701 32 42 32 2626
Fragments arranged by size:
2626 1701 881 135 109 97 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3405 669
Size: 988 281 1221 246 3079
Fragments arranged by size:
3079 1221 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 3414 3414
Size: 5815
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2511 4929 5113 1305
Size: 1206 2418 184 2007
Fragments arranged by size:
2418 2007 1206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 10
Size: 54 1425 343 312 3681
Fragments arranged by size:
3681 1425 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2936 3773 4132 4291 518
Size: 648 1770 837 359 159 2042
Fragments arranged by size:
2042 1770 837 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2293 2965 3044 3231 3908 5056 5301 1606
Size: 687 672 79 187 677 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 672 245 187 79
NaeI GCC'GGC
Cuts at: 3039 3039
Size: 5815
NcoI C'CATG_G
Cuts at: 513 1065 2825 3004 513
Size: 552 1760 179 3324
Fragments arranged by size:
3324 1760 552 179
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NgoMIV G'CCGG_C
Cuts at: 3037 3037
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NsiI A_TGCA'T
Cuts at: 2156 2156
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 3173 4718 1132
Size: 97 1944 1545 2229
Fragments arranged by size:
2229 1944 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 4714 4714
Size: 5815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2380 3029 1108
Size: 1272 649 3894
Fragments arranged by size:
3894 1272 649
PfoI T'CCnGG_A
Cuts at: 2548 2548
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2512 2782 2512
Size: 270 5545
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PstI C_TGCA'G
Cuts at: 830 2964 3167 830
Size: 2134 203 3478
Fragments arranged by size:
3478 2134 203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
PvuII CAG'CTG
Cuts at: 2293 2965 3044 2293
Size: 672 79 5064
Fragments arranged by size:
5064 672 79
SacI G_AGCT'C
Cuts at: 2313 2313
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 2452 2587 2960 3163 4298 4979 5170
Size: 219 1407 135 373 203 1135 681 191
Cuts at: 5170 826
Size: 1471
Fragments arranged by size:
1471 1407 1135 681 373 219 203 191
135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 2517 3142 1872
Size: 645 625 4545
Fragments arranged by size:
4545 645 625
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 3919 4820 5082 820
Size: 197 926 486 1490 901 262 1553
Fragments arranged by size:
1553 1490 926 901 486 262 197
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 3739 5
Size: 47 3687 2081
Fragments arranged by size:
3687 2081 47
StuI AGG'CCT
Cuts at: 1517 2813 1517
Size: 1296 4519
StyI C'CwwG_G
Cuts at: 513 1065 2160 2825 3004 3254 513
Size: 552 1095 665 179 250 3074
Fragments arranged by size:
3074 1095 665 552 250 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2838 3842 3859 4012 4197 4642 325
Size: 1255 1258 1004 17 153 185 445 1498
Fragments arranged by size:
1498 1258 1255 1004 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2416 2448 4061
Size: 274 80 33 51 1443 439 32 1613
Cuts at: 4061 5773 96
Size: 1712 138
Fragments arranged by size:
1712 1613 1443 439 274 138 80 51
33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 5783
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 2848 3151 2848
Size: 303 5512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2167
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 2515 3140 1870
Size: 645 625 4545
Fragments arranged by size:
4545 645 625
XmnI GAAnn'nnTTC
Cuts at: 2795 3944 2795
Size: 1149 4666
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NarI NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI