Restriction map for
pFN21AE0618
(Circular) (Six-base) MAPSORT of: pFN21AE0618.seq Check: 2661 from: 1 to: 5803
pFN21AE0618 5803 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3613 278
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 1937
Size: 1209 4594
Acc65I G'GTAC_C
Cuts at: 3124 3124
Size: 5803
AclI AA'CG_TT
Cuts at: 1563 3930 4303 1563
Size: 2367 373 3063
Fragments arranged by size:
3063 2367 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3866 5250 5518 1632
Size: 2234 1384 268 1917
Fragments arranged by size:
2234 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5606
AflIII A'CryG_T
Cuts at: 1203 4702 5679 1203
Size: 3499 977 1327
Fragments arranged by size:
3499 1327 977
AgeI A'CCGG_T
Cuts at: 4634 4634
Size: 5803
AhdI GACnn_n'nnGTC
Cuts at: 2754 4532 2754
Size: 1778 4025
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3111 3114 1184
Size: 1927 3 3873
Fragments arranged by size:
3873 1927 3
AlwNI CAG_nnn'CTG
Cuts at: 2272 2680 2887 3546 5118 2272
Size: 408 207 659 1572 2957
Fragments arranged by size:
2957 1572 659 408 207
ApaI G_GGCC'C
Cuts at: 2368 2368
Size: 5803
ApaLI G'TGCA_C
Cuts at: 3860 5016 3860
Size: 1156 4647
ApoI r'AATT_y
Cuts at: 1493 2151 3112 3338 3490 5599 1493
Size: 658 961 226 152 2109 1697
Fragments arranged by size:
2109 1697 961 658 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4357 160
Size: 872 3325 1606
Fragments arranged by size:
3325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
AvaI C'yCGr_G
Cuts at: 1870 1943 2361 3128 1870
Size: 73 418 767 4545
Fragments arranged by size:
4545 767 418 73
BamHI G'GATC_C
Cuts at: 3133 3500 3133
Size: 367 5436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3124 4579 618
Size: 325 217 627 1337 1455 1842
Fragments arranged by size:
1842 1455 1337 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2240 2368 2732 3122 1391
Size: 849 128 364 390 4072
Fragments arranged by size:
4072 849 390 364 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2627 928
Size: 971 728 4104
Fragments arranged by size:
4104 971 728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3994 4028 1903
Size: 34 2057 34 3678
Fragments arranged by size:
3678 2057 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3695 4905 3695
Size: 1210 4593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4414 136
Size: 107 122 71 631 605 2742 1525
Fragments arranged by size:
2742 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2230 5798 2230
Size: 3568 2235
BlpI GC'TnA_GC
Cuts at: 3220 3220
Size: 5803
Bme1580I G_kGCm'C
Cuts at: 1790 2368 3864 5020 1790
Size: 578 1496 1156 2573
Fragments arranged by size:
2573 1496 1156 578
BmgBI CAC'GTC
Cuts at: 1318 2647 1318
Size: 1329 4474
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3534 4492 442
Size: 416 53 83 399 24 2117 958 1753
Fragments arranged by size:
2117 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2067 2744 2969 4463 1239
Size: 828 677 225 1494 2579
Fragments arranged by size:
2579 1494 828 677 225
Bpu10I CC'TnA_GC
Cuts at: 2552 2697 2552
Size: 145 5658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3928 4793 5091 3928
Size: 865 298 4640
Fragments arranged by size:
4640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2541 4466 882
Size: 617 1042 1925 2219
Fragments arranged by size:
2219 1925 1042 617
BsaAI yAC'GTr
Cuts at: 493 1204 3594 493
Size: 711 2390 2702
Fragments arranged by size:
2702 2390 711
BsaBI GATnn'nnATC
Cuts at: 5797 5797
Size: 5803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3610 3992 4627 275
Size: 53 83 186 936 2077 382 635 1451
Fragments arranged by size:
2077 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4236 4634 4908 5055 5466 4236
Size: 398 274 147 411 4573
Fragments arranged by size:
4573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5578
Fragments arranged by size:
5578 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2720 1186
Size: 898 636 4269
Fragments arranged by size:
4269 898 636
BseYI C'CCAG_C
Cuts at: 5006 5502 5006
Size: 496 5307
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2645 2900 1163
Size: 573 909 255 4066
Fragments arranged by size:
4066 909 573 255
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3253 4014 5042 1540
Size: 275 186 1252 761 1028 2301
Fragments arranged by size:
2301 1252 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3864 3949 5020 3864
Size: 85 1071 4647
Fragments arranged by size:
4647 1071 85
BsmI GAATG_Cn'
Cuts at: 2543 3314 3407 2543
Size: 771 93 4939
Fragments arranged by size:
4939 771 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2368 2732 3122 3864 3949 5020
Size: 399 450 128 364 390 742 85 1071
Cuts at: 5020 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 450 399 390 364 128
85
BspEI T'CCGG_A
Cuts at: 5466 5466
Size: 5803
BspHI T'CATG_A
Cuts at: 3692 5422 3692
Size: 1730 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3158 844
Size: 2314 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3690 1394
Size: 429 1867 3507
Fragments arranged by size:
3507 1867 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4298 4472 65
Size: 1183 3050 174 1396
Fragments arranged by size:
3050 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4447 4634 1700
Size: 110 2637 187 2869
Fragments arranged by size:
2869 2637 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5803
BssSI C'ACGA_G
Cuts at: 3863 4875 3863
Size: 1012 4791
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2887 794
Size: 2093 3710
BstBI TT'CG_AA
Cuts at: 4607 4607
Size: 5803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5803
BstYI r'GATC_y
Cuts at: 1972 2230 3133 3500 3885 3902 5354 5440 5452
Size: 258 903 367 385 17 1452 86 12
Cuts at: 5452 5798 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 903 385 367 346 258 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2531 2531
Size: 5803
BtgI C'CryG_G
Cuts at: 513 1065 2581 513
Size: 552 1516 3735
Fragments arranged by size:
3735 1516 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5064
Fragments arranged by size:
5064 683 56
BtsI GCAGTG_nn'
Cuts at: 3319 4113 4133 3319
Size: 794 20 4989
Fragments arranged by size:
4989 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5768
DraI TTT'AAA
Cuts at: 3107 3107
Size: 5803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4810 809
Size: 507 3494 1802
Fragments arranged by size:
3494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2865 3250 4139
Size: 54 1425 50 275 18 1035 385 889
Cuts at: 4139 5768 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1035 889 385 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3250 1537
Size: 275 1438 4090
Fragments arranged by size:
4090 1438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 3733 1353
Size: 938 1442 3423
Fragments arranged by size:
3423 1442 938
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2717 4390 4764 4910 387
Size: 1064 138 1128 1673 374 146 1280
Fragments arranged by size:
1673 1280 1128 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2744 2969 3866 4463 5250 5518
Size: 393 435 677 225 897 597 787 268
Cuts at: 5518 1239
Size: 1524
Fragments arranged by size:
1524 897 787 677 597 435 393 268
225
EcoNI CCTnn'n_nnAGG
Cuts at: 2165 2696 2797 2165
Size: 531 101 5171
Fragments arranged by size:
5171 531 101
EcoO109I rG'GnC_Cy
Cuts at: 2325 2337 2365 2511 2325
Size: 12 28 146 5617
Fragments arranged by size:
5617 146 28 12
EcoRI G'AATT_C
Cuts at: 3112 3490 3112
Size: 378 5425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2273 2273
Size: 5803
FspI TGC'GCA
Cuts at: 2891 4309 2891
Size: 1418 4385
HaeII r_GCGC'y
Cuts at: 1678 4950 1678
Size: 3272 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 2222 2254 2740
Size: 32 135 32 491 32 127 32 486
Cuts at: 2740 2772 4444 4476 4518 4550 1373
Size: 32 1672 32 42 32 2626
Fragments arranged by size:
2626 1672 491 486 135 127 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2449 3147 3393 669
Size: 988 281 511 698 246 3079
Fragments arranged by size:
3079 988 698 511 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5803
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 5803
KpnI G_GTAC'C
Cuts at: 3128 3128
Size: 5803
MfeI C'AATT_G
Cuts at: 3402 3402
Size: 5803
MluI A'CGCG_T
Cuts at: 5679 5679
Size: 5803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2046 2358 2478 4917 5101 1305
Size: 741 312 120 2439 184 2007
Fragments arranged by size:
2439 2007 741 312 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2867 10
Size: 54 1425 343 1035 2946
Fragments arranged by size:
2946 1425 1035 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 2876 3761 4120 4279 518
Size: 648 1572 138 885 359 159 2042
Fragments arranged by size:
2042 1572 885 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2371 3219 3896 5044 5289 1606
Size: 765 848 677 1148 245 2120
Fragments arranged by size:
2120 1148 848 765 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5251
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5803
NotI GC'GGCC_GC
Cuts at: 3250 3250
Size: 5803
NspI r_CATG'y
Cuts at: 1132 1229 2674 3161 4706 1132
Size: 97 1445 487 1545 2229
Fragments arranged by size:
2229 1545 1445 487 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5803
PciI A'CATG_T
Cuts at: 4702 4702
Size: 5803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4901
PfoI T'CCnGG_A
Cuts at: 2009 2083 2265 2009
Size: 74 182 5547
Fragments arranged by size:
5547 182 74
PmeI GTTT'AAAC
Cuts at: 3107 3107
Size: 5803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 5385 5417 3872
Size: 32 1481 32 4258
Fragments arranged by size:
4258 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2337 2511 2337
Size: 174 5629
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5803
PsiI TTA'TAA
Cuts at: 3373 3373
Size: 5803
PspOMI G'GGCC_C
Cuts at: 2364 2364
Size: 5803
PstI C_TGCA'G
Cuts at: 830 3090 3155 830
Size: 2260 65 3478
Fragments arranged by size:
3478 2260 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5803
SalI G'TCGA_C
Cuts at: 1936 3145 1936
Size: 1209 4594
SbfI CC_TGCA'GG
Cuts at: 3155 3155
Size: 5803
ScaI AGT'ACT
Cuts at: 4051 5763 4051
Size: 1712 4091
SfcI C'TryA_G
Cuts at: 826 1045 3086 3151 4286 4967 5158 826
Size: 219 2041 65 1135 681 191 1471
Fragments arranged by size:
2041 1471 1135 681 219 191 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5803
SmaI CCC'GGG
Cuts at: 1872 3130 1872
Size: 1258 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3907 4808 5070 820
Size: 197 926 1964 901 262 1553
Fragments arranged by size:
1964 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3594 493
Size: 3101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5803
SphI G_CATG'C
Cuts at: 1132 3161 1132
Size: 2029 3774
SspI AAT'ATT
Cuts at: 5 52 3727 5
Size: 47 3675 2081
Fragments arranged by size:
3675 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5803
StyI C'CwwG_G
Cuts at: 513 1065 3075 3242 513
Size: 552 2010 167 3074
Fragments arranged by size:
3074 2010 552 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2797 3830 3847 4000 4185 4630 325
Size: 1255 1217 1033 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1217 1033 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4049 5761 96
Size: 274 80 33 51 1443 2072 1712 138
Fragments arranged by size:
2072 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2247 2279 5763 5795 2247
Size: 32 3484 32 2255
Fragments arranged by size:
3484 2255 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5803
XbaI T'CTAG_A
Cuts at: 3139 3139
Size: 5803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2273 2579 2273
Size: 306 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5803
XmaI C'CCGG_G
Cuts at: 1870 3128 1870
Size: 1258 4545
XmnI GAAnn'nnTTC
Cuts at: 3932 3932
Size: 5803
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3611 276
Size: 53 83 186 936 2077 2468
Fragments arranged by size:
2468 2077 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI