Restriction map for
pFN21AE0610
(Circular) (Six-base) MAPSORT of: pFN21AE0610.seq Check: 1190 from: 1 to: 5794
pFN21AE0610 5794 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2880 2880
Size: 5794
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3030 3921 4294 1563
Size: 1467 891 373 3063
Fragments arranged by size:
3063 1467 891 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3857 5241 5509 1632
Size: 2225 1384 268 1917
Fragments arranged by size:
2225 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 4693 5670 1203
Size: 3490 977 1327
Fragments arranged by size:
3490 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5794
AlwNI CAG_nnn'CTG
Cuts at: 2516 3537 5109 2516
Size: 1021 1572 3201
Fragments arranged by size:
3201 1572 1021
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 2230 3103 3329 3481 5590 1493
Size: 737 873 226 152 2109 1697
Fragments arranged by size:
2109 1697 873 737 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2987 4348 160
Size: 872 1955 1361 1606
Fragments arranged by size:
1955 1606 1361 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 1870
Size: 73 1176 4545
Fragments arranged by size:
4545 1176 73
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3115 4570 618
Size: 325 217 627 1328 1455 1842
Fragments arranged by size:
1842 1455 1328 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3113 1391
Size: 1722 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3985 4019 1903
Size: 34 2048 34 3678
Fragments arranged by size:
3678 2048 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 2224 1548
Size: 676 5118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4405 136
Size: 107 122 71 631 605 2733 1525
Fragments arranged by size:
2733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5789 5789
Size: 5794
BlpI GC'TnA_GC
Cuts at: 3211 3211
Size: 5794
Bme1580I G_kGCm'C
Cuts at: 1790 3855 5011 1790
Size: 2065 1156 2573
Fragments arranged by size:
2573 2065 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2389 3525 4483
Size: 416 53 83 399 24 972 1136 958
Cuts at: 4483 442
Size: 1753
Fragments arranged by size:
1753 1136 972 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2464 2509 2562 4454 1239
Size: 1225 45 53 1892 2579
Fragments arranged by size:
2579 1892 1225 53 45
Bpu10I CC'TnA_GC
Cuts at: 2887 2887
Size: 5794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2607 3919 4784 5082 2607
Size: 1312 865 298 3319
Fragments arranged by size:
3319 1312 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2389 2549 2746 2843 4457 882
Size: 617 890 160 197 97 1614 2219
Fragments arranged by size:
2219 1614 890 617 197 160 97
BsaAI yAC'GTr
Cuts at: 493 1204 3585 493
Size: 711 2381 2702
Fragments arranged by size:
2702 2381 711
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3601 3983 4618 275
Size: 53 83 186 936 2068 382 635 1451
Fragments arranged by size:
2068 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4227 4625 4899 5046 5457 4227
Size: 398 274 147 411 4564
Fragments arranged by size:
4564 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 1178
Size: 30 165 30 966 30 4573
Fragments arranged by size:
4573 966 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2104 2453 2646 3033 1186
Size: 918 349 193 387 3947
Fragments arranged by size:
3947 918 387 349 193
BseYI C'CCAG_C
Cuts at: 2159 4997 5493 2159
Size: 2838 496 2460
Fragments arranged by size:
2838 2460 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 1163
Size: 573 564 4657
Fragments arranged by size:
4657 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3244 4005 5033 1540
Size: 275 186 1243 761 1028 2301
Fragments arranged by size:
2301 1243 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3855 3940 5011 3855
Size: 85 1071 4638
Fragments arranged by size:
4638 1071 85
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3113 3855 3940 5011 1391
Size: 399 1323 742 85 1071 2174
Fragments arranged by size:
2174 1323 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 3683 5413 3683
Size: 1730 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2880 3149 844
Size: 2036 269 3489
Fragments arranged by size:
3489 2036 269
BsrBI CCG'CTC
Cuts at: 1394 1823 3681 1394
Size: 429 1858 3507
Fragments arranged by size:
3507 1858 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2688 4289 4463 65
Size: 1183 1440 1601 174 1396
Fragments arranged by size:
1601 1440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4438 4625 1700
Size: 110 2628 187 2869
Fragments arranged by size:
2869 2628 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 3854 4866 3854
Size: 1012 4782
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2278 794
Size: 1484 4310
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstXI CCAn_nnnn'nTGG
Cuts at: 2744 2744
Size: 5794
BstYI r'GATC_y
Cuts at: 1972 3124 3491 3876 3893 5345 5431 5443 5789
Size: 1152 367 385 17 1452 86 12 346
Cuts at: 5789 1972
Size: 1977
Fragments arranged by size:
1977 1452 1152 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4303
Fragments arranged by size:
4303 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 3310 4104 4124 3310
Size: 794 20 4980
Fragments arranged by size:
4980 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 2293 3098 2293
Size: 805 4989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4801 809
Size: 507 3485 1802
Fragments arranged by size:
3485 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2353 3241 4130
Size: 54 1425 50 275 18 523 888 889
Cuts at: 4130 5759 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 888 523 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3241 1537
Size: 275 1429 4090
Fragments arranged by size:
4090 1429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2477 2631 3724 1353
Size: 665 459 154 1093 3423
Fragments arranged by size:
3423 1093 665 459 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4381 4755 4901 387
Size: 1064 138 2792 374 146 1280
Fragments arranged by size:
2792 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2464 2509 2562 3857 4454 5241 5509
Size: 393 832 45 53 1295 597 787 268
Cuts at: 5509 1239
Size: 1524
Fragments arranged by size:
1524 1295 832 787 597 393 268 53
45
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 5794
EcoO109I rG'GnC_Cy
Cuts at: 2863 2863
Size: 5794
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 4941 1678
Size: 3263 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 2300 2332 2369
Size: 32 135 32 589 32 107 32 37
Cuts at: 2369 2401 2726 2758 4435 4467 4509 4541 1373
Size: 32 325 32 1677 32 42 32 2626
Fragments arranged by size:
2626 1677 589 325 135 107 42 37
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3138 3384 669
Size: 988 281 1200 246 3079
Fragments arranged by size:
3079 1200 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 2975 3393 2975
Size: 418 5376
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2658 4908 5092 1305
Size: 1353 2250 184 2007
Fragments arranged by size:
2250 2007 1353 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2355 10
Size: 54 1425 343 523 3449
Fragments arranged by size:
3449 1425 523 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2304 3752 4111 4270 518
Size: 648 1138 1448 359 159 2042
Fragments arranged by size:
2042 1448 1138 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 3210 3887 5035 5280 1606
Size: 501 1103 677 1148 245 2120
Fragments arranged by size:
2120 1148 1103 677 501 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4303
Fragments arranged by size:
4303 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 3152 4697 1132
Size: 97 1923 1545 2229
Fragments arranged by size:
2229 1923 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5794
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2540 1108
Size: 1432 4362
PfoI T'CCnGG_A
Cuts at: 2578 2578
Size: 5794
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2863 2863
Size: 5794
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 2190 3364 2190
Size: 1174 4620
PstI C_TGCA'G
Cuts at: 830 3146 830
Size: 2316 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
PvuII CAG'CTG
Cuts at: 2107 2107
Size: 5794
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SexAI A'CCwGG_T
Cuts at: 2736 2736
Size: 5794
SfcI C'TryA_G
Cuts at: 826 1045 3142 4277 4958 5149 826
Size: 219 2097 1135 681 191 1471
Fragments arranged by size:
2097 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2622 3898 4799 5061 820
Size: 197 926 679 1276 901 262 1553
Fragments arranged by size:
1553 1276 926 901 679 262 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 3152 1132
Size: 2020 3774
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5794
StyI C'CwwG_G
Cuts at: 513 1065 2004 2491 3233 513
Size: 552 939 487 742 3074
Fragments arranged by size:
3074 939 742 552 487
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3821 3838 3991 4176 4621 325
Size: 1255 2241 17 153 185 445 1498
Fragments arranged by size:
2241 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4040 5752 96
Size: 274 80 33 51 1443 2063 1712 138
Fragments arranged by size:
2063 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2195 2227 5754 5786 2195
Size: 32 3527 32 2203
Fragments arranged by size:
3527 2203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2381 2738 1316
Size: 1065 357 4372
Fragments arranged by size:
4372 1065 357
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 3032 3923 3032
Size: 891 4903
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI