Restriction map for
pFN21AE0609
(Circular) (Six-base) MAPSORT of: pFN21AE0609.seq Check: 6134 from: 1 to: 5218
pFN21AE0609 5218 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2231 2231
Size: 5218
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3028 278
Size: 53 83 186 936 1492 2468
Fragments arranged by size:
2468 1492 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2561 1937
Size: 624 4594
Acc65I G'GTAC_C
Cuts at: 2539 2539
Size: 5218
AclI AA'CG_TT
Cuts at: 1563 3345 3718 1563
Size: 1782 373 3063
Fragments arranged by size:
3063 1782 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2161 2369 3281 4665 4933 1632
Size: 529 208 912 1384 268 1917
Fragments arranged by size:
1917 1384 912 529 268 208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5021
AflIII A'CryG_T
Cuts at: 1203 2040 2378 4117 5094 1203
Size: 837 338 1739 977 1327
Fragments arranged by size:
1739 1327 977 837 338
AgeI A'CCGG_T
Cuts at: 4049 4049
Size: 5218
AhdI GACnn_n'nnGTC
Cuts at: 3947 3947
Size: 5218
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5218
AlwNI CAG_nnn'CTG
Cuts at: 2077 2961 4533 2077
Size: 884 1572 2762
Fragments arranged by size:
2762 1572 884
ApaLI G'TGCA_C
Cuts at: 3275 4431 3275
Size: 1156 4062
ApoI r'AATT_y
Cuts at: 1493 2408 2527 2753 2905 5014 1493
Size: 915 119 226 152 2109 1697
Fragments arranged by size:
2109 1697 915 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 3772 160
Size: 872 2740 1606
Fragments arranged by size:
2740 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5218
AvaI C'yCGr_G
Cuts at: 1870 1943 2543 1870
Size: 73 600 4545
Fragments arranged by size:
4545 600 73
BamHI G'GATC_C
Cuts at: 2548 2915 2548
Size: 367 4851
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2539 3994 618
Size: 325 217 627 752 1455 1842
Fragments arranged by size:
1842 1455 752 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2537 1391
Size: 1146 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3409 3443 1903
Size: 34 1472 34 3678
Fragments arranged by size:
3678 1472 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3110 4320 3110
Size: 1210 4008
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5218
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3829 136
Size: 107 122 71 631 605 2157 1525
Fragments arranged by size:
2157 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5213 5213
Size: 5218
BlpI GC'TnA_GC
Cuts at: 2635 2635
Size: 5218
Bme1580I G_kGCm'C
Cuts at: 1790 3279 4435 1790
Size: 1489 1156 2573
Fragments arranged by size:
2573 1489 1156
BmgBI CAC'GTC
Cuts at: 1318 2041 1318
Size: 723 4495
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2949 3907 442
Size: 416 53 83 399 24 1532 958 1753
Fragments arranged by size:
1753 1532 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 3878 1239
Size: 842 1797 2579
Fragments arranged by size:
2579 1797 842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 3343 4208 4506 2138
Size: 1205 865 298 2850
Fragments arranged by size:
2850 1205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3881 882
Size: 617 2382 2219
Fragments arranged by size:
2382 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3009 493
Size: 711 1805 2702
Fragments arranged by size:
2702 1805 711
BsaBI GATnn'nnATC
Cuts at: 5212 5212
Size: 5218
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3025 3407 4042 275
Size: 53 83 186 936 1492 382 635 1451
Fragments arranged by size:
1492 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3651 4049 4323 4470 4881 3651
Size: 398 274 147 411 3988
Fragments arranged by size:
3988 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4993
Fragments arranged by size:
4993 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 4326
BseYI C'CCAG_C
Cuts at: 4421 4917 4421
Size: 496 4722
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2259 1163
Size: 573 523 4122
Fragments arranged by size:
4122 573 523
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2668 3429 4457 1540
Size: 275 186 667 761 1028 2301
Fragments arranged by size:
2301 1028 761 667 275 186
BsiHKAI G_wGCw'C
Cuts at: 2405 3279 3364 4435 2405
Size: 874 85 1071 3188
Fragments arranged by size:
3188 1071 874 85
BsmI GAATG_Cn'
Cuts at: 2327 2459 2729 2822 2327
Size: 132 270 93 4723
Fragments arranged by size:
4723 270 132 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2405 2537 3279 3364 4435 1391
Size: 399 615 132 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 615 399 132 85
BspEI T'CCGG_A
Cuts at: 4881 4881
Size: 5218
BspHI T'CATG_A
Cuts at: 3107 4837 3107
Size: 1730 3488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2231 2573 844
Size: 1387 342 3489
Fragments arranged by size:
3489 1387 342
BsrBI CCG'CTC
Cuts at: 1394 1823 3105 1394
Size: 429 1282 3507
Fragments arranged by size:
3507 1282 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3713 3887 65
Size: 1183 2465 174 1396
Fragments arranged by size:
2465 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3862 4049 1700
Size: 110 2052 187 2869
Fragments arranged by size:
2869 2052 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5218
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5218
BssSI C'ACGA_G
Cuts at: 3278 4290 3278
Size: 1012 4206
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5218
BstBI TT'CG_AA
Cuts at: 4022 4022
Size: 5218
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5218
BstXI CCAn_nnnn'nTGG
Cuts at: 2262 2262
Size: 5218
BstYI r'GATC_y
Cuts at: 1972 2548 2915 3300 3317 4769 4855 4867 5213
Size: 576 367 385 17 1452 86 12 346
Cuts at: 5213 1972
Size: 1977
Fragments arranged by size:
1977 1452 576 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2142 513
Size: 552 939 138 3589
Fragments arranged by size:
3589 939 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4479
Fragments arranged by size:
4479 683 56
BtsI GCAGTG_nn'
Cuts at: 2734 3528 3548 2734
Size: 794 20 4404
Fragments arranged by size:
4404 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5218
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5183
DraI TTT'AAA
Cuts at: 2476 2522 2476
Size: 46 5172
DraIII CAC_nnn'GTG
Cuts at: 2137 2137
Size: 5218
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4225 809
Size: 507 2909 1802
Fragments arranged by size:
2909 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2665 3554
Size: 54 1425 50 275 18 177 658 889
Cuts at: 3554 5183 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 658 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2665 1537
Size: 275 853 4090
Fragments arranged by size:
4090 853 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 3148 1353
Size: 837 958 3423
Fragments arranged by size:
3423 958 837
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3805 4179 4325 387
Size: 1064 138 2216 374 146 1280
Fragments arranged by size:
2216 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2161 2369 3281 3878 4665 4933
Size: 393 449 80 208 912 597 787 268
Cuts at: 4933 1239
Size: 1524
Fragments arranged by size:
1524 912 787 597 449 393 268 208
80
EcoO109I rG'GnC_Cy
Cuts at: 2220 2220
Size: 5218
EcoRI G'AATT_C
Cuts at: 2527 2905 2527
Size: 378 4840
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2327 2327
Size: 5218
FspI TGC'GCA
Cuts at: 3724 3724
Size: 5218
HaeII r_GCGC'y
Cuts at: 1678 4365 1678
Size: 2687 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3859 3891 3933 3965 1373
Size: 32 135 32 2287 32 42 32 2626
Fragments arranged by size:
2626 2287 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2562 2808 669
Size: 988 281 624 246 3079
Fragments arranged by size:
3079 988 624 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5218
HpaI GTT'AAC
Cuts at: 2808 2808
Size: 5218
KpnI G_GTAC'C
Cuts at: 2543 2543
Size: 5218
MfeI C'AATT_G
Cuts at: 2817 2817
Size: 5218
MluI A'CGCG_T
Cuts at: 5094 5094
Size: 5218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4332 4516 1305
Size: 3027 184 2007
Fragments arranged by size:
3027 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3219
Fragments arranged by size:
3219 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3176 3535 3694 518
Size: 648 843 1167 359 159 2042
Fragments arranged by size:
2042 1167 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2634 3311 4459 4704 1606
Size: 1028 677 1148 245 2120
Fragments arranged by size:
2120 1148 1028 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3727
Fragments arranged by size:
3727 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5218
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5218
NotI GC'GGCC_GC
Cuts at: 2665 2665
Size: 5218
NspI r_CATG'y
Cuts at: 1132 1229 2382 2576 4121 1132
Size: 97 1153 194 1545 2229
Fragments arranged by size:
2229 1545 1153 194 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5218
PciI A'CATG_T
Cuts at: 2378 4117 2378
Size: 1739 3479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5218
PfoI T'CCnGG_A
Cuts at: 2201 2201
Size: 5218
PmeI GTTT'AAAC
Cuts at: 2522 2522
Size: 5218
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 4800 4832 3287
Size: 32 1481 32 3673
Fragments arranged by size:
3673 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2220 2220
Size: 5218
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5218
PsiI TTA'TAA
Cuts at: 2788 2788
Size: 5218
PstI C_TGCA'G
Cuts at: 830 2570 830
Size: 1740 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5218
SalI G'TCGA_C
Cuts at: 1936 2560 1936
Size: 624 4594
SanDI GG'GwC_CC
Cuts at: 2220 2220
Size: 5218
SbfI CC_TGCA'GG
Cuts at: 2570 2570
Size: 5218
ScaI AGT'ACT
Cuts at: 3466 5178 3466
Size: 1712 3506
SexAI A'CCwGG_T
Cuts at: 2133 2188 2133
Size: 55 5163
SfcI C'TryA_G
Cuts at: 826 1045 2101 2125 2566 3701 4382 4573 826
Size: 219 1056 24 441 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 681 441 219 191 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5218
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5218
SmaI CCC'GGG
Cuts at: 1872 2545 1872
Size: 673 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 3322 4223 4485 820
Size: 197 926 174 1205 901 262 1553
Fragments arranged by size:
1553 1205 926 901 262 197 174
SnaBI TAC'GTA
Cuts at: 493 3009 493
Size: 2516 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5218
SphI G_CATG'C
Cuts at: 1132 2576 1132
Size: 1444 3774
SspI AAT'ATT
Cuts at: 5 52 2441 3142 5
Size: 47 2389 701 2081
Fragments arranged by size:
2389 2081 701 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5218
StyI C'CwwG_G
Cuts at: 513 1065 2004 2315 2657 513
Size: 552 939 311 342 3074
Fragments arranged by size:
3074 939 552 342 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3245 3262 3415 3600 4045 325
Size: 1255 1665 17 153 185 445 1498
Fragments arranged by size:
1665 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3464 5176 96
Size: 274 80 33 51 1443 1487 1712 138
Fragments arranged by size:
1712 1487 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 5178 5210 2123
Size: 32 3023 32 2131
Fragments arranged by size:
3023 2131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5218
XbaI T'CTAG_A
Cuts at: 2554 2554
Size: 5218
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5218
XmaI C'CCGG_G
Cuts at: 1870 2543 1870
Size: 673 4545
XmnI GAAnn'nnTTC
Cuts at: 3347 3347
Size: 5218
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3026 276
Size: 53 83 186 936 1492 2468
Fragments arranged by size:
2468 1492 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SfiI SfoI SrfI SwaI
XcmI