Restriction map for
pFN21AE0608
(Circular) (Six-base) MAPSORT of: pFN21AE0608.seq Check: 5336 from: 1 to: 5782
pFN21AE0608 5782 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2809 3845 5229 5497 1632
Size: 1177 1036 1384 268 1917
Fragments arranged by size:
1917 1384 1177 1036 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 2792 4681 5658 1203
Size: 1589 1889 977 1327
Fragments arranged by size:
1889 1589 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2647 1184
Size: 1463 4319
AlwNI CAG_nnn'CTG
Cuts at: 3525 5097 3525
Size: 1572 4210
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 2277 3032 3091 3317 3469 5578 1493
Size: 784 755 59 226 152 2109 1697
Fragments arranged by size:
2109 1697 784 755 226 152 59
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2163 3107 1870
Size: 73 220 944 4545
Fragments arranged by size:
4545 944 220 73
BamHI G'GATC_C
Cuts at: 2936 3112 3479 2936
Size: 176 367 5239
Fragments arranged by size:
5239 367 176
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2819 3103 4558 618
Size: 325 217 627 1032 284 1455 1842
Fragments arranged by size:
1842 1455 1032 627 325 284 217
BanII G_rGCy'C
Cuts at: 1391 2759 3101 1391
Size: 1368 342 4072
Fragments arranged by size:
4072 1368 342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 2454 1548
Size: 906 4876
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2582 5777 2582
Size: 3195 2587
BlpI GC'TnA_GC
Cuts at: 2753 3199 2753
Size: 446 5336
Bme1580I G_kGCm'C
Cuts at: 1790 3843 4999 1790
Size: 2053 1156 2573
Fragments arranged by size:
2573 2053 1156
BmgBI CAC'GTC
Cuts at: 1318 2793 1318
Size: 1475 4307
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2631 3513 4471
Size: 416 53 83 399 24 1214 882 958
Cuts at: 4471 442
Size: 1753
Fragments arranged by size:
1753 1214 958 882 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2141 1056
Size: 1085 4697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2794 4442 1239
Size: 1555 1648 2579
Fragments arranged by size:
2579 1648 1555
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2226 4445 882
Size: 617 727 2219 2219
Fragments arranged by size:
2219 2219 727 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2465 2712 2910 4215 4613 4887 5034 5445 2465
Size: 247 198 1305 398 274 147 411 2802
Fragments arranged by size:
2802 1305 411 398 274 247 198 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2004 2034 2905 2935 1178
Size: 30 165 30 601 30 871 30 4025
Fragments arranged by size:
4025 871 601 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2305 2902 1186
Size: 1119 597 4066
Fragments arranged by size:
4066 1119 597
BseYI C'CCAG_C
Cuts at: 2063 2359 2698 4985 5481 2063
Size: 296 339 2287 496 2364
Fragments arranged by size:
2364 2287 496 339 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2420 2759 3843 3928 4999 2420
Size: 339 1084 85 1071 3203
Fragments arranged by size:
3203 1084 1071 339 85
BsmI GAATG_Cn'
Cuts at: 2311 3293 3386 2311
Size: 982 93 4707
Fragments arranged by size:
4707 982 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2420 2759 3101 3843 3928 4999 1391
Size: 399 630 339 342 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 630 399 342 339 85
BspEI T'CCGG_A
Cuts at: 2465 5445 2465
Size: 2980 2802
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2481 2530 3137 844
Size: 1637 49 607 3489
Fragments arranged by size:
3489 1637 607 49
BsrBI CCG'CTC
Cuts at: 1394 1823 3669 1394
Size: 429 1846 3507
Fragments arranged by size:
3507 1846 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2044 4277 4451 65
Size: 1183 796 2233 174 1396
Fragments arranged by size:
2233 1396 1183 796 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 4426 4613 1700
Size: 110 286 2330 187 2869
Fragments arranged by size:
2869 2330 286 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2767 2767
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2582 2936 3112 3479 3864 3881 5333 5419
Size: 610 354 176 367 385 17 1452 86
Cuts at: 5419 5431 5777 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 610 385 367 354 346 176
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5043
Fragments arranged by size:
5043 683 56
BtsI GCAGTG_nn'
Cuts at: 3298 4092 4112 3298
Size: 794 20 4968
Fragments arranged by size:
4968 794 20
ClaI AT'CG_AT
Cuts at: 1554 2719 1554
Size: 1165 4617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 2547 3086 2547
Size: 539 5243
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2797 4789 809
Size: 507 1481 1992 1802
Fragments arranged by size:
1992 1802 1481 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747
Size: 54 1425 50 275 18 1399 889 1629
Cuts at: 5747 8
Size: 43
Fragments arranged by size:
1629 1425 1399 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2990 3057 3712 1353
Size: 1637 67 655 3423
Fragments arranged by size:
3423 1637 655 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2066 4369 4743 4889 387
Size: 1064 138 477 2303 374 146 1280
Fragments arranged by size:
2303 1280 1064 477 374 146 138
EcoICRI GAG'CTC
Cuts at: 2757 2757
Size: 5782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2794 2809 3845 4442 5229 5497 1239
Size: 393 1162 15 1036 597 787 268 1524
Fragments arranged by size:
1524 1162 1036 787 597 393 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2691 2691
Size: 5782
EcoO109I rG'GnC_Cy
Cuts at: 2292 2982 2292
Size: 690 5092
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3043 3043
Size: 5782
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 4929 1678
Size: 3251 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2473 2505 2567 2599 2651
Size: 32 135 32 901 32 62 32 52
Cuts at: 2651 2683 4423 4455 4497 4529 1373
Size: 32 1740 32 42 32 2626
Fragments arranged by size:
2626 1740 901 135 62 52 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2449 3126 3372 669
Size: 988 281 511 677 246 3079
Fragments arranged by size:
3079 988 677 511 281 246
HindIII A'AGCT_T
Cuts at: 748 2231 748
Size: 1483 4299
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2619 2765 3740 4099 4258 518
Size: 648 1453 146 975 359 159 2042
Fragments arranged by size:
2042 1453 975 648 359 159 146
MspA1I CmG'CkG
Cuts at: 1606 2739 2934 3198 3875 5023 5268 1606
Size: 1133 195 264 677 1148 245 2120
Fragments arranged by size:
2120 1148 1133 677 264 245 195
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5230
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 2137 1052
Size: 1085 4697
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2977 3140 4685 1132
Size: 97 1748 163 1545 2229
Fragments arranged by size:
2229 1748 1545 163 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2982 2982
Size: 5782
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2541 2592 3134 830
Size: 1711 51 542 3478
Fragments arranged by size:
3478 1711 542 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2739 2739
Size: 5782
SacI G_AGCT'C
Cuts at: 2759 2759
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SanDI GG'GwC_CC
Cuts at: 2982 2982
Size: 5782
SapI GCTCTTCn'nnn_
Cuts at: 2990 2990
Size: 5782
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2537 2588 3130 4265 4946 5137 826
Size: 219 1492 51 542 1135 681 191 1471
Fragments arranged by size:
1492 1471 1135 681 542 219 191 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2865 3221 513
Size: 552 1800 356 3074
Fragments arranged by size:
3074 1800 552 356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2560 3809 3826 3979 4164 4609 325
Size: 1255 980 1249 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1249 980 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 2840 4028
Size: 274 80 33 51 1443 600 263 1188
Cuts at: 4028 5740 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1188 600 274 263 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI