Restriction map for
pFN21AE0600
(Circular) (Six-base) MAPSORT of: pFN21AE0600.seq Check: 253 from: 1 to: 20605
pFN21AE0600 20605 bp
With 182 enzymes: SgfI *
May 13, 2010 16:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3457 7420 8209 11608 16723 3457
Size: 3963 789 3399 5115 7339
Fragments arranged by size:
7339 5115 3963 3399 789
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 18415 278
Size: 53 83 186 936 16879 2468
Fragments arranged by size:
16879 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2727 5597 6320 8422 8917 10953 11305 11943
Size: 790 2870 723 2102 495 2036 352 638
Cuts at: 11943 12446 13512 17948 1937
Size: 503 1066 4436 4594
Fragments arranged by size:
4594 4436 2870 2102 2036 1066 790 723
638 503 495 352
Acc65I G'GTAC_C
Cuts at: 17926 17926
Size: 20605
AclI AA'CG_TT
Cuts at: 1563 2252 18732 19105 1563
Size: 689 16480 373 3063
Fragments arranged by size:
16480 3063 689 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3524 3765 4025 4363 4967 6120 6471 6575
Size: 1892 241 260 338 604 1153 351 104
Cuts at: 6575 6584 7068 7427 7445 8862 8930 9104 10064
Size: 9 484 359 18 1417 68 174 960
Cuts at: 10064 10341 10656 11313 11585 12857 12919 13527 13737
Size: 277 315 657 272 1272 62 608 210
Cuts at: 13737 14004 14090 14705 15491 15923 16119 16707 17766
Size: 267 86 615 786 432 196 588 1059
Cuts at: 17766 18668 20052 20320 1632
Size: 902 1384 268 1917
Fragments arranged by size:
1917 1892 1417 1384 1272 1153 1059 960
902 786 657 615 608 604 588 484
432 359 351 338 315 277 272 268
267 260 241 210 196 174 104 86
68 62 18 9
AfeI AGC'GCT
Cuts at: 5735 17794 5735
Size: 12059 8546
AflII C'TTAA_G
Cuts at: 820 1017 6166 14644 820
Size: 197 5149 8478 6781
Fragments arranged by size:
8478 6781 5149 197
AflIII A'CryG_T
Cuts at: 1203 6283 6953 6955 9054 9573 12962 13341 16925
Size: 5080 670 2 2099 519 3389 379 3584
Cuts at: 16925 19504 20481 1203
Size: 2579 977 1327
Fragments arranged by size:
5080 3584 3389 2579 2099 1327 977 670
519 379 2
AgeI A'CCGG_T
Cuts at: 3400 17187 19436 3400
Size: 13787 2249 4569
Fragments arranged by size:
13787 4569 2249
AhdI GACnn_n'nnGTC
Cuts at: 2514 2801 7646 13905 14959 17892 19334 2514
Size: 287 4845 6259 1054 2933 1442 3785
Fragments arranged by size:
6259 4845 3785 2933 1442 1054 287
AleI CACnn'nnGTG
Cuts at: 1166 2870 12945 14268 1166
Size: 1704 10075 1323 7503
Fragments arranged by size:
10075 7503 1704 1323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2390 3390 3854 5280 6555 8304 8791 10228
Size: 1206 1000 464 1426 1275 1749 487 1437
Cuts at: 10228 14951 1184
Size: 4723 6838
Fragments arranged by size:
6838 4723 1749 1437 1426 1275 1206 1000
487 464
AlwNI CAG_nnn'CTG
Cuts at: 3484 4048 5042 5098 6412 6670 7111 7357 7369
Size: 564 994 56 1314 258 441 246 12
Cuts at: 7369 7889 9011 9181 9683 9826 9908 9991 10981
Size: 520 1122 170 502 143 82 83 990
Cuts at: 10981 12182 13122 15901 15976 18348 19920 3484
Size: 1201 940 2779 75 2372 1572 4169
Fragments arranged by size:
4169 2779 2372 1572 1314 1201 1122 994
990 940 564 520 502 441 258 246
170 143 83 82 75 56 12
ApaI G_GGCC'C
Cuts at: 9555 13075 9555
Size: 3520 17085
ApaLI G'TGCA_C
Cuts at: 15809 18662 19818 15809
Size: 2853 1156 16596
Fragments arranged by size:
16596 2853 1156
ApoI r'AATT_y
Cuts at: 1493 3093 3161 3949 4055 5214 5635 6017 7276
Size: 1600 68 788 106 1159 421 382 1259
Cuts at: 7276 7460 8795 9298 9309 9387 9617 9939 11259
Size: 184 1335 503 11 78 230 322 1320
Cuts at: 11259 13733 15077 15085 15098 15307 16181 16805 17042
Size: 2474 1344 8 13 209 874 624 237
Cuts at: 17042 17279 17342 17914 18140 18292 20401 1493
Size: 237 63 572 226 152 2109 1697
Fragments arranged by size:
2474 2109 1697 1600 1344 1335 1320 1259
1159 874 788 624 572 503 421 382
322 237 237 230 226 209 184 152
106 78 68 63 13 11 8
AseI AT'TA_AT
Cuts at: 160 1032 11642 19159 160
Size: 872 10610 7517 1606
Fragments arranged by size:
10610 7517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 20605
AvaI C'yCGr_G
Cuts at: 1870 1943 2379 3714 5792 7141 7547 13137 13710
Size: 73 436 1335 2078 1349 406 5590 573
Cuts at: 13710 16872 17418 17930 1870
Size: 3162 546 512 4545
Fragments arranged by size:
5590 4545 3162 2078 1349 1335 573 546
512 436 406 73
AvrII C'CTAG_G
Cuts at: 15643 15643
Size: 20605
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 12524 12557 12524
Size: 33 20572
BamHI G'GATC_C
Cuts at: 10555 11593 13935 16419 17935 18302 10555
Size: 1038 2342 2484 1516 367 12858
Fragments arranged by size:
12858 2484 2342 1516 1038 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4035 4747 5772 12637 17630
Size: 325 217 627 2248 712 1025 6865 4993
Cuts at: 17630 17926 19381 618
Size: 296 1455 1842
Fragments arranged by size:
6865 4993 2248 1842 1455 1025 712 627
325 296 217
BanII G_rGCy'C
Cuts at: 1391 3905 6881 8447 8595 8663 9555 10395 10918
Size: 2514 2976 1566 148 68 892 840 523
Cuts at: 10918 11838 13075 13116 13381 14404 14529 16312 16398
Size: 920 1237 41 265 1023 125 1783 86
Cuts at: 16398 17924 1391
Size: 1526 4072
Fragments arranged by size:
4072 2976 2514 1783 1566 1526 1237 1023
920 892 840 523 265 148 125 86
68 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 2868 3530 4485 5004 8616 8747
Size: 971 102 867 662 955 519 3612 131
Cuts at: 8747 10095 10500 12093 15542 17870 928
Size: 1348 405 1593 3449 2328 3663
Fragments arranged by size:
3663 3612 3449 2328 1593 1348 971 955
867 662 519 405 131 102
BbvCI CC'TCA_GC
Cuts at: 4945 11034 12473 15795 4945
Size: 6089 1439 3322 9755
Fragments arranged by size:
9755 6089 3322 1439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4997 5031 5681 5715 12203 12237 16097
Size: 34 3060 34 650 34 6488 34 3860
Cuts at: 16097 16131 18796 18830 1903
Size: 34 2665 34 3678
Fragments arranged by size:
6488 3860 3678 3060 2665 650 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5831 15661 18497 19707 5831
Size: 9830 2836 1210 6729
Fragments arranged by size:
9830 6729 2836 1210
BclI T'GATC_A
Cuts at: 1548 4719 10662 13174 15300 1548
Size: 3171 5943 2512 2126 6853
Fragments arranged by size:
6853 5943 3171 2512 2126
BfrBI ATG'CAT
Cuts at: 5267 7528 8768 5267
Size: 2261 1240 17104
Fragments arranged by size:
17104 2261 1240
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6726 9281 19216
Size: 107 122 71 631 605 5054 2555 9935
Cuts at: 19216 136
Size: 1525
Fragments arranged by size:
9935 5054 2555 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2317 2547 5569 6471 9031 10857 20600 2317
Size: 230 3022 902 2560 1826 9743 2322
Fragments arranged by size:
9743 3022 2560 2322 1826 902 230
BlpI GC'TnA_GC
Cuts at: 5951 6875 7959 8106 11810 12126 12867 13115 16338
Size: 924 1084 147 3704 316 741 248 3223
Cuts at: 16338 16392 16503 18022 5951
Size: 54 111 1519 8534
Fragments arranged by size:
8534 3704 3223 1519 1084 924 741 316
248 147 111 54
Bme1580I G_kGCm'C
Cuts at: 1790 9555 12642 13075 13144 15813 18666 19822 1790
Size: 7765 3087 433 69 2669 2853 1156 2573
Fragments arranged by size:
7765 3087 2853 2669 2573 1156 433 69
BmgBI CAC'GTC
Cuts at: 1318 11266 16549 16928 1318
Size: 9948 5283 379 4995
Fragments arranged by size:
9948 5283 4995 379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2807 4297 7288
Size: 416 53 83 399 24 1390 1490 2991
Cuts at: 7288 8938 9808 11971 12532 13399 15264 15881 18336
Size: 1650 870 2163 561 867 1865 617 2455
Cuts at: 18336 19294 442
Size: 958 1753
Fragments arranged by size:
2991 2455 2163 1865 1753 1650 1490 1390
958 870 867 617 561 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 7152 1056
Size: 6096 14509
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2124 2377 3919 7156 9181 11167 12639 2124
Size: 253 1542 3237 2025 1986 1472 10090
Fragments arranged by size:
10090 3237 2025 1986 1542 1472 253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2692 2829 2929 3461 3833 4007 4147 4211
Size: 1453 137 100 532 372 174 140 64
Cuts at: 4211 4418 4472 5204 5603 6263 6692 6926 6995
Size: 207 54 732 399 660 429 234 69
Cuts at: 6995 7538 7898 7926 8186 9705 9710 9815 10103
Size: 543 360 28 260 1519 5 105 288
Cuts at: 10103 10374 10922 11129 11204 11498 11894 12041 12664
Size: 271 548 207 75 294 396 147 623
Cuts at: 12664 13149 13481 13943 14646 15953 16772 16811 16852
Size: 485 332 462 703 1307 819 39 41
Cuts at: 16852 17431 17478 17800 17874 19265 1239
Size: 579 47 322 74 1391 2579
Fragments arranged by size:
2579 1519 1453 1391 1307 819 732 703
660 623 579 548 543 532 485 462
429 399 396 372 360 332 322 294
288 271 260 234 207 207 174 147
140 137 105 100 75 74 69 64
54 47 41 39 28 5
Bpu10I CC'TnA_GC
Cuts at: 4945 6921 7302 8257 9663 10790 11034 12473 13187
Size: 1976 381 955 1406 1127 244 1439 714
Cuts at: 13187 15275 15795 4945
Size: 2088 520 9755
Fragments arranged by size:
9755 2088 1976 1439 1406 1127 955 714
520 381 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3168 3889 4268 4429 6135 7639 8657 10016 10433
Size: 721 379 161 1706 1504 1018 1359 417
Cuts at: 10433 10863 10874 14084 16052 18730 19595 19893 3168
Size: 430 11 3210 1968 2678 865 298 3880
Fragments arranged by size:
3880 3210 2678 1968 1706 1504 1359 1018
865 721 430 417 379 298 161 11
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3455 5450 6356 7544 9923 11351 14482
Size: 617 1956 1995 906 1188 2379 1428 3131
Cuts at: 14482 14496 15222 16427 19268 882
Size: 14 726 1205 2841 2219
Fragments arranged by size:
3131 2841 2379 2219 1995 1956 1428 1205
1188 906 726 617 14
BsaAI yAC'GTr
Cuts at: 493 1204 5495 6211 6884 6956 18396 493
Size: 711 4291 716 673 72 11440 2702
Fragments arranged by size:
11440 4291 2702 716 711 673 72
BsaBI GATnn'nnATC
Cuts at: 7774 16298 17076 20599 7774
Size: 8524 778 3523 7780
Fragments arranged by size:
8524 7780 3523 778
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6238 18412 18794 19429
Size: 53 83 186 936 4705 12174 382 635
Cuts at: 19429 275
Size: 1451
Fragments arranged by size:
12174 4705 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3400 3585 4026 8799 17187 19038 19436 19710 19857
Size: 185 441 4773 8388 1851 398 274 147
Cuts at: 19857 20268 3400
Size: 411 3737
Fragments arranged by size:
8388 4773 3737 1851 441 411 398 274
185 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2648 2678 3908 3938 3980
Size: 30 165 30 1245 30 1230 30 42
Cuts at: 3980 4010 8159 8189 9032 9062 10283 10313 14672
Size: 30 4149 30 843 30 1221 30 4359
Cuts at: 14672 14702 14990 15020 16645 16675 1178
Size: 30 288 30 1625 30 5108
Fragments arranged by size:
5108 4359 4149 1625 1245 1230 1221 843
288 165 42 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3730 4118 4121 4208 5078 5144 5201 6260
Size: 2544 388 3 87 870 66 57 1059
Cuts at: 6260 6263 6266 6284 7358 7868 9158 11513 11846
Size: 3 3 18 1074 510 1290 2355 333
Cuts at: 11846 12335 12500 12503 12986 13376 13622 14717 16334
Size: 489 165 3 483 390 246 1095 1617
Cuts at: 16334 16538 16650 16808 1186
Size: 204 112 158 4983
Fragments arranged by size:
4983 2544 2355 1617 1290 1095 1074 1059
870 510 489 483 390 388 333 246
204 165 158 112 87 66 57 18
3 3 3 3
BseYI C'CCAG_C
Cuts at: 3898 4258 5754 5787 5892 6024 6312 6738 7266
Size: 360 1496 33 105 132 288 426 528
Cuts at: 7266 7369 9501 9547 10140 10709 11043 14676 14880
Size: 103 2132 46 593 569 334 3633 204
Cuts at: 14880 15987 17503 17687 19808 20304 3898
Size: 1107 1516 184 2121 496 4199
Fragments arranged by size:
4199 3633 2132 2121 1516 1496 1107 593
569 528 496 426 360 334 288 204
184 132 105 103 46 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 7454 10583 11673 14108 14134 15344
Size: 573 705 5013 3129 1090 2435 26 1210
Cuts at: 15344 1163
Size: 6424
Fragments arranged by size:
6424 5013 3129 2435 1210 1090 705 573
26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2096 3266 9512 10105 17011 18055
Size: 275 186 95 1170 6246 593 6906 1044
Cuts at: 18055 18816 19844 1540
Size: 761 1028 2301
Fragments arranged by size:
6906 6246 2301 1170 1044 1028 761 593
275 186 95
BsiHKAI G_wGCw'C
Cuts at: 5292 6712 7965 8447 8595 8643 8663 9812 10014
Size: 1420 1253 482 148 48 20 1149 202
Cuts at: 10014 10395 10918 11838 12585 12625 13116 14529 15195
Size: 381 523 920 747 40 491 1413 666
Cuts at: 15195 15592 15813 15983 16132 16312 16398 17311 18666
Size: 397 221 170 149 180 86 913 1355
Cuts at: 18666 18751 19822 5292
Size: 85 1071 6075
Fragments arranged by size:
6075 1420 1413 1355 1253 1149 1071 920
913 747 666 523 491 482 397 381
221 202 180 170 149 148 86 85
48 40 20
BsmI GAATG_Cn'
Cuts at: 6544 9586 18116 18209 6544
Size: 3042 8530 93 8940
Fragments arranged by size:
8940 8530 3042 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2018 14294 17750 2018
Size: 12276 3456 4873
Fragments arranged by size:
12276 4873 3456
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3905 5292 6712 6881 7965 8447 8595
Size: 399 2115 1387 1420 169 1084 482 148
Cuts at: 8595 8643 8663 9555 9812 10014 10395 10918 11838
Size: 48 20 892 257 202 381 523 920
Cuts at: 11838 12585 12625 12642 13075 13116 13144 13381 14404
Size: 747 40 17 433 41 28 237 1023
Cuts at: 14404 14529 15195 15592 15813 15983 16132 16312 16398
Size: 125 666 397 221 170 149 180 86
Cuts at: 16398 17311 17924 18666 18751 19822 1391
Size: 913 613 742 85 1071 2174
Fragments arranged by size:
2174 2115 1420 1387 1084 1071 1023 920
913 892 747 742 666 613 523 482
433 399 397 381 257 237 221 202
180 170 169 149 148 125 86 85
48 41 40 28 20 17
BspEI T'CCGG_A
Cuts at: 4026 8799 20268 4026
Size: 4773 11469 4363
Fragments arranged by size:
11469 4773 4363
BspHI T'CATG_A
Cuts at: 9100 13177 13429 15831 16185 18494 20224 9100
Size: 4077 252 2402 354 2309 1730 9481
Fragments arranged by size:
9481 4077 2402 2309 1730 354 252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 3457 3846 4491 6397 7420 8209 11608
Size: 1307 1306 389 645 1906 1023 789 3399
Cuts at: 11608 16723 17960 844
Size: 5115 1237 3489
Fragments arranged by size:
5115 3489 3399 1906 1307 1306 1237 1023
789 645 389
BsrBI CCG'CTC
Cuts at: 1394 1823 2038 2056 2092 10237 10623 13069 15694
Size: 429 215 18 36 8145 386 2446 2625
Cuts at: 15694 16756 17388 18492 1394
Size: 1062 632 1104 3507
Fragments arranged by size:
8145 3507 2625 2446 1104 1062 632 429
386 215 36 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 2482 3838 4749 5781 7232 8286 11804
Size: 1183 1234 1356 911 1032 1451 1054 3518
Cuts at: 11804 14608 19100 19274 65
Size: 2804 4492 174 1396
Fragments arranged by size:
4492 3518 2804 1451 1396 1356 1234 1183
1054 1032 911 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3265 3400 16026 17187 17718 19249 19436
Size: 110 1455 135 12626 1161 531 1531 187
Cuts at: 19436 1700
Size: 2869
Fragments arranged by size:
12626 2869 1531 1455 1161 531 187 135
110
BsrGI T'GTAC_A
Cuts at: 96 3429 3572 4912 5148 7763 11671 17474 96
Size: 3333 143 1340 236 2615 3908 5803 3227
Fragments arranged by size:
5803 3908 3333 3227 2615 1340 236 143
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 20605
BssSI C'ACGA_G
Cuts at: 4389 7998 9968 17310 18665 19677 4389
Size: 3609 1970 7342 1355 1012 5317
Fragments arranged by size:
7342 5317 3609 1970 1355 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2204 2419 4852 7354 10130 794
Size: 1410 215 2433 2502 2776 11269
Fragments arranged by size:
11269 2776 2502 2433 1410 215
BstBI TT'CG_AA
Cuts at: 8409 19409 8409
Size: 11000 9605
BstEII G'GTnAC_C
Cuts at: 1184 3553 8329 16558 1184
Size: 2369 4776 8229 5231
Fragments arranged by size:
8229 5231 4776 2369
BstXI CCAn_nnnn'nTGG
Cuts at: 4798 5441 5683 4798
Size: 643 242 19720
Fragments arranged by size:
19720 643 242
BstYI r'GATC_y
Cuts at: 1972 2317 2547 3472 3480 5569 6471 6676 6745
Size: 345 230 925 8 2089 902 205 69
Cuts at: 6745 7775 8583 9031 9042 10555 10857 11003 11593
Size: 1030 808 448 11 1513 302 146 590
Cuts at: 11593 11889 13642 13935 16419 17935 18302 18687 18704
Size: 296 1753 293 2484 1516 367 385 17
Cuts at: 18704 20156 20242 20254 20600 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2484 2089 1977 1753 1516 1513 1452 1030
925 902 808 590 448 385 367 346
345 302 296 293 230 205 146 86
69 17 12 11 8
Bsu36I CC'TnA_GG
Cuts at: 2907 7341 10947 13296 2907
Size: 4434 3606 2349 10216
Fragments arranged by size:
10216 4434 3606 2349
BtgI C'CryG_G
Cuts at: 513 1065 4497 5109 5854 9210 9512 10505 13554
Size: 552 3432 612 745 3356 302 993 3049
Cuts at: 13554 13600 16233 16700 513
Size: 46 2633 467 4418
Fragments arranged by size:
4418 3432 3356 3049 2633 993 745 612
552 467 302 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 10011 487
Size: 683 56 8785 11081
Fragments arranged by size:
11081 8785 683 56
BtsI GCAGTG_nn'
Cuts at: 2147 3362 4316 5886 6990 7743 8376 9182 11128
Size: 1215 954 1570 1104 753 633 806 1946
Cuts at: 11128 17871 18121 18915 18935 2147
Size: 6743 250 794 20 3817
Fragments arranged by size:
6743 3817 1946 1570 1215 1104 954 806
794 753 633 250 20
ClaI AT'CG_AT
Cuts at: 1554 7059 16941 1554
Size: 5505 9882 5218
Fragments arranged by size:
9882 5505 5218
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6802 6837 6870 6905 11362 11397 16250
Size: 35 6238 35 33 35 4457 35 4853
Cuts at: 16250 16285 17109 17144 529
Size: 35 824 35 3990
Fragments arranged by size:
6238 4853 4457 3990 824 35 35 35
35 35 35 33
DraI TTT'AAA
Cuts at: 11569 12237 15135 17909 11569
Size: 668 2898 2774 14265
Fragments arranged by size:
14265 2898 2774 668
DraIII CAC_nnn'GTG
Cuts at: 2262 2262
Size: 20605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 14510 16826 19612 809
Size: 507 13194 2316 2786 1802
Fragments arranged by size:
13194 2786 2316 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2329 3099 3166
Size: 54 1425 50 275 18 499 770 67
Cuts at: 3166 3263 3729 4800 5139 6480 7287 7324 9509
Size: 97 466 1071 339 1341 807 37 2185
Cuts at: 9509 11119 11632 12102 12429 18052 18941 20570 8
Size: 1610 513 470 327 5623 889 1629 43
Fragments arranged by size:
5623 2185 1629 1610 1425 1341 1071 889
807 770 513 499 470 466 339 327
275 97 67 54 50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 3263 9509 18052 1537
Size: 275 1451 6246 8543 4090
Fragments arranged by size:
8543 6246 4090 1451 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2016 3207 3507 3789 3888 5055 6139 6261
Size: 663 1191 300 282 99 1167 1084 122
Cuts at: 6261 6414 6852 6903 6942 8906 9921 10260 10353
Size: 153 438 51 39 1964 1015 339 93
Cuts at: 10353 10404 10737 11316 11604 11691 12303 12938 13104
Size: 51 333 579 288 87 612 635 166
Cuts at: 13104 13890 13911 13988 14109 14463 14892 15048 15183
Size: 786 21 77 121 354 429 156 135
Cuts at: 15183 17061 18535 1353
Size: 1878 1474 3423
Fragments arranged by size:
3423 1964 1878 1474 1191 1167 1084 1015
786 663 635 612 579 438 429 354
339 333 300 288 282 166 156 153
135 122 121 99 93 87 77 51
51 39 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6982 19192 19566 19712 387
Size: 1064 138 5393 12210 374 146 1280
Fragments arranged by size:
12210 5393 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 8445 8593 8661 10393 10916 11836 13114 14527 16310
Size: 148 68 1732 523 920 1278 1413 1783
Cuts at: 16310 16396 8445
Size: 86 12654
Fragments arranged by size:
12654 1783 1732 1413 1278 920 523 148
86 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2692 2829 2929 3461 3524 3765 3833
Size: 393 1060 137 100 532 63 241 68
Cuts at: 3833 4007 4025 4147 4211 4363 4418 4472 4967
Size: 174 18 122 64 152 55 54 495
Cuts at: 4967 5204 5603 6120 6263 6471 6575 6584 6692
Size: 237 399 517 143 208 104 9 108
Cuts at: 6692 6926 6995 7068 7427 7445 7538 7898 7926
Size: 234 69 73 359 18 93 360 28
Cuts at: 7926 8186 8862 8930 9104 9705 9710 9815 10064
Size: 260 676 68 174 601 5 105 249
Cuts at: 10064 10103 10341 10374 10656 10922 11129 11204 11313
Size: 39 238 33 282 266 207 75 109
Cuts at: 11313 11498 11585 11894 12041 12664 12857 12919 13149
Size: 185 87 309 147 623 193 62 230
Cuts at: 13149 13481 13527 13737 13943 14004 14090 14646 14705
Size: 332 46 210 206 61 86 556 59
Cuts at: 14705 15491 15923 15953 16119 16707 16772 16811 16852
Size: 786 432 30 166 588 65 39 41
Cuts at: 16852 17431 17478 17766 17800 17874 18668 19265 20052
Size: 579 47 288 34 74 794 597 787
Cuts at: 20052 20320 1239
Size: 268 1524
Fragments arranged by size:
1524 1060 794 787 786 676 623 601
597 588 579 556 532 517 495 432
399 393 360 359 332 309 288 282
268 266 260 249 241 238 237 234
230 210 208 207 206 193 185 174
174 166 152 147 143 137 122 109
108 105 104 100 93 87 86 75
74 73 69 68 68 65 64 63
62 61 59 55 54 47 46 41
39 39 34 33 30 28 18 18
9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 4487 5657 6548 10077 12848 13299 17354 17881 4487
Size: 1170 891 3529 2771 451 4055 527 7211
Fragments arranged by size:
7211 4055 3529 2771 1170 891 527 451
EcoO109I rG'GnC_Cy
Cuts at: 2356 8814 9551 9552 9680 10173 10936 11509 12397
Size: 6458 737 1 128 493 763 573 888
Cuts at: 12397 15058 15817 15898 16150 16324 17894 2356
Size: 2661 759 81 252 174 1570 5067
Fragments arranged by size:
6458 5067 2661 1570 888 763 759 737
573 493 252 174 128 81 1
EcoRI G'AATT_C
Cuts at: 5214 5635 8795 9298 15077 15085 16181 17914 18292
Size: 421 3160 503 5779 8 1096 1733 378
Cuts at: 18292 5214
Size: 7527
Fragments arranged by size:
7527 5779 3160 1733 1096 503 421 378
8
EcoRV GAT'ATC
Cuts at: 15480 15480
Size: 20605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 8323 17751 8323
Size: 9428 11177
FspI TGC'GCA
Cuts at: 19111 19111
Size: 20605
HaeII r_GCGC'y
Cuts at: 1678 5737 6118 6941 10621 13600 17796 19752 1678
Size: 4059 381 823 3680 2979 4196 1956 2531
Fragments arranged by size:
4196 4059 3680 2979 2531 1956 823 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2500 2532 2787 2819 2825
Size: 32 135 32 928 32 255 32 6
Cuts at: 2825 2840 2857 2872 2907 2939 4385 4417 5686
Size: 15 17 15 35 32 1446 32 1269
Cuts at: 5686 5718 7048 7080 7632 7664 8756 8788 9206
Size: 32 1330 32 552 32 1092 32 418
Cuts at: 9206 9238 9721 9753 9989 10021 10424 10456 11137
Size: 32 483 32 236 32 403 32 681
Cuts at: 11137 11169 11692 11724 11991 12023 12131 12163 12850
Size: 32 523 32 267 32 108 32 687
Cuts at: 12850 12882 12991 13023 13515 13547 13891 13923 14893
Size: 32 109 32 492 32 344 32 970
Cuts at: 14893 14925 14945 14977 15219 15251 16056 16088 16205
Size: 32 20 32 242 32 805 32 117
Cuts at: 16205 16230 16237 16262 16912 16944 17348 17380 17747
Size: 25 7 25 650 32 404 32 367
Cuts at: 17747 17779 17878 17910 19246 19278 19320 19352 1373
Size: 32 99 32 1336 32 42 32 2626
Fragments arranged by size:
2626 1446 1336 1330 1269 1092 970 928
805 687 681 650 552 523 492 483
418 404 403 367 344 267 255 242
236 135 117 109 108 99 42 35
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
25 25 20 17 15 15 7 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2269 2508 4179 4861 5598 6891
Size: 988 281 331 239 1671 682 737 1293
Cuts at: 6891 11306 14590 14727 15763 16219 17949 18195 669
Size: 4415 3284 137 1036 456 1730 246 3079
Fragments arranged by size:
4415 3284 3079 1730 1671 1293 1036 988
737 682 456 331 281 246 239 137
HindIII A'AGCT_T
Cuts at: 748 4934 5073 6908 7582 8456 10807 11763 12164
Size: 4186 139 1835 674 874 2351 956 401
Cuts at: 12164 14255 15414 748
Size: 2091 1159 5939
Fragments arranged by size:
5939 4186 2351 2091 1835 1159 956 874
674 401 139
HpaI GTT'AAC
Cuts at: 4861 16219 18195 4861
Size: 11358 1976 7271
Fragments arranged by size:
11358 7271 1976
KpnI G_GTAC'C
Cuts at: 17930 17930
Size: 20605
MfeI C'AATT_G
Cuts at: 5312 6533 8133 8962 9920 11156 18204 5312
Size: 1221 1600 829 958 1236 7048 7713
Fragments arranged by size:
7713 7048 1600 1236 1221 958 829
MluI A'CGCG_T
Cuts at: 20481 20481
Size: 20605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2027 2045 2331 7476 8507 8602 8769 9254
Size: 722 18 286 5145 1031 95 167 485
Cuts at: 9254 9270 9841 10161 12576 13431 15687 16065 16832
Size: 16 571 320 2415 855 2256 378 767
Cuts at: 16832 17558 17799 19719 19903 1305
Size: 726 241 1920 184 2007
Fragments arranged by size:
5145 2415 2256 2007 1920 1031 855 767
726 722 571 485 378 320 286 241
184 167 95 18 16
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2331 3101 3731 4802 5141
Size: 54 1425 343 499 770 630 1071 339
Cuts at: 5141 6482 7289 7326 11121 11634 12104 12431 10
Size: 1341 807 37 3795 513 470 327 8184
Fragments arranged by size:
8184 3795 1425 1341 1071 807 770 630
513 499 470 343 339 327 54 37
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 2870 3198 3236 3885 4796 5439
Size: 648 1135 569 328 38 649 911 643
Cuts at: 5439 7193 8043 8267 12945 14268 17240 18563 18922
Size: 1754 850 224 4678 1323 2972 1323 359
Cuts at: 18922 19081 518
Size: 159 2042
Fragments arranged by size:
4678 2972 2042 1754 1323 1323 1135 911
850 649 648 643 569 359 328 224
159 38
MspA1I CmG'CkG
Cuts at: 1606 2630 3463 4076 4324 4702 4852 5758 7108
Size: 1024 833 613 248 378 150 906 1350
Cuts at: 7108 8404 11422 11814 11914 12019 12082 12125 12427
Size: 1296 3018 392 100 105 63 43 302
Cuts at: 12427 14884 15790 16450 16626 16725 17472 18021 18698
Size: 2457 906 660 176 99 747 549 677
Cuts at: 18698 19846 20091 1606
Size: 1148 245 2120
Fragments arranged by size:
3018 2457 2120 1350 1296 1148 1024 906
906 833 747 677 660 613 549 392
378 302 248 245 176 150 105 100
99 63 43
NaeI GCC'GGC
Cuts at: 3267 16028 17720 3267
Size: 12761 1692 6152
Fragments arranged by size:
12761 6152 1692
NcoI C'CATG_G
Cuts at: 513 1065 4497 5109 5854 9210 10505 16233 513
Size: 552 3432 612 745 3356 1295 5728 4885
Fragments arranged by size:
5728 4885 3432 3356 1295 745 612 552
NdeI CA'TA_TG
Cuts at: 387 4120 11310 14865 387
Size: 3733 7190 3555 6127
Fragments arranged by size:
7190 6127 3733 3555
NgoMIV G'CCGG_C
Cuts at: 3265 16026 17718 3265
Size: 12761 1692 6152
Fragments arranged by size:
12761 6152 1692
NheI G'CTAG_C
Cuts at: 1052 7148 1052
Size: 6096 14509
NotI GC'GGCC_GC
Cuts at: 18052 18052
Size: 20605
NsiI A_TGCA'T
Cuts at: 5269 7530 8770 5269
Size: 2261 1240 17104
Fragments arranged by size:
17104 2261 1240
NspI r_CATG'y
Cuts at: 1132 1229 4349 4859 6127 6287 7528 8048 9058
Size: 97 3120 510 1268 160 1241 520 1010
Cuts at: 9058 9577 10490 11950 12966 13345 14432 15014 16081
Size: 519 913 1460 1016 379 1087 582 1067
Cuts at: 16081 17963 19508 1132
Size: 1882 1545 2229
Fragments arranged by size:
3120 2229 1882 1545 1460 1268 1241 1087
1067 1016 1010 913 582 520 519 510
379 160 97
PasI CC'CwG_GG
Cuts at: 1348 2120 2512 14743 17368 17675 1348
Size: 772 392 12231 2625 307 4278
Fragments arranged by size:
12231 4278 2625 772 392 307
PciI A'CATG_T
Cuts at: 6283 9054 9573 12962 13341 19504 6283
Size: 2771 519 3389 379 6163 7384
Fragments arranged by size:
7384 6163 3389 2771 519 379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6670 10726 13828 14498 17510 1108
Size: 5562 4056 3102 670 3012 4203
Fragments arranged by size:
5562 4203 4056 3102 3012 670
PfoI T'CCnGG_A
Cuts at: 4488 5652 5823 6942 7068 7123 7914 8154 8247
Size: 1164 171 1119 126 55 791 240 93
Cuts at: 8247 8586 9831 10351 10695 10800 12336 13662 15969
Size: 339 1245 520 344 105 1536 1326 2307
Cuts at: 15969 16398 17816 4488
Size: 429 1418 7277
Fragments arranged by size:
7277 2307 1536 1418 1326 1245 1164 1119
791 520 429 344 339 240 171 126
105 93 55
PmeI GTTT'AAAC
Cuts at: 17909 17909
Size: 20605
PmlI CAC'GTG
Cuts at: 6211 6884 6956 6211
Size: 673 72 19860
Fragments arranged by size:
19860 673 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 18674 18706 20187 20219 18674
Size: 32 1481 32 19060
Fragments arranged by size:
19060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2356 8814 10173 15817 15898 16324 17894 2356
Size: 6458 1359 5644 81 426 1570 5067
Fragments arranged by size:
6458 5644 5067 1570 1359 426 81
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 20605
PsiI TTA'TAA
Cuts at: 3063 14907 18175 3063
Size: 11844 3268 5493
Fragments arranged by size:
11844 5493 3268
PspOMI G'GGCC_C
Cuts at: 9551 13071 9551
Size: 3520 17085
PstI C_TGCA'G
Cuts at: 830 2200 2415 3369 3468 5010 5763 6642 7470
Size: 1370 215 954 99 1542 753 879 828
Cuts at: 7470 7839 8127 8379 8505 9177 9496 10432 10752
Size: 369 288 252 126 672 319 936 320
Cuts at: 10752 10935 13575 14736 16158 17800 17957 830
Size: 183 2640 1161 1422 1642 157 3478
Fragments arranged by size:
3478 2640 1642 1542 1422 1370 1161 954
936 879 828 753 672 369 320 319
288 252 215 183 157 126 99
PvuI CG_AT'CG
Cuts at: 2001 10105 2001
Size: 8104 12501
PvuII CAG'CTG
Cuts at: 2630 3463 4076 4324 4852 5758 7108 11422 11814
Size: 833 613 248 528 906 1350 4314 392
Cuts at: 11814 12019 12082 12125 12427 14884 15790 17472 2630
Size: 205 63 43 302 2457 906 1682 5763
Fragments arranged by size:
5763 4314 2457 1682 1350 906 906 833
613 528 392 302 248 205 63 43
SacI G_AGCT'C
Cuts at: 8447 8595 8663 10395 10918 11838 13116 14529 16312
Size: 148 68 1732 523 920 1278 1413 1783
Cuts at: 16312 16398 8447
Size: 86 12654
Fragments arranged by size:
12654 1783 1732 1413 1278 920 523 148
86 68
SalI G'TCGA_C
Cuts at: 1936 5596 11304 17947 1936
Size: 3660 5708 6643 4594
Fragments arranged by size:
6643 5708 4594 3660
SapI GCTCTTCn'nnn_
Cuts at: 3507 3888 6414 6903 10404 10737 12303 13104 13911
Size: 381 2526 489 3501 333 1566 801 807
Cuts at: 13911 13988 15183 17061 3507
Size: 77 1195 1878 7051
Fragments arranged by size:
7051 3501 2526 1878 1566 1195 807 801
489 381 333 77
SbfI CC_TGCA'GG
Cuts at: 7839 17957 7839
Size: 10118 10487
ScaI AGT'ACT
Cuts at: 5562 6032 8359 9096 18853 20565 5562
Size: 470 2327 737 9757 1712 5602
Fragments arranged by size:
9757 5602 2327 1712 737 470
SexAI A'CCwGG_T
Cuts at: 8643 10175 8643
Size: 1532 19073
SfcI C'TryA_G
Cuts at: 826 1045 2196 2411 3293 3365 3464 4071 4639
Size: 219 1151 215 882 72 99 607 568
Cuts at: 4639 5006 5759 6638 7466 7835 8058 8095 8123
Size: 367 753 879 828 369 223 37 28
Cuts at: 8123 8361 8375 8423 8501 8735 9173 9492 10241
Size: 238 14 48 78 234 438 319 749
Cuts at: 10241 10253 10428 10748 10885 10931 11455 11756 13571
Size: 12 175 320 137 46 524 301 1815
Cuts at: 13571 14732 16154 17796 17953 19088 19769 19960 826
Size: 1161 1422 1642 157 1135 681 191 1471
Fragments arranged by size:
1815 1642 1471 1422 1161 1151 1135 882
879 828 753 749 681 607 568 524
438 369 367 320 319 301 238 234
223 219 215 191 175 157 137 99
78 72 48 46 37 28 14 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 20605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 20605
SmaI CCC'GGG
Cuts at: 1872 2381 17420 17932 1872
Size: 509 15039 512 4545
Fragments arranged by size:
15039 4545 512 509
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3183 3904 4247 4408 5792 6150
Size: 197 926 1240 721 343 161 1384 358
Cuts at: 6150 6166 7618 8636 10031 10448 10853 10878 14063
Size: 16 1452 1018 1395 417 405 25 3185
Cuts at: 14063 14644 16031 16872 18709 19610 19872 820
Size: 581 1387 841 1837 901 262 1553
Fragments arranged by size:
3185 1837 1553 1452 1395 1387 1384 1240
1018 926 901 841 721 581 417 405
358 343 262 197 161 25 16
SnaBI TAC'GTA
Cuts at: 493 5495 18396 493
Size: 5002 12901 2702
Fragments arranged by size:
12901 5002 2702
SpeI A'CTAG_T
Cuts at: 152 2461 12700 152
Size: 2309 10239 8057
Fragments arranged by size:
10239 8057 2309
SphI G_CATG'C
Cuts at: 1132 6127 14432 17963 1132
Size: 4995 8305 3531 3774
Fragments arranged by size:
8305 4995 3774 3531
SspI AAT'ATT
Cuts at: 5 52 2470 4442 10638 10819 18529 5
Size: 47 2418 1972 6196 181 7710 2081
Fragments arranged by size:
7710 6196 2418 2081 1972 181 47
StuI AGG'CCT
Cuts at: 1517 6080 7307 7841 9275 11288 13589 16040 1517
Size: 4563 1227 534 1434 2013 2301 2451 6082
Fragments arranged by size:
6082 4563 2451 2301 2013 1434 1227 534
StyI C'CwwG_G
Cuts at: 513 1065 2592 3102 3666 4497 5109 5670 5676
Size: 552 1527 510 564 831 612 561 6
Cuts at: 5676 5854 6724 7167 9019 9210 10078 10505 11796
Size: 178 870 443 1852 191 868 427 1291
Cuts at: 11796 12393 13939 14413 14892 15643 16233 16779 17271
Size: 597 1546 474 479 751 590 546 492
Cuts at: 17271 17517 17806 18044 513
Size: 246 289 238 3074
Fragments arranged by size:
3074 1852 1546 1527 1291 870 868 831
751 612 597 590 564 561 552 546
510 492 479 474 443 427 289 246
238 191 178 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4272 4792 5309 6304 6752 7307 9827
Size: 1255 2692 520 517 995 448 555 2520
Cuts at: 9827 9911 10154 11180 11546 13823 14351 16187 16497
Size: 84 243 1026 366 2277 528 1836 310
Cuts at: 16497 17025 18632 18649 18802 18987 19432 325
Size: 528 1607 17 153 185 445 1498
Fragments arranged by size:
2692 2520 2277 1836 1607 1498 1255 1026
995 555 528 528 520 517 448 445
366 310 243 185 153 84 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3429 3572 4912
Size: 274 80 33 51 1443 1452 143 1340
Cuts at: 4912 5148 5560 6030 7763 8357 9094 9712 11671
Size: 236 412 470 1733 594 737 618 1959
Cuts at: 11671 12693 17474 18851 20563 96
Size: 1022 4781 1377 1712 138
Fragments arranged by size:
4781 1959 1733 1712 1452 1443 1377 1340
1022 737 618 594 470 412 274 236
143 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2933 2965 3200 3232 8559 8591 8934 8966 9032
Size: 32 235 32 5327 32 343 32 66
Cuts at: 9032 9064 9330 9362 10283 10315 10540 10572 12711
Size: 32 266 32 921 32 225 32 2139
Cuts at: 12711 12743 13482 13514 14990 15022 20565 20597 2933
Size: 32 739 32 1476 32 5543 32 2941
Fragments arranged by size:
5543 5327 2941 2139 1476 921 739 343
266 235 225 66 32 32 32 32
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5435 13003 15827 15995 16070 16556 17761 1316
Size: 4119 7568 2824 168 75 486 1205 4160
Fragments arranged by size:
7568 4160 4119 2824 1205 486 168 75
XbaI T'CTAG_A
Cuts at: 3565 17941 3565
Size: 14376 6229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2509 4270 8323 11811 12658 2509
Size: 1761 4053 3488 847 10456
Fragments arranged by size:
10456 4053 3488 1761 847
XhoI C'TCGA_G
Cuts at: 1943 5792 16872 1943
Size: 3849 11080 5676
Fragments arranged by size:
11080 5676 3849
XmaI C'CCGG_G
Cuts at: 1870 2379 17418 17930 1870
Size: 509 15039 512 4545
Fragments arranged by size:
15039 4545 512 509
XmnI GAAnn'nnTTC
Cuts at: 2252 2687 3615 4441 4935 5001 6909 9117 10269
Size: 435 928 826 494 66 1908 2208 1152
Cuts at: 10269 12887 14213 16059 16407 18734 2252
Size: 2618 1326 1846 348 2327 4123
Fragments arranged by size:
4123 2618 2327 2208 1908 1846 1326 1152
928 826 494 435 348 66
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 18413 276
Size: 53 83 186 936 16879 2468
Fragments arranged by size:
16879 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BbeI BsiWI BstZ17I FseI FspAI KasI
NarI NruI PacI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI