Restriction map for
pFN21AE0599
(Circular) (Six-base) MAPSORT of: pFN21AE0599.seq Check: 2838 from: 1 to: 5281
pFN21AE0599 5281 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2535 3091 278
Size: 53 83 186 936 999 556 2468
Fragments arranged by size:
2468 999 936 556 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2624 1937
Size: 687 4594
Acc65I G'GTAC_C
Cuts at: 2602 2602
Size: 5281
AclI AA'CG_TT
Cuts at: 1563 3408 3781 1563
Size: 1845 373 3063
Fragments arranged by size:
3063 1845 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2371 3344 4728 4996 1632
Size: 739 973 1384 268 1917
Fragments arranged by size:
1917 1384 973 739 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5084
AflIII A'CryG_T
Cuts at: 1203 4180 5157 1203
Size: 2977 977 1327
Fragments arranged by size:
2977 1327 977
AgeI A'CCGG_T
Cuts at: 4112 4112
Size: 5281
AhdI GACnn_n'nnGTC
Cuts at: 4010 4010
Size: 5281
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5281
AlwNI CAG_nnn'CTG
Cuts at: 2222 2297 2316 3024 4596 2222
Size: 75 19 708 1572 2907
Fragments arranged by size:
2907 1572 708 75 19
ApaLI G'TGCA_C
Cuts at: 2270 3338 4494 2270
Size: 1068 1156 3057
Fragments arranged by size:
3057 1156 1068
ApoI r'AATT_y
Cuts at: 1493 2016 2590 2816 2968 5077 1493
Size: 523 574 226 152 2109 1697
Fragments arranged by size:
2109 1697 574 523 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3835 160
Size: 872 2803 1606
Fragments arranged by size:
2803 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
AvaI C'yCGr_G
Cuts at: 1870 1943 2606 1870
Size: 73 663 4545
Fragments arranged by size:
4545 663 73
BamHI G'GATC_C
Cuts at: 2257 2611 2978 2257
Size: 354 367 4560
Fragments arranged by size:
4560 367 354
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 2197 2602 4057 618
Size: 325 217 627 330 80 405 1455 1842
Fragments arranged by size:
1842 1455 627 405 330 325 217 80
BanII G_rGCy'C
Cuts at: 1391 2600 1391
Size: 1209 4072
BbeI G_GCGC'C
Cuts at: 2121 2121
Size: 5281
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3472 3506 1903
Size: 34 1535 34 3678
Fragments arranged by size:
3678 1535 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3173 4383 3173
Size: 1210 4071
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5281
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2286 3892
Size: 107 122 71 631 605 337 277 1606
Cuts at: 3892 136
Size: 1525
Fragments arranged by size:
1606 1525 631 605 337 277 122 107
71
BglII A'GATC_T
Cuts at: 5276 5276
Size: 5281
BlpI GC'TnA_GC
Cuts at: 2698 2698
Size: 5281
Bme1580I G_kGCm'C
Cuts at: 1790 2202 2274 3342 4498 1790
Size: 412 72 1068 1156 2573
Fragments arranged by size:
2573 1156 1068 412 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 3970 442
Size: 416 53 83 399 24 1595 958 1753
Fragments arranged by size:
1753 1595 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3941 1239
Size: 2702 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3406 4271 4569 3406
Size: 865 298 4118
Fragments arranged by size:
4118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3944 882
Size: 617 2445 2219
Fragments arranged by size:
2445 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3072 493
Size: 711 1868 2702
Fragments arranged by size:
2702 1868 711
BsaBI GATnn'nnATC
Cuts at: 5275 5275
Size: 5281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2118 2532 3088 3470
Size: 53 83 186 936 585 414 556 382
Cuts at: 3470 4105 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 585 556 414 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2055 3714 4112 4386 4533 4944 2055
Size: 1659 398 274 147 411 2392
Fragments arranged by size:
2392 1659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2026 2056 1178
Size: 30 165 30 623 30 4403
Fragments arranged by size:
4403 623 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2391 1186
Size: 1205 4076
BseYI C'CCAG_C
Cuts at: 4484 4980 4484
Size: 496 4785
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4708
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2731 3492 4520 1540
Size: 275 186 730 761 1028 2301
Fragments arranged by size:
2301 1028 761 730 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 3342 3427 4498 2274
Size: 1068 85 1071 3057
Fragments arranged by size:
3057 1071 1068 85
BsmI GAATG_Cn'
Cuts at: 2792 2885 2792
Size: 93 5188
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2274 2600 3342 3427 4498 1391
Size: 399 412 72 326 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 326 85 72
BspEI T'CCGG_A
Cuts at: 2055 4944 2055
Size: 2889 2392
BspHI T'CATG_A
Cuts at: 3170 4900 3170
Size: 1730 3551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2636 844
Size: 1792 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3168 1394
Size: 429 1345 3507
Fragments arranged by size:
3507 1345 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2354 3776 3950 65
Size: 1183 1106 1422 174 1396
Fragments arranged by size:
1422 1396 1183 1106 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 2540 3925 4112 1700
Size: 110 264 466 1385 187 2869
Fragments arranged by size:
2869 1385 466 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5281
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5281
BssSI C'ACGA_G
Cuts at: 2566 3341 4353 2566
Size: 775 1012 3494
Fragments arranged by size:
3494 1012 775
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5281
BstBI TT'CG_AA
Cuts at: 4085 4085
Size: 5281
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5281
BstYI r'GATC_y
Cuts at: 1972 2257 2611 2978 3363 3380 4832 4918 4930
Size: 285 354 367 385 17 1452 86 12
Cuts at: 4930 5276 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 354 346 285 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2555 2555
Size: 5281
BtgI C'CryG_G
Cuts at: 513 1065 2004 2281 2360 513
Size: 552 939 277 79 3434
Fragments arranged by size:
3434 939 552 277 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4542
Fragments arranged by size:
4542 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 3591 3611 2797
Size: 794 20 4467
Fragments arranged by size:
4467 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5246
DraI TTT'AAA
Cuts at: 2585 2585
Size: 5281
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4288 809
Size: 507 2972 1802
Fragments arranged by size:
2972 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2076 2278
Size: 54 1425 50 275 18 198 48 202
Cuts at: 2278 2728 3617 5246 8
Size: 450 889 1629 43
Fragments arranged by size:
1629 1425 889 450 275 202 198 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2728 1537
Size: 275 916 4090
Fragments arranged by size:
4090 916 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2493 3211 1353
Size: 710 430 718 3423
Fragments arranged by size:
3423 718 710 430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3868 4242 4388 387
Size: 1064 138 2279 374 146 1280
Fragments arranged by size:
2279 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2371 3344 3941 4728 4996 1239
Size: 393 739 973 597 787 268 1524
Fragments arranged by size:
1524 973 787 739 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2558 2558
Size: 5281
EcoO109I rG'GnC_Cy
Cuts at: 2219 2219
Size: 5281
EcoRI G'AATT_C
Cuts at: 2590 2968 2590
Size: 378 4903
FseI GG_CCGG'CC
Cuts at: 2078 2078
Size: 5281
FspI TGC'GCA
Cuts at: 2157 3787 2157
Size: 1630 3651
HaeII r_GCGC'y
Cuts at: 1678 2121 4428 1678
Size: 443 2307 2531
Fragments arranged by size:
2531 2307 443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3922 3954 3996 4028 1373
Size: 32 135 32 2350 32 42 32 2626
Fragments arranged by size:
2626 2350 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2625 2871 669
Size: 988 281 687 246 3079
Fragments arranged by size:
3079 988 687 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5281
HpaI GTT'AAC
Cuts at: 2871 2871
Size: 5281
KasI G'GCGC_C
Cuts at: 2117 2117
Size: 5281
KpnI G_GTAC'C
Cuts at: 2606 2606
Size: 5281
MfeI C'AATT_G
Cuts at: 2022 2880 2022
Size: 858 4423
MluI A'CGCG_T
Cuts at: 5157 5157
Size: 5281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4395 4579 1305
Size: 3090 184 2007
Fragments arranged by size:
3090 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 2280 10
Size: 54 1425 343 198 250 3011
Fragments arranged by size:
3011 1425 343 250 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2359 3239 3598 3757 518
Size: 648 1193 880 359 159 2042
Fragments arranged by size:
2042 1193 880 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2103 2697 3374 4522 4767 1606
Size: 497 594 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 594 497 245
NaeI GCC'GGC
Cuts at: 2076 2076
Size: 5281
NarI GG'CG_CC
Cuts at: 2118 2118
Size: 5281
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3790
Fragments arranged by size:
3790 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5281
NgoMIV G'CCGG_C
Cuts at: 2074 2074
Size: 5281
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5281
NotI GC'GGCC_GC
Cuts at: 2728 2728
Size: 5281
NspI r_CATG'y
Cuts at: 1132 1229 2639 4184 1132
Size: 97 1410 1545 2229
Fragments arranged by size:
2229 1545 1410 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5281
PciI A'CATG_T
Cuts at: 4180 4180
Size: 5281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5281
PfoI T'CCnGG_A
Cuts at: 2044 2044
Size: 5281
PmeI GTTT'AAAC
Cuts at: 2585 2585
Size: 5281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 4863 4895 3350
Size: 32 1481 32 3736
Fragments arranged by size:
3736 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2219 2219
Size: 5281
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5281
PsiI TTA'TAA
Cuts at: 2851 2851
Size: 5281
PstI C_TGCA'G
Cuts at: 830 2633 830
Size: 1803 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5281
SalI G'TCGA_C
Cuts at: 1936 2623 1936
Size: 687 4594
SbfI CC_TGCA'GG
Cuts at: 2633 2633
Size: 5281
ScaI AGT'ACT
Cuts at: 3529 5241 3529
Size: 1712 3569
SfcI C'TryA_G
Cuts at: 826 1045 2629 3764 4445 4636 826
Size: 219 1584 1135 681 191 1471
Fragments arranged by size:
1584 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2119 2119
Size: 5281
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5281
SmaI CCC'GGG
Cuts at: 1872 2608 1872
Size: 736 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3385 4286 4548 820
Size: 197 926 1442 901 262 1553
Fragments arranged by size:
1553 1442 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3072 493
Size: 2579 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5281
SphI G_CATG'C
Cuts at: 1132 2639 1132
Size: 1507 3774
SspI AAT'ATT
Cuts at: 5 52 3205 5
Size: 47 3153 2081
Fragments arranged by size:
3153 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5281
StyI C'CwwG_G
Cuts at: 513 1065 2004 2720 513
Size: 552 939 716 3074
Fragments arranged by size:
3074 939 716 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3308 3325 3478 3663 4108 325
Size: 1255 1728 17 153 185 445 1498
Fragments arranged by size:
1728 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3527 5239 96
Size: 274 80 33 51 1443 1550 1712 138
Fragments arranged by size:
1712 1550 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2026 2058 5241 5273 2026
Size: 32 3183 32 2034
Fragments arranged by size:
3183 2034 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2534 1316
Size: 1218 4063
XbaI T'CTAG_A
Cuts at: 2617 2617
Size: 5281
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5281
XmaI C'CCGG_G
Cuts at: 1870 2606 1870
Size: 736 4545
XmnI GAAnn'nnTTC
Cuts at: 3410 3410
Size: 5281
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2533 3089 276
Size: 53 83 186 936 999 556 2468
Fragments arranged by size:
2468 999 936 556 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoRV FalI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI