Restriction map for
pFN21AE0589
(Circular) (Six-base) MAPSORT of: pFN21AE0589.seq Check: 8512 from: 1 to: 6091
pFN21AE0589 6091 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4154 5538 5806 1632
Size: 2522 1384 268 1917
Fragments arranged by size:
2522 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2898 3144 4990 5967 1203
Size: 1695 246 1846 977 1327
Fragments arranged by size:
1846 1695 1327 977 246
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 2257 3834 5406 2257
Size: 1577 1572 2942
Fragments arranged by size:
2942 1577 1572
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2549 2549
Size: 6091
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 3054 3120 3416 1870
Size: 73 1111 66 296 4545
Fragments arranged by size:
4545 1111 296 73 66
BamHI G'GATC_C
Cuts at: 2266 3421 3788 2266
Size: 1155 367 4569
Fragments arranged by size:
4569 1155 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2680 3115 3412 4867 618
Size: 325 217 627 893 435 297 1455 1842
Fragments arranged by size:
1842 1455 893 627 435 325 297 217
BanII G_rGCy'C
Cuts at: 1391 2953 3410 1391
Size: 1562 457 4072
Fragments arranged by size:
4072 1562 457
BbeI G_GCGC'C
Cuts at: 3119 3119
Size: 6091
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 3118 928
Size: 971 213 1006 3901
Fragments arranged by size:
3901 1006 971 213
BbvCI CC'TCA_GC
Cuts at: 2171 2171
Size: 6091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2411 3508 2411
Size: 1097 4994
Bme1580I G_kGCm'C
Cuts at: 1790 2685 4152 5308 1790
Size: 895 1467 1156 2573
Fragments arranged by size:
2573 1467 1156 895
BmgBI CAC'GTC
Cuts at: 1318 2401 1318
Size: 1083 5008
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2215 2735 3193 4751 1239
Size: 976 520 458 1558 2579
Fragments arranged by size:
2579 1558 976 520 458
Bpu10I CC'TnA_GC
Cuts at: 2171 2171
Size: 6091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 4216 5081 5379 2927
Size: 1289 865 298 3639
Fragments arranged by size:
3639 1289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2181 4754 882
Size: 617 682 2573 2219
Fragments arranged by size:
2573 2219 682 617
BsaAI yAC'GTr
Cuts at: 493 1204 3151 3882 493
Size: 711 1947 731 2702
Fragments arranged by size:
2702 1947 731 711
BsaBI GATnn'nnATC
Cuts at: 2750 6085 2750
Size: 3335 2756
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2472 3116 3898 4280
Size: 53 83 186 936 939 644 782 382
Cuts at: 4280 4915 275
Size: 635 1451
Fragments arranged by size:
1451 939 936 782 644 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5866
Fragments arranged by size:
5866 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3043 1186
Size: 1857 4234
BseYI C'CCAG_C
Cuts at: 2199 2503 5294 5790 2199
Size: 304 2791 496 2500
Fragments arranged by size:
2791 2500 496 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2780 3295 1163
Size: 573 1044 515 3959
Fragments arranged by size:
3959 1044 573 515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3071 4152 4237 5308 3071
Size: 1081 85 1071 3854
Fragments arranged by size:
3854 1081 1071 85
BsmI GAATG_Cn'
Cuts at: 2143 3602 3695 2143
Size: 1459 93 4539
Fragments arranged by size:
4539 1459 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2685 2953 3071 3410 4152 4237 5308
Size: 399 895 268 118 339 742 85 1071
Cuts at: 5308 1391
Size: 2174
Fragments arranged by size:
2174 1071 895 742 399 339 268 118
85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3312 3446 844
Size: 2468 134 3489
Fragments arranged by size:
3489 2468 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2838 4586 4760 65
Size: 1183 1590 1748 174 1396
Fragments arranged by size:
1748 1590 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2870 3018 4735 4922 1700
Size: 110 1060 148 1717 187 2869
Fragments arranged by size:
2869 1717 1060 187 148 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 2549 1925
Size: 624 5467
BssSI C'ACGA_G
Cuts at: 2373 4151 5163 2373
Size: 1778 1012 3301
Fragments arranged by size:
3301 1778 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 2995 1184
Size: 1811 4280
BstXI CCAn_nnnn'nTGG
Cuts at: 2713 2929 3082 3208 2713
Size: 216 153 126 5596
Fragments arranged by size:
5596 216 153 126
BstYI r'GATC_y
Cuts at: 1972 2037 2266 3421 3788 4173 4190 5642 5728
Size: 65 229 1155 367 385 17 1452 86
Cuts at: 5728 5740 6086 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1155 385 367 346 229 86
65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2025 513
Size: 552 960 4579
Fragments arranged by size:
4579 960 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2334 487
Size: 683 56 1108 4244
Fragments arranged by size:
4244 1108 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 2544 2670 2775 3607 4401 4421 2070
Size: 474 126 105 832 794 20 3740
Fragments arranged by size:
3740 832 794 474 126 105 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3176 5098 809
Size: 507 1860 1922 1802
Fragments arranged by size:
1922 1860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2365 2435 2703
Size: 54 1425 50 275 18 535 70 268
Cuts at: 2703 3006 3198 3210 3538 4427 6056 8
Size: 303 192 12 328 889 1629 43
Fragments arranged by size:
1629 1425 889 535 328 303 275 268
192 70 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4021 1353
Size: 2668 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2231 4678 5052 5198 387
Size: 1064 138 642 2447 374 146 1280
Fragments arranged by size:
2447 1280 1064 642 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2735 3193 4154 4751 5538 5806
Size: 393 583 520 458 961 597 787 268
Cuts at: 5806 1239
Size: 1524
Fragments arranged by size:
1524 961 787 597 583 520 458 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2654 2654
Size: 6091
EcoO109I rG'GnC_Cy
Cuts at: 2047 2203 2342 2454 2574 2938 2047
Size: 156 139 112 120 364 5200
Fragments arranged by size:
5200 364 156 139 120 112
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
EcoRV GAT'ATC
Cuts at: 2614 2614
Size: 6091
FspI TGC'GCA
Cuts at: 2699 4597 2699
Size: 1898 4193
FspAI rTGC'GCAy
Cuts at: 2699 2699
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2600 3119 5238 1678
Size: 922 519 2119 2531
Fragments arranged by size:
2531 2119 922 519
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2900 2932 3273 3305 3346
Size: 32 135 32 1328 32 341 32 41
Cuts at: 3346 3378 4732 4764 4806 4838 1373
Size: 32 1354 32 42 32 2626
Fragments arranged by size:
2626 1354 1328 341 135 42 41 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2767 2868 3435 3681 669
Size: 988 281 829 101 567 246 3079
Fragments arranged by size:
3079 988 829 567 281 246 101
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KasI G'GCGC_C
Cuts at: 3115 3115
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3304 5205 5389 1305
Size: 1999 1901 184 2007
Fragments arranged by size:
2007 1999 1901 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2367 2437 3200 3212 10
Size: 54 1425 343 535 70 763 12 2889
Fragments arranged by size:
2889 1425 763 535 343 70 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 3149 4049 4408 4567 518
Size: 648 1458 525 900 359 159 2042
Fragments arranged by size:
2042 1458 900 648 525 359 159
MspA1I CmG'CkG
Cuts at: 1606 2315 2363 2406 3507 4184 5332 5577 1606
Size: 709 48 43 1101 677 1148 245 2120
Fragments arranged by size:
2120 1148 1101 709 677 245 48 43
NarI GG'CG_CC
Cuts at: 3116 3116
Size: 6091
NcoI C'CATG_G
Cuts at: 513 1065 2025 513
Size: 552 960 4579
Fragments arranged by size:
4579 960 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2902 3148 3449 4994 1132
Size: 97 1673 246 301 1545 2229
Fragments arranged by size:
2229 1673 1545 301 246 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 2898 3144 4990 2898
Size: 246 1846 3999
Fragments arranged by size:
3999 1846 246
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2692 1108
Size: 1584 4507
PfoI T'CCnGG_A
Cuts at: 2712 3364 2712
Size: 652 5439
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2203 2342 2454 2938 2047
Size: 156 139 112 484 5200
Fragments arranged by size:
5200 484 156 139 112
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 2064 2658 3051 3443 830
Size: 1234 594 393 392 3478
Fragments arranged by size:
3478 1234 594 393 392
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2315 2363 2315
Size: 48 6043
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SanDI GG'GwC_CC
Cuts at: 2047 2342 2047
Size: 295 5796
SbfI CC_TGCA'GG
Cuts at: 2064 2658 3443 2064
Size: 594 785 4712
Fragments arranged by size:
4712 785 594
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2060 2491 2654 3047 3439 4574 5255
Size: 219 1015 431 163 393 392 1135 681
Cuts at: 5255 5446 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1015 681 431 393 392 219
191 163
SfoI GGC'GCC
Cuts at: 3117 3117
Size: 6091
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3056 3122 3418 1872
Size: 1184 66 296 4545
Fragments arranged by size:
4545 1184 296 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2942 4195 5096 5358 820
Size: 197 926 999 1253 901 262 1553
Fragments arranged by size:
1553 1253 999 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2025 2223 2296 3189 3530 513
Size: 552 960 198 73 893 341 3074
Fragments arranged by size:
3074 960 893 552 341 198 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2935 4118 4135 4288 4473 4918 325
Size: 1255 1355 1183 17 153 185 445 1498
Fragments arranged by size:
1498 1355 1255 1183 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4337 6049 96
Size: 274 80 33 51 1443 2360 1712 138
Fragments arranged by size:
2360 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 2936 1316
Size: 1620 4471
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3054 3120 3416 1870
Size: 1184 66 296 4545
Fragments arranged by size:
4545 1184 296 66
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI XcmI