Restriction map for
pFN21AE0583
(Circular) (Six-base) MAPSORT of: pFN21AE0583.seq Check: 8943 from: 1 to: 5521
pFN21AE0583 5521 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2702 3331 278
Size: 53 83 186 936 1166 629 2468
Fragments arranged by size:
2468 1166 936 629 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2319 3584 4968 5236 1632
Size: 687 1265 1384 268 1917
Fragments arranged by size:
1917 1384 1265 687 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 4420 5397 1203
Size: 3217 977 1327
Fragments arranged by size:
3217 1327 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5521
AlwNI CAG_nnn'CTG
Cuts at: 3264 4836 3264
Size: 1572 3949
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2418 2830 3056 3208 5317 1493
Size: 925 412 226 152 2109 1697
Fragments arranged by size:
2109 1697 925 412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2846 1870
Size: 73 903 4545
Fragments arranged by size:
4545 903 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2842 4297 618
Size: 325 217 627 263 792 1455 1842
Fragments arranged by size:
1842 1455 792 627 325 263 217
BanII G_rGCy'C
Cuts at: 1391 2073 2479 2781 2840 1391
Size: 682 406 302 59 4072
Fragments arranged by size:
4072 682 406 302 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2531 928
Size: 971 632 3918
Fragments arranged by size:
3918 971 632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3712 3746 1903
Size: 34 1775 34 3678
Fragments arranged by size:
3678 1775 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2462 3413 4623 2462
Size: 951 1210 3360
Fragments arranged by size:
3360 1210 951
BclI T'GATC_A
Cuts at: 1548 2754 1548
Size: 1206 4315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2616 4132 136
Size: 107 122 71 631 605 944 1516 1525
Fragments arranged by size:
1525 1516 944 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2938 2938
Size: 5521
Bme1580I G_kGCm'C
Cuts at: 1790 3582 4738 1790
Size: 1792 1156 2573
Fragments arranged by size:
2573 1792 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3252 4210 442
Size: 416 53 83 399 24 1835 958 1753
Fragments arranged by size:
1835 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4181 1239
Size: 2942 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2564 4184 882
Size: 617 1065 1620 2219
Fragments arranged by size:
2219 1620 1065 617
BsaAI yAC'GTr
Cuts at: 493 1204 3312 493
Size: 711 2108 2702
Fragments arranged by size:
2702 2108 711
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2699 3328 3710 4345
Size: 53 83 186 936 1166 629 382 635
Cuts at: 4345 275
Size: 1451
Fragments arranged by size:
1451 1166 936 635 629 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3954 4352 4626 4773 5184 3954
Size: 398 274 147 411 4291
Fragments arranged by size:
4291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5296
Fragments arranged by size:
5296 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2756 1186
Size: 1570 3951
BseYI C'CCAG_C
Cuts at: 2538 2544 4724 5220 2538
Size: 6 2180 496 2839
Fragments arranged by size:
2839 2180 496 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4948
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2971 3732 4760 1540
Size: 275 186 970 761 1028 2301
Fragments arranged by size:
2301 1028 970 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 3582 3667 4738 2073
Size: 1509 85 1071 2856
Fragments arranged by size:
2856 1509 1071 85
BsmI GAATG_Cn'
Cuts at: 2159 3032 3125 2159
Size: 873 93 4555
Fragments arranged by size:
4555 873 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2690 2690
Size: 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2479 2781 2840 3582 3667 4738
Size: 399 283 406 302 59 742 85 1071
Cuts at: 4738 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 406 399 302 283 85
59
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 2876 844
Size: 1181 851 3489
Fragments arranged by size:
3489 1181 851
BsrBI CCG'CTC
Cuts at: 1394 1823 3408 1394
Size: 429 1585 3507
Fragments arranged by size:
3507 1585 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2735 4165 4352 1700
Size: 110 925 1430 187 2869
Fragments arranged by size:
2869 1430 925 187 110
BsrGI T'GTAC_A
Cuts at: 96 2204 96
Size: 2108 3413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5521
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5521
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2094 2851 3218 3603 3620 5072 5158 5170
Size: 122 757 367 385 17 1452 86 12
Cuts at: 5170 5516 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 757 385 367 346 122 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2601 2601
Size: 5521
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 2393 2518 2691 513
Size: 552 939 114 275 125 173 3343
Fragments arranged by size:
3343 939 552 275 173 125 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 2374 2736 3037 3831 3851 2374
Size: 362 301 794 20 4044
Fragments arranged by size:
4044 794 362 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2733 2968 3857
Size: 54 1425 50 275 18 903 235 889
Cuts at: 3857 5486 8
Size: 1629 43
Fragments arranged by size:
1629 1425 903 889 275 235 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2968 1537
Size: 275 1156 4090
Fragments arranged by size:
4090 1156 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 2798 3451 1353
Size: 1233 212 653 3423
Fragments arranged by size:
3423 1233 653 212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4108 4482 4628 387
Size: 1064 138 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2071 2071
Size: 5521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2319 3584 4181 4968 5236 1239
Size: 393 687 1265 597 787 268 1524
Fragments arranged by size:
1524 1265 787 687 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2489 2489
Size: 5521
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2381 2381
Size: 5521
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 4668 1678
Size: 2990 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2407 2439 4162 4194 4236
Size: 32 135 32 835 32 1723 32 42
Cuts at: 4236 4268 1373
Size: 32 2626
Fragments arranged by size:
2626 1723 835 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3699
Fragments arranged by size:
3699 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2048 2327 3479 3838 3997 518
Size: 648 882 279 1152 359 159 2042
Fragments arranged by size:
2042 1152 882 648 359 279 159
MspA1I CmG'CkG
Cuts at: 1606 2791 2937 3614 4762 5007 1606
Size: 1185 146 677 1148 245 2120
Fragments arranged by size:
2120 1185 1148 677 245 146
NaeI GCC'GGC
Cuts at: 2737 2737
Size: 5521
NcoI C'CATG_G
Cuts at: 513 1065 2004 2118 2393 2691 513
Size: 552 939 114 275 298 3343
Fragments arranged by size:
3343 939 552 298 275 114
NdeI CA'TA_TG
Cuts at: 387 2552 387
Size: 2165 3356
NgoMIV G'CCGG_C
Cuts at: 2735 2735
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2489 2489
Size: 5521
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PstI C_TGCA'G
Cuts at: 830 2381 2873 830
Size: 1551 492 3478
Fragments arranged by size:
3478 1551 492
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
SacI G_AGCT'C
Cuts at: 2073 2073
Size: 5521
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SexAI A'CCwGG_T
Cuts at: 2721 2721
Size: 5521
SfcI C'TryA_G
Cuts at: 826 1045 2377 2869 4004 4685 4876 826
Size: 219 1332 492 1135 681 191 1471
Fragments arranged by size:
1471 1332 1135 681 492 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2848 1872
Size: 976 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3625 4526 4788 820
Size: 197 926 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2118 2393 2691 2960 513
Size: 552 939 114 275 298 269 3074
Fragments arranged by size:
3074 939 552 298 275 269 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3548 3565 3718 3903 4348 325
Size: 1255 1968 17 153 185 445 1498
Fragments arranged by size:
1968 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2204 3767 5479
Size: 274 80 33 51 1443 227 1563 1712
Cuts at: 5479 96
Size: 138
Fragments arranged by size:
1712 1563 1443 274 227 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5481 5513 5481
Size: 32 5489
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2846 1870
Size: 976 4545
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2700 3329 276
Size: 53 83 186 936 1166 629 2468
Fragments arranged by size:
2468 1166 936 629 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI