Restriction map for
pFN21AE0578
(Circular) (Six-base) MAPSORT of: pFN21AE0578.seq Check: 6641 from: 1 to: 5326
pFN21AE0578 5326 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3136 278
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 1937
Size: 732 4594
Acc65I G'GTAC_C
Cuts at: 2647 2647
Size: 5326
AclI AA'CG_TT
Cuts at: 1563 3453 3826 1563
Size: 1890 373 3063
Fragments arranged by size:
3063 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 3389 4773 5041 1632
Size: 560 1197 1384 268 1917
Fragments arranged by size:
1917 1384 1197 560 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5129
AflIII A'CryG_T
Cuts at: 1203 2393 4225 5202 1203
Size: 1190 1832 977 1327
Fragments arranged by size:
1832 1327 1190 977
AgeI A'CCGG_T
Cuts at: 4157 4157
Size: 5326
AhdI GACnn_n'nnGTC
Cuts at: 4055 4055
Size: 5326
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5326
AlwNI CAG_nnn'CTG
Cuts at: 3069 4641 3069
Size: 1572 3754
ApaLI G'TGCA_C
Cuts at: 2246 3383 4539 2246
Size: 1137 1156 3033
Fragments arranged by size:
3033 1156 1137
ApoI r'AATT_y
Cuts at: 1493 2549 2635 2861 3013 5122 1493
Size: 1056 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 1056 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 3880 160
Size: 872 2848 1606
Fragments arranged by size:
2848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 2651 1870
Size: 73 574 134 4545
Fragments arranged by size:
4545 574 134 73
AvrII C'CTAG_G
Cuts at: 2347 2347
Size: 5326
BamHI G'GATC_C
Cuts at: 2178 2656 3023 2178
Size: 478 367 4481
Fragments arranged by size:
4481 478 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2187 2647 4102 618
Size: 325 217 627 400 460 1455 1842
Fragments arranged by size:
1842 1455 627 460 400 325 217
BanII G_rGCy'C
Cuts at: 1391 2645 1391
Size: 1254 4072
BbeI G_GCGC'C
Cuts at: 2191 2191
Size: 5326
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2017 928
Size: 971 118 4237
Fragments arranged by size:
4237 971 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3517 3551 1903
Size: 34 1580 34 3678
Fragments arranged by size:
3678 1580 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2519 3218 4428 2519
Size: 699 1210 3417
Fragments arranged by size:
3417 1210 699
BclI T'GATC_A
Cuts at: 1548 2313 1548
Size: 765 4561
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2211 3937 136
Size: 107 122 71 631 605 539 1726 1525
Fragments arranged by size:
1726 1525 631 605 539 122 107 71
BglII A'GATC_T
Cuts at: 2226 5321 2226
Size: 3095 2231
BlpI GC'TnA_GC
Cuts at: 2743 2743
Size: 5326
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2250 3387 4543 1790
Size: 292 168 1137 1156 2573
Fragments arranged by size:
2573 1156 1137 292 168
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5326
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2050 3057 4015
Size: 416 53 83 399 24 633 1007 958
Cuts at: 4015 442
Size: 1753
Fragments arranged by size:
1753 1007 958 633 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2387 2387
Size: 5326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 2306 3986 1239
Size: 1004 63 1680 2579
Fragments arranged by size:
2579 1680 1004 63
Bpu10I CC'TnA_GC
Cuts at: 2083 2083
Size: 5326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3451 4316 4614 3451
Size: 865 298 4163
Fragments arranged by size:
4163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2601 3989 882
Size: 617 1102 1388 2219
Fragments arranged by size:
2219 1388 1102 617
BsaAI yAC'GTr
Cuts at: 493 1204 2396 2491 3117 493
Size: 711 1192 95 626 2702
Fragments arranged by size:
2702 1192 711 626 95
BsaBI GATnn'nnATC
Cuts at: 2318 5320 2318
Size: 3002 2324
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 2188 3133 3515
Size: 53 83 186 936 489 166 945 382
Cuts at: 3515 4150 275
Size: 635 1451
Fragments arranged by size:
1451 945 936 635 489 382 186 166
83 53
BsaWI w'CCGG_w
Cuts at: 3759 4157 4431 4578 4989 3759
Size: 398 274 147 411 4096
Fragments arranged by size:
4096 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 1178
Size: 30 165 30 639 30 4432
Fragments arranged by size:
4432 639 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2050 2379 1186
Size: 864 329 4133
Fragments arranged by size:
4133 864 329
BseYI C'CCAG_C
Cuts at: 4529 5025 4529
Size: 496 4830
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4753
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2776 3537 4565 1540
Size: 275 186 775 761 1028 2301
Fragments arranged by size:
2301 1028 775 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2250 3387 3472 4543 2250
Size: 1137 85 1071 3033
Fragments arranged by size:
3033 1137 1071 85
BsmI GAATG_Cn'
Cuts at: 2837 2930 2837
Size: 93 5233
BsmBI CGTCTCn'nnnn_
Cuts at: 2070 2070
Size: 5326
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2250 2645 3387 3472 4543 1391
Size: 399 292 168 395 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 395 292 168 85
BspEI T'CCGG_A
Cuts at: 4989 4989
Size: 5326
BspHI T'CATG_A
Cuts at: 3215 4945 3215
Size: 1730 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2681 844
Size: 1837 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3213 1394
Size: 429 1390 3507
Fragments arranged by size:
3507 1390 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3821 3995 65
Size: 1183 2573 174 1396
Fragments arranged by size:
2573 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2210 3970 4157 1700
Size: 110 400 1760 187 2869
Fragments arranged by size:
2869 1760 400 187 110
BsrGI T'GTAC_A
Cuts at: 96 2457 96
Size: 2361 2965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5326
BssSI C'ACGA_G
Cuts at: 3386 4398 3386
Size: 1012 4314
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5326
BstBI TT'CG_AA
Cuts at: 4130 4130
Size: 5326
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5326
BstYI r'GATC_y
Cuts at: 1972 2044 2178 2226 2578 2656 3023 3408 3425
Size: 72 134 48 352 78 367 385 17
Cuts at: 3425 4877 4963 4975 5321 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 352 346 134 86
78 72 48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3835
Fragments arranged by size:
3835 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4587
Fragments arranged by size:
4587 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3636 3656 2842
Size: 794 20 4512
Fragments arranged by size:
4512 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5291
DraI TTT'AAA
Cuts at: 2630 2630
Size: 5326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4333 809
Size: 507 3017 1802
Fragments arranged by size:
3017 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2773 3662 5291
Size: 54 1425 50 275 18 943 889 1629
Cuts at: 5291 8
Size: 43
Fragments arranged by size:
1629 1425 943 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2773 1537
Size: 275 961 4090
Fragments arranged by size:
4090 961 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3256 1353
Size: 1903 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3913 4287 4433 387
Size: 1064 138 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2243 2306 3389 3986 4773 5041
Size: 393 560 51 63 1083 597 787 268
Cuts at: 5041 1239
Size: 1524
Fragments arranged by size:
1524 1083 787 597 560 393 268 63
51
EcoO109I rG'GnC_Cy
Cuts at: 2253 2253
Size: 5326
EcoRI G'AATT_C
Cuts at: 2635 3013 2635
Size: 378 4948
FspI TGC'GCA
Cuts at: 3832 3832
Size: 5326
HaeII r_GCGC'y
Cuts at: 1678 2116 2191 4473 1678
Size: 438 75 2282 2531
Fragments arranged by size:
2531 2282 438 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3967 3999 4041 4073 1373
Size: 32 135 32 2395 32 42 32 2626
Fragments arranged by size:
2626 2395 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 2916 669
Size: 988 281 732 246 3079
Fragments arranged by size:
3079 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5326
HpaI GTT'AAC
Cuts at: 2916 2916
Size: 5326
KasI G'GCGC_C
Cuts at: 2187 2187
Size: 5326
KpnI G_GTAC'C
Cuts at: 2651 2651
Size: 5326
MfeI C'AATT_G
Cuts at: 2925 2925
Size: 5326
MluI A'CGCG_T
Cuts at: 5202 5202
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4440 4624 1305
Size: 3135 184 2007
Fragments arranged by size:
3135 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3504
Fragments arranged by size:
3504 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2398 3284 3643 3802 518
Size: 648 1232 886 359 159 2042
Fragments arranged by size:
2042 1232 886 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2742 3419 4567 4812 1606
Size: 1136 677 1148 245 2120
Fragments arranged by size:
2120 1148 1136 677 245
NarI GG'CG_CC
Cuts at: 2188 2188
Size: 5326
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3835
Fragments arranged by size:
3835 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5326
NotI GC'GGCC_GC
Cuts at: 2773 2773
Size: 5326
NspI r_CATG'y
Cuts at: 1132 1229 2684 4229 1132
Size: 97 1455 1545 2229
Fragments arranged by size:
2229 1545 1455 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5326
PciI A'CATG_T
Cuts at: 4225 4225
Size: 5326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5326
PfoI T'CCnGG_A
Cuts at: 2283 2409 2283
Size: 126 5200
PmeI GTTT'AAAC
Cuts at: 2630 2630
Size: 5326
PmlI CAC'GTG
Cuts at: 2396 2396
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 4908 4940 3395
Size: 32 1481 32 3781
Fragments arranged by size:
3781 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2253 2253
Size: 5326
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5326
PsiI TTA'TAA
Cuts at: 2896 2896
Size: 5326
PstI C_TGCA'G
Cuts at: 830 2142 2678 830
Size: 1312 536 3478
Fragments arranged by size:
3478 1312 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5326
SalI G'TCGA_C
Cuts at: 1936 2668 1936
Size: 732 4594
SanDI GG'GwC_CC
Cuts at: 2253 2253
Size: 5326
SbfI CC_TGCA'GG
Cuts at: 2678 2678
Size: 5326
ScaI AGT'ACT
Cuts at: 3574 5286 3574
Size: 1712 3614
SfcI C'TryA_G
Cuts at: 826 1045 2138 2574 2620 2674 3809 4490 4681
Size: 219 1093 436 46 54 1135 681 191
Cuts at: 4681 826
Size: 1471
Fragments arranged by size:
1471 1135 1093 681 436 219 191 54
46
SfoI GGC'GCC
Cuts at: 2189 2189
Size: 5326
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5326
SmaI CCC'GGG
Cuts at: 1872 2519 2653 1872
Size: 647 134 4545
Fragments arranged by size:
4545 647 134
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3430 4331 4593 820
Size: 197 926 1487 901 262 1553
Fragments arranged by size:
1553 1487 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3117 493
Size: 2624 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5326
SphI G_CATG'C
Cuts at: 1132 2684 1132
Size: 1552 3774
SspI AAT'ATT
Cuts at: 5 52 3250 5
Size: 47 3198 2081
Fragments arranged by size:
3198 2081 47
StuI AGG'CCT
Cuts at: 1517 2572 1517
Size: 1055 4271
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2347 2765 513
Size: 552 939 30 313 418 3074
Fragments arranged by size:
3074 939 552 418 313 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2167 3353 3370 3523 3708 4153 325
Size: 1255 587 1186 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1186 587 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2457 3572 5284
Size: 274 80 33 51 1443 480 1115 1712
Cuts at: 5284 96
Size: 138
Fragments arranged by size:
1712 1443 1115 480 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 5286 5318 2042
Size: 32 3212 32 2050
Fragments arranged by size:
3212 2050 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5326
XbaI T'CTAG_A
Cuts at: 2662 2662
Size: 5326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5326
XmaI C'CCGG_G
Cuts at: 1870 2517 2651 1870
Size: 647 134 4545
Fragments arranged by size:
4545 647 134
XmnI GAAnn'nnTTC
Cuts at: 3455 3455
Size: 5326
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3134 276
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI