Restriction map for
pFN21AE0573
(Circular) (Six-base) MAPSORT of: pFN21AE0573.seq Check: 3786 from: 1 to: 5572
pFN21AE0573 5572 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3382 278
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2451 2915 1937
Size: 514 464 4594
Fragments arranged by size:
4594 514 464
Acc65I G'GTAC_C
Cuts at: 2893 2893
Size: 5572
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2488 3635 5019 5287 1632
Size: 856 1147 1384 268 1917
Fragments arranged by size:
1917 1384 1147 856 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 4471 5448 1203
Size: 3268 977 1327
Fragments arranged by size:
3268 1327 977
AgeI A'CCGG_T
Cuts at: 4403 4403
Size: 5572
AhdI GACnn_n'nnGTC
Cuts at: 4301 4301
Size: 5572
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5572
AlwNI CAG_nnn'CTG
Cuts at: 2078 2263 2377 2511 3315 4887 2078
Size: 185 114 134 804 1572 2763
Fragments arranged by size:
2763 1572 804 185 134 114
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2881 3107 3259 5368 1493
Size: 1388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2417 2897 1870
Size: 73 74 400 480 4545
Fragments arranged by size:
4545 480 400 74 73
BamHI G'GATC_C
Cuts at: 2902 3269 2902
Size: 367 5205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2357 2740 2893 4348 618
Size: 325 217 627 570 383 153 1455 1842
Fragments arranged by size:
1842 1455 627 570 383 325 217 153
BanII G_rGCy'C
Cuts at: 1391 2384 2480 2603 2833 2891 1391
Size: 993 96 123 230 58 4072
Fragments arranged by size:
4072 993 230 123 96 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2187 928
Size: 971 288 4313
Fragments arranged by size:
4313 971 288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 1903
Size: 34 1826 34 3678
Fragments arranged by size:
3678 1826 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3464 4674 3464
Size: 1210 4362
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2766 4183 136
Size: 107 122 71 631 605 1094 1417 1525
Fragments arranged by size:
1525 1417 1094 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5567 5567
Size: 5572
BlpI GC'TnA_GC
Cuts at: 2378 2989 2378
Size: 611 4961
Bme1580I G_kGCm'C
Cuts at: 1790 2246 2360 2743 3633 4789 1790
Size: 456 114 383 890 1156 2573
Fragments arranged by size:
2573 1156 890 456 383 114
BmgBI CAC'GTC
Cuts at: 1318 2863 1318
Size: 1545 4027
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3303 4261 442
Size: 416 53 83 399 24 1886 958 1753
Fragments arranged by size:
1886 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5572
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2043 2043
Size: 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2240 2285 2307 2513 2716 2747 4232
Size: 803 198 45 22 206 203 31 1485
Cuts at: 4232 1239
Size: 2579
Fragments arranged by size:
2579 1485 803 206 203 198 45 31
22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3697 4562 4860 3697
Size: 865 298 4409
Fragments arranged by size:
4409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2071 2102 2381 4235 882
Size: 617 572 31 279 1854 2219
Fragments arranged by size:
2219 1854 617 572 279 31
BsaAI yAC'GTr
Cuts at: 493 1204 2455 3363 493
Size: 711 1251 908 2702
Fragments arranged by size:
2702 1251 908 711
BsaBI GATnn'nnATC
Cuts at: 5566 5566
Size: 5572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2312 2804 2819 3379
Size: 53 83 186 936 779 492 15 560
Cuts at: 3379 3761 4396 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 779 635 560 492 382 186
83 53 15
BsaWI w'CCGG_w
Cuts at: 2799 4005 4403 4677 4824 5235 2799
Size: 1206 398 274 147 411 3136
Fragments arranged by size:
3136 1206 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 2714 2744 1178
Size: 30 165 30 1059 30 222 30 4006
Fragments arranged by size:
4006 1059 222 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2165 2174 2366 2435 2501 1186
Size: 873 106 9 192 69 66 4257
Fragments arranged by size:
4257 873 192 106 69 66 9
BseYI C'CCAG_C
Cuts at: 2091 4775 5271 2091
Size: 2684 496 2392
Fragments arranged by size:
2684 2392 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 2627 1163
Size: 573 615 276 4108
Fragments arranged by size:
4108 615 573 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2367 2837 3022 3783 4811 1540
Size: 275 186 366 470 185 761 1028 2301
Fragments arranged by size:
2301 1028 761 470 366 275 186 185
BsiHKAI G_wGCw'C
Cuts at: 3633 3718 4789 3633
Size: 85 1071 4416
Fragments arranged by size:
4416 1071 85
BsmI GAATG_Cn'
Cuts at: 3083 3176 3083
Size: 93 5479
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2246 2360 2384 2480 2603 2743 2833
Size: 399 456 114 24 96 123 140 90
Cuts at: 2833 2891 3633 3718 4789 1391
Size: 58 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 456 399 140 123 114
96 90 85 58 24
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 3461 5191 3461
Size: 1730 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2927 844
Size: 2083 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 3459 1394
Size: 429 202 1434 3507
Fragments arranged by size:
3507 1434 429 202
BsrDI GCAATG_nn'
Cuts at: 65 1248 2246 4067 4241 65
Size: 1183 998 1821 174 1396
Fragments arranged by size:
1821 1396 1183 998 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2806 4216 4403 1700
Size: 110 996 1410 187 2869
Fragments arranged by size:
2869 1410 996 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5572
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5572
BssSI C'ACGA_G
Cuts at: 3632 4644 3632
Size: 1012 4560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5572
BstBI TT'CG_AA
Cuts at: 4376 4376
Size: 5572
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5572
BstYI r'GATC_y
Cuts at: 1972 2444 2902 3269 3654 3671 5123 5209 5221
Size: 472 458 367 385 17 1452 86 12
Cuts at: 5221 5567 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 472 458 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2079 2079
Size: 5572
BtgI C'CryG_G
Cuts at: 513 1065 2292 2315 2361 2391 2616 2704 513
Size: 552 1227 23 46 30 225 88 3381
Fragments arranged by size:
3381 1227 552 225 88 46 30 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4833
Fragments arranged by size:
4833 683 56
BtsI GCAGTG_nn'
Cuts at: 3088 3882 3902 3088
Size: 794 20 4758
Fragments arranged by size:
4758 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2876 2876
Size: 5572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4579 809
Size: 507 3263 1802
Fragments arranged by size:
3263 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 2834 3019
Size: 54 1425 50 275 18 534 470 185
Cuts at: 3019 3908 5537 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 534 470 275 185 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2364 2834 3019 1537
Size: 275 552 470 185 4090
Fragments arranged by size:
4090 552 470 275 185
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2160 2343 2642 3502 1353
Size: 683 124 183 299 860 3423
Fragments arranged by size:
3423 860 683 299 183 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2470 4159 4533 4679 387
Size: 1064 138 881 1689 374 146 1280
Fragments arranged by size:
1689 1280 1064 881 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2240 2285 2307 2488 2513 2716
Size: 393 410 198 45 22 181 25 203
Cuts at: 2716 2747 3635 4232 5019 5287 1239
Size: 31 888 597 787 268 1524
Fragments arranged by size:
1524 888 787 597 410 393 268 203
198 181 45 31 25 22
EcoO109I rG'GnC_Cy
Cuts at: 2097 2412 2710 2772 2097
Size: 315 298 62 4897
Fragments arranged by size:
4897 315 298 62
EcoRI G'AATT_C
Cuts at: 2881 3259 2881
Size: 378 5194
FspI TGC'GCA
Cuts at: 4078 4078
Size: 5572
HaeII r_GCGC'y
Cuts at: 1678 4719 1678
Size: 3041 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 2520 2537 2552
Size: 32 135 32 857 32 59 17 15
Cuts at: 2552 2569 4213 4245 4287 4319 1373
Size: 17 1644 32 42 32 2626
Fragments arranged by size:
2626 1644 857 135 59 42 32 32
32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2916 3162 669
Size: 988 281 978 246 3079
Fragments arranged by size:
3079 988 978 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5572
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KpnI G_GTAC'C
Cuts at: 2897 2897
Size: 5572
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4686 4870 1305
Size: 3381 184 2007
Fragments arranged by size:
3381 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3750
Fragments arranged by size:
3750 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 2657 3530 3889 4048 518
Size: 648 1110 381 873 359 159 2042
Fragments arranged by size:
2042 1110 873 648 381 359 159
MspA1I CmG'CkG
Cuts at: 1606 2363 2374 2511 2706 2757 2839 2988 3665
Size: 757 11 137 195 51 82 149 677
Cuts at: 3665 4813 5058 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 757 677 245 195 149 137
82 51 11
NaeI GCC'GGC
Cuts at: 2808 2808
Size: 5572
NcoI C'CATG_G
Cuts at: 513 1065 2292 513
Size: 552 1227 3793
Fragments arranged by size:
3793 1227 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NgoMIV G'CCGG_C
Cuts at: 2806 2806
Size: 5572
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5572
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2930 4475 1132
Size: 97 1701 1545 2229
Fragments arranged by size:
2229 1701 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2086 2684 2844 1348
Size: 738 598 160 4076
Fragments arranged by size:
4076 738 598 160
PciI A'CATG_T
Cuts at: 4471 4471
Size: 5572
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2135 2263 1108
Size: 1027 128 4417
Fragments arranged by size:
4417 1027 128
PfoI T'CCnGG_A
Cuts at: 2323 2323
Size: 5572
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2412 2412
Size: 5572
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 3142 3142
Size: 5572
PstI C_TGCA'G
Cuts at: 830 2924 830
Size: 2094 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
PvuII CAG'CTG
Cuts at: 2374 2374
Size: 5572
SacII CC_GC'GG
Cuts at: 2364 2707 2364
Size: 343 5229
SalI G'TCGA_C
Cuts at: 1936 2914 1936
Size: 978 4594
SanDI GG'GwC_CC
Cuts at: 2412 2412
Size: 5572
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 3820 5532 3820
Size: 1712 3860
SfcI C'TryA_G
Cuts at: 826 1045 2920 4055 4736 4927 826
Size: 219 1875 1135 681 191 1471
Fragments arranged by size:
1875 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 2806 1700
Size: 1106 4466
SmaI CCC'GGG
Cuts at: 1872 2899 1872
Size: 1027 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3676 4577 4839 820
Size: 197 926 1733 901 262 1553
Fragments arranged by size:
1733 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2930 1132
Size: 1798 3774
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 2051 1517
Size: 534 5038
StyI C'CwwG_G
Cuts at: 513 1065 2112 2292 2565 2595 3011 513
Size: 552 1047 180 273 30 416 3074
Fragments arranged by size:
3074 1047 552 416 273 180 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2739 2836 3599 3616 3769 3954 4399
Size: 1255 1159 97 763 17 153 185 445
Cuts at: 4399 325
Size: 1498
Fragments arranged by size:
1498 1255 1159 763 445 185 153 97
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3818 5530 96
Size: 274 80 33 51 1443 1841 1712 138
Fragments arranged by size:
1841 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2714 2746 5532 5564 2714
Size: 32 2786 32 2722
Fragments arranged by size:
2786 2722 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5572
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2119
Size: 5572
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5572
XmaI C'CCGG_G
Cuts at: 1870 2897 1870
Size: 1027 4545
XmnI GAAnn'nnTTC
Cuts at: 3701 3701
Size: 5572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3380 276
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SapI SexAI SfiI SfoI SrfI SwaI