Restriction map for
pFN21AE0564
(Circular) (Six-base) MAPSORT of: pFN21AE0564.seq Check: 3921 from: 1 to: 6049
pFN21AE0564 6049 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3159 3859 278
Size: 53 83 186 936 1623 700 2468
Fragments arranged by size:
2468 1623 936 700 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3392 1937
Size: 1455 4594
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 3084 4176 4549 1563
Size: 1521 1092 373 3063
Fragments arranged by size:
3063 1521 1092 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2530 2693 2903 4112 5496 5764 1632
Size: 898 163 210 1209 1384 268 1917
Fragments arranged by size:
1917 1384 1209 898 268 210 163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 3186 4948 5925 1203
Size: 1983 1762 977 1327
Fragments arranged by size:
1983 1762 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 2434 2957 4778 2434
Size: 523 1821 3705
Fragments arranged by size:
3705 1821 523
AleI CACnn'nnGTG
Cuts at: 1166 3263 1166
Size: 2097 3952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2382 1184
Size: 1198 4851
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 6017
AlwNI CAG_nnn'CTG
Cuts at: 2214 3148 3792 5364 2214
Size: 934 644 1572 2899
Fragments arranged by size:
2899 1572 934 644
ApaI G_GGCC'C
Cuts at: 2252 3166 2252
Size: 914 5135
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 3358 3584 3736 5845 1493
Size: 1865 226 152 2109 1697
Fragments arranged by size:
2109 1865 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 3374 1870
Size: 73 1431 4545
Fragments arranged by size:
4545 1431 73
BamHI G'GATC_C
Cuts at: 2385 2616 3379 3746 2385
Size: 231 763 367 4688
Fragments arranged by size:
4688 763 367 231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3370 4825 618
Size: 325 217 627 1583 1455 1842
Fragments arranged by size:
1842 1583 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2252 2463 3166 3368 1391
Size: 861 211 703 202 4072
Fragments arranged by size:
4072 861 703 211 202
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5078
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4240 4274 1903
Size: 34 2303 34 3678
Fragments arranged by size:
3678 2303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 3075 1548
Size: 1527 4522
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2531 3098 3339
Size: 107 122 71 631 605 859 567 241
Cuts at: 3339 4660 136
Size: 1321 1525
Fragments arranged by size:
1525 1321 859 631 605 567 241 122
107 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 3466 3466
Size: 6049
Bme1580I G_kGCm'C
Cuts at: 1790 2252 3166 3270 3285 4110 5266 1790
Size: 462 914 104 15 825 1156 2573
Fragments arranged by size:
2573 1156 914 825 462 104 15
BmgBI CAC'GTC
Cuts at: 1318 2851 1318
Size: 1533 4516
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3780 4738 442
Size: 416 53 83 399 24 2363 958 1753
Fragments arranged by size:
2363 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 2396 2447 2672 2819 2990 3140 3191
Size: 1136 21 51 225 147 171 150 51
Cuts at: 3191 3224 4709 1239
Size: 33 1485 2579
Fragments arranged by size:
2579 1485 1136 225 171 150 147 51
51 33 21
Bpu10I CC'TnA_GC
Cuts at: 2103 2103
Size: 6049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 4174 5039 5337 2033
Size: 2141 865 298 2745
Fragments arranged by size:
2745 2141 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4712 882
Size: 617 3213 2219
Fragments arranged by size:
3213 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3189 3840 493
Size: 711 1985 651 2702
Fragments arranged by size:
2702 1985 711 651
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3156 3856 4238 4873
Size: 53 83 186 936 1623 700 382 635
Cuts at: 4873 275
Size: 1451
Fragments arranged by size:
1623 1451 936 700 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3192 4482 4880 5154 5301 5712 3192
Size: 1290 398 274 147 411 3529
Fragments arranged by size:
3529 1290 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2407 2437 2672 2702 2795
Size: 30 165 30 1004 30 235 30 93
Cuts at: 2795 2825 2846 2876 3224 3254 1178
Size: 30 21 30 348 30 3973
Fragments arranged by size:
3973 1004 348 235 165 93 30 30
30 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 2693 2723 2816 2894 1186
Size: 1285 222 30 93 78 4341
Fragments arranged by size:
4341 1285 222 93 78 30
BseYI C'CCAG_C
Cuts at: 2988 3051 3093 5252 5748 2988
Size: 63 42 2159 496 3289
Fragments arranged by size:
3289 2159 496 63 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2754 3238 1163
Size: 573 1018 484 3974
Fragments arranged by size:
3974 1018 573 484
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2320 3499 4260 5288 1540
Size: 275 186 319 1179 761 1028 2301
Fragments arranged by size:
2301 1179 1028 761 319 275 186
BsiHKAI G_wGCw'C
Cuts at: 2415 2463 4110 4195 5266 2415
Size: 48 1647 85 1071 3198
Fragments arranged by size:
3198 1647 1071 85 48
BsmI GAATG_Cn'
Cuts at: 2371 2500 3560 3653 2371
Size: 129 1060 93 4767
Fragments arranged by size:
4767 1060 129 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2944 2944
Size: 6049
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2252 2415 2463 3166 3270 3285 3368
Size: 399 462 163 48 703 104 15 83
Cuts at: 3368 4110 4195 5266 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 703 462 399 163 104
85 83 48 15
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3404 844
Size: 2560 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3124 3936 1394
Size: 429 1301 812 3507
Fragments arranged by size:
3507 1301 812 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2293 4544 4718 65
Size: 1183 1045 2251 174 1396
Fragments arranged by size:
2251 1396 1183 1045 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 4693 4880 1700
Size: 110 287 2596 187 2869
Fragments arranged by size:
2869 2596 287 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 4109 5121 4109
Size: 1012 5037
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2495 3321 794
Size: 1701 826 3522
Fragments arranged by size:
3522 1701 826
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6049
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 2797
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 2385 2616 3105 3379 3746 4131 4148 5600
Size: 413 231 489 274 367 385 17 1452
Cuts at: 5600 5686 5698 6044 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 489 413 385 367 346 274
231 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2999 2999
Size: 6049
BtgI C'CryG_G
Cuts at: 513 1065 2133 2244 2826 513
Size: 552 1068 111 582 3736
Fragments arranged by size:
3736 1068 582 552 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 3074 3565 4359 4379 3074
Size: 491 794 20 4744
Fragments arranged by size:
4744 794 491 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DraIII CAC_nnn'GTG
Cuts at: 2637 2637
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2174 2325 2571
Size: 54 1425 50 275 18 344 151 246
Cuts at: 2571 2898 3496 4385 6014 8
Size: 327 598 889 1629 43
Fragments arranged by size:
1629 1425 889 598 344 327 275 246
151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3979 1353
Size: 2626 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2461 2461
Size: 6049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2375 2396 2447 2530 2672 2693 2819
Size: 393 743 21 51 83 142 21 126
Cuts at: 2819 2903 2990 3140 3191 3224 4112 4709 5496
Size: 84 87 150 51 33 888 597 787
Cuts at: 5496 5764 1239
Size: 268 1524
Fragments arranged by size:
1524 888 787 743 597 393 268 150
142 126 87 84 83 51 51 33
21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2675 2675
Size: 6049
EcoO109I rG'GnC_Cy
Cuts at: 2249 2274 2249
Size: 25 6024
EcoRI G'AATT_C
Cuts at: 3358 3736 3358
Size: 378 5671
FspI TGC'GCA
Cuts at: 2612 4555 2612
Size: 1943 4106
HaeII r_GCGC'y
Cuts at: 1678 5196 1678
Size: 3518 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 2797 2829 2943
Size: 32 135 32 848 32 345 32 114
Cuts at: 2943 2975 4690 4722 4764 4796 1373
Size: 32 1715 32 42 32 2626
Fragments arranged by size:
2626 1715 848 345 135 114 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2962 3393 3639 669
Size: 988 281 1024 431 246 3079
Fragments arranged by size:
3079 1024 988 431 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5163 5347 1305
Size: 3858 184 2007
Fragments arranged by size:
3858 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 2573 2900 10
Size: 54 1425 343 495 246 327 3159
Fragments arranged by size:
3159 1425 495 343 327 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2759 3263 4007 4366 4525 518
Size: 648 1593 504 744 359 159 2042
Fragments arranged by size:
2042 1593 744 648 504 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2184 2259 2353 2584 3055 3097 3465
Size: 479 99 75 94 231 471 42 368
Cuts at: 3465 4142 5290 5535 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 479 471 368 245 231
99 94 75 42
NaeI GCC'GGC
Cuts at: 2099 2099
Size: 6049
NcoI C'CATG_G
Cuts at: 513 1065 2133 2244 2826 513
Size: 552 1068 111 582 3736
Fragments arranged by size:
3736 1068 582 552 111
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NgoMIV G'CCGG_C
Cuts at: 2097 2097
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2142 3319 3407 4952 1132
Size: 97 913 1177 88 1545 2229
Fragments arranged by size:
2229 1545 1177 913 97 88
PasI CC'CwG_GG
Cuts at: 1348 3337 1348
Size: 1989 4060
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2134 2896 1108
Size: 1026 762 4261
Fragments arranged by size:
4261 1026 762
PfoI T'CCnGG_A
Cuts at: 2619 2619
Size: 6049
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 4118 4150 5631 5663 2846
Size: 32 1240 32 1481 32 3232
Fragments arranged by size:
3232 1481 1240 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PspOMI G'GGCC_C
Cuts at: 2248 3162 2248
Size: 914 5135
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 6017
PstI C_TGCA'G
Cuts at: 830 2985 3401 830
Size: 2155 416 3478
Fragments arranged by size:
3478 2155 416
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
PvuII CAG'CTG
Cuts at: 2184 2259 2353 2584 3055 3097 2184
Size: 75 94 231 471 42 5136
Fragments arranged by size:
5136 471 231 94 75 42
SacI G_AGCT'C
Cuts at: 2463 2463
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SexAI A'CCwGG_T
Cuts at: 3141 3141
Size: 6049
SfcI C'TryA_G
Cuts at: 826 1045 2981 3043 3397 4532 5213 5404 826
Size: 219 1936 62 354 1135 681 191 1471
Fragments arranged by size:
1936 1471 1135 681 354 219 191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 4153 5054 5316 820
Size: 197 926 105 2105 901 262 1553
Fragments arranged by size:
2105 1553 926 901 262 197 105
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 2142 3319 3407 1132
Size: 1010 1177 88 3774
Fragments arranged by size:
3774 1177 1010 88
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 2396 1517
Size: 879 5170
StyI C'CwwG_G
Cuts at: 513 1065 2133 2244 2484 2826 3488 513
Size: 552 1068 111 240 342 662 3074
Fragments arranged by size:
3074 1068 662 552 342 240 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2062 2140 4076 4093 4246 4431 4876
Size: 1255 482 78 1936 17 153 185 445
Cuts at: 4876 325
Size: 1498
Fragments arranged by size:
1936 1498 1255 482 445 185 153 78
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4295 6007 96
Size: 274 80 33 51 1443 2318 1712 138
Fragments arranged by size:
2318 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2407 2439 2672 2704 2795 2827 6009 6041 2407
Size: 32 233 32 91 32 3182 32 2415
Fragments arranged by size:
3182 2415 233 91 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2225 2581 2225
Size: 356 5693
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3157 3857 276
Size: 53 83 186 936 1623 700 2468
Fragments arranged by size:
2468 1623 936 700 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PspXI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI