Restriction map for
pFN21AE0562
(Circular) (Six-base) MAPSORT of: pFN21AE0562.seq Check: 5238 from: 1 to: 5689
pFN21AE0562 5689 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 3032 1937
Size: 169 926 4594
Fragments arranged by size:
4594 926 169
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 3752 5136 5404 1632
Size: 653 1467 1384 268 1917
Fragments arranged by size:
1917 1467 1384 653 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 2041 2692 3432 5004 2041
Size: 651 740 1572 2726
Fragments arranged by size:
2726 1572 740 651
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2336 3010 4465 618
Size: 325 217 627 549 674 1455 1842
Fragments arranged by size:
1842 1455 674 627 549 325 217
BanII G_rGCy'C
Cuts at: 1391 2641 2805 3008 1391
Size: 1250 164 203 4072
Fragments arranged by size:
4072 1250 203 164
BbeI G_GCGC'C
Cuts at: 2340 2340
Size: 5689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2180 2273 2384 2758 928
Size: 971 281 93 111 374 3859
Fragments arranged by size:
3859 971 374 281 111 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2593 2627 3880 3914 1903
Size: 34 656 34 1253 34 3678
Fragments arranged by size:
3678 1253 656 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 2683 1548
Size: 1135 4554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 2504 3106 2504
Size: 602 5087
Bme1580I G_kGCm'C
Cuts at: 1790 2574 3750 4906 1790
Size: 784 1176 1156 2573
Fragments arranged by size:
2573 1176 1156 784
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2808 3420 4378
Size: 416 53 83 399 24 1391 612 958
Cuts at: 4378 442
Size: 1753
Fragments arranged by size:
1753 1391 958 612 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 2612 2800 4349 1239
Size: 1340 33 188 1549 2579
Fragments arranged by size:
2579 1549 1340 188 33
Bpu10I CC'TnA_GC
Cuts at: 2286 2286
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2960 4352 882
Size: 617 1461 1392 2219
Fragments arranged by size:
2219 1461 1392 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2337 3496 3878 4513
Size: 53 83 186 936 804 1159 382 635
Cuts at: 4513 275
Size: 1451
Fragments arranged by size:
1451 1159 936 804 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2388 1186
Size: 1202 4487
BseYI C'CCAG_C
Cuts at: 4892 5388 4892
Size: 496 5193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2338 2906 1163
Size: 573 602 568 3946
Fragments arranged by size:
3946 602 573 568
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2292 2474 3750 3835 4906 2292
Size: 182 1276 85 1071 3075
Fragments arranged by size:
3075 1276 1071 182 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2474 2574 2641 2805 3008 3750
Size: 399 502 182 100 67 164 203 742
Cuts at: 3750 3835 4906 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 399 203 182 164
100 85 67
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 3044 844
Size: 1274 926 3489
Fragments arranged by size:
3489 1274 926
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4333 4520 1700
Size: 110 2523 187 2869
Fragments arranged by size:
2869 2523 187 110
BsrGI T'GTAC_A
Cuts at: 96 2715 96
Size: 2619 3070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 2525 3749 4761 2525
Size: 1224 1012 3453
Fragments arranged by size:
3453 1224 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2352 2569 794
Size: 1558 217 3914
Fragments arranged by size:
3914 1558 217
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstXI CCAn_nnnn'nTGG
Cuts at: 2516 2516
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2085 2430 3019 3386 3771 3788 5240 5326
Size: 113 345 589 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 589 385 367 346 345 113
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2317 2509 513
Size: 552 939 313 192 3693
Fragments arranged by size:
3693 939 552 313 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2060 2852 3205 3999 4019 2060
Size: 792 353 794 20 3730
Fragments arranged by size:
3730 794 792 353 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2129 2406 2457
Size: 54 1425 50 275 18 299 277 51
Cuts at: 2457 3136 4025 5654 8
Size: 679 889 1629 43
Fragments arranged by size:
1629 1425 889 679 299 277 275 54
51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3619 1353
Size: 2266 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2726 4276 4650 4796 387
Size: 1064 138 1137 1550 374 146 1280
Fragments arranged by size:
1550 1280 1137 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2579 2612 2800 3752 4349 5136
Size: 393 653 294 33 188 952 597 787
Cuts at: 5136 5404 1239
Size: 268 1524
Fragments arranged by size:
1524 952 787 653 597 393 294 268
188 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2209 2440 2209
Size: 231 5458
EcoO109I rG'GnC_Cy
Cuts at: 2275 2557 2275
Size: 282 5407
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 5689
FspI TGC'GCA
Cuts at: 2618 4195 2618
Size: 1577 4112
FspAI rTGC'GCAy
Cuts at: 2618 2618
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2340 4836 1678
Size: 662 2496 2531
Fragments arranged by size:
2531 2496 662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373
Size: 32 135 32 2758 32 42 32 2626
Fragments arranged by size:
2758 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2779 748
Size: 2031 3658
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KasI G'GCGC_C
Cuts at: 2336 2336
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 2459 10
Size: 54 1425 343 576 51 3240
Fragments arranged by size:
3240 1425 576 343 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2125 2316 2537 3647 4006 4165 518
Size: 648 959 191 221 1110 359 159 2042
Fragments arranged by size:
2042 1110 959 648 359 221 191 159
MspA1I CmG'CkG
Cuts at: 1606 3105 3782 4930 5175 1606
Size: 1499 677 1148 245 2120
Fragments arranged by size:
2120 1499 1148 677 245
NarI GG'CG_CC
Cuts at: 2337 2337
Size: 5689
NcoI C'CATG_G
Cuts at: 513 1065 2004 2317 2509 513
Size: 552 939 313 192 3693
Fragments arranged by size:
3693 939 552 313 192
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2082 2884 1108
Size: 974 802 3913
Fragments arranged by size:
3913 974 802
PfoI T'CCnGG_A
Cuts at: 2163 2163
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2275
Size: 5689
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2055 3041 830
Size: 1225 986 3478
Fragments arranged by size:
3478 1225 986
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2051 3037 4172 4853 5044 826
Size: 219 1006 986 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 986 681 219 191
SfoI GGC'GCC
Cuts at: 2338 2338
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3793 4694 4956 820
Size: 197 926 1850 901 262 1553
Fragments arranged by size:
1850 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 2821 1517
Size: 1304 4385
StyI C'CwwG_G
Cuts at: 513 1065 2004 2317 2509 3128 513
Size: 552 939 313 192 619 3074
Fragments arranged by size:
3074 939 619 552 313 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2540 3716 3733 3886 4071 4516 325
Size: 1255 960 1176 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1176 960 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2715 3935 5647
Size: 274 80 33 51 1443 738 1220 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1443 1220 738 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2206 2238 5649 5681 2206
Size: 32 3411 32 2214
Fragments arranged by size:
3411 2214 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 2381 3818 2381
Size: 1437 4252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI