Restriction map for
pFN21AE0559
(Circular) (Six-base) MAPSORT of: pFN21AE0559.seq Check: 2625 from: 1 to: 5590
pFN21AE0559 5590 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2337 2337
Size: 5590
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3400 278
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2933 1937
Size: 996 4594
Acc65I G'GTAC_C
Cuts at: 2520 2911 2520
Size: 391 5199
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3653 5037 5305 1632
Size: 2021 1384 268 1917
Fragments arranged by size:
2021 1917 1384 268
AfeI AGC'GCT
Cuts at: 2828 2828
Size: 5590
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 2682 4489 5466 1203
Size: 1479 1807 977 1327
Fragments arranged by size:
1807 1479 1327 977
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 2162 1166
Size: 996 4594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AlwNI CAG_nnn'CTG
Cuts at: 2176 2356 3333 4905 2176
Size: 180 977 1572 2861
Fragments arranged by size:
2861 1572 977 180
ApaI G_GGCC'C
Cuts at: 2094 2094
Size: 5590
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2915 1870
Size: 73 972 4545
Fragments arranged by size:
4545 972 73
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 2239 2520 2911 4366
Size: 325 217 627 366 86 281 391 1455
Cuts at: 4366 618
Size: 1842
Fragments arranged by size:
1842 1455 627 391 366 325 281 217
86
BanII G_rGCy'C
Cuts at: 1391 2094 2224 2745 2909 1391
Size: 703 130 521 164 4072
Fragments arranged by size:
4072 703 521 164 130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2592 2804 928
Size: 971 693 212 3714
Fragments arranged by size:
3714 971 693 212
BbvCI CC'TCA_GC
Cuts at: 2870 2870
Size: 5590
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2311 4201 136
Size: 107 122 71 631 605 639 1890 1525
Fragments arranged by size:
1890 1525 639 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5585 5585
Size: 5590
BlpI GC'TnA_GC
Cuts at: 2549 3007 2549
Size: 458 5132
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2244 3651 4807 1790
Size: 304 150 1407 1156 2573
Fragments arranged by size:
2573 1407 1156 304 150
BmgBI CAC'GTC
Cuts at: 1318 2407 2685 1318
Size: 1089 278 4223
Fragments arranged by size:
4223 1089 278
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2096 3321 4279
Size: 416 53 83 399 24 679 1225 958
Cuts at: 4279 442
Size: 1753
Fragments arranged by size:
1753 1225 958 679 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2303 2517 2648 4250 1239
Size: 821 243 214 131 1602 2579
Fragments arranged by size:
2579 1602 821 243 214 131
Bpu10I CC'TnA_GC
Cuts at: 2454 2870 2454
Size: 416 5174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2270 3715 4580 4878 2270
Size: 1445 865 298 2982
Fragments arranged by size:
2982 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2419 2651 4253 882
Size: 617 920 232 1602 2219
Fragments arranged by size:
2219 1602 920 617 232
BsaAI yAC'GTr
Cuts at: 493 1204 2047 2233 2530 3381 493
Size: 711 843 186 297 851 2702
Fragments arranged by size:
2702 851 843 711 297 186
BsaBI GATnn'nnATC
Cuts at: 5584 5584
Size: 5590
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3397 3779 4414 275
Size: 53 83 186 936 1864 382 635 1451
Fragments arranged by size:
1864 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2744 2774 1178
Size: 30 165 30 1341 30 3994
Fragments arranged by size:
3994 1341 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2669 1186
Size: 1483 4107
BseYI C'CCAG_C
Cuts at: 2786 4793 5289 2786
Size: 2007 496 3087
Fragments arranged by size:
3087 2007 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 4201
Fragments arranged by size:
4201 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 3801 4829 1540
Size: 275 186 1039 761 1028 2301
Fragments arranged by size:
2301 1039 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2745 3651 3736 4807 2745
Size: 906 85 1071 3528
Fragments arranged by size:
3528 1071 906 85
BsiWI C'GTAC_G
Cuts at: 2233 2233
Size: 5590
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2224 2244 2745 2909 3651 3736
Size: 399 304 130 20 501 164 742 85
Cuts at: 3736 4807 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 501 399 304 164 130
85 20
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 2769 3479 5209 2769
Size: 710 1730 3150
Fragments arranged by size:
3150 1730 710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2337 2945 844
Size: 1289 204 608 3489
Fragments arranged by size:
3489 1289 608 204
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 2880 3477 1394
Size: 429 239 818 597 3507
Fragments arranged by size:
3507 818 597 429 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 4085 4259 65
Size: 1183 2837 174 1396
Fragments arranged by size:
2837 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4234 4421 1700
Size: 110 2424 187 2869
Fragments arranged by size:
2869 2424 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 3650 4662 3650
Size: 1012 4578
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5590
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5590
BstYI r'GATC_y
Cuts at: 1972 2622 2920 3287 3672 3689 5141 5227 5239
Size: 650 298 367 385 17 1452 86 12
Cuts at: 5239 5585 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 650 385 367 346 298 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2275 2275
Size: 5590
BtgI C'CryG_G
Cuts at: 513 1065 2004 2309 2661 2811 513
Size: 552 939 305 352 150 3292
Fragments arranged by size:
3292 939 552 352 305 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4851
Fragments arranged by size:
4851 683 56
BtsI GCAGTG_nn'
Cuts at: 3106 3900 3920 3106
Size: 794 20 4776
Fragments arranged by size:
4776 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2388 2423 529
Size: 35 1824 35 3696
Fragments arranged by size:
3696 1824 35 35
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2411 4597 809
Size: 507 1095 2186 1802
Fragments arranged by size:
2186 1802 1095 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3037 3926 5555
Size: 54 1425 50 275 18 1207 889 1629
Cuts at: 5555 8
Size: 43
Fragments arranged by size:
1629 1425 1207 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2582 3520 1353
Size: 1229 938 3423
Fragments arranged by size:
3423 1229 938
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2743 2743
Size: 5590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2303 2517 2648 3653 4250 5037
Size: 393 428 243 214 131 1005 597 787
Cuts at: 5037 5305 1239
Size: 268 1524
Fragments arranged by size:
1524 1005 787 597 428 393 268 243
214 131
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2770 2651
Size: 119 5471
EcoO109I rG'GnC_Cy
Cuts at: 2056 2090 2091 2463 2514 2056
Size: 34 1 372 51 5132
Fragments arranged by size:
5132 372 51 34 1
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 5590
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 2644 2830 4737 1678
Size: 966 186 1907 2531
Fragments arranged by size:
2531 1907 966 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373
Size: 32 135 32 2659 32 42 32 2626
Fragments arranged by size:
2659 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KpnI G_GTAC'C
Cuts at: 2524 2915 2524
Size: 391 5199
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2340 4704 4888 1305
Size: 1035 2364 184 2007
Fragments arranged by size:
2364 2007 1035 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3768
Fragments arranged by size:
3768 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 3548 3907 4066 518
Size: 648 996 1386 359 159 2042
Fragments arranged by size:
2042 1386 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3006 3683 4831 5076 1606
Size: 1400 677 1148 245 2120
Fragments arranged by size:
2120 1400 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2661 513
Size: 552 939 657 3442
Fragments arranged by size:
3442 939 657 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NspI r_CATG'y
Cuts at: 1132 1229 2948 4493 1132
Size: 97 1719 1545 2229
Fragments arranged by size:
2229 1719 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5590
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 2437 1108
Size: 1068 261 4261
Fragments arranged by size:
4261 1068 261
PfoI T'CCnGG_A
Cuts at: 2016 2478 2625 2016
Size: 462 147 4981
Fragments arranged by size:
4981 462 147
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PmlI CAC'GTG
Cuts at: 2530 2530
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2463 2514 2056
Size: 407 51 5132
Fragments arranged by size:
5132 407 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 3160 3160
Size: 5590
PspOMI G'GGCC_C
Cuts at: 2090 2090
Size: 5590
PstI C_TGCA'G
Cuts at: 830 2100 2867 2942 830
Size: 1270 767 75 3478
Fragments arranged by size:
3478 1270 767 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
SacI G_AGCT'C
Cuts at: 2745 2745
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SanDI GG'GwC_CC
Cuts at: 2056 2056
Size: 5590
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 2072 3838 5550 2072
Size: 1766 1712 2112
Fragments arranged by size:
2112 1766 1712
SfcI C'TryA_G
Cuts at: 826 1045 2096 2863 2938 4073 4754 4945 826
Size: 219 1051 767 75 1135 681 191 1471
Fragments arranged by size:
1471 1135 1051 767 681 219 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 3694 4595 4857 820
Size: 197 926 306 1445 901 262 1553
Fragments arranged by size:
1553 1445 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 2233 3381 493
Size: 1740 1148 2702
Fragments arranged by size:
2702 1740 1148
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2948 1132
Size: 1816 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5590
StyI C'CwwG_G
Cuts at: 513 1065 2004 2661 3029 513
Size: 552 939 657 368 3074
Fragments arranged by size:
3074 939 657 552 368
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2152 2573 3617 3634 3787 3972 4417
Size: 1255 572 421 1044 17 153 185 445
Cuts at: 4417 325
Size: 1498
Fragments arranged by size:
1498 1255 1044 572 445 421 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2070 3836 5548
Size: 274 80 33 51 1443 93 1766 1712
Cuts at: 5548 96
Size: 138
Fragments arranged by size:
1766 1712 1443 274 138 93 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 5550 5582 2744
Size: 32 2774 32 2752
Fragments arranged by size:
2774 2752 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2668 2809 2668
Size: 141 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3398 276
Size: 53 83 186 936 1864 2468
Fragments arranged by size:
2468 1864 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsmBI
BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI PvuII
RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI