Restriction map for
pFN21AE0555
(Circular) (Six-base) MAPSORT of: pFN21AE0555.seq Check: 7108 from: 1 to: 5836
pFN21AE0555 5836 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2808 3179 1937
Size: 871 371 4594
Fragments arranged by size:
4594 871 371
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 2520 2864 3899 5283 5551 1632
Size: 818 70 344 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1035 818 344 268 70
AfeI AGC'GCT
Cuts at: 2096 2120 2096
Size: 24 5812
AflII C'TTAA_G
Cuts at: 820 1017 2419 820
Size: 197 1402 4237
Fragments arranged by size:
4237 1402 197
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2332 2364 2332
Size: 32 5804
AlwNI CAG_nnn'CTG
Cuts at: 2498 3579 5151 2498
Size: 1081 1572 3183
Fragments arranged by size:
3183 1572 1081
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2856 3012 3145 3371 3523 5632 1493
Size: 1363 156 133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1363 226 156 152 133
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 3161 1870
Size: 73 189 1029 4545
Fragments arranged by size:
4545 1029 189 73
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3157 4612 618
Size: 325 217 627 1370 1455 1842
Fragments arranged by size:
1842 1455 1370 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2464 3155 1391
Size: 1073 691 4072
Fragments arranged by size:
4072 1073 691
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2265 2797 928
Size: 971 366 532 3967
Fragments arranged by size:
3967 971 532 366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3728 4938 3728
Size: 1210 4626
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BfrBI ATG'CAT
Cuts at: 2969 2969
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4447 136
Size: 107 122 71 631 605 337 2438 1525
Fragments arranged by size:
2438 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3070 5831 3070
Size: 2761 3075
BlpI GC'TnA_GC
Cuts at: 2446 3253 2446
Size: 807 5029
Bme1580I G_kGCm'C
Cuts at: 1790 3897 5053 1790
Size: 2107 1156 2573
Fragments arranged by size:
2573 2107 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3567 4525 442
Size: 416 53 83 399 24 2150 958 1753
Fragments arranged by size:
2150 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2033 1056
Size: 977 4859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 4496 1239
Size: 848 2409 2579
Fragments arranged by size:
2579 2409 848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2476 2892 3961 4826 5124 2476
Size: 416 1069 865 298 3188
Fragments arranged by size:
3188 1069 865 416 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4499 882
Size: 617 3000 2219
Fragments arranged by size:
3000 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2080 3627 493
Size: 711 876 1547 2702
Fragments arranged by size:
2702 1547 876 711
BsaBI GATnn'nnATC
Cuts at: 3075 5830 3075
Size: 2755 3081
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3643 4025 4660 275
Size: 53 83 186 936 2110 382 635 1451
Fragments arranged by size:
2110 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2473 2503 1178
Size: 30 165 30 1070 30 4511
Fragments arranged by size:
4511 1070 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5836
BseYI C'CCAG_C
Cuts at: 5039 5535 5039
Size: 496 5340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2511 1163
Size: 573 775 4488
Fragments arranged by size:
4488 775 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3897 3982 5053 3897
Size: 85 1071 4680
Fragments arranged by size:
4680 1071 85
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
BsmBI CGTCTCn'nnnn_
Cuts at: 2406 2406
Size: 5836
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2464 3155 3897 3982 5053 1391
Size: 399 674 691 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 691 674 399 85
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2233 3191 844
Size: 1289 100 958 3489
Fragments arranged by size:
3489 1289 958 100
BsrBI CCG'CTC
Cuts at: 1394 1823 2040 2053 3723 1394
Size: 429 217 13 1670 3507
Fragments arranged by size:
3507 1670 429 217 13
BsrDI GCAATG_nn'
Cuts at: 65 1248 2367 2379 2682 4331 4505 65
Size: 1183 1119 12 303 1649 174 1396
Fragments arranged by size:
1649 1396 1183 1119 303 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 4480 4667 1700
Size: 110 2670 187 2869
Fragments arranged by size:
2869 2670 187 110
BsrGI T'GTAC_A
Cuts at: 96 3121 96
Size: 3025 2811
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2162 794
Size: 1368 4468
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 2979 3070 3166 3533 3918 3935 5387 5473
Size: 1007 91 96 367 385 17 1452 86
Cuts at: 5473 5485 5831 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1007 385 367 346 96 91
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 2512 3352 4146 4166 2512
Size: 840 794 20 4182
Fragments arranged by size:
4182 840 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2362 2939
Size: 54 1425 50 275 18 180 352 577
Cuts at: 2939 3283 4172 5801 8
Size: 344 889 1629 43
Fragments arranged by size:
1629 1425 889 577 352 344 275 180
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2302 3766 1353
Size: 949 1464 3423
Fragments arranged by size:
3423 1464 949
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2450 2520 2864 3899 4496 5283
Size: 393 455 363 70 344 1035 597 787
Cuts at: 5283 5551 1239
Size: 268 1524
Fragments arranged by size:
1524 1035 787 597 455 393 363 344
268 70
EcoO109I rG'GnC_Cy
Cuts at: 2245 2245
Size: 5836
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2933 2933
Size: 5836
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 2098 2122 4983 1678
Size: 420 24 2861 2531
Fragments arranged by size:
2861 2531 420 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373
Size: 32 135 32 2905 32 42 32 2626
Fragments arranged by size:
2905 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2148 3180 3426 669
Size: 988 281 210 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246 210
HindIII A'AGCT_T
Cuts at: 748 2439 2846 748
Size: 1691 407 3738
Fragments arranged by size:
3738 1691 407
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 2151 3435 2151
Size: 1284 4552
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 4950 5134 1305
Size: 861 2784 184 2007
Fragments arranged by size:
2784 2007 861 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2364 2941 10
Size: 54 1425 343 532 577 2905
Fragments arranged by size:
2905 1425 577 532 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2352 2372 2579 2955 3794 4153 4312
Size: 648 1186 20 207 376 839 359 159
Cuts at: 4312 518
Size: 2042
Fragments arranged by size:
2042 1186 839 648 376 359 207 159
20
MspA1I CmG'CkG
Cuts at: 1606 2470 3252 3929 5077 5322 1606
Size: 864 782 677 1148 245 2120
Fragments arranged by size:
2120 1148 864 782 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 2029 1052
Size: 977 4859
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NsiI A_TGCA'T
Cuts at: 2971 2971
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 2521 2969 3194 4739 1132
Size: 97 1292 448 225 1545 2229
Fragments arranged by size:
2229 1545 1292 448 225 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5836
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PfoI T'CCnGG_A
Cuts at: 2335 2335
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PmlI CAC'GTG
Cuts at: 2080 2080
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2245 2245
Size: 5836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 2244 3188 830
Size: 1414 944 3478
Fragments arranged by size:
3478 1414 944
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2470 2470
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SapI GCTCTTCn'nnn_
Cuts at: 2302 2302
Size: 5836
SbfI CC_TGCA'GG
Cuts at: 2244 3188 2244
Size: 944 4892
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 2240 2805 3184 4319 5000 5191 826
Size: 219 1195 565 379 1135 681 191 1471
Fragments arranged by size:
1471 1195 1135 681 565 379 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2419 2455 2907 3940 4841
Size: 197 926 189 287 36 452 1033 901
Cuts at: 4841 5103 820
Size: 262 1553
Fragments arranged by size:
1553 1033 926 901 452 287 262 197
189 36
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 2969 3194 1132
Size: 1837 225 3774
Fragments arranged by size:
3774 1837 225
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2004 3093 3275 513
Size: 552 939 1089 182 3074
Fragments arranged by size:
3074 1089 939 552 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2818 3863 3880 4033 4218 4663 325
Size: 1255 1238 1045 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1238 1045 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2529 3121 4082
Size: 274 80 33 51 1443 552 592 961
Cuts at: 4082 5794 96
Size: 1712 138
Fragments arranged by size:
1712 1443 961 592 552 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2473 2505 5796 5828 2473
Size: 32 3291 32 2481
Fragments arranged by size:
3291 2481 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3067 3172 3067
Size: 105 5731
XhoI C'TCGA_G
Cuts at: 1943 2132 1943
Size: 189 5647
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 3063 3965 3063
Size: 902 4934
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI