Restriction map for
pFN21AE0554
(Circular) (Six-base) MAPSORT of: pFN21AE0554.seq Check: 8142 from: 1 to: 5425
pFN21AE0554 5425 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2262 2262
Size: 5425
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2746 2746
Size: 5425
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3488 4872 5140 1632
Size: 1856 1384 268 1917
Fragments arranged by size:
1917 1856 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 2479 4324 5301 1203
Size: 1276 1845 977 1327
Fragments arranged by size:
1845 1327 1276 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 2418 1166
Size: 1252 4173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2105 2513 1184
Size: 921 408 4096
Fragments arranged by size:
4096 921 408
AlwNI CAG_nnn'CTG
Cuts at: 2031 2311 2376 3168 4740 2031
Size: 280 65 792 1572 2716
Fragments arranged by size:
2716 1572 792 280 65
ApaI G_GGCC'C
Cuts at: 2233 2233
Size: 5425
ApaLI G'TGCA_C
Cuts at: 3482 4638 3482
Size: 1156 4269
ApoI r'AATT_y
Cuts at: 1493 2734 2960 3112 5221 1493
Size: 1241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2346 2750 1870
Size: 73 112 291 404 4545
Fragments arranged by size:
4545 404 291 112 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2746 4201 618
Size: 325 217 627 959 1455 1842
Fragments arranged by size:
1842 1455 959 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2233 2572 2744 1391
Size: 842 339 172 4072
Fragments arranged by size:
4072 842 339 172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2373 928
Size: 971 474 3980
Fragments arranged by size:
3980 971 474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3616 3650 1903
Size: 34 1679 34 3678
Fragments arranged by size:
3678 1679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2722 3317 4527 2722
Size: 595 1210 3620
Fragments arranged by size:
3620 1210 595
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2077 2684 4036
Size: 107 122 71 631 605 405 607 1352
Cuts at: 4036 136
Size: 1525
Fragments arranged by size:
1525 1352 631 607 605 405 122 107
71
BglII A'GATC_T
Cuts at: 5420 5420
Size: 5425
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2233 3486 4642 1790
Size: 443 1253 1156 2573
Fragments arranged by size:
2573 1253 1156 443
BmgBI CAC'GTC
Cuts at: 1318 2480 1318
Size: 1162 4263
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2566 2708 3156
Size: 416 53 83 399 24 1149 142 448
Cuts at: 3156 4114 442
Size: 958 1753
Fragments arranged by size:
1753 1149 958 448 416 399 142 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2118 2333 2354 4085 1239
Size: 879 215 21 1731 2579
Fragments arranged by size:
2579 1731 879 215 21
Bpu10I CC'TnA_GC
Cuts at: 2571 2571
Size: 5425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4088 882
Size: 617 2589 2219
Fragments arranged by size:
2589 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3216 493
Size: 711 2012 2702
Fragments arranged by size:
2702 2012 711
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3232 3614 4249 275
Size: 53 83 186 936 1699 382 635 1451
Fragments arranged by size:
1699 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3858 4256 4530 4677 5088 3858
Size: 398 274 147 411 4195
Fragments arranged by size:
4195 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2580 2610 1178
Size: 30 165 30 1177 30 3993
Fragments arranged by size:
3993 1177 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 2734 1186
Size: 1252 296 3877
Fragments arranged by size:
3877 1252 296
BseYI C'CCAG_C
Cuts at: 2199 2278 2301 2473 4628 5124 2199
Size: 79 23 172 2155 496 2500
Fragments arranged by size:
2500 2155 496 172 79 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2310 1163
Size: 573 574 4278
Fragments arranged by size:
4278 574 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2343 2875 3636 4664 1540
Size: 275 186 342 532 761 1028 2301
Fragments arranged by size:
2301 1028 761 532 342 275 186
BsiHKAI G_wGCw'C
Cuts at: 2572 3486 3571 4642 2572
Size: 914 85 1071 3355
Fragments arranged by size:
3355 1071 914 85
BsmI GAATG_Cn'
Cuts at: 2228 2936 3029 2228
Size: 708 93 4624
Fragments arranged by size:
4624 708 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2572 2744 3486 3571 4642 1391
Size: 399 443 339 172 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 339 172 85
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 2218 3314 5044 2218
Size: 1096 1730 2599
Fragments arranged by size:
2599 1730 1096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2102 2262 2780 844
Size: 1258 160 518 3489
Fragments arranged by size:
3489 1258 518 160
BsrBI CCG'CTC
Cuts at: 1394 1823 2671 3312 1394
Size: 429 848 641 3507
Fragments arranged by size:
3507 848 641 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2490 3920 4094 65
Size: 1183 1242 1430 174 1396
Fragments arranged by size:
1430 1396 1242 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2338 4069 4256 1700
Size: 110 528 1731 187 2869
Fragments arranged by size:
2869 1731 528 187 110
BsrGI T'GTAC_A
Cuts at: 96 2482 96
Size: 2386 3039
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5425
BssSI C'ACGA_G
Cuts at: 3485 4497 3485
Size: 1012 4413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5425
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2205 2755 3122 3507 3524 4976 5062 5074
Size: 233 550 367 385 17 1452 86 12
Cuts at: 5074 5420 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 550 385 367 346 233 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2534 2664 2534
Size: 130 5295
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4873
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2327 2689 2941 3735 3755 2327
Size: 362 252 794 20 3997
Fragments arranged by size:
3997 794 362 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2872 3761
Size: 54 1425 50 275 18 192 850 889
Cuts at: 3761 5390 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 850 275 192 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 1537
Size: 275 1060 4090
Fragments arranged by size:
4090 1060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3355 1353
Size: 2002 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4012 4386 4532 387
Size: 1064 138 2423 374 146 1280
Fragments arranged by size:
2423 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2570 2570
Size: 5425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2333 2354 3488 4085 4872 5140
Size: 393 486 215 21 1134 597 787 268
Cuts at: 5140 1239
Size: 1524
Fragments arranged by size:
1524 1134 787 597 486 393 268 215
21
EcoO109I rG'GnC_Cy
Cuts at: 2160 2229 2428 2676 2160
Size: 69 199 248 4909
Fragments arranged by size:
4909 248 199 69
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 2072 4572 1678
Size: 394 2500 2531
Fragments arranged by size:
2531 2500 394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2197 2229 2630
Size: 32 135 32 461 32 132 32 401
Cuts at: 2630 2662 4066 4098 4140 4172 1373
Size: 32 1404 32 42 32 2626
Fragments arranged by size:
2626 1404 461 401 135 132 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KpnI G_GTAC'C
Cuts at: 2750 2750
Size: 5425
MfeI C'AATT_G
Cuts at: 3024 3024
Size: 5425
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2332 4539 4723 1305
Size: 1027 2207 184 2007
Fragments arranged by size:
2207 2007 1027 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 10
Size: 54 1425 343 192 3411
Fragments arranged by size:
3411 1425 343 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2217 2418 2559 3383 3742 3901
Size: 648 843 208 201 141 824 359 159
Cuts at: 3901 518
Size: 2042
Fragments arranged by size:
2042 843 824 648 359 208 201 159
141
MspA1I CmG'CkG
Cuts at: 1606 2376 2418 2841 3518 4666 4911 1606
Size: 770 42 423 677 1148 245 2120
Fragments arranged by size:
2120 1148 770 677 423 245 42
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4873
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2783 4328 1132
Size: 97 1554 1545 2229
Fragments arranged by size:
2229 1554 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5425
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2391 1108
Size: 1283 4142
PfoI T'CCnGG_A
Cuts at: 2714 2714
Size: 5425
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2160 2160
Size: 5425
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5425
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PspOMI G'GGCC_C
Cuts at: 2229 2229
Size: 5425
PstI C_TGCA'G
Cuts at: 830 2520 2777 830
Size: 1690 257 3478
Fragments arranged by size:
3478 1690 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
SacI G_AGCT'C
Cuts at: 2572 2572
Size: 5425
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SanDI GG'GwC_CC
Cuts at: 2160 2160
Size: 5425
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SfcI C'TryA_G
Cuts at: 826 1045 2516 2773 3908 4589 4780 826
Size: 219 1471 257 1135 681 191 1471
Fragments arranged by size:
1471 1471 1135 681 257 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2057 2348 2752 1872
Size: 185 291 404 4545
Fragments arranged by size:
4545 404 291 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3529 4430 4692 820
Size: 197 926 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 2269 1517
Size: 752 4673
StyI C'CwwG_G
Cuts at: 513 1065 2233 2682 2864 513
Size: 552 1168 449 182 3074
Fragments arranged by size:
3074 1168 552 449 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2304 2315 3452 3469 3622 3807 4252
Size: 1255 724 11 1137 17 153 185 445
Cuts at: 4252 325
Size: 1498
Fragments arranged by size:
1498 1255 1137 724 445 185 153 17
11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2482 2598 3671
Size: 274 80 33 51 1443 505 116 1073
Cuts at: 3671 5383 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1073 505 274 138 116 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2580 2612 5385 5417 2580
Size: 32 2773 32 2588
Fragments arranged by size:
2773 2588 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5425
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5425
XmaI C'CCGG_G
Cuts at: 1870 2055 2346 2750 1870
Size: 185 291 404 4545
Fragments arranged by size:
4545 404 291 185
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI