Restriction map for
pFN21AE0553
(Circular) (Six-base) MAPSORT of: pFN21AE0553.seq Check: 9761 from: 1 to: 5776
pFN21AE0553 5776 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3075 3903 4276 1563
Size: 1512 828 373 3063
Fragments arranged by size:
3063 1512 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2804 3839 5223 5491 1632
Size: 1172 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1172 1035 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2833 4675 5652 1203
Size: 1630 1842 977 1327
Fragments arranged by size:
1842 1630 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 2834 2972 1166
Size: 1668 138 3970
Fragments arranged by size:
3970 1668 138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2227 2258 1184
Size: 1043 31 4702
Fragments arranged by size:
4702 1043 31
AlwNI CAG_nnn'CTG
Cuts at: 3519 5091 3519
Size: 1572 4204
ApaI G_GGCC'C
Cuts at: 2923 2923
Size: 5776
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 2321 2459 2522 3085 3311 3463 5572 1493
Size: 828 138 63 563 226 152 2109 1697
Fragments arranged by size:
2109 1697 828 563 226 152 138 63
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 3101 1870
Size: 73 1158 4545
Fragments arranged by size:
4545 1158 73
BamHI G'GATC_C
Cuts at: 2584 3106 3473 2584
Size: 522 367 4887
Fragments arranged by size:
4887 522 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2637 2681 3097 4552 618
Size: 325 217 627 850 44 416 1455 1842
Fragments arranged by size:
1842 1455 850 627 416 325 217 44
BanII G_rGCy'C
Cuts at: 1391 2434 2777 2923 3095 1391
Size: 1043 343 146 172 4072
Fragments arranged by size:
4072 1043 343 172 146
BbeI G_GCGC'C
Cuts at: 2641 2641
Size: 5776
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2290 3668 4878 2290
Size: 1378 1210 3188
Fragments arranged by size:
3188 1378 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BfrBI ATG'CAT
Cuts at: 2540 2540
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4387 136
Size: 107 122 71 631 605 2715 1525
Fragments arranged by size:
2715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2742 5771 2742
Size: 3029 2747
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2923 3837 4993 1790
Size: 1133 914 1156 2573
Fragments arranged by size:
2573 1156 1133 914
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 3507 4465
Size: 416 53 83 399 24 975 1115 958
Cuts at: 4465 442
Size: 1753
Fragments arranged by size:
1753 1115 975 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2791 3044 4436 1239
Size: 1552 253 1392 2579
Fragments arranged by size:
2579 1552 1392 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 4766 5064 3901
Size: 865 298 4613
Fragments arranged by size:
4613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2836 3567 493
Size: 711 1632 731 2702
Fragments arranged by size:
2702 1632 731 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2638 3583 3965 4600
Size: 53 83 186 936 1105 945 382 635
Cuts at: 4600 275
Size: 1451
Fragments arranged by size:
1451 1105 945 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 2421 2451 1178
Size: 30 165 30 621 30 367 30 4503
Fragments arranged by size:
4503 621 367 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3032 1186
Size: 1846 3930
BseYI C'CCAG_C
Cuts at: 2472 2915 2961 4979 5475 2472
Size: 443 46 2018 496 2773
Fragments arranged by size:
2773 2018 496 443 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2531 1163
Size: 573 795 4408
Fragments arranged by size:
4408 795 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 3024 3837 3922 4993 2337
Size: 687 813 85 1071 3120
Fragments arranged by size:
3120 1071 813 687 85
BsmI GAATG_Cn'
Cuts at: 2540 3287 3380 2540
Size: 747 93 4936
Fragments arranged by size:
4936 747 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2434 2777 2923 3024 3095 3837
Size: 399 547 97 343 146 101 71 742
Cuts at: 3837 3922 4993 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 399 343 146 101
97 85 71
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 2934 3665 5395 2934
Size: 731 1730 3315
Fragments arranged by size:
3315 1730 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3131 844
Size: 2287 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 2139 3663 1394
Size: 429 212 104 1524 3507
Fragments arranged by size:
3507 1524 429 212 104
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 2487 96
Size: 2391 3385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2509 2905 794
Size: 1715 396 3665
Fragments arranged by size:
3665 1715 396
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2584 2742 3106 3473 3858 3875 5327 5413
Size: 612 158 364 367 385 17 1452 86
Cuts at: 5413 5425 5771 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 612 385 367 364 346 158
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2162 2624 4783 809
Size: 507 846 462 2159 1802
Fragments arranged by size:
2159 1802 846 507 462
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 3223 4112
Size: 54 1425 50 275 18 486 907 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 907 889 486 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3706 1353
Size: 2353 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2350 4363 4737 4883 387
Size: 1064 138 761 2013 374 146 1280
Fragments arranged by size:
2013 1280 1064 761 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2791 2804 3044 3839 4436 5223 5491
Size: 393 1159 13 240 795 597 787 268
Cuts at: 5491 1239
Size: 1524
Fragments arranged by size:
1524 1159 795 787 597 393 268 240
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2885 2885
Size: 5776
EcoO109I rG'GnC_Cy
Cuts at: 2689 2919 2689
Size: 230 5546
EcoRI G'AATT_C
Cuts at: 2321 3085 3463 2321
Size: 764 378 4634
Fragments arranged by size:
4634 764 378
FspI TGC'GCA
Cuts at: 2846 4282 2846
Size: 1436 4340
HaeII r_GCGC'y
Cuts at: 1678 2408 2641 2671 2897 4923 1678
Size: 730 233 30 226 2026 2531
Fragments arranged by size:
2531 2026 730 233 226 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2832 2864 4417
Size: 32 135 32 436 32 792 32 1553
Cuts at: 4417 4449 4491 4523 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1553 792 436 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KasI G'GCGC_C
Cuts at: 2637 2637
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2072 2709 4890 5074 1305
Size: 767 637 2181 184 2007
Fragments arranged by size:
2181 2007 767 637 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2340 2834 2972 3734 4093 4252 518
Size: 648 1174 494 138 762 359 159 2042
Fragments arranged by size:
2042 1174 762 648 494 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2242 3192 3869 5017 5262 1606
Size: 636 950 677 1148 245 2120
Fragments arranged by size:
2120 1148 950 677 636 245
NarI GG'CG_CC
Cuts at: 2638 2638
Size: 5776
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NsiI A_TGCA'T
Cuts at: 2542 2542
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3003 1348
Size: 1655 4121
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2707 1108
Size: 1599 4177
PfoI T'CCnGG_A
Cuts at: 2282 2768 2282
Size: 486 5290
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PmlI CAC'GTG
Cuts at: 2836 2836
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2067 2099 3845 3877 5358 5390 2067
Size: 32 1746 32 1481 32 2453
Fragments arranged by size:
2453 1746 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2689 2689
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 2919 2919
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2414 3128 830
Size: 1584 714 3478
Fragments arranged by size:
3478 1584 714
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2242 2242
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 2957 4024 5736 2957
Size: 1067 1712 2997
Fragments arranged by size:
2997 1712 1067
SexAI A'CCwGG_T
Cuts at: 2270 2270
Size: 5776
SfcI C'TryA_G
Cuts at: 826 1045 2410 2746 3124 4259 4940 5131 826
Size: 219 1365 336 378 1135 681 191 1471
Fragments arranged by size:
1471 1365 1135 681 378 336 219 191
SfoI GGC'GCC
Cuts at: 2639 2639
Size: 5776
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3880 4781 5043 820
Size: 197 926 1937 901 262 1553
Fragments arranged by size:
1937 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2513 3215 513
Size: 552 1448 702 3074
Fragments arranged by size:
3074 1448 702 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3803 3820 3973 4158 4603 325
Size: 1255 2223 17 153 185 445 1498
Fragments arranged by size:
2223 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 2955 4022
Size: 274 80 33 51 1443 510 468 1067
Cuts at: 4022 5734 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1067 510 468 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 5736 5768 2421
Size: 32 3283 32 2429
Fragments arranged by size:
3283 2429 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3005 3035 3005
Size: 30 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI