Restriction map for
pFN21AE0552
(Circular) (Six-base) MAPSORT of: pFN21AE0552.seq Check: 8338 from: 1 to: 5611
pFN21AE0552 5611 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2649 2954 1937
Size: 712 305 4594
Fragments arranged by size:
4594 712 305
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 3738 4111 1563
Size: 2175 373 3063
Fragments arranged by size:
3063 2175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 2498 3674 5058 5326 1632
Size: 848 18 1176 1384 268 1917
Fragments arranged by size:
1917 1384 1176 848 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5414
AflIII A'CryG_T
Cuts at: 1203 2770 4510 5487 1203
Size: 1567 1740 977 1327
Fragments arranged by size:
1740 1567 1327 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 2828 1166
Size: 1662 3949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 2491 3354 4926 2491
Size: 863 1572 3176
Fragments arranged by size:
3176 1572 863
ApaLI G'TGCA_C
Cuts at: 3668 4824 3668
Size: 1156 4455
ApoI r'AATT_y
Cuts at: 1493 2920 3146 3298 5407 1493
Size: 1427 226 152 2109 1697
Fragments arranged by size:
2109 1697 1427 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2230 2305 2936 1870
Size: 73 287 75 631 4545
Fragments arranged by size:
4545 631 287 75 73
BamHI G'GATC_C
Cuts at: 2941 3308 2941
Size: 367 5244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2043 2167 2932 4387 618
Size: 325 217 627 256 124 765 1455 1842
Fragments arranged by size:
1842 1455 765 627 325 256 217 124
BanII G_rGCy'C
Cuts at: 1391 2304 2930 1391
Size: 913 626 4072
Fragments arranged by size:
4072 913 626
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3802 3836 1903
Size: 34 1865 34 3678
Fragments arranged by size:
3678 1865 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3503 4713 3503
Size: 1210 4401
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4222 136
Size: 107 122 71 631 605 2550 1525
Fragments arranged by size:
2550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5606 5606
Size: 5611
BlpI GC'TnA_GC
Cuts at: 3028 3028
Size: 5611
Bme1580I G_kGCm'C
Cuts at: 1790 3672 4828 1790
Size: 1882 1156 2573
Fragments arranged by size:
2573 1882 1156
BmgBI CAC'GTC
Cuts at: 1318 2266 1318
Size: 948 4663
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2011 2687 2701
Size: 416 53 83 399 24 594 676 14
Cuts at: 2701 3342 4300 442
Size: 641 958 1753
Fragments arranged by size:
1753 958 676 641 594 416 399 83
53 24 14
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2175 2639 4271 1239
Size: 936 464 1632 2579
Fragments arranged by size:
2579 1632 936 464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3736 4601 4899 3736
Size: 865 298 4448
Fragments arranged by size:
4448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4274 882
Size: 617 2775 2219
Fragments arranged by size:
2775 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2026 3402 493
Size: 711 822 1376 2702
Fragments arranged by size:
2702 1376 822 711
BsaBI GATnn'nnATC
Cuts at: 2366 5605 2366
Size: 3239 2372
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3418 3800 4435 275
Size: 53 83 186 936 1885 382 635 1451
Fragments arranged by size:
1885 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2544 4044 4442 4716 4863 5274 2544
Size: 1500 398 274 147 411 2881
Fragments arranged by size:
2881 1500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5386
Fragments arranged by size:
5386 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5611
BseYI C'CCAG_C
Cuts at: 2279 2296 2335 2583 4814 5310 2279
Size: 17 39 248 2231 496 2580
Fragments arranged by size:
2580 2231 496 248 39 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 2504 2765 1163
Size: 573 435 333 261 4009
Fragments arranged by size:
4009 573 435 333 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3061 3822 4850 1540
Size: 275 186 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3672 3757 4828 3672
Size: 85 1071 4455
Fragments arranged by size:
4455 1071 85
BsiWI C'GTAC_G
Cuts at: 2256 2256
Size: 5611
BsmI GAATG_Cn'
Cuts at: 2009 2839 3122 3215 2009
Size: 830 283 93 4405
Fragments arranged by size:
4405 830 283 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2221 2221
Size: 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2930 3672 3757 4828 1391
Size: 399 514 626 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 626 514 399 85
BspEI T'CCGG_A
Cuts at: 2544 5274 2544
Size: 2730 2881
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2966 844
Size: 2122 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2036 3498 1394
Size: 429 213 1462 3507
Fragments arranged by size:
3507 1462 429 213
BsrDI GCAATG_nn'
Cuts at: 65 1248 4106 4280 65
Size: 1183 2858 174 1396
Fragments arranged by size:
2858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 4255 4442 1700
Size: 110 245 2200 187 2869
Fragments arranged by size:
2869 2200 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5611
BssSI C'ACGA_G
Cuts at: 2381 3671 4683 2381
Size: 1290 1012 3309
Fragments arranged by size:
3309 1290 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2491 794
Size: 1697 3914
BstBI TT'CG_AA
Cuts at: 4415 4415
Size: 5611
BstEII G'GTnAC_C
Cuts at: 1184 2527 1184
Size: 1343 4268
BstYI r'GATC_y
Cuts at: 1972 2547 2941 3308 3693 3710 5162 5248 5260
Size: 575 394 367 385 17 1452 86 12
Cuts at: 5260 5606 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 575 394 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2561 2665 2762 513
Size: 552 939 557 104 97 3362
Fragments arranged by size:
3362 939 557 552 104 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4872
Fragments arranged by size:
4872 683 56
BtsI GCAGTG_nn'
Cuts at: 3127 3921 3941 3127
Size: 794 20 4797
Fragments arranged by size:
4797 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2525 4618 809
Size: 507 1209 2093 1802
Fragments arranged by size:
2093 1802 1209 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2409 2779
Size: 54 1425 50 275 18 201 378 370
Cuts at: 2779 3058 3947 5576 8
Size: 279 889 1629 43
Fragments arranged by size:
1629 1425 889 378 370 279 275 201
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3058 1537
Size: 275 1246 4090
Fragments arranged by size:
4090 1246 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3541 1353
Size: 2188 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4198 4572 4718 387
Size: 1064 138 2609 374 146 1280
Fragments arranged by size:
2609 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2480 2498 2639 3674 4271 5058
Size: 393 543 305 18 141 1035 597 787
Cuts at: 5058 5326 1239
Size: 268 1524
Fragments arranged by size:
1524 1035 787 597 543 393 305 268
141 18
EcoO109I rG'GnC_Cy
Cuts at: 2695 2695
Size: 5611
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 5611
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 4758 1678
Size: 3080 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 2213 2245 4252
Size: 32 135 32 448 32 161 32 2007
Cuts at: 4252 4284 4326 4358 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2007 448 161 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2955 3201 669
Size: 988 281 1017 246 3079
Fragments arranged by size:
3079 1017 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5611
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2112 4725 4909 1305
Size: 807 2613 184 2007
Fragments arranged by size:
2613 2007 807 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2781 10
Size: 54 1425 343 949 2840
Fragments arranged by size:
2840 1425 949 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 2249 2828 3569 3928 4087 518
Size: 648 903 180 579 741 359 159 2042
Fragments arranged by size:
2042 903 741 648 579 359 180 159
MspA1I CmG'CkG
Cuts at: 1606 2713 2764 3027 3704 4852 5097 1606
Size: 1107 51 263 677 1148 245 2120
Fragments arranged by size:
2120 1148 1107 677 263 245 51
NaeI GCC'GGC
Cuts at: 2057 2057
Size: 5611
NcoI C'CATG_G
Cuts at: 513 1065 2004 2561 2665 513
Size: 552 939 557 104 3459
Fragments arranged by size:
3459 939 557 552 104
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NgoMIV G'CCGG_C
Cuts at: 2055 2055
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2150 2774 2969 4514 1132
Size: 97 921 624 195 1545 2229
Fragments arranged by size:
2229 1545 921 624 195 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 2770 4510 2770
Size: 1740 3871
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2029 1108
Size: 921 4690
PfoI T'CCnGG_A
Cuts at: 2685 2685
Size: 5611
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PmlI CAC'GTG
Cuts at: 2026 2026
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 5193 5225 3680
Size: 32 1481 32 4066
Fragments arranged by size:
4066 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2695 2695
Size: 5611
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PspXI vC'TCGA_Gb
Cuts at: 2230 2230
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2963 830
Size: 2133 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
SacII CC_GC'GG
Cuts at: 2765 2765
Size: 5611
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SanDI GG'GwC_CC
Cuts at: 2695 2695
Size: 5611
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SfcI C'TryA_G
Cuts at: 826 1045 2959 4094 4775 4966 826
Size: 219 1914 1135 681 191 1471
Fragments arranged by size:
1914 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5611
SmaI CCC'GGG
Cuts at: 1872 2938 1872
Size: 1066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2230 3715 4616 4878 820
Size: 197 926 287 1485 901 262 1553
Fragments arranged by size:
1553 1485 926 901 287 262 197
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5611
SphI G_CATG'C
Cuts at: 1132 2969 1132
Size: 1837 3774
SspI AAT'ATT
Cuts at: 5 52 3535 5
Size: 47 3483 2081
Fragments arranged by size:
3483 2081 47
StuI AGG'CCT
Cuts at: 1517 2102 1517
Size: 585 5026
StyI C'CwwG_G
Cuts at: 513 1065 2004 2561 2665 3050 513
Size: 552 939 557 104 385 3074
Fragments arranged by size:
3074 939 557 552 385 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 3638 3655 3808 3993 4438 325
Size: 1255 531 1527 17 153 185 445 1498
Fragments arranged by size:
1527 1498 1255 531 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 2355 3857
Size: 274 80 33 51 1443 102 276 1502
Cuts at: 3857 5569 96
Size: 1712 138
Fragments arranged by size:
1712 1502 1443 276 274 138 102 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2667 2699 5571 5603 2667
Size: 32 2872 32 2675
Fragments arranged by size:
2872 2675 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2693 1316
Size: 1377 4234
XbaI T'CTAG_A
Cuts at: 2947 2947
Size: 5611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2568 2795 2568
Size: 227 5384
XhoI C'TCGA_G
Cuts at: 1943 2230 1943
Size: 287 5324
XmaI C'CCGG_G
Cuts at: 1870 2936 1870
Size: 1066 4545
XmnI GAAnn'nnTTC
Cuts at: 2321 3740 2321
Size: 1419 4192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI SacII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PspOMI PsrI PvuII RsrII SacI SapI SexAI
SfiI SfoI SrfI SwaI