Restriction map for
pFN21AE0547
(Circular) (Six-base) MAPSORT of: pFN21AE0547.seq Check: 6816 from: 1 to: 5974
pFN21AE0547 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2903 2984 4037 5421 5689 1632
Size: 1271 81 1053 1384 268 1917
Fragments arranged by size:
1917 1384 1271 1053 268 81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 2640 2709 4873 5850 1203
Size: 1437 69 2164 977 1327
Fragments arranged by size:
2164 1437 1327 977 69
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 5942
AlwNI CAG_nnn'CTG
Cuts at: 2406 2881 3214 3717 5289 2406
Size: 475 333 503 1572 3091
Fragments arranged by size:
3091 1572 503 475 333
ApaI G_GGCC'C
Cuts at: 3222 3222
Size: 5974
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2107 3283 3509 3661 5770 1493
Size: 614 1176 226 152 2109 1697
Fragments arranged by size:
2109 1697 1176 614 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3048 4528 160
Size: 872 2016 1480 1606
Fragments arranged by size:
2016 1606 1480 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2487 2686 3244 3299 1870
Size: 73 544 199 558 55 4545
Fragments arranged by size:
4545 558 544 199 73 55
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2893 3295 4750 618
Size: 325 217 627 1106 402 1455 1842
Fragments arranged by size:
1842 1455 1106 627 402 325 217
BanII G_rGCy'C
Cuts at: 1391 2396 3222 3293 1391
Size: 1005 826 71 4072
Fragments arranged by size:
4072 1005 826 71
BbeI G_GCGC'C
Cuts at: 2897 2897
Size: 5974
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2830 2864 2969 3003 3216 3250 4165
Size: 34 893 34 105 34 213 34 915
Cuts at: 4165 4199 1903
Size: 34 3678
Fragments arranged by size:
3678 915 893 213 105 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 2052 1548
Size: 504 5470
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 3222 4035 5191 1790
Size: 1432 813 1156 2573
Fragments arranged by size:
2573 1432 1156 813
BmgBI CAC'GTC
Cuts at: 1318 3006 1318
Size: 1688 4286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3275
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2831 4634 1239
Size: 1049 543 1803 2579
Fragments arranged by size:
2579 1803 1049 543
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3017 4099 4964 5262 3017
Size: 1082 865 298 3729
Fragments arranged by size:
3729 1082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2539 2619 3765 493
Size: 711 1335 80 1146 2702
Fragments arranged by size:
2702 1335 1146 711 80
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2894 3781 4163 4798
Size: 53 83 186 936 1361 887 382 635
Cuts at: 4798 275
Size: 1451
Fragments arranged by size:
1451 1361 936 887 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2385 4407 4805 5079 5226 5637 2385
Size: 2022 398 274 147 411 2722
Fragments arranged by size:
2722 2022 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 1186
Size: 1207 4767
BseYI C'CCAG_C
Cuts at: 5177 5673 5177
Size: 496 5478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2653 2894 1163
Size: 573 917 241 4243
Fragments arranged by size:
4243 917 573 241
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3236 3424 4185 5213 1540
Size: 275 186 1235 188 761 1028 2301
Fragments arranged by size:
2301 1235 1028 761 275 188 186
BsiHKAI G_wGCw'C
Cuts at: 2819 4035 4120 5191 2819
Size: 1216 85 1071 3602
Fragments arranged by size:
3602 1216 1071 85
BsmI GAATG_Cn'
Cuts at: 2933 2992 3485 3578 2933
Size: 59 493 93 5329
Fragments arranged by size:
5329 493 93 59
BsmBI CGTCTCn'nnnn_
Cuts at: 3003 3003
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 2819 3222 3293 4035 4120 5191
Size: 399 606 423 403 71 742 85 1071
Cuts at: 5191 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 606 423 403 399 85
71
BspEI T'CCGG_A
Cuts at: 2385 5637 2385
Size: 3252 2722
BspHI T'CATG_A
Cuts at: 2412 3863 5593 2412
Size: 1451 1730 2793
Fragments arranged by size:
2793 1730 1451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2601 2805 3329 844
Size: 1757 204 524 3489
Fragments arranged by size:
3489 1757 524 204
BsrBI CCG'CTC
Cuts at: 1394 1823 3254 3861 1394
Size: 429 1431 607 3507
Fragments arranged by size:
3507 1431 607 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2661 2896 4618 4805 1700
Size: 110 851 235 1722 187 2869
Fragments arranged by size:
2869 1722 851 235 187 110
BsrGI T'GTAC_A
Cuts at: 96 2643 96
Size: 2547 3427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 2833 1184
Size: 1649 4325
BstXI CCAn_nnnn'nTGG
Cuts at: 2701 2771 2701
Size: 70 5904
BstYI r'GATC_y
Cuts at: 1972 2856 3304 3671 4056 4073 5525 5611 5623
Size: 884 448 367 385 17 1452 86 12
Cuts at: 5623 5969 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 884 448 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3117 3117
Size: 5974
BtgI C'CryG_G
Cuts at: 513 1065 2004 2770 2821 513
Size: 552 939 766 51 3666
Fragments arranged by size:
3666 939 766 552 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3049 487
Size: 683 56 1823 3412
Fragments arranged by size:
3412 1823 683 56
BtsI GCAGTG_nn'
Cuts at: 2627 2917 3012 3490 4284 4304 2627
Size: 290 95 478 794 20 4297
Fragments arranged by size:
4297 794 478 290 95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2186 4981 809
Size: 507 870 2795 1802
Fragments arranged by size:
2795 1802 870 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939
Size: 54 1425 50 275 18 1591 889 1629
Cuts at: 5939 8
Size: 43
Fragments arranged by size:
1629 1591 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 3904 1353
Size: 1031 1520 3423
Fragments arranged by size:
3423 1520 1031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2831 2903 2984 4037 4634 5421
Size: 393 656 543 72 81 1053 597 787
Cuts at: 5421 5689 1239
Size: 268 1524
Fragments arranged by size:
1524 1053 787 656 597 543 393 268
81 72
EcoO109I rG'GnC_Cy
Cuts at: 2227 3218 2227
Size: 991 4983
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
EcoRV GAT'ATC
Cuts at: 2035 2165 2035
Size: 130 5844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2938 2464
Size: 474 5500
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 2897 5121 1678
Size: 1219 2224 2531
Fragments arranged by size:
2531 2224 1219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2064 2096 2154 2186 3184
Size: 32 135 32 492 32 58 32 998
Cuts at: 3184 3216 4615 4647 4689 4721 1373
Size: 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1399 998 492 135 58 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2191 3318 3564 669
Size: 988 281 253 1127 246 3079
Fragments arranged by size:
3079 1127 988 281 253 246
HindIII A'AGCT_T
Cuts at: 748 2648 748
Size: 1900 4074
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KasI G'GCGC_C
Cuts at: 2893 2893
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2345 2769 3932 4291 4450 518
Size: 648 1179 424 1163 359 159 2042
Fragments arranged by size:
2042 1179 1163 648 424 359 159
MspA1I CmG'CkG
Cuts at: 1606 2357 2823 3390 4067 5215 5460 1606
Size: 751 466 567 677 1148 245 2120
Fragments arranged by size:
2120 1148 751 677 567 466 245
NarI GG'CG_CC
Cuts at: 2894 2894
Size: 5974
NcoI C'CATG_G
Cuts at: 513 1065 2004 2770 513
Size: 552 939 766 3717
Fragments arranged by size:
3717 939 766 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2644 2713 3332 4877 1132
Size: 97 1415 69 619 1545 2229
Fragments arranged by size:
2229 1545 1415 619 97 69
PasI CC'CwG_GG
Cuts at: 1348 2434 3166 1348
Size: 1086 732 4156
Fragments arranged by size:
4156 1086 732
PciI A'CATG_T
Cuts at: 2640 2709 4873 2640
Size: 69 2164 3741
Fragments arranged by size:
3741 2164 69
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2406 1108
Size: 1298 4676
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2227 2227
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 3218 3218
Size: 5974
PstI C_TGCA'G
Cuts at: 830 3326 830
Size: 2496 3478
PvuI CG_AT'CG
Cuts at: 2001 3236 2001
Size: 1235 4739
SacII CC_GC'GG
Cuts at: 2824 2824
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SapI GCTCTTCn'nnn_
Cuts at: 2384 2384
Size: 5974
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 3322 4457 5138 5329 826
Size: 219 2277 1135 681 191 1471
Fragments arranged by size:
2277 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2895 2895
Size: 5974
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 2896 1700
Size: 1196 4778
SmaI CCC'GGG
Cuts at: 1872 2489 3301 1872
Size: 617 812 4545
Fragments arranged by size:
4545 812 617
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3032 4078 4979 5241 820
Size: 197 926 1089 1046 901 262 1553
Fragments arranged by size:
1553 1089 1046 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 2770 3413 513
Size: 552 939 766 643 3074
Fragments arranged by size:
3074 939 766 643 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3039 4001 4018 4171 4356 4801 325
Size: 1255 1459 962 17 153 185 445 1498
Fragments arranged by size:
1498 1459 1255 962 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2643 3036 4220
Size: 274 80 33 51 1443 666 393 1184
Cuts at: 4220 5932 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1184 666 393 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2608 2640 5934 5966 2608
Size: 32 3294 32 2616
Fragments arranged by size:
3294 2616 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3096 3096
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 2487 3299 1870
Size: 617 812 4545
Fragments arranged by size:
4545 812 617
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI