Restriction map for
pFN21AE0544
(Circular) (Six-base) MAPSORT of: pFN21AE0544.seq Check: 8138 from: 1 to: 5986
pFN21AE0544 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2998 2998
Size: 5986
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2733 3329 1937
Size: 796 596 4594
Fragments arranged by size:
4594 796 596
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2254 2642 3101 4049 5433 5701 1632
Size: 622 388 459 948 1384 268 1917
Fragments arranged by size:
1917 1384 948 622 459 388 268
AfeI AGC'GCT
Cuts at: 2627 2627
Size: 5986
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 4885 5862 1203
Size: 3682 977 1327
Fragments arranged by size:
3682 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3246 1184
Size: 2062 3924
AlwNI CAG_nnn'CTG
Cuts at: 2881 2963 3729 5301 2881
Size: 82 766 1572 3566
Fragments arranged by size:
3566 1572 766 82
ApaI G_GGCC'C
Cuts at: 2053 2053
Size: 5986
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 3295 3521 3673 5782 1493
Size: 1802 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2711 3311 1870
Size: 73 768 600 4545
Fragments arranged by size:
4545 768 600 73
AvrII C'CTAG_G
Cuts at: 2257 2257
Size: 5986
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2010 2935 3307 4762 618
Size: 325 217 627 223 925 372 1455 1842
Fragments arranged by size:
1842 1455 925 627 372 325 223 217
BanII G_rGCy'C
Cuts at: 1391 2053 3305 1391
Size: 662 1252 4072
Fragments arranged by size:
4072 1252 662
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2217 928
Size: 971 318 4697
Fragments arranged by size:
4697 971 318
BbvCI CC'TCA_GC
Cuts at: 3269 3269
Size: 5986
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 2437 1548
Size: 889 5097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2691 5981 2691
Size: 3290 2696
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 2053 4047 5203 1790
Size: 263 1994 1156 2573
Fragments arranged by size:
2573 1994 1156 263
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2801 3717 4675
Size: 416 53 83 399 24 1384 916 958
Cuts at: 4675 442
Size: 1753
Fragments arranged by size:
1753 1384 958 916 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2170 2429 4646 1239
Size: 926 5 259 2217 2579
Fragments arranged by size:
2579 2217 926 259 5
Bpu10I CC'TnA_GC
Cuts at: 3269 3269
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2792 4111 4976 5274 2216
Size: 576 1319 865 298 2928
Fragments arranged by size:
2928 1319 865 576 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2392 2960 3195 4649 882
Size: 617 893 568 235 1454 2219
Fragments arranged by size:
2219 1454 893 617 568 235
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3134 3164 1178
Size: 30 165 30 1731 30 4000
Fragments arranged by size:
4000 1731 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 1186
Size: 1516 4470
BseYI C'CCAG_C
Cuts at: 2159 2198 2874 3282 5189 5685 2159
Size: 39 676 408 1907 496 2460
Fragments arranged by size:
2460 1907 676 496 408 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 1163
Size: 573 471 4942
Fragments arranged by size:
4942 573 471
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3171 3436 4197 5225 1540
Size: 275 186 1170 265 761 1028 2301
Fragments arranged by size:
2301 1170 1028 761 275 265 186
BsiHKAI G_wGCw'C
Cuts at: 2151 4047 4132 5203 2151
Size: 1896 85 1071 2934
Fragments arranged by size:
2934 1896 1071 85
BsmI GAATG_Cn'
Cuts at: 2484 2609 2863 3497 3590 2484
Size: 125 254 634 93 4880
Fragments arranged by size:
4880 634 254 125 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2151 3305 4047 4132 5203 1391
Size: 399 263 98 1154 742 85 1071 2174
Fragments arranged by size:
2174 1154 1071 742 399 263 98 85
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 2763 2865 3875 5605 2763
Size: 102 1010 1730 3144
Fragments arranged by size:
3144 1730 1010 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2337 2998 3341 844
Size: 1385 108 661 343 3489
Fragments arranged by size:
3489 1385 661 343 108
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4481 4655 65
Size: 1183 3233 174 1396
Fragments arranged by size:
3233 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2133 4630 4817 1700
Size: 110 323 2497 187 2869
Fragments arranged by size:
2869 2497 323 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 2940 4046 5058 2940
Size: 1106 1012 3868
Fragments arranged by size:
3868 1106 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 2240 1184
Size: 1056 4930
BstYI r'GATC_y
Cuts at: 1972 2691 3316 3683 4068 4085 5537 5623 5635
Size: 719 625 367 385 17 1452 86 12
Cuts at: 5635 5981 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 719 625 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2307 2595 2664 3066 513
Size: 552 1242 288 69 402 3433
Fragments arranged by size:
3433 1242 552 402 288 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2255 3502 4296 4316 2255
Size: 1247 794 20 3925
Fragments arranged by size:
3925 1247 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2384 4993 809
Size: 507 1068 2609 1802
Fragments arranged by size:
2609 1802 1068 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2667 2829 3054
Size: 54 1425 50 275 18 837 162 225
Cuts at: 3054 3168 3433 4322 5951 8
Size: 114 265 889 1629 43
Fragments arranged by size:
1629 1425 889 837 275 265 225 162
114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3168 3433 1537
Size: 275 1356 265 4090
Fragments arranged by size:
4090 1356 275 265
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 2414 3916 1353
Size: 680 381 1502 3423
Fragments arranged by size:
3423 1502 680 381
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2170 2254 2429 2642 3101 4049
Size: 393 533 5 84 175 213 459 948
Cuts at: 4049 4646 5433 5701 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 948 787 597 533 459 393 268
213 175 84 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3083 3083
Size: 5986
EcoO109I rG'GnC_Cy
Cuts at: 2019 2041 2049 2050 3137 2019
Size: 22 8 1 1087 4868
Fragments arranged by size:
4868 1087 22 8 1
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 2629 5133 1678
Size: 951 2504 2531
Fragments arranged by size:
2531 2504 951
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2643 2675 2676 2708 2959
Size: 32 135 32 1071 32 1 32 251
Cuts at: 2959 2991 3027 3059 4627 4659 4701 4733 1373
Size: 32 36 32 1568 32 42 32 2626
Fragments arranged by size:
2626 1568 1071 251 135 42 36 32
32 32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2234 748
Size: 1486 4500
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2627 2931 5100 5284 1305
Size: 1322 304 2169 184 2007
Fragments arranged by size:
2169 2007 1322 304 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 2831 10
Size: 54 1425 343 837 162 3165
Fragments arranged by size:
3165 1425 837 343 162 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2168 3944 4303 4462 518
Size: 648 894 108 1776 359 159 2042
Fragments arranged by size:
2042 1776 894 648 359 159 108
MspA1I CmG'CkG
Cuts at: 1606 2317 2878 2960 3402 4079 5227 5472 1606
Size: 711 561 82 442 677 1148 245 2120
Fragments arranged by size:
2120 1148 711 677 561 442 245 82
NcoI C'CATG_G
Cuts at: 513 1065 2307 2595 2664 513
Size: 552 1242 288 69 3835
Fragments arranged by size:
3835 1242 552 288 69
NdeI CA'TA_TG
Cuts at: 387 3112 387
Size: 2725 3261
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2805 3344 4889 1132
Size: 97 1576 539 1545 2229
Fragments arranged by size:
2229 1576 1545 539 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2581 1108
Size: 1473 4513
PfoI T'CCnGG_A
Cuts at: 2406 2406
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2041
Size: 5986
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PspOMI G'GGCC_C
Cuts at: 2049 2049
Size: 5986
PstI C_TGCA'G
Cuts at: 830 2196 2841 3009 3338 830
Size: 1366 645 168 329 3478
Fragments arranged by size:
3478 1366 645 329 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2317 2878 2960 2317
Size: 561 82 5343
Fragments arranged by size:
5343 561 82
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 2192 2734 2837 3005 3238 3334 4469
Size: 219 1147 542 103 168 233 96 1135
Cuts at: 4469 5150 5341 826
Size: 681 191 1471
Fragments arranged by size:
1471 1147 1135 681 542 233 219 191
168 103 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2231 2807 4090 4991 5253 820
Size: 197 926 288 576 1283 901 262 1553
Fragments arranged by size:
1553 1283 926 901 576 288 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 3125 1517
Size: 1608 4378
StyI C'CwwG_G
Cuts at: 513 1065 2014 2123 2205 2257 2307 2595 2664
Size: 552 949 109 82 52 50 288 69
Cuts at: 2664 3246 3425 513
Size: 582 179 3074
Fragments arranged by size:
3074 949 582 552 288 179 109 82
69 52 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4013 4030 4183 4368 4813 325
Size: 1255 2433 17 153 185 445 1498
Fragments arranged by size:
2433 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4232 5944 96
Size: 274 80 33 51 1443 2255 1712 138
Fragments arranged by size:
2255 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2314 2602 3049 2167
Size: 147 288 447 5104
Fragments arranged by size:
5104 447 288 147
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI