Restriction map for
pFN21AE0543
(Circular) (Six-base) MAPSORT of: pFN21AE0543.seq Check: 675 from: 1 to: 5650
pFN21AE0543 5650 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 3713 5097 5365 1632
Size: 698 1383 1384 268 1917
Fragments arranged by size:
1917 1384 1383 698 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 2781 4549 5526 1203
Size: 1578 1768 977 1327
Fragments arranged by size:
1768 1578 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 2020 2539 3393 4965 2020
Size: 519 854 1572 2705
Fragments arranged by size:
2705 1572 854 519
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2959 3185 3337 5446 1493
Size: 1466 226 152 2109 1697
Fragments arranged by size:
2109 1697 1466 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 2975 1870
Size: 73 229 803 4545
Fragments arranged by size:
4545 803 229 73
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2490 2564 2971 4426 618
Size: 325 217 627 703 74 407 1455 1842
Fragments arranged by size:
1842 1455 703 627 407 325 217 74
BanII G_rGCy'C
Cuts at: 1391 2064 2432 2550 2649 2969 1391
Size: 673 368 118 99 320 4072
Fragments arranged by size:
4072 673 368 320 118 99
BbeI G_GCGC'C
Cuts at: 2494 2494
Size: 5650
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2171 928
Size: 971 272 4407
Fragments arranged by size:
4407 971 272
BbvCI CC'TCA_GC
Cuts at: 2437 2437
Size: 5650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2657 4261 136
Size: 107 122 71 631 605 985 1604 1525
Fragments arranged by size:
1604 1525 985 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2869 3711 4867 1790
Size: 1079 842 1156 2573
Fragments arranged by size:
2573 1156 1079 842
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2217 2437 4310 1239
Size: 978 220 1873 2579
Fragments arranged by size:
2579 1873 978 220
Bpu10I CC'TnA_GC
Cuts at: 2437 2437
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2744 2883 3775 4640 4938 2366
Size: 378 139 892 865 298 3078
Fragments arranged by size:
3078 892 865 378 298 139
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2407 2491 2660 2723
Size: 53 83 186 936 874 84 169 63
Cuts at: 2723 3457 3839 4474 275
Size: 734 382 635 1451
Fragments arranged by size:
1451 936 874 734 635 382 186 169
84 83 63 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2564 1186
Size: 1378 4272
BseYI C'CCAG_C
Cuts at: 2339 2543 4853 5349 2339
Size: 204 2310 496 2640
Fragments arranged by size:
2640 2310 496 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5077
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2715 3100 3861 4889 1540
Size: 275 186 714 385 761 1028 2301
Fragments arranged by size:
2301 1028 761 714 385 275 186
BsiHKAI G_wGCw'C
Cuts at: 2649 3711 3796 4867 2649
Size: 1062 85 1071 3432
Fragments arranged by size:
3432 1071 1062 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2432 2550 2649 2869 2969 3711
Size: 399 274 368 118 99 220 100 742
Cuts at: 3711 3796 4867 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 274 220 118
100 99 85
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 2844 3539 5269 2844
Size: 695 1730 3225
Fragments arranged by size:
3225 1730 695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2084 2687 3005 844
Size: 1240 603 318 3489
Fragments arranged by size:
3489 1240 603 318
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2508 4145 4319 65
Size: 1183 1260 1637 174 1396
Fragments arranged by size:
1637 1396 1260 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2651 4294 4481 1700
Size: 110 841 1643 187 2869
Fragments arranged by size:
2869 1643 841 187 110
BsrGI T'GTAC_A
Cuts at: 96 2136 2784 96
Size: 2040 648 2962
Fragments arranged by size:
2962 2040 648
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 2063 3710 4722 2063
Size: 1647 1012 2991
Fragments arranged by size:
2991 1647 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 2071 2359 1184
Size: 887 288 4475
Fragments arranged by size:
4475 887 288
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2251 2095
Size: 156 5494
BstYI r'GATC_y
Cuts at: 1972 2017 2980 3347 3732 3749 5201 5287 5299
Size: 45 963 367 385 17 1452 86 12
Cuts at: 5299 5645 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 963 385 367 346 86 45
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2370 513
Size: 552 1305 3793
Fragments arranged by size:
3793 1305 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 2724 3166 3960 3980 2217
Size: 507 442 794 20 3887
Fragments arranged by size:
3887 794 507 442 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2491 2526 529
Size: 35 1927 35 3653
Fragments arranged by size:
3653 1927 35 35
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2256 2412
Size: 54 1425 50 275 18 267 159 156
Cuts at: 2412 3097 3986 5615 8
Size: 685 889 1629 43
Fragments arranged by size:
1629 1425 889 685 275 267 159 156
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3580 1353
Size: 2227 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2192 4237 4611 4757 387
Size: 1064 138 603 2045 374 146 1280
Fragments arranged by size:
2045 1280 1064 603 374 146 138
EcoICRI GAG'CTC
Cuts at: 2647 2647
Size: 5650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2330 2437 3713 4310 5097 5365
Size: 393 585 113 107 1276 597 787 268
Cuts at: 5365 1239
Size: 1524
Fragments arranged by size:
1524 1276 787 597 585 393 268 113
107
EcoNI CCTnn'n_nnAGG
Cuts at: 2774 2774
Size: 5650
EcoO109I rG'GnC_Cy
Cuts at: 2457 2536 2557 2457
Size: 79 21 5550
Fragments arranged by size:
5550 79 21
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 5650
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 2494 2856 4797 1678
Size: 816 362 1941 2531
Fragments arranged by size:
2531 1941 816 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2819 2851 2916 2948 4291
Size: 32 135 32 1247 32 65 32 1343
Cuts at: 4291 4323 4365 4397 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1343 1247 135 65 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KasI G'GCGC_C
Cuts at: 2490 2490
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4764 4948 1305
Size: 3459 184 2007
Fragments arranged by size:
3459 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 10
Size: 54 1425 343 267 3561
Fragments arranged by size:
3561 1425 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 3608 3967 4126 518
Size: 648 1209 1233 359 159 2042
Fragments arranged by size:
2042 1233 1209 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2302 2392 3066 3743 4891 5136 1606
Size: 696 90 674 677 1148 245 2120
Fragments arranged by size:
2120 1148 696 677 674 245 90
NarI GG'CG_CC
Cuts at: 2491 2491
Size: 5650
NcoI C'CATG_G
Cuts at: 513 1065 2370 513
Size: 552 1305 3793
Fragments arranged by size:
3793 1305 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2197 2785 3008 4553 1132
Size: 97 968 588 223 1545 2229
Fragments arranged by size:
2229 1545 968 588 223 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 2781 4549 2781
Size: 1768 3882
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2356 1108
Size: 1248 4402
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2457 2457
Size: 5650
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2115 2212 2397 3002 830
Size: 1285 97 185 605 3478
Fragments arranged by size:
3478 1285 605 185 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2392 2392
Size: 5650
SacI G_AGCT'C
Cuts at: 2649 2649
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2111 2208 2393 2551 2998 4133 4814
Size: 219 1066 97 185 158 447 1135 681
Cuts at: 4814 5005 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1066 681 447 219 191 185
158 97
SfoI GGC'GCC
Cuts at: 2492 2492
Size: 5650
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2381 2759 2898 3754 4655 4917
Size: 197 926 438 378 139 856 901 262
Cuts at: 4917 820
Size: 1553
Fragments arranged by size:
1553 926 901 856 438 378 262 197
139
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 2769 1517
Size: 1252 4398
StyI C'CwwG_G
Cuts at: 513 1065 2370 3089 513
Size: 552 1305 719 3074
Fragments arranged by size:
3074 1305 719 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2498 2552 3677 3694 3847 4032 4477
Size: 1255 918 54 1125 17 153 185 445
Cuts at: 4477 325
Size: 1498
Fragments arranged by size:
1498 1255 1125 918 445 185 153 54
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 2784 3896
Size: 274 80 33 51 1443 159 648 1112
Cuts at: 3896 5608 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1112 648 274 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2728 2986 2728
Size: 258 5392
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI