Restriction map for
pFN21AE0542
(Circular) (Six-base) MAPSORT of: pFN21AE0542.seq Check: 1437 from: 1 to: 5626
pFN21AE0542 5626 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 2411 2927 3689 5073 5341 1632
Size: 467 312 516 762 1384 268 1917
Fragments arranged by size:
1917 1384 762 516 467 312 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 2923 4525 5502 1203
Size: 1720 1602 977 1327
Fragments arranged by size:
1720 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 2657 1166
Size: 1491 4135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 2229 3369 4941 2229
Size: 1140 1572 2914
Fragments arranged by size:
2914 1572 1140
ApaLI G'TGCA_C
Cuts at: 2913 3683 4839 2913
Size: 770 1156 3700
Fragments arranged by size:
3700 1156 770
ApoI r'AATT_y
Cuts at: 1493 2408 2935 3161 3313 5422 1493
Size: 915 527 226 152 2109 1697
Fragments arranged by size:
2109 1697 915 527 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 1870
Size: 73 1008 4545
Fragments arranged by size:
4545 1008 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2431 2464 2431
Size: 33 5593
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 2947 4402 618
Size: 325 217 627 404 756 1455 1842
Fragments arranged by size:
1842 1455 756 627 404 325 217
BanII G_rGCy'C
Cuts at: 1391 2945 1391
Size: 1554 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2427 2524 2735 928
Size: 971 528 97 211 3819
Fragments arranged by size:
3819 971 528 211 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3817 3851 1903
Size: 34 1880 34 3678
Fragments arranged by size:
3678 1880 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2089 3518 4728 2089
Size: 1429 1210 2987
Fragments arranged by size:
2987 1429 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4237 136
Size: 107 122 71 631 605 2565 1525
Fragments arranged by size:
2565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2314 5621 2314
Size: 3307 2319
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 2917 3687 4843 1790
Size: 1127 770 1156 2573
Fragments arranged by size:
2573 1156 1127 770
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 4286 1239
Size: 944 2103 2579
Fragments arranged by size:
2579 2103 944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3751 4616 4914 3751
Size: 865 298 4463
Fragments arranged by size:
4463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3417 493
Size: 711 2213 2702
Fragments arranged by size:
2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2704 3433 3815 4450
Size: 53 83 186 936 1171 729 382 635
Cuts at: 4450 275
Size: 1451
Fragments arranged by size:
1451 1171 936 729 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5401
Fragments arranged by size:
5401 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5626
BseYI C'CCAG_C
Cuts at: 4829 5325 4829
Size: 496 5130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2652 1163
Size: 573 916 4137
Fragments arranged by size:
4137 916 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3837 4865 1540
Size: 275 186 1075 761 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2917 3687 3772 4843 2160
Size: 757 770 85 1071 2943
Fragments arranged by size:
2943 1071 770 757 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2917 2945 3687 3772 4843 1391
Size: 399 370 757 28 742 85 1071 2174
Fragments arranged by size:
2174 1071 757 742 399 370 85 28
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 3515 5245 3515
Size: 1730 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 844
Size: 2137 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2079 2519 4121 4295 65
Size: 1183 831 440 1602 174 1396
Fragments arranged by size:
1602 1396 1183 831 440 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4270 4457 1700
Size: 110 2460 187 2869
Fragments arranged by size:
2869 2460 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5626
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 2659 1184
Size: 1475 4151
BstYI r'GATC_y
Cuts at: 1972 2314 2956 3323 3708 3725 5177 5263 5275
Size: 342 642 367 385 17 1452 86 12
Cuts at: 5275 5621 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 642 385 367 346 342 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2055 2655 513
Size: 552 939 39 12 600 3484
Fragments arranged by size:
3484 939 600 552 39 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2493 487
Size: 683 56 1267 3620
Fragments arranged by size:
3620 1267 683 56
BtsI GCAGTG_nn'
Cuts at: 2841 3142 3936 3956 2841
Size: 301 794 20 4511
Fragments arranged by size:
4511 794 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2571 2586 2930 2571
Size: 15 344 5267
Fragments arranged by size:
5267 344 15
DraIII CAC_nnn'GTG
Cuts at: 2044 2155 2044
Size: 111 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2683 4633 809
Size: 507 1367 1950 1802
Fragments arranged by size:
1950 1802 1367 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3073 3962
Size: 54 1425 50 275 18 216 1027 889
Cuts at: 3962 5591 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1027 889 275 216 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3556 1353
Size: 2203 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2183 2411 2927 3689 4286 5073
Size: 393 467 84 228 516 762 597 787
Cuts at: 5073 5341 1239
Size: 268 1524
Fragments arranged by size:
1524 787 762 597 516 467 393 268
228 84
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2241 4267 4299 4341
Size: 32 135 32 637 32 2026 32 42
Cuts at: 4341 4373 1373
Size: 32 2626
Fragments arranged by size:
2626 2026 637 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2294 2970 3216 669
Size: 988 281 356 676 246 3079
Fragments arranged by size:
3079 988 676 356 281 246
HindIII A'AGCT_T
Cuts at: 748 2393 2600 748
Size: 1645 207 3774
Fragments arranged by size:
3774 1645 207
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4740 4924 1305
Size: 3435 184 2007
Fragments arranged by size:
3435 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3804
Fragments arranged by size:
3804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2657 2897 3584 3943 4102 518
Size: 648 1491 240 687 359 159 2042
Fragments arranged by size:
2042 1491 687 648 359 240 159
MspA1I CmG'CkG
Cuts at: 1606 2882 3042 3719 4867 5112 1606
Size: 1276 160 677 1148 245 2120
Fragments arranged by size:
2120 1276 1148 677 245 160
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4135
Fragments arranged by size:
4135 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NruI TCG'CGA
Cuts at: 2478 2478
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2984 4529 1132
Size: 97 1755 1545 2229
Fragments arranged by size:
2229 1755 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2044 1108
Size: 936 4690
PfoI T'CCnGG_A
Cuts at: 2160 2160
Size: 5626
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2676 2832 2978 830
Size: 1846 156 146 3478
Fragments arranged by size:
3478 1846 156 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
PvuII CAG'CTG
Cuts at: 2882 2882
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2672 2828 2974 4109 4790 4981 826
Size: 219 1627 156 146 1135 681 191 1471
Fragments arranged by size:
1627 1471 1135 681 219 191 156 146
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3730 4631 4893 820
Size: 197 926 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 2752 3550 5
Size: 47 2700 798 2081
Fragments arranged by size:
2700 2081 798 47
StuI AGG'CCT
Cuts at: 1517 2811 1517
Size: 1294 4332
StyI C'CwwG_G
Cuts at: 513 1065 2004 2610 3065 513
Size: 552 939 606 455 3074
Fragments arranged by size:
3074 939 606 552 455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2362 3653 3670 3823 4008 4453 325
Size: 1255 782 1291 17 153 185 445 1498
Fragments arranged by size:
1498 1291 1255 782 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2680 3872 5584
Size: 274 80 33 51 1443 703 1192 1712
Cuts at: 5584 96
Size: 138
Fragments arranged by size:
1712 1443 1192 703 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2202 2202
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 3755 3755
Size: 5626
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI