Restriction map for
pFN21AE0537
(Circular) (Six-base) MAPSORT of: pFN21AE0537.seq Check: 2278 from: 1 to: 5491
pFN21AE0537 5491 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2577 3301 278
Size: 53 83 186 936 1041 724 2468
Fragments arranged by size:
2468 1041 936 724 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2834 1937
Size: 897 4594
Acc65I G'GTAC_C
Cuts at: 2812 2812
Size: 5491
AclI AA'CG_TT
Cuts at: 1563 3618 3991 1563
Size: 2055 373 3063
Fragments arranged by size:
3063 2055 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3554 4938 5206 1632
Size: 1922 1384 268 1917
Fragments arranged by size:
1922 1917 1384 268
AfeI AGC'GCT
Cuts at: 2600 2600
Size: 5491
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5294
AflIII A'CryG_T
Cuts at: 1203 4390 5367 1203
Size: 3187 977 1327
Fragments arranged by size:
3187 1327 977
AgeI A'CCGG_T
Cuts at: 4322 4322
Size: 5491
AhdI GACnn_n'nnGTC
Cuts at: 4220 4220
Size: 5491
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5491
AlwNI CAG_nnn'CTG
Cuts at: 3234 4806 3234
Size: 1572 3919
ApaLI G'TGCA_C
Cuts at: 3548 4704 3548
Size: 1156 4335
ApoI r'AATT_y
Cuts at: 1493 2800 3026 3178 5287 1493
Size: 1307 226 152 2109 1697
Fragments arranged by size:
2109 1697 1307 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4045 160
Size: 872 3013 1606
Fragments arranged by size:
3013 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
AvaI C'yCGr_G
Cuts at: 1870 1943 2550 2582 2816 1870
Size: 73 607 32 234 4545
Fragments arranged by size:
4545 607 234 73 32
BamHI G'GATC_C
Cuts at: 2821 3188 2821
Size: 367 5124
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2812 4267 618
Size: 325 217 627 1025 1455 1842
Fragments arranged by size:
1842 1455 1025 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2810 1391
Size: 1419 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2569 928
Size: 971 670 3850
Fragments arranged by size:
3850 971 670
BbvCI CC'TCA_GC
Cuts at: 2342 2540 2342
Size: 198 5293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3682 3716 1903
Size: 34 1745 34 3678
Fragments arranged by size:
3678 1745 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3383 4593 3383
Size: 1210 4281
BclI T'GATC_A
Cuts at: 1548 2328 2562 1548
Size: 780 234 4477
Fragments arranged by size:
4477 780 234
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4102 136
Size: 107 122 71 631 605 2430 1525
Fragments arranged by size:
2430 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2688 5486 2688
Size: 2798 2693
BlpI GC'TnA_GC
Cuts at: 2184 2908 2184
Size: 724 4767
Bme1580I G_kGCm'C
Cuts at: 1790 3552 4708 1790
Size: 1762 1156 2573
Fragments arranged by size:
2573 1762 1156
BmgBI CAC'GTC
Cuts at: 1318 2062 1318
Size: 744 4747
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3222 4180 442
Size: 416 53 83 399 24 1805 958 1753
Fragments arranged by size:
1805 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5491
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2565 2565
Size: 5491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 4151 1239
Size: 1226 1686 2579
Fragments arranged by size:
2579 1686 1226
Bpu10I CC'TnA_GC
Cuts at: 2342 2360 2540 2342
Size: 18 180 5293
Fragments arranged by size:
5293 180 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3616 4481 4779 3616
Size: 865 298 4328
Fragments arranged by size:
4328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4154 882
Size: 617 2655 2219
Fragments arranged by size:
2655 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3282 493
Size: 711 2078 2702
Fragments arranged by size:
2702 2078 711
BsaBI GATnn'nnATC
Cuts at: 5485 5485
Size: 5491
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2574 3298 3680 4315
Size: 53 83 186 936 1041 724 382 635
Cuts at: 4315 275
Size: 1451
Fragments arranged by size:
1451 1041 936 724 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2397 3924 4322 4596 4743 5154 2397
Size: 1527 398 274 147 411 2734
Fragments arranged by size:
2734 1527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5266
Fragments arranged by size:
5266 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 2462 2568 2721 1186
Size: 1138 138 106 153 3956
Fragments arranged by size:
3956 1138 153 138 106
BseYI C'CCAG_C
Cuts at: 2191 2589 4694 5190 2191
Size: 398 2105 496 2492
Fragments arranged by size:
2492 2105 496 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2273 2422 2765 1163
Size: 573 426 111 149 343 3889
Fragments arranged by size:
3889 573 426 343 149 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2431 2941 3702 4730 1540
Size: 275 186 430 510 761 1028 2301
Fragments arranged by size:
2301 1028 761 510 430 275 186
BsiHKAI G_wGCw'C
Cuts at: 3552 3637 4708 3552
Size: 85 1071 4335
Fragments arranged by size:
4335 1071 85
BsmI GAATG_Cn'
Cuts at: 3002 3095 3002
Size: 93 5398
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2810 3552 3637 4708 1391
Size: 399 1020 742 85 1071 2174
Fragments arranged by size:
2174 1071 1020 742 399 85
BspEI T'CCGG_A
Cuts at: 2397 5154 2397
Size: 2757 2734
BspHI T'CATG_A
Cuts at: 2782 3380 5110 2782
Size: 598 1730 3163
Fragments arranged by size:
3163 1730 598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2846 844
Size: 2002 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3378 1394
Size: 429 1555 3507
Fragments arranged by size:
3507 1555 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3986 4160 65
Size: 1183 2738 174 1396
Fragments arranged by size:
2738 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 4135 4322 1700
Size: 110 270 2055 187 2869
Fragments arranged by size:
2869 2055 270 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5491
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5491
BssSI C'ACGA_G
Cuts at: 3551 4563 3551
Size: 1012 4479
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5491
BstBI TT'CG_AA
Cuts at: 4295 4295
Size: 5491
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5491
BstXI CCAn_nnnn'nTGG
Cuts at: 2651 2651
Size: 5491
BstYI r'GATC_y
Cuts at: 1972 2487 2688 2821 3188 3573 3590 5042 5128
Size: 515 201 133 367 385 17 1452 86
Cuts at: 5128 5140 5486 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 515 385 367 346 201 133
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2103 2733 2103
Size: 630 4861
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4000
Fragments arranged by size:
4000 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2764 487
Size: 683 56 1538 3214
Fragments arranged by size:
3214 1538 683 56
BtsI GCAGTG_nn'
Cuts at: 3007 3801 3821 3007
Size: 794 20 4677
Fragments arranged by size:
4677 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2359 2394 529
Size: 35 1795 35 3626
Fragments arranged by size:
3626 1795 35 35
DraI TTT'AAA
Cuts at: 2795 2795
Size: 5491
DraIII CAC_nnn'GTG
Cuts at: 2722 2722
Size: 5491
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4498 809
Size: 507 3182 1802
Fragments arranged by size:
3182 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2428 2713
Size: 54 1425 50 275 18 279 319 285
Cuts at: 2713 2938 3827 5456 8
Size: 225 889 1629 43
Fragments arranged by size:
1629 1425 889 319 285 279 275 225
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2428 2938 1537
Size: 275 616 510 4090
Fragments arranged by size:
4090 616 510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3421 1353
Size: 2068 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4078 4452 4598 387
Size: 1064 138 2489 374 146 1280
Fragments arranged by size:
2489 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 3554 4151 4938 5206 1239
Size: 393 833 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 833 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 2621 2306
Size: 315 5176
EcoO109I rG'GnC_Cy
Cuts at: 2070 2070
Size: 5491
EcoRI G'AATT_C
Cuts at: 2800 3178 2800
Size: 378 5113
FspI TGC'GCA
Cuts at: 3997 3997
Size: 5491
HaeII r_GCGC'y
Cuts at: 1678 2602 4638 1678
Size: 924 2036 2531
Fragments arranged by size:
2531 2036 924
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2130 2162 2259 2291 2547
Size: 32 135 32 558 32 97 32 256
Cuts at: 2547 2579 4132 4164 4206 4238 1373
Size: 32 1553 32 42 32 2626
Fragments arranged by size:
2626 1553 558 256 135 97 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2835 3081 669
Size: 988 281 897 246 3079
Fragments arranged by size:
3079 988 897 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5491
HpaI GTT'AAC
Cuts at: 3081 3081
Size: 5491
KpnI G_GTAC'C
Cuts at: 2816 2816
Size: 5491
MfeI C'AATT_G
Cuts at: 3090 3090
Size: 5491
MluI A'CGCG_T
Cuts at: 5367 5367
Size: 5491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4605 4789 1305
Size: 3300 184 2007
Fragments arranged by size:
3300 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2715 10
Size: 54 1425 343 883 2786
Fragments arranged by size:
2786 1425 883 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3449 3808 3967 518
Size: 648 2283 359 159 2042
Fragments arranged by size:
2283 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2907 3584 4732 4977 1606
Size: 585 716 677 1148 245 2120
Fragments arranged by size:
2120 1148 716 677 585 245
NaeI GCC'GGC
Cuts at: 2082 2082
Size: 5491
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4000
Fragments arranged by size:
4000 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5491
NgoMIV G'CCGG_C
Cuts at: 2080 2080
Size: 5491
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5491
NotI GC'GGCC_GC
Cuts at: 2938 2938
Size: 5491
NspI r_CATG'y
Cuts at: 1132 1229 2410 2849 4394 1132
Size: 97 1181 439 1545 2229
Fragments arranged by size:
2229 1545 1181 439 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5491
PciI A'CATG_T
Cuts at: 4390 4390
Size: 5491
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2722 1108
Size: 1614 3877
PfoI T'CCnGG_A
Cuts at: 2490 2490
Size: 5491
PmeI GTTT'AAAC
Cuts at: 2795 2795
Size: 5491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 5073 5105 3560
Size: 32 1481 32 3946
Fragments arranged by size:
3946 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2070 2070
Size: 5491
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5491
PsiI TTA'TAA
Cuts at: 3061 3061
Size: 5491
PstI C_TGCA'G
Cuts at: 830 2124 2322 2843 830
Size: 1294 198 521 3478
Fragments arranged by size:
3478 1294 521 198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5491
PvuII CAG'CTG
Cuts at: 2191 2191
Size: 5491
SalI G'TCGA_C
Cuts at: 1936 2833 1936
Size: 897 4594
SanDI GG'GwC_CC
Cuts at: 2070 2070
Size: 5491
SbfI CC_TGCA'GG
Cuts at: 2843 2843
Size: 5491
ScaI AGT'ACT
Cuts at: 3739 5451 3739
Size: 1712 3779
SfcI C'TryA_G
Cuts at: 826 1045 2120 2318 2839 3974 4655 4846 826
Size: 219 1075 198 521 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 681 521 219 198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5491
SmaI CCC'GGG
Cuts at: 1872 2818 1872
Size: 946 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2550 3595 4496 4758 820
Size: 197 926 607 1045 901 262 1553
Fragments arranged by size:
1553 1045 926 901 607 262 197
SnaBI TAC'GTA
Cuts at: 493 3282 493
Size: 2789 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5491
SphI G_CATG'C
Cuts at: 1132 2410 2849 1132
Size: 1278 439 3774
Fragments arranged by size:
3774 1278 439
SspI AAT'ATT
Cuts at: 5 52 3415 5
Size: 47 3363 2081
Fragments arranged by size:
3363 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5491
StyI C'CwwG_G
Cuts at: 513 1065 2004 2930 513
Size: 552 939 926 3074
Fragments arranged by size:
3074 939 926 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 3518 3535 3688 3873 4318 325
Size: 1255 603 1335 17 153 185 445 1498
Fragments arranged by size:
1498 1335 1255 603 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3737 5449 96
Size: 274 80 33 51 1443 1760 1712 138
Fragments arranged by size:
1760 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5451 5483 5451
Size: 32 5459
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5491
XbaI T'CTAG_A
Cuts at: 2827 2827
Size: 5491
XhoI C'TCGA_G
Cuts at: 1943 2550 1943
Size: 607 4884
XmaI C'CCGG_G
Cuts at: 1870 2816 1870
Size: 946 4545
XmnI GAAnn'nnTTC
Cuts at: 3620 3620
Size: 5491
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2575 3299 276
Size: 53 83 186 936 1041 724 2468
Fragments arranged by size:
2468 1041 936 724 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI