Restriction map for
pFN21AE0535
(Circular) (Six-base) MAPSORT of: pFN21AE0535.seq Check: 8980 from: 1 to: 5932
pFN21AE0535 5932 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2590 2590
Size: 5932
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2015 3742 278
Size: 53 83 186 936 479 1727 2468
Fragments arranged by size:
2468 1727 936 479 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2421 3275 1937
Size: 484 854 4594
Fragments arranged by size:
4594 854 484
Acc65I G'GTAC_C
Cuts at: 2241 3253 2241
Size: 1012 4920
AclI AA'CG_TT
Cuts at: 1563 4059 4432 1563
Size: 2496 373 3063
Fragments arranged by size:
3063 2496 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2543 3043 3995 5379 5647 1632
Size: 911 500 952 1384 268 1917
Fragments arranged by size:
1917 1384 952 911 500 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5735
AflIII A'CryG_T
Cuts at: 1203 4831 5808 1203
Size: 3628 977 1327
Fragments arranged by size:
3628 1327 977
AgeI A'CCGG_T
Cuts at: 4763 4763
Size: 5932
AhdI GACnn_n'nnGTC
Cuts at: 4661 4661
Size: 5932
AleI CACnn'nnGTG
Cuts at: 1166 2978 3143 1166
Size: 1812 165 3955
Fragments arranged by size:
3955 1812 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5932
AlwNI CAG_nnn'CTG
Cuts at: 3675 5247 3675
Size: 1572 4360
ApaI G_GGCC'C
Cuts at: 2666 2666
Size: 5932
ApaLI G'TGCA_C
Cuts at: 2674 3989 5145 2674
Size: 1315 1156 3461
Fragments arranged by size:
3461 1315 1156
ApoI r'AATT_y
Cuts at: 1493 3241 3467 3619 5728 1493
Size: 1748 226 152 2109 1697
Fragments arranged by size:
2109 1748 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4486 160
Size: 872 3454 1606
Fragments arranged by size:
3454 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
AvaI C'yCGr_G
Cuts at: 1870 1943 2739 3257 1870
Size: 73 796 518 4545
Fragments arranged by size:
4545 796 518 73
BamHI G'GATC_C
Cuts at: 2583 3262 3629 2583
Size: 679 367 4886
Fragments arranged by size:
4886 679 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 2241 2835 3253 4708
Size: 325 217 627 235 219 594 418 1455
Cuts at: 4708 618
Size: 1842
Fragments arranged by size:
1842 1455 627 594 418 325 235 219
217
BanII G_rGCy'C
Cuts at: 1391 2666 2757 2898 2987 3047 3137 3227 3251
Size: 1275 91 141 89 60 90 90 24
Cuts at: 3251 1391
Size: 4072
Fragments arranged by size:
4072 1275 141 91 90 90 89 60
24
BbeI G_GCGC'C
Cuts at: 2839 2839
Size: 5932
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4961
BbvCI CC'TCA_GC
Cuts at: 2498 2498
Size: 5932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4123 4157 1903
Size: 34 2186 34 3678
Fragments arranged by size:
3678 2186 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3824 5034 3824
Size: 1210 4722
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5932
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2404 4543 136
Size: 107 122 71 631 605 732 2139 1525
Fragments arranged by size:
2139 1525 732 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5927 5927
Size: 5932
BlpI GC'TnA_GC
Cuts at: 3349 3349
Size: 5932
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2666 2678 3993 5149 1790
Size: 235 641 12 1315 1156 2573
Fragments arranged by size:
2573 1315 1156 641 235 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5932
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2329 3663 4621
Size: 416 53 83 399 24 912 1334 958
Cuts at: 4621 442
Size: 1753
Fragments arranged by size:
1753 1334 958 912 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 2669 4592 1239
Size: 1058 372 1923 2579
Fragments arranged by size:
2579 1923 1058 372
Bpu10I CC'TnA_GC
Cuts at: 2096 2498 2096
Size: 402 5530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4057 4922 5220 4057
Size: 865 298 4769
Fragments arranged by size:
4769 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4595 882
Size: 617 3096 2219
Fragments arranged by size:
3096 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3723 493
Size: 711 2519 2702
Fragments arranged by size:
2702 2519 711
BsaBI GATnn'nnATC
Cuts at: 5926 5926
Size: 5932
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2836 3090 3739
Size: 53 83 186 936 479 824 254 649
Cuts at: 3739 4121 4756 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 824 649 635 479 382 254
186 83 53
BsaWI w'CCGG_w
Cuts at: 4365 4763 5037 5184 5595 4365
Size: 398 274 147 411 4702
Fragments arranged by size:
4702 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2969 2999 3119 3149 1178
Size: 30 165 30 1566 30 120 30 3961
Fragments arranged by size:
3961 1566 165 120 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5932
BseYI C'CCAG_C
Cuts at: 2284 5135 5631 2284
Size: 2851 496 2585
Fragments arranged by size:
2851 2585 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2027 2537 2999 1163
Size: 573 291 510 462 4096
Fragments arranged by size:
4096 573 510 462 291
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3382 4143 5171 1540
Size: 275 186 1381 761 1028 2301
Fragments arranged by size:
2301 1381 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2678 2757 2898 3114 3150 3993 4078 5149 2678
Size: 79 141 216 36 843 85 1071 3461
Fragments arranged by size:
3461 1071 843 216 141 85 79 36
BsmI GAATG_Cn'
Cuts at: 3443 3536 3443
Size: 93 5839
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2666 2678 2757 2898 2987 3047
Size: 399 235 641 12 79 141 89 60
Cuts at: 3047 3114 3137 3150 3227 3251 3993 4078 5149
Size: 67 23 13 77 24 742 85 1071
Cuts at: 5149 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 641 399 235 141 89
85 79 77 67 60 24 23 13
12
BspEI T'CCGG_A
Cuts at: 5595 5595
Size: 5932
BspHI T'CATG_A
Cuts at: 3821 5551 3821
Size: 1730 4202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 2590 2614 2694 3021 3287 844
Size: 1643 103 24 80 327 266 3489
Fragments arranged by size:
3489 1643 327 266 103 80 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2914 3819 1394
Size: 429 1091 905 3507
Fragments arranged by size:
3507 1091 905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4427 4601 65
Size: 1183 3179 174 1396
Fragments arranged by size:
3179 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2244 2832 4576 4763 1700
Size: 110 434 588 1744 187 2869
Fragments arranged by size:
2869 1744 588 434 187 110
BsrGI T'GTAC_A
Cuts at: 96 2334 2430 96
Size: 2238 96 3598
Fragments arranged by size:
3598 2238 96
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5932
BssSI C'ACGA_G
Cuts at: 3992 5004 3992
Size: 1012 4920
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5932
BstBI TT'CG_AA
Cuts at: 4736 4736
Size: 5932
BstEII G'GTnAC_C
Cuts at: 1184 2635 2734 1184
Size: 1451 99 4382
Fragments arranged by size:
4382 1451 99
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2980 2215
Size: 765 5167
BstYI r'GATC_y
Cuts at: 1972 2583 3262 3629 4014 4031 5483 5569 5581
Size: 611 679 367 385 17 1452 86 12
Cuts at: 5581 5927 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 679 611 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2057 513
Size: 552 939 12 41 4388
Fragments arranged by size:
4388 939 552 41 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5193
Fragments arranged by size:
5193 683 56
BtsI GCAGTG_nn'
Cuts at: 2088 2166 2794 3448 4242 4262 2088
Size: 78 628 654 794 20 3758
Fragments arranged by size:
3758 794 654 628 78 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5932
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5897
DraI TTT'AAA
Cuts at: 3236 3236
Size: 5932
DraIII CAC_nnn'GTG
Cuts at: 2560 2560
Size: 5932
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4939 809
Size: 507 3623 1802
Fragments arranged by size:
3623 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897
Size: 54 1425 50 275 18 1549 889 1629
Cuts at: 5897 8
Size: 43
Fragments arranged by size:
1629 1549 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3379 1537
Size: 275 1567 4090
Fragments arranged by size:
4090 1567 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2903 3862 1353
Size: 1550 959 3423
Fragments arranged by size:
3423 1550 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2077 4519 4893 5039 387
Size: 1064 138 488 2442 374 146 1280
Fragments arranged by size:
2442 1280 1064 488 374 146 138
EcoICRI GAG'CTC
Cuts at: 2755 2896 2755
Size: 141 5791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2543 2669 3043 3995 4592 5379
Size: 393 665 246 126 374 952 597 787
Cuts at: 5379 5647 1239
Size: 268 1524
Fragments arranged by size:
1524 952 787 665 597 393 374 268
246 126
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 2384
Size: 5932
EcoO109I rG'GnC_Cy
Cuts at: 2476 2683 3010 3160 2476
Size: 207 327 150 5248
Fragments arranged by size:
5248 327 207 150
EcoRI G'AATT_C
Cuts at: 3241 3619 3241
Size: 378 5554
FspI TGC'GCA
Cuts at: 2411 4438 2411
Size: 2027 3905
HaeII r_GCGC'y
Cuts at: 1678 2839 5079 1678
Size: 1161 2240 2531
Fragments arranged by size:
2531 2240 1161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3075 3107 3186 3218 4573
Size: 32 135 32 1503 32 79 32 1355
Cuts at: 4573 4605 4647 4679 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1503 1355 135 79 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3276 3522 669
Size: 988 281 1338 246 3079
Fragments arranged by size:
3079 1338 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5932
HpaI GTT'AAC
Cuts at: 3522 3522
Size: 5932
KasI G'GCGC_C
Cuts at: 2835 2835
Size: 5932
KpnI G_GTAC'C
Cuts at: 2245 3257 2245
Size: 1012 4920
MfeI C'AATT_G
Cuts at: 3531 3531
Size: 5932
MluI A'CGCG_T
Cuts at: 5808 5808
Size: 5932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5046 5230 1305
Size: 3741 184 2007
Fragments arranged by size:
3741 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4110
Fragments arranged by size:
4110 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2079 2978 3143 3890 4249 4408 518
Size: 648 913 899 165 747 359 159 2042
Fragments arranged by size:
2042 913 899 747 648 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 2468 2731 2963 3184 3348 4025 5173 5418
Size: 862 263 232 221 164 677 1148 245
Cuts at: 5418 1606
Size: 2120
Fragments arranged by size:
2120 1148 862 677 263 245 232 221
164
NarI GG'CG_CC
Cuts at: 2836 2836
Size: 5932
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4441
Fragments arranged by size:
4441 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5932
NotI GC'GGCC_GC
Cuts at: 3379 3379
Size: 5932
NspI r_CATG'y
Cuts at: 1132 1229 3290 4835 1132
Size: 97 2061 1545 2229
Fragments arranged by size:
2229 2061 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2783 3220 1348
Size: 1435 437 4060
Fragments arranged by size:
4060 1435 437
PciI A'CATG_T
Cuts at: 4831 4831
Size: 5932
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2576 2817 1108
Size: 1468 241 4223
Fragments arranged by size:
4223 1468 241
PmeI GTTT'AAAC
Cuts at: 3236 3236
Size: 5932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4001 4033 5514 5546 4001
Size: 32 1481 32 4387
Fragments arranged by size:
4387 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 2683 3010 2476
Size: 207 327 5398
Fragments arranged by size:
5398 327 207
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5932
PsiI TTA'TAA
Cuts at: 3502 3502
Size: 5932
PspOMI G'GGCC_C
Cuts at: 2662 2662
Size: 5932
PstI C_TGCA'G
Cuts at: 830 3284 830
Size: 2454 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5932
PvuII CAG'CTG
Cuts at: 2731 2963 3184 2731
Size: 232 221 5479
Fragments arranged by size:
5479 232 221
SacI G_AGCT'C
Cuts at: 2757 2898 2757
Size: 141 5791
SalI G'TCGA_C
Cuts at: 1936 3274 1936
Size: 1338 4594
SapI GCTCTTCn'nnn_
Cuts at: 2903 2903
Size: 5932
SbfI CC_TGCA'GG
Cuts at: 3284 3284
Size: 5932
ScaI AGT'ACT
Cuts at: 4180 5892 4180
Size: 1712 4220
SfcI C'TryA_G
Cuts at: 826 1045 2108 2422 3280 4415 5096 5287 826
Size: 219 1063 314 858 1135 681 191 1471
Fragments arranged by size:
1471 1135 1063 858 681 314 219 191
SfoI GGC'GCC
Cuts at: 2837 2837
Size: 5932
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
SgrAI Cr'CCGG_yG
Cuts at: 1700 2832 1700
Size: 1132 4800
SmaI CCC'GGG
Cuts at: 1872 2741 3259 1872
Size: 869 518 4545
Fragments arranged by size:
4545 869 518
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4036 4937 5199 820
Size: 197 926 2093 901 262 1553
Fragments arranged by size:
2093 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3723 493
Size: 3230 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5932
SphI G_CATG'C
Cuts at: 1132 3290 1132
Size: 2158 3774
SspI AAT'ATT
Cuts at: 5 52 3856 5
Size: 47 3804 2081
Fragments arranged by size:
3804 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5932
StyI C'CwwG_G
Cuts at: 513 1065 2004 3029 3165 3371 513
Size: 552 939 1025 136 206 3074
Fragments arranged by size:
3074 1025 939 552 206 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2317 3959 3976 4129 4314 4759 325
Size: 1255 737 1642 17 153 185 445 1498
Fragments arranged by size:
1642 1498 1255 737 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 2334 2430
Size: 274 80 33 51 1443 282 75 96
Cuts at: 2430 4178 5890 96
Size: 1748 1712 138
Fragments arranged by size:
1748 1712 1443 282 274 138 96 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5892 5924 5892
Size: 32 5900
Tth111I GACn'n_nGTC
Cuts at: 1316 2824 1316
Size: 1508 4424
XbaI T'CTAG_A
Cuts at: 3268 3268
Size: 5932
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5932
XmaI C'CCGG_G
Cuts at: 1870 2739 3257 1870
Size: 869 518 4545
Fragments arranged by size:
4545 869 518
XmnI GAAnn'nnTTC
Cuts at: 4061 4061
Size: 5932
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2013 3740 276
Size: 53 83 186 936 479 1727 2468
Fragments arranged by size:
2468 1727 936 479 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI