Restriction map for
pFN21AE0528
(Circular) (Six-base) MAPSORT of: pFN21AE0528.seq Check: 5079 from: 1 to: 5569
pFN21AE0528 5569 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2235 2912 1937
Size: 298 677 4594
Fragments arranged by size:
4594 677 298
Acc65I G'GTAC_C
Cuts at: 2890 2890
Size: 5569
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 3632 5016 5284 1632
Size: 443 1557 1384 268 1917
Fragments arranged by size:
1917 1557 1384 443 268
AfeI AGC'GCT
Cuts at: 2342 2342
Size: 5569
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2750 2760 1184
Size: 1566 10 3993
Fragments arranged by size:
3993 1566 10
AlwNI CAG_nnn'CTG
Cuts at: 2281 2662 2849 3312 4884 2281
Size: 381 187 463 1572 2966
Fragments arranged by size:
2966 1572 463 381 187
ApaI G_GGCC'C
Cuts at: 2301 2301
Size: 5569
ApaLI G'TGCA_C
Cuts at: 2075 3626 4782 2075
Size: 1551 1156 2862
Fragments arranged by size:
2862 1551 1156
ApoI r'AATT_y
Cuts at: 1493 2878 3104 3256 5365 1493
Size: 1385 226 152 2109 1697
Fragments arranged by size:
2109 1697 1385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2381 2894 1870
Size: 73 190 248 513 4545
Fragments arranged by size:
4545 513 248 190 73
BamHI G'GATC_C
Cuts at: 2899 3266 2899
Size: 367 5202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2701 2890 4345 618
Size: 325 217 627 914 189 1455 1842
Fragments arranged by size:
1842 1455 914 627 325 217 189
BanII G_rGCy'C
Cuts at: 1391 2219 2301 2382 2693 2888 1391
Size: 828 82 81 311 195 4072
Fragments arranged by size:
4072 828 311 195 82 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2215 928
Size: 971 316 4282
Fragments arranged by size:
4282 971 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3760 3794 1903
Size: 34 1823 34 3678
Fragments arranged by size:
3678 1823 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5569
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2305 4180 136
Size: 107 122 71 631 605 633 1875 1525
Fragments arranged by size:
1875 1525 633 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2986 2986
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2301 2704 3630 4786 1790
Size: 289 222 403 926 1156 2573
Fragments arranged by size:
2573 1156 926 403 289 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2213 3300 4258
Size: 416 53 83 399 24 796 1087 958
Cuts at: 4258 442
Size: 1753
Fragments arranged by size:
1753 1087 958 796 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2291 2591 4229 1239
Size: 968 84 300 1638 2579
Fragments arranged by size:
2579 1638 968 300 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3694 4559 4857 3694
Size: 865 298 4406
Fragments arranged by size:
4406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4232 882
Size: 617 2733 2219
Fragments arranged by size:
2733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3360 493
Size: 711 2156 2702
Fragments arranged by size:
2702 2156 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3376 3758 4393 275
Size: 53 83 186 936 1843 382 635 1451
Fragments arranged by size:
1843 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4002 4400 4674 4821 5232 4002
Size: 398 274 147 411 4339
Fragments arranged by size:
4339 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2774 2804 1178
Size: 30 165 30 1371 30 3943
Fragments arranged by size:
3943 1371 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2207 2663 1186
Size: 1018 3 456 4092
Fragments arranged by size:
4092 1018 456 3
BseYI C'CCAG_C
Cuts at: 2477 4772 5268 2477
Size: 2295 496 2778
Fragments arranged by size:
2778 2295 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 1163
Size: 573 501 4495
Fragments arranged by size:
4495 573 501
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2445 3630 3715 4786 2079
Size: 366 1185 85 1071 2862
Fragments arranged by size:
2862 1185 1071 366 85
BsmI GAATG_Cn'
Cuts at: 2098 3080 3173 2098
Size: 982 93 4494
Fragments arranged by size:
4494 982 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2130 2130
Size: 5569
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2219 2301 2382 2445 2693 2704
Size: 399 289 140 82 81 63 248 11
Cuts at: 2704 2888 3630 3715 4786 1391
Size: 184 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 289 248 184 140
85 82 81 63 11
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 2103 3458 5188 2103
Size: 1355 1730 2484
Fragments arranged by size:
2484 1730 1355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2481 2924 844
Size: 1637 443 3489
Fragments arranged by size:
3489 1637 443
BsrBI CCG'CTC
Cuts at: 1394 1823 2553 2790 3456 1394
Size: 429 730 237 666 3507
Fragments arranged by size:
3507 730 666 429 237
BsrDI GCAATG_nn'
Cuts at: 65 1248 2517 4064 4238 65
Size: 1183 1269 1547 174 1396
Fragments arranged by size:
1547 1396 1269 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4213 4400 1700
Size: 110 2403 187 2869
Fragments arranged by size:
2869 2403 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 2360 3629 4641 2360
Size: 1269 1012 3288
Fragments arranged by size:
3288 1269 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2093 2662 794
Size: 1299 569 3701
Fragments arranged by size:
3701 1299 569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 2128 2420 1184
Size: 944 292 4333
Fragments arranged by size:
4333 944 292
BstYI r'GATC_y
Cuts at: 1972 2899 3266 3651 3668 5120 5206 5218 5564
Size: 927 367 385 17 1452 86 12 346
Cuts at: 5564 1972
Size: 1977
Fragments arranged by size:
1977 1452 927 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2751 2865 513
Size: 552 939 747 114 3217
Fragments arranged by size:
3217 939 747 552 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4830
Fragments arranged by size:
4830 683 56
BtsI GCAGTG_nn'
Cuts at: 3085 3879 3899 3085
Size: 794 20 4755
Fragments arranged by size:
4755 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DraIII CAC_nnn'GTG
Cuts at: 2365 2365
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3016 3905 5534
Size: 54 1425 50 275 18 1186 889 1629
Cuts at: 5534 8
Size: 43
Fragments arranged by size:
1629 1425 1186 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2241 2601 3499 1353
Size: 888 360 898 3423
Fragments arranged by size:
3423 898 888 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2207 2291 2591 3632 4229 5016
Size: 393 443 132 84 300 1041 597 787
Cuts at: 5016 5284 1239
Size: 268 1524
Fragments arranged by size:
1524 1041 787 597 443 393 300 268
132 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2189 2273 2057
Size: 132 84 5353
Fragments arranged by size:
5353 132 84
EcoO109I rG'GnC_Cy
Cuts at: 2050 2278 2298 2491 2815 2050
Size: 228 20 193 324 4804
Fragments arranged by size:
4804 324 228 193 20
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 2344 4716 1678
Size: 666 2372 2531
Fragments arranged by size:
2531 2372 666
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4210 4242 4284 4316 1373
Size: 32 135 32 2638 32 42 32 2626
Fragments arranged by size:
2638 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3159 669
Size: 988 281 975 246 3079
Fragments arranged by size:
3079 988 975 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KpnI G_GTAC'C
Cuts at: 2894 2894
Size: 5569
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4683 4867 1305
Size: 3378 184 2007
Fragments arranged by size:
3378 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3747
Fragments arranged by size:
3747 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 2177 3527 3886 4045 518
Size: 648 1009 2 1350 359 159 2042
Fragments arranged by size:
2042 1350 1009 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2304 2719 2985 3662 4810 5055 1606
Size: 698 415 266 677 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 415 266 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2865 513
Size: 552 939 861 3217
Fragments arranged by size:
3217 939 861 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2032 2927 4472 1132
Size: 97 803 895 1545 2229
Fragments arranged by size:
2229 1545 895 803 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2365 1108
Size: 1257 4312
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 2815 2278
Size: 537 5032
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PspOMI G'GGCC_C
Cuts at: 2297 2297
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2921 830
Size: 2091 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SapI GCTCTTCn'nnn_
Cuts at: 2241 2241
Size: 5569
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2850 2917 4052 4733 4924 826
Size: 219 1805 67 1135 681 191 1471
Fragments arranged by size:
1805 1471 1135 681 219 191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3673 4574 4836 820
Size: 197 926 1730 901 262 1553
Fragments arranged by size:
1730 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2032 2927 1132
Size: 900 895 3774
Fragments arranged by size:
3774 900 895
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 2679 2865 3008 513
Size: 552 939 63 612 186 143 3074
Fragments arranged by size:
3074 939 612 552 186 143 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 2408 3596 3613 3766 3951 4396
Size: 1255 516 312 1188 17 153 185 445
Cuts at: 4396 325
Size: 1498
Fragments arranged by size:
1498 1255 1188 516 445 312 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3815 5527 96
Size: 274 80 33 51 1443 1838 1712 138
Fragments arranged by size:
1838 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5529 5561 5529
Size: 32 5537
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI