Restriction map for
pFN21AE0527
(Circular) (Six-base) MAPSORT of: pFN21AE0527.seq Check: 1490 from: 1 to: 5437
pFN21AE0527 5437 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2476 2476
Size: 5437
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2055 2338 2780 1937
Size: 118 283 442 4594
Fragments arranged by size:
4594 442 283 118
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3500 4884 5152 1632
Size: 1868 1384 268 1917
Fragments arranged by size:
1917 1868 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 2032 4166 2032
Size: 2134 3303
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2295 1184
Size: 1111 4326
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 2435
Size: 32 5405
AlwNI CAG_nnn'CTG
Cuts at: 2431 3180 4752 2431
Size: 749 1572 3116
Fragments arranged by size:
3116 1572 749
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2746 2972 3124 5233 1493
Size: 1253 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2387 2762 1870
Size: 73 444 375 4545
Fragments arranged by size:
4545 444 375 73
BamHI G'GATC_C
Cuts at: 2730 2767 3134 2730
Size: 37 367 5033
Fragments arranged by size:
5033 367 37
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2327 2383 2758 4213 618
Size: 325 217 627 540 56 375 1455 1842
Fragments arranged by size:
1842 1455 627 540 375 325 217 56
BanII G_rGCy'C
Cuts at: 1391 2756 1391
Size: 1365 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3628 3662 1903
Size: 34 1691 34 3678
Fragments arranged by size:
3678 1691 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3329 4539 2268
Size: 1061 1210 3166
Fragments arranged by size:
3166 1210 1061
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2705 4048 136
Size: 107 122 71 631 605 1033 1343 1525
Fragments arranged by size:
1525 1343 1033 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5432 5432
Size: 5437
BlpI GC'TnA_GC
Cuts at: 2613 2854 2613
Size: 241 5196
Bme1580I G_kGCm'C
Cuts at: 1790 2284 2388 3498 4654 1790
Size: 494 104 1110 1156 2573
Fragments arranged by size:
2573 1156 1110 494 104
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3168 4126 442
Size: 416 53 83 399 24 1751 958 1753
Fragments arranged by size:
1753 1751 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 2534 4097 1239
Size: 1223 72 1563 2579
Fragments arranged by size:
2579 1563 1223 72
Bpu10I CC'TnA_GC
Cuts at: 2225 2225
Size: 5437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2665 3562 4427 4725 2159
Size: 506 897 865 298 2871
Fragments arranged by size:
2871 897 865 506 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 882
Size: 617 2601 2219
Fragments arranged by size:
2601 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2243 3244 3626 4261
Size: 53 83 186 936 710 1001 382 635
Cuts at: 4261 275
Size: 1451
Fragments arranged by size:
1451 1001 936 710 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3870 4268 4542 4689 5100 3870
Size: 398 274 147 411 4207
Fragments arranged by size:
4207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 1178
Size: 30 165 30 1032 30 4150
Fragments arranged by size:
4150 1032 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 2531 1186
Size: 1219 126 4092
Fragments arranged by size:
4092 1219 126
BseYI C'CCAG_C
Cuts at: 2037 2042 4640 5136 2037
Size: 5 2598 496 2338
Fragments arranged by size:
2598 2338 496 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4864
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2887 3648 4676 1540
Size: 275 186 886 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3498 3583 4654 3498
Size: 85 1071 4281
Fragments arranged by size:
4281 1071 85
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2388 2756 3498 3583 4654 1391
Size: 399 494 104 368 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 399 368 104 85
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 2290 3326 5056 2290
Size: 1036 1730 2671
Fragments arranged by size:
2671 1730 1036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2277 2476 2792 844
Size: 1433 199 316 3489
Fragments arranged by size:
3489 1433 316 199
BsrBI CCG'CTC
Cuts at: 1394 1823 3324 1394
Size: 429 1501 3507
Fragments arranged by size:
3507 1501 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2441 3932 4106 65
Size: 1183 1193 1491 174 1396
Fragments arranged by size:
1491 1396 1193 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2530 4081 4268 1700
Size: 110 720 1551 187 2869
Fragments arranged by size:
2869 1551 720 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 2277 3497 4509 2277
Size: 1220 1012 3205
Fragments arranged by size:
3205 1220 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 2719 4241 2719
Size: 1522 3915
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5437
BstYI r'GATC_y
Cuts at: 1972 2730 2767 3134 3519 3536 4988 5074 5086
Size: 758 37 367 385 17 1452 86 12
Cuts at: 5086 5432 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 758 385 367 346 86 37
17 12
BstZ17I GTA'TAC
Cuts at: 2339 2339
Size: 5437
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 2323 2418 513
Size: 552 939 96 223 95 3532
Fragments arranged by size:
3532 939 552 223 96 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3747 3767 2953
Size: 794 20 4623
Fragments arranged by size:
4623 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4444 809
Size: 507 3128 1802
Fragments arranged by size:
3128 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2479 2564
Size: 54 1425 50 275 18 378 271 85
Cuts at: 2564 2664 2884 3773 5402 8
Size: 100 220 889 1629 43
Fragments arranged by size:
1629 1425 889 378 275 271 220 100
85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2884 1537
Size: 275 1072 4090
Fragments arranged by size:
4090 1072 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3367 1353
Size: 2014 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 2534 3500 4097 4884 5152 1239
Size: 393 830 72 966 597 787 268 1524
Fragments arranged by size:
1524 966 830 787 597 393 268 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2657 2657
Size: 5437
EcoO109I rG'GnC_Cy
Cuts at: 2428 2466 2428
Size: 38 5399
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 5437
FspI TGC'GCA
Cuts at: 2096 3943 2096
Size: 1847 3590
HaeII r_GCGC'y
Cuts at: 1678 4584 1678
Size: 2906 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 4078 4110 4152
Size: 32 135 32 446 32 2028 32 42
Cuts at: 4152 4184 1373
Size: 32 2626
Fragments arranged by size:
2626 2028 446 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2781 3027 669
Size: 988 281 843 246 3079
Fragments arranged by size:
3079 988 843 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4551 4735 1305
Size: 3246 184 2007
Fragments arranged by size:
3246 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2481 10
Size: 54 1425 343 649 2966
Fragments arranged by size:
2966 1425 649 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3395 3754 3913 518
Size: 648 2229 359 159 2042
Fragments arranged by size:
2229 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2853 3530 4678 4923 1606
Size: 1247 677 1148 245 2120
Fragments arranged by size:
2120 1247 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2418 513
Size: 552 939 414 3532
Fragments arranged by size:
3532 939 552 414
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2312 2795 4340 1132
Size: 97 1083 483 1545 2229
Fragments arranged by size:
2229 1545 1083 483 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5437
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2359 2372 1108
Size: 1251 13 4173
Fragments arranged by size:
4173 1251 13
PfoI T'CCnGG_A
Cuts at: 2652 2652
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2435 2467 3506 3538 5019 5051 2435
Size: 32 1039 32 1481 32 2821
Fragments arranged by size:
2821 1481 1039 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PstI C_TGCA'G
Cuts at: 830 2274 2789 830
Size: 1444 515 3478
Fragments arranged by size:
3478 1444 515
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SbfI CC_TGCA'GG
Cuts at: 2274 2789 2274
Size: 515 4922
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SexAI A'CCwGG_T
Cuts at: 2028 2028
Size: 5437
SfcI C'TryA_G
Cuts at: 826 1045 2270 2785 3920 4601 4792 826
Size: 219 1225 515 1135 681 191 1471
Fragments arranged by size:
1471 1225 1135 681 515 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2764 1872
Size: 892 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 2644 3541 4442 4704 820
Size: 197 926 231 470 897 901 262 1553
Fragments arranged by size:
1553 926 901 897 470 262 231 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2312 2795 1132
Size: 1180 483 3774
Fragments arranged by size:
3774 1180 483
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 2699 1517
Size: 1182 4255
StyI C'CwwG_G
Cuts at: 513 1065 2004 2418 2876 513
Size: 552 939 414 458 3074
Fragments arranged by size:
3074 939 552 458 414
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 2597 3464 3481 3634 3819 4264
Size: 1255 633 384 867 17 153 185 445
Cuts at: 4264 325
Size: 1498
Fragments arranged by size:
1498 1255 867 633 445 384 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3683 5395 96
Size: 274 80 33 51 1443 1706 1712 138
Fragments arranged by size:
1712 1706 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2762 1870
Size: 892 4545
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI