Restriction map for
pFN21AE0526
(Circular) (Six-base) MAPSORT of: pFN21AE0526.seq Check: 6355 from: 1 to: 5236
pFN21AE0526 5236 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2432 2432
Size: 5236
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3046 278
Size: 53 83 186 936 1510 2468
Fragments arranged by size:
2468 1510 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2579 1937
Size: 642 4594
Acc65I G'GTAC_C
Cuts at: 2557 2557
Size: 5236
AclI AA'CG_TT
Cuts at: 1563 3363 3736 1563
Size: 1800 373 3063
Fragments arranged by size:
3063 1800 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 3299 4683 4951 1632
Size: 881 786 1384 268 1917
Fragments arranged by size:
1917 1384 881 786 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5039
AflIII A'CryG_T
Cuts at: 1203 4135 5112 1203
Size: 2932 977 1327
Fragments arranged by size:
2932 1327 977
AgeI A'CCGG_T
Cuts at: 4067 4067
Size: 5236
AhdI GACnn_n'nnGTC
Cuts at: 3965 3965
Size: 5236
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5236
AlwNI CAG_nnn'CTG
Cuts at: 2106 2125 2484 2979 4551 2106
Size: 19 359 495 1572 2791
Fragments arranged by size:
2791 1572 495 359 19
ApaLI G'TGCA_C
Cuts at: 3293 4449 3293
Size: 1156 4080
ApoI r'AATT_y
Cuts at: 1493 2545 2771 2923 5032 1493
Size: 1052 226 152 2109 1697
Fragments arranged by size:
2109 1697 1052 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3790 160
Size: 872 2758 1606
Fragments arranged by size:
2758 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5236
AvaI C'yCGr_G
Cuts at: 1870 1943 2229 2360 2561 1870
Size: 73 286 131 201 4545
Fragments arranged by size:
4545 286 201 131 73
BamHI G'GATC_C
Cuts at: 2566 2933 2566
Size: 367 4869
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2557 4012 618
Size: 325 217 627 770 1455 1842
Fragments arranged by size:
1842 1455 770 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2162 2555 1391
Size: 771 393 4072
Fragments arranged by size:
4072 771 393
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4265
BbvCI CC'TCA_GC
Cuts at: 2424 2424
Size: 5236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3427 3461 1903
Size: 34 1490 34 3678
Fragments arranged by size:
3678 1490 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3128 4338 3128
Size: 1210 4026
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3847 136
Size: 107 122 71 631 605 2175 1525
Fragments arranged by size:
2175 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2184 2514 5231 2184
Size: 330 2717 2189
Fragments arranged by size:
2717 2189 330
BlpI GC'TnA_GC
Cuts at: 2653 2653
Size: 5236
Bme1580I G_kGCm'C
Cuts at: 1790 3297 4453 1790
Size: 1507 1156 2573
Fragments arranged by size:
2573 1507 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5236
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2266 2967 3925
Size: 416 53 83 399 24 849 701 958
Cuts at: 3925 442
Size: 1753
Fragments arranged by size:
1753 958 849 701 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5236
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2181 2181
Size: 5236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 2233 3896 1239
Size: 798 196 1663 2579
Fragments arranged by size:
2579 1663 798 196
Bpu10I CC'TnA_GC
Cuts at: 2424 2448 2424
Size: 24 5212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3361 4226 4524 3361
Size: 865 298 4073
Fragments arranged by size:
4073 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3899 882
Size: 617 2400 2219
Fragments arranged by size:
2400 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3027 493
Size: 711 1823 2702
Fragments arranged by size:
2702 1823 711
BsaBI GATnn'nnATC
Cuts at: 5230 5230
Size: 5236
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3043 3425 4060 275
Size: 53 83 186 936 1510 382 635 1451
Fragments arranged by size:
1510 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3669 4067 4341 4488 4899 3669
Size: 398 274 147 411 4006
Fragments arranged by size:
4006 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5011
Fragments arranged by size:
5011 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5236
BseYI C'CCAG_C
Cuts at: 2161 4439 4935 2161
Size: 2278 496 2462
Fragments arranged by size:
2462 2278 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2367 1163
Size: 573 631 4032
Fragments arranged by size:
4032 631 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2686 3447 4475 1540
Size: 275 186 685 761 1028 2301
Fragments arranged by size:
2301 1028 761 685 275 186
BsiHKAI G_wGCw'C
Cuts at: 2449 3297 3382 4453 2449
Size: 848 85 1071 3232
Fragments arranged by size:
3232 1071 848 85
BsmI GAATG_Cn'
Cuts at: 2747 2840 2747
Size: 93 5143
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2449 2555 3297 3382 4453 1391
Size: 399 372 287 106 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 287 106 85
BspEI T'CCGG_A
Cuts at: 4899 4899
Size: 5236
BspHI T'CATG_A
Cuts at: 3125 4855 3125
Size: 1730 3506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 2591 844
Size: 1588 159 3489
Fragments arranged by size:
3489 1588 159
BsrBI CCG'CTC
Cuts at: 1394 1823 2401 3123 1394
Size: 429 578 722 3507
Fragments arranged by size:
3507 722 578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3731 3905 65
Size: 1183 2483 174 1396
Fragments arranged by size:
2483 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3880 4067 1700
Size: 110 2070 187 2869
Fragments arranged by size:
2869 2070 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5236
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5236
BssSI C'ACGA_G
Cuts at: 3296 4308 3296
Size: 1012 4224
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5236
BstBI TT'CG_AA
Cuts at: 4040 4040
Size: 5236
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5236
BstYI r'GATC_y
Cuts at: 1972 2103 2111 2184 2514 2566 2933 3318 3335
Size: 131 8 73 330 52 367 385 17
Cuts at: 3335 4787 4873 4885 5231 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 330 131 86
73 52 17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4684
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4497
Fragments arranged by size:
4497 683 56
BtsI GCAGTG_nn'
Cuts at: 2752 3546 3566 2752
Size: 794 20 4422
Fragments arranged by size:
4422 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5201
DraI TTT'AAA
Cuts at: 2540 2540
Size: 5236
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4243 809
Size: 507 2927 1802
Fragments arranged by size:
2927 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2683 3572 5201
Size: 54 1425 50 275 18 853 889 1629
Cuts at: 5201 8
Size: 43
Fragments arranged by size:
1629 1425 889 853 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2683 1537
Size: 275 871 4090
Fragments arranged by size:
4090 871 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2014 3166 1353
Size: 661 1152 3423
Fragments arranged by size:
3423 1152 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3823 4197 4343 387
Size: 1064 138 2234 374 146 1280
Fragments arranged by size:
2234 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2233 2513 3299 3896 4683 4951
Size: 393 405 196 280 786 597 787 268
Cuts at: 4951 1239
Size: 1524
Fragments arranged by size:
1524 787 786 597 405 393 280 268
196
EcoRI G'AATT_C
Cuts at: 2545 2923 2545
Size: 378 4858
FspI TGC'GCA
Cuts at: 3742 3742
Size: 5236
HaeII r_GCGC'y
Cuts at: 1678 4383 1678
Size: 2705 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3877 3909 3951 3983 1373
Size: 32 135 32 2305 32 42 32 2626
Fragments arranged by size:
2626 2305 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2580 2826 669
Size: 988 281 642 246 3079
Fragments arranged by size:
3079 988 642 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5236
HpaI GTT'AAC
Cuts at: 2826 2826
Size: 5236
KpnI G_GTAC'C
Cuts at: 2561 2561
Size: 5236
MfeI C'AATT_G
Cuts at: 2835 2835
Size: 5236
MluI A'CGCG_T
Cuts at: 5112 5112
Size: 5236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4350 4534 1305
Size: 3045 184 2007
Fragments arranged by size:
3045 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3414
Fragments arranged by size:
3414 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3194 3553 3712 518
Size: 648 2028 359 159 2042
Fragments arranged by size:
2042 2028 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2334 2381 2652 3329 4477 4722 1606
Size: 728 47 271 677 1148 245 2120
Fragments arranged by size:
2120 1148 728 677 271 245 47
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4684
NdeI CA'TA_TG
Cuts at: 387 2090 387
Size: 1703 3533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5236
NotI GC'GGCC_GC
Cuts at: 2683 2683
Size: 5236
NspI r_CATG'y
Cuts at: 1132 1229 2594 4139 1132
Size: 97 1365 1545 2229
Fragments arranged by size:
2229 1545 1365 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5236
PciI A'CATG_T
Cuts at: 4135 4135
Size: 5236
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 1108
Size: 1329 3907
PfoI T'CCnGG_A
Cuts at: 2053 2053
Size: 5236
PmeI GTTT'AAAC
Cuts at: 2540 2540
Size: 5236
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 4818 4850 3305
Size: 32 1481 32 3691
Fragments arranged by size:
3691 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5236
PsiI TTA'TAA
Cuts at: 2806 2806
Size: 5236
PstI C_TGCA'G
Cuts at: 830 2339 2588 830
Size: 1509 249 3478
Fragments arranged by size:
3478 1509 249
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5236
PvuII CAG'CTG
Cuts at: 2334 2381 2334
Size: 47 5189
SalI G'TCGA_C
Cuts at: 1936 2578 1936
Size: 642 4594
SapI GCTCTTCn'nnn_
Cuts at: 2014 2014
Size: 5236
SbfI CC_TGCA'GG
Cuts at: 2588 2588
Size: 5236
ScaI AGT'ACT
Cuts at: 3484 5196 3484
Size: 1712 3524
SfcI C'TryA_G
Cuts at: 826 1045 2335 2584 3719 4400 4591 826
Size: 219 1290 249 1135 681 191 1471
Fragments arranged by size:
1471 1290 1135 681 249 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5236
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5236
SmaI CCC'GGG
Cuts at: 1872 2231 2362 2563 1872
Size: 359 131 201 4545
Fragments arranged by size:
4545 359 201 131
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3340 4241 4503 820
Size: 197 926 1397 901 262 1553
Fragments arranged by size:
1553 1397 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3027 493
Size: 2534 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5236
SphI G_CATG'C
Cuts at: 1132 2594 1132
Size: 1462 3774
SspI AAT'ATT
Cuts at: 5 52 3160 5
Size: 47 3108 2081
Fragments arranged by size:
3108 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5236
StyI C'CwwG_G
Cuts at: 513 1065 2431 2675 513
Size: 552 1366 244 3074
Fragments arranged by size:
3074 1366 552 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3263 3280 3433 3618 4063 325
Size: 1255 1683 17 153 185 445 1498
Fragments arranged by size:
1683 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 3482 5194
Size: 274 80 33 51 1443 294 1211 1712
Cuts at: 5194 96
Size: 138
Fragments arranged by size:
1712 1443 1211 294 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5196 5228 5196
Size: 32 5204
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5236
XbaI T'CTAG_A
Cuts at: 2572 2572
Size: 5236
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5236
XmaI C'CCGG_G
Cuts at: 1870 2229 2360 2561 1870
Size: 359 131 201 4545
Fragments arranged by size:
4545 359 201 131
XmnI GAAnn'nnTTC
Cuts at: 2516 3365 2516
Size: 849 4387
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3044 276
Size: 53 83 186 936 1510 2468
Fragments arranged by size:
2468 1510 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI