Restriction map for
pFN21AE0523
(Circular) (Six-base) MAPSORT of: pFN21AE0523.seq Check: 1249 from: 1 to: 5389
pFN21AE0523 5389 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3199 278
Size: 53 83 186 936 1663 2468
Fragments arranged by size:
2468 1663 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2732 1937
Size: 795 4594
Acc65I G'GTAC_C
Cuts at: 2710 2710
Size: 5389
AclI AA'CG_TT
Cuts at: 1563 3516 3889 1563
Size: 1953 373 3063
Fragments arranged by size:
3063 1953 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2571 3452 4836 5104 1632
Size: 939 881 1384 268 1917
Fragments arranged by size:
1917 1384 939 881 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5192
AflIII A'CryG_T
Cuts at: 1203 2398 4288 5265 1203
Size: 1195 1890 977 1327
Fragments arranged by size:
1890 1327 1195 977
AgeI A'CCGG_T
Cuts at: 2418 4220 2418
Size: 1802 3587
AhdI GACnn_n'nnGTC
Cuts at: 4118 4118
Size: 5389
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5389
AlwNI CAG_nnn'CTG
Cuts at: 3132 4704 3132
Size: 1572 3817
ApaLI G'TGCA_C
Cuts at: 3446 4602 3446
Size: 1156 4233
ApoI r'AATT_y
Cuts at: 1493 2240 2698 2924 3076 5185 1493
Size: 747 458 226 152 2109 1697
Fragments arranged by size:
2109 1697 747 458 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3943 160
Size: 872 2911 1606
Fragments arranged by size:
2911 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
AvaI C'yCGr_G
Cuts at: 1870 1943 2714 1870
Size: 73 771 4545
Fragments arranged by size:
4545 771 73
BamHI G'GATC_C
Cuts at: 2719 3086 2719
Size: 367 5022
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2710 4165 618
Size: 325 217 627 923 1455 1842
Fragments arranged by size:
1842 1455 923 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2708 1391
Size: 1317 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2268 928
Size: 971 369 4049
Fragments arranged by size:
4049 971 369
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3580 3614 1903
Size: 34 1643 34 3678
Fragments arranged by size:
3678 1643 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3281 4491 3281
Size: 1210 4179
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5389
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4000 136
Size: 107 122 71 631 605 2328 1525
Fragments arranged by size:
2328 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5384 5384
Size: 5389
BlpI GC'TnA_GC
Cuts at: 2806 2806
Size: 5389
Bme1580I G_kGCm'C
Cuts at: 1790 3450 4606 1790
Size: 1660 1156 2573
Fragments arranged by size:
2573 1660 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5389
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3120 4078 442
Size: 416 53 83 399 24 1703 958 1753
Fragments arranged by size:
1753 1703 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2675 4049 1239
Size: 1436 1374 2579
Fragments arranged by size:
2579 1436 1374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3514 4379 4677 3514
Size: 865 298 4226
Fragments arranged by size:
4226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4052 882
Size: 617 2553 2219
Fragments arranged by size:
2553 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2399 3180 493
Size: 711 1195 781 2702
Fragments arranged by size:
2702 1195 781 711
BsaBI GATnn'nnATC
Cuts at: 5383 5383
Size: 5389
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3196 3578 4213 275
Size: 53 83 186 936 1663 382 635 1451
Fragments arranged by size:
1663 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2418 3822 4220 4494 4641 5052 2418
Size: 1404 398 274 147 411 2755
Fragments arranged by size:
2755 1404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5164
Fragments arranged by size:
5164 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5389
BseYI C'CCAG_C
Cuts at: 4592 5088 4592
Size: 496 4893
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4816
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2839 3600 4628 1540
Size: 275 186 838 761 1028 2301
Fragments arranged by size:
2301 1028 838 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3450 3535 4606 3450
Size: 85 1071 4233
Fragments arranged by size:
4233 1071 85
BsmI GAATG_Cn'
Cuts at: 2900 2993 2900
Size: 93 5296
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2708 3450 3535 4606 1391
Size: 399 918 742 85 1071 2174
Fragments arranged by size:
2174 1071 918 742 399 85
BspEI T'CCGG_A
Cuts at: 5052 5052
Size: 5389
BspHI T'CATG_A
Cuts at: 3278 5008 3278
Size: 1730 3659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 2744 844
Size: 1343 557 3489
Fragments arranged by size:
3489 1343 557
BsrBI CCG'CTC
Cuts at: 1394 1823 3276 1394
Size: 429 1453 3507
Fragments arranged by size:
3507 1453 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3884 4058 65
Size: 1183 2636 174 1396
Fragments arranged by size:
2636 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2418 4033 4220 1700
Size: 110 608 1615 187 2869
Fragments arranged by size:
2869 1615 608 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5389
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5389
BssSI C'ACGA_G
Cuts at: 3449 4461 3449
Size: 1012 4377
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5389
BstBI TT'CG_AA
Cuts at: 4193 4193
Size: 5389
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5389
BstYI r'GATC_y
Cuts at: 1972 2719 3086 3471 3488 4940 5026 5038 5384
Size: 747 367 385 17 1452 86 12 346
Cuts at: 5384 1972
Size: 1977
Fragments arranged by size:
1977 1452 747 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2220 2220
Size: 5389
BtgI C'CryG_G
Cuts at: 513 1065 2004 2163 513
Size: 552 939 159 3739
Fragments arranged by size:
3739 939 552 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4650
Fragments arranged by size:
4650 683 56
BtsI GCAGTG_nn'
Cuts at: 2905 3699 3719 2905
Size: 794 20 4575
Fragments arranged by size:
4575 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5354
DraI TTT'AAA
Cuts at: 2238 2693 2238
Size: 455 4934
DraIII CAC_nnn'GTG
Cuts at: 2345 2480 2345
Size: 135 5254
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4396 809
Size: 507 3080 1802
Fragments arranged by size:
3080 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2836 3725 5354
Size: 54 1425 50 275 18 1006 889 1629
Cuts at: 5354 8
Size: 43
Fragments arranged by size:
1629 1425 1006 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2836 1537
Size: 275 1024 4090
Fragments arranged by size:
4090 1024 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2667 3319 1353
Size: 1314 652 3423
Fragments arranged by size:
3423 1314 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3976 4350 4496 387
Size: 1064 138 2387 374 146 1280
Fragments arranged by size:
2387 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2571 2675 3452 4049 4836 5104 1239
Size: 393 939 104 777 597 787 268 1524
Fragments arranged by size:
1524 939 787 777 597 393 268 104
EcoO109I rG'GnC_Cy
Cuts at: 2213 2213
Size: 5389
EcoRI G'AATT_C
Cuts at: 2698 3076 2698
Size: 378 5011
FspI TGC'GCA
Cuts at: 3895 3895
Size: 5389
HaeII r_GCGC'y
Cuts at: 1678 4536 1678
Size: 2858 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4030 4062 4104 4136 1373
Size: 32 135 32 2458 32 42 32 2626
Fragments arranged by size:
2626 2458 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2733 2979 669
Size: 988 281 795 246 3079
Fragments arranged by size:
3079 988 795 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5389
HpaI GTT'AAC
Cuts at: 2979 2979
Size: 5389
KpnI G_GTAC'C
Cuts at: 2714 2714
Size: 5389
MfeI C'AATT_G
Cuts at: 2988 2988
Size: 5389
MluI A'CGCG_T
Cuts at: 5265 5265
Size: 5389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4503 4687 1305
Size: 3198 184 2007
Fragments arranged by size:
3198 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3567
Fragments arranged by size:
3567 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2589 3347 3706 3865 518
Size: 648 1423 758 359 159 2042
Fragments arranged by size:
2042 1423 758 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2805 3482 4630 4875 1606
Size: 1199 677 1148 245 2120
Fragments arranged by size:
2120 1199 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3898
Fragments arranged by size:
3898 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5389
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5389
NotI GC'GGCC_GC
Cuts at: 2836 2836
Size: 5389
NspI r_CATG'y
Cuts at: 1132 1229 2747 4292 1132
Size: 97 1518 1545 2229
Fragments arranged by size:
2229 1545 1518 97
PasI CC'CwG_GG
Cuts at: 1348 2052 1348
Size: 704 4685
PciI A'CATG_T
Cuts at: 4288 4288
Size: 5389
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5389
PmeI GTTT'AAAC
Cuts at: 2693 2693
Size: 5389
PmlI CAC'GTG
Cuts at: 2399 2399
Size: 5389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 4971 5003 3458
Size: 32 1481 32 3844
Fragments arranged by size:
3844 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2213 2213
Size: 5389
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5389
PsiI TTA'TAA
Cuts at: 2959 2959
Size: 5389
PstI C_TGCA'G
Cuts at: 830 2184 2741 830
Size: 1354 557 3478
Fragments arranged by size:
3478 1354 557
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5389
SalI G'TCGA_C
Cuts at: 1936 2731 1936
Size: 795 4594
SbfI CC_TGCA'GG
Cuts at: 2741 2741
Size: 5389
ScaI AGT'ACT
Cuts at: 3637 5349 3637
Size: 1712 3677
SfcI C'TryA_G
Cuts at: 826 1045 2133 2180 2737 3872 4553 4744 826
Size: 219 1088 47 557 1135 681 191 1471
Fragments arranged by size:
1471 1135 1088 681 557 219 191 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5389
SmaI CCC'GGG
Cuts at: 1872 2716 1872
Size: 844 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3493 4394 4656 820
Size: 197 926 1550 901 262 1553
Fragments arranged by size:
1553 1550 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3180 493
Size: 2687 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5389
SphI G_CATG'C
Cuts at: 1132 2747 1132
Size: 1615 3774
SspI AAT'ATT
Cuts at: 5 52 3313 5
Size: 47 3261 2081
Fragments arranged by size:
3261 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5389
StyI C'CwwG_G
Cuts at: 513 1065 2004 2828 513
Size: 552 939 824 3074
Fragments arranged by size:
3074 939 824 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3416 3433 3586 3771 4216 325
Size: 1255 1836 17 153 185 445 1498
Fragments arranged by size:
1836 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3635 5347 96
Size: 274 80 33 51 1443 1658 1712 138
Fragments arranged by size:
1712 1658 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2626 2658 5349 5381 2626
Size: 32 2691 32 2634
Fragments arranged by size:
2691 2634 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5389
XbaI T'CTAG_A
Cuts at: 2725 2725
Size: 5389
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5389
XmaI C'CCGG_G
Cuts at: 1870 2714 1870
Size: 844 4545
XmnI GAAnn'nnTTC
Cuts at: 2676 3518 2676
Size: 842 4547
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3197 276
Size: 53 83 186 936 1663 2468
Fragments arranged by size:
2468 1663 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI