Restriction map for
pFN21AE0520
(Circular) (Six-base) MAPSORT of: pFN21AE0520.seq Check: 767 from: 1 to: 5755
pFN21AE0520 5755 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2600 2600
Size: 5755
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2474 3818 5202 5470 1632
Size: 842 1344 1384 268 1917
Fragments arranged by size:
1917 1384 1344 842 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 2167 2879 1166
Size: 1001 712 4042
Fragments arranged by size:
4042 1001 712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2608 1184
Size: 1424 4331
AlwNI CAG_nnn'CTG
Cuts at: 2069 2691 3498 5070 2069
Size: 622 807 1572 2754
Fragments arranged by size:
2754 1572 807 622
ApaI G_GGCC'C
Cuts at: 2094 2146 2094
Size: 52 5703
ApaLI G'TGCA_C
Cuts at: 2222 3812 4968 2222
Size: 1590 1156 3009
Fragments arranged by size:
3009 1590 1156
ApoI r'AATT_y
Cuts at: 1493 3064 3290 3442 5551 1493
Size: 1571 226 152 2109 1697
Fragments arranged by size:
2109 1697 1571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2145 3080 1870
Size: 73 153 49 935 4545
Fragments arranged by size:
4545 935 153 73 49
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2301 3076 4531 618
Size: 325 217 627 514 775 1455 1842
Fragments arranged by size:
1842 1455 775 627 514 325 217
BanII G_rGCy'C
Cuts at: 1391 2094 2146 3074 1391
Size: 703 52 928 4072
Fragments arranged by size:
4072 928 703 52
BbeI G_GCGC'C
Cuts at: 2305 2305
Size: 5755
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4784
BbvCI CC'TCA_GC
Cuts at: 2047 2933 2047
Size: 886 4869
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 2456 3005
Size: 107 122 71 631 605 478 306 549
Cuts at: 3005 4366 136
Size: 1361 1525
Fragments arranged by size:
1525 1361 631 605 549 478 306 122
107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 2783 3172 2783
Size: 389 5366
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2146 2226 2299 2400 2629 2932 3816
Size: 304 52 80 73 101 229 303 884
Cuts at: 3816 4972 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 884 304 303 229 101 80
73 52
BmgBI CAC'GTC
Cuts at: 1318 2361 2860 1318
Size: 1043 499 4213
Fragments arranged by size:
4213 1043 499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2562 2741 4415 1239
Size: 1323 179 1674 2579
Fragments arranged by size:
2579 1674 1323 179
Bpu10I CC'TnA_GC
Cuts at: 2047 2079 2933 2047
Size: 32 854 4869
Fragments arranged by size:
4869 854 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2591 3880 4745 5043 2591
Size: 1289 865 298 3303
Fragments arranged by size:
3303 1289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2941 3546 493
Size: 711 1737 605 2702
Fragments arranged by size:
2702 1737 711 605
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2302 3562 3944 4579
Size: 53 83 186 936 769 1260 382 635
Cuts at: 4579 275
Size: 1451
Fragments arranged by size:
1451 1260 936 769 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2550 4188 4586 4860 5007 5418 2550
Size: 1638 398 274 147 411 2887
Fragments arranged by size:
2887 1638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 1186
Size: 1468 4287
BseYI C'CCAG_C
Cuts at: 2087 2467 2684 3041 4958 5454 2087
Size: 380 217 357 1917 496 2388
Fragments arranged by size:
2388 1917 496 380 357 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 2727 1163
Size: 573 507 484 4191
Fragments arranged by size:
4191 573 507 484
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2080 2226 3816 3901 4972 2028
Size: 52 146 1590 85 1071 2811
Fragments arranged by size:
2811 1590 1071 146 85 52
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2080 2094 2146 2226 2299 2400
Size: 399 238 52 14 52 80 73 101
Cuts at: 2400 2629 2932 3074 3816 3901 4972 1391
Size: 229 303 142 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 238 229 142
101 85 80 73 52 52 14
BspEI T'CCGG_A
Cuts at: 2550 5418 2550
Size: 2868 2887
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2600 3110 844
Size: 1756 510 3489
Fragments arranged by size:
3489 1756 510
BsrBI CCG'CTC
Cuts at: 1394 1823 2512 3642 1394
Size: 429 689 1130 3507
Fragments arranged by size:
3507 1130 689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2564 4399 4586 1700
Size: 110 754 1835 187 2869
Fragments arranged by size:
2869 1835 754 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2325 794
Size: 1531 4224
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 2656 1184
Size: 1472 4283
BstYI r'GATC_y
Cuts at: 1972 2211 3085 3452 3837 3854 5306 5392 5404
Size: 239 874 367 385 17 1452 86 12
Cuts at: 5404 5750 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 874 385 367 346 239 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2118 2387 2118
Size: 269 5486
BtgI C'CryG_G
Cuts at: 513 1065 2154 2364 2502 2599 2877 513
Size: 552 1089 210 138 97 278 3391
Fragments arranged by size:
3391 1089 552 278 210 138 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2932 487
Size: 683 56 1706 3310
Fragments arranged by size:
3310 1706 683 56
BtsI GCAGTG_nn'
Cuts at: 2745 3271 4065 4085 2745
Size: 526 794 20 4415
Fragments arranged by size:
4415 794 526 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2151 2496
Size: 54 1425 50 275 18 307 14 345
Cuts at: 2496 2814 3202 4091 5720 8
Size: 318 388 889 1629 43
Fragments arranged by size:
1629 1425 889 388 345 318 307 275
54 50 43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3685 1353
Size: 2332 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3043 4342 4716 4862 387
Size: 1064 138 1454 1299 374 146 1280
Fragments arranged by size:
1454 1299 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2474 2562 2741 3818 4415 5202 5470
Size: 393 842 88 179 1077 597 787 268
Cuts at: 5470 1239
Size: 1524
Fragments arranged by size:
1524 1077 842 787 597 393 268 179
88
EcoNI CCTnn'n_nnAGG
Cuts at: 2633 2633
Size: 5755
EcoO109I rG'GnC_Cy
Cuts at: 2091 2122 2091
Size: 31 5724
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 5755
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2305 2462 4902 1678
Size: 627 157 2440 2531
Fragments arranged by size:
2531 2440 627 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4396 4428 4470 4502 1373
Size: 32 135 32 2824 32 42 32 2626
Fragments arranged by size:
2824 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KasI G'GCGC_C
Cuts at: 2301 2301
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2153 2816 10
Size: 54 1425 343 321 663 2949
Fragments arranged by size:
2949 1425 663 343 321 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2031 2167 2267 2363 2879 3713 4072
Size: 648 865 136 100 96 516 834 359
Cuts at: 4072 4231 518
Size: 159 2042
Fragments arranged by size:
2042 865 834 648 516 359 159 136
100 96
MspA1I CmG'CkG
Cuts at: 1606 2013 2069 2601 2787 3171 3848 4996 5241
Size: 407 56 532 186 384 677 1148 245
Cuts at: 5241 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 532 407 384 245 186
56
NarI GG'CG_CC
Cuts at: 2302 2302
Size: 5755
NcoI C'CATG_G
Cuts at: 513 1065 2154 2364 2502 513
Size: 552 1089 210 138 3766
Fragments arranged by size:
3766 1089 552 210 138
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2194 2266 2286 3113 4658 1132
Size: 97 965 72 20 827 1545 2229
Fragments arranged by size:
2229 1545 965 827 97 72 20
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2863 1108
Size: 1755 4000
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2608 1613
Size: 995 4760
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspOMI G'GGCC_C
Cuts at: 2090 2142 2090
Size: 52 5703
PstI C_TGCA'G
Cuts at: 830 2534 2637 3107 830
Size: 1704 103 470 3478
Fragments arranged by size:
3478 1704 470 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2069 2787 2069
Size: 718 5037
SacII CC_GC'GG
Cuts at: 2602 2602
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2530 2633 3103 4238 4919 5110 826
Size: 219 1485 103 470 1135 681 191 1471
Fragments arranged by size:
1485 1471 1135 681 470 219 191 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 2150 2150
Size: 5755
SfoI GGC'GCC
Cuts at: 2303 2303
Size: 5755
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2606 3859 4760 5022 820
Size: 197 926 663 1253 901 262 1553
Fragments arranged by size:
1553 1253 926 901 663 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 2286 3113 1132
Size: 1154 827 3774
Fragments arranged by size:
3774 1154 827
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2154 2364 2502 3194 513
Size: 552 1089 210 138 692 3074
Fragments arranged by size:
3074 1089 692 552 210 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3782 3799 3952 4137 4582 325
Size: 1255 2202 17 153 185 445 1498
Fragments arranged by size:
2202 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2161 2161
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI
SwaI