(Circular) (Six-base) MAPSORT of: pFN21AE0519.seq Check: 7348 from: 1 to: 5281 pFN21AE0519 5281 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3091 278 Size: 53 83 186 936 1555 2468 Fragments arranged by size: 2468 1555 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2624 1937 Size: 687 4594 Acc65I G'GTAC_C Cuts at: 2602 2602 Size: 5281 AclI AA'CG_TT Cuts at: 1563 3408 3781 1563 Size: 1845 373 3063 Fragments arranged by size: 3063 1845 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3344 4728 4996 1632 Size: 1712 1384 268 1917 Fragments arranged by size: 1917 1712 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5084 AflIII A'CryG_T Cuts at: 1203 4180 5157 1203 Size: 2977 977 1327 Fragments arranged by size: 2977 1327 977 AgeI A'CCGG_T Cuts at: 4112 4112 Size: 5281 AhdI GACnn_n'nnGTC Cuts at: 4010 4010 Size: 5281 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5281 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2504 2536 2504 Size: 32 5249 AlwNI CAG_nnn'CTG Cuts at: 2524 3024 4596 2524 Size: 500 1572 3209 Fragments arranged by size: 3209 1572 500 ApaI G_GGCC'C Cuts at: 2022 2064 2022 Size: 42 5239 ApaLI G'TGCA_C Cuts at: 3338 4494 3338 Size: 1156 4125 ApoI r'AATT_y Cuts at: 1493 2590 2816 2968 5077 1493 Size: 1097 226 152 2109 1697 Fragments arranged by size: 2109 1697 1097 226 152 AseI AT'TA_AT Cuts at: 160 1032 3835 160 Size: 872 2803 1606 Fragments arranged by size: 2803 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5281 AvaI C'yCGr_G Cuts at: 1870 1943 2606 1870 Size: 73 663 4545 Fragments arranged by size: 4545 663 73 BamHI G'GATC_C Cuts at: 2611 2978 2611 Size: 367 4914 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2602 4057 618 Size: 325 217 627 815 1455 1842 Fragments arranged by size: 1842 1455 815 627 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2064 2600 1391 Size: 631 42 536 4072 Fragments arranged by size: 4072 631 536 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2324 928 Size: 971 425 3885 Fragments arranged by size: 3885 971 425 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3472 3506 1903 Size: 34 1535 34 3678 Fragments arranged by size: 3678 1535 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3173 4383 3173 Size: 1210 4071 BclI T'GATC_A Cuts at: 1548 2556 1548 Size: 1008 4273 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2166 3892 136 Size: 107 122 71 631 605 494 1726 1525 Fragments arranged by size: 1726 1525 631 605 494 122 107 71 BglII A'GATC_T Cuts at: 5276 5276 Size: 5281 BlpI GC'TnA_GC Cuts at: 2698 2698 Size: 5281 Bme1580I G_kGCm'C Cuts at: 1790 2022 2064 3342 4498 1790 Size: 232 42 1278 1156 2573 Fragments arranged by size: 2573 1278 1156 232 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5281 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2058 2304 2497 Size: 416 53 83 399 24 641 246 193 Cuts at: 2497 3012 3970 442 Size: 515 958 1753 Fragments arranged by size: 1753 958 641 515 416 399 246 193 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 2466 3941 1239 Size: 793 434 1475 2579 Fragments arranged by size: 2579 1475 793 434 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2319 3406 4271 4569 2319 Size: 1087 865 298 3031 Fragments arranged by size: 3031 1087 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2201 3944 882 Size: 617 702 1743 2219 Fragments arranged by size: 2219 1743 702 617 BsaAI yAC'GTr Cuts at: 493 1204 3072 493 Size: 711 1868 2702 Fragments arranged by size: 2702 1868 711 BsaBI GATnn'nnATC Cuts at: 5275 5275 Size: 5281 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3088 3470 4105 275 Size: 53 83 186 936 1555 382 635 1451 Fragments arranged by size: 1555 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3714 4112 4386 4533 4944 3714 Size: 398 274 147 411 4051 Fragments arranged by size: 4051 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5056 Fragments arranged by size: 5056 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2316 2469 1186 Size: 1130 153 3998 Fragments arranged by size: 3998 1130 153 BseYI C'CCAG_C Cuts at: 4484 4980 4484 Size: 496 4785 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2073 1163 Size: 573 337 4371 Fragments arranged by size: 4371 573 337 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2731 3492 4520 1540 Size: 275 186 730 761 1028 2301 Fragments arranged by size: 2301 1028 761 730 275 186 BsiHKAI G_wGCw'C Cuts at: 3342 3427 4498 3342 Size: 85 1071 4125 Fragments arranged by size: 4125 1071 85 BsmI GAATG_Cn' Cuts at: 2792 2885 2792 Size: 93 5188 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2064 2600 3342 3427 4498 1391 Size: 399 232 42 536 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 536 399 232 85 42 BspEI T'CCGG_A Cuts at: 4944 4944 Size: 5281 BspHI T'CATG_A Cuts at: 3170 4900 3170 Size: 1730 3551 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2636 844 Size: 1792 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2094 3168 1394 Size: 429 271 1074 3507 Fragments arranged by size: 3507 1074 429 271 BsrDI GCAATG_nn' Cuts at: 65 1248 2083 3776 3950 65 Size: 1183 835 1693 174 1396 Fragments arranged by size: 1693 1396 1183 835 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3925 4112 1700 Size: 110 2115 187 2869 Fragments arranged by size: 2869 2115 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5281 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5281 BssSI C'ACGA_G Cuts at: 3341 4353 3341 Size: 1012 4269 BstAPI GCAn_nnn'nTGC Cuts at: 794 2127 794 Size: 1333 3948 BstBI TT'CG_AA Cuts at: 4085 4085 Size: 5281 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5281 BstYI r'GATC_y Cuts at: 1972 2611 2978 3363 3380 4832 4918 4930 5276 Size: 639 367 385 17 1452 86 12 346 Cuts at: 5276 1972 Size: 1977 Fragments arranged by size: 1977 1452 639 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3790 Fragments arranged by size: 3790 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4542 Fragments arranged by size: 4542 683 56 BtsI GCAGTG_nn' Cuts at: 2797 3591 3611 2797 Size: 794 20 4467 Fragments arranged by size: 4467 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5281 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5246 DraI TTT'AAA Cuts at: 2585 2585 Size: 5281 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2229 4288 809 Size: 507 913 2059 1802 Fragments arranged by size: 2059 1802 913 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 2183 2728 Size: 54 1425 50 275 18 337 16 545 Cuts at: 2728 3617 5246 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 545 337 275 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2728 1537 Size: 275 916 4090 Fragments arranged by size: 4090 916 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2180 2540 3211 1353 Size: 827 360 671 3423 Fragments arranged by size: 3423 827 671 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3868 4242 4388 387 Size: 1064 138 2279 374 146 1280 Fragments arranged by size: 2279 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2466 3344 3941 4728 4996 1239 Size: 393 400 434 878 597 787 268 1524 Fragments arranged by size: 1524 878 787 597 434 400 393 268 EcoO109I rG'GnC_Cy Cuts at: 2018 2019 2060 2305 2018 Size: 1 41 245 4994 Fragments arranged by size: 4994 245 41 1 EcoRI G'AATT_C Cuts at: 2590 2968 2590 Size: 378 4903 FspI TGC'GCA Cuts at: 3787 3787 Size: 5281 HaeII r_GCGC'y Cuts at: 1678 2085 4428 1678 Size: 407 2343 2531 Fragments arranged by size: 2531 2343 407 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2250 2282 2311 2343 3922 Size: 32 135 32 678 32 29 32 1579 Cuts at: 3922 3954 3996 4028 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1579 678 135 42 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 2625 2871 669 Size: 988 281 687 246 3079 Fragments arranged by size: 3079 988 687 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5281 HpaI GTT'AAC Cuts at: 2871 2871 Size: 5281 KpnI G_GTAC'C Cuts at: 2606 2606 Size: 5281 MfeI C'AATT_G Cuts at: 2880 2880 Size: 5281 MluI A'CGCG_T Cuts at: 5157 5157 Size: 5281 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2184 4395 4579 1305 Size: 879 2211 184 2007 Fragments arranged by size: 2211 2007 879 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2169 10 Size: 54 1425 343 337 3122 Fragments arranged by size: 3122 1425 343 337 54 MslI CAynn'nnrTG Cuts at: 518 1166 3239 3598 3757 518 Size: 648 2073 359 159 2042 Fragments arranged by size: 2073 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2486 2697 3374 4522 4767 1606 Size: 880 211 677 1148 245 2120 Fragments arranged by size: 2120 1148 880 677 245 211 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3790 Fragments arranged by size: 3790 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5281 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5281 NotI GC'GGCC_GC Cuts at: 2728 2728 Size: 5281 NspI r_CATG'y Cuts at: 1132 1229 2639 4184 1132 Size: 97 1410 1545 2229 Fragments arranged by size: 2229 1545 1410 97 PasI CC'CwG_GG Cuts at: 1348 2492 1348 Size: 1144 4137 PciI A'CATG_T Cuts at: 4180 4180 Size: 5281 PflMI CCAn_nnn'nTGG Cuts at: 1108 2509 1108 Size: 1401 3880 PmeI GTTT'AAAC Cuts at: 2585 2585 Size: 5281 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3350 3382 4863 4895 3350 Size: 32 1481 32 3736 Fragments arranged by size: 3736 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 2305 Size: 5281 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5281 PsiI TTA'TAA Cuts at: 2851 2851 Size: 5281 PspOMI G'GGCC_C Cuts at: 2018 2060 2018 Size: 42 5239 PstI C_TGCA'G Cuts at: 830 2633 830 Size: 1803 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5281 PvuII CAG'CTG Cuts at: 2486 2486 Size: 5281 SalI G'TCGA_C Cuts at: 1936 2623 1936 Size: 687 4594 SbfI CC_TGCA'GG Cuts at: 2633 2633 Size: 5281 ScaI AGT'ACT Cuts at: 3529 5241 3529 Size: 1712 3569 SfcI C'TryA_G Cuts at: 826 1045 2629 3764 4445 4636 826 Size: 219 1584 1135 681 191 1471 Fragments arranged by size: 1584 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5281 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5281 SmaI CCC'GGG Cuts at: 1872 2608 1872 Size: 736 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2334 3385 4286 4548 820 Size: 197 926 391 1051 901 262 1553 Fragments arranged by size: 1553 1051 926 901 391 262 197 SnaBI TAC'GTA Cuts at: 493 3072 493 Size: 2579 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5281 SphI G_CATG'C Cuts at: 1132 2639 1132 Size: 1507 3774 SspI AAT'ATT Cuts at: 5 52 3205 5 Size: 47 3153 2081 Fragments arranged by size: 3153 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5281 StyI C'CwwG_G Cuts at: 513 1065 2004 2142 2720 513 Size: 552 939 138 578 3074 Fragments arranged by size: 3074 939 578 552 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3308 3325 3478 3663 4108 325 Size: 1255 1728 17 153 185 445 1498 Fragments arranged by size: 1728 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3527 5239 96 Size: 274 80 33 51 1443 1550 1712 138 Fragments arranged by size: 1712 1550 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2520 2552 5241 5273 2520 Size: 32 2689 32 2528 Fragments arranged by size: 2689 2528 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2264 1316 Size: 948 4333 XbaI T'CTAG_A Cuts at: 2617 2617 Size: 5281 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2411 2411 Size: 5281 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5281 XmaI C'CCGG_G Cuts at: 1870 2606 1870 Size: 736 4545 XmnI GAAnn'nnTTC Cuts at: 3410 3410 Size: 5281 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3089 276 Size: 53 83 186 936 1555 2468 Fragments arranged by size: 2468 1555 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI