Restriction map for
pFN21AE0506
(Circular) (Six-base) MAPSORT of: pFN21AE0506.seq Check: 8201 from: 1 to: 5692
pFN21AE0506 5692 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2113 3755 5139 5407 1632
Size: 481 1642 1384 268 1917
Fragments arranged by size:
1917 1642 1384 481 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 2244 2331 4591 5568 1203
Size: 1041 87 2260 977 1327
Fragments arranged by size:
2260 1327 1041 977 87
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 2259 1166
Size: 1093 4599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2392 2821 3435 5007 2392
Size: 429 614 1572 3077
Fragments arranged by size:
3077 1572 614 429
ApaI G_GGCC'C
Cuts at: 2514 2514
Size: 5692
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2779 2930 3017 1870
Size: 73 836 151 87 4545
Fragments arranged by size:
4545 836 151 87 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2926 3013 4468 618
Size: 325 217 627 1139 87 1455 1842
Fragments arranged by size:
1842 1455 1139 627 325 217 87
BanII G_rGCy'C
Cuts at: 1391 2514 2543 3011 1391
Size: 1123 29 468 4072
Fragments arranged by size:
4072 1123 468 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4721
BbvCI CC'TCA_GC
Cuts at: 2220 2220
Size: 5692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2935 2969 3883 3917 1903
Size: 34 998 34 914 34 3678
Fragments arranged by size:
3678 998 914 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 2042 3109 2042
Size: 1067 4625
Bme1580I G_kGCm'C
Cuts at: 1790 2514 2931 3753 4909 1790
Size: 724 417 822 1156 2573
Fragments arranged by size:
2573 1156 822 724 417
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2748 3423 4381
Size: 416 53 83 399 24 1331 675 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1331 958 675 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2948 4352 1239
Size: 1709 1404 2579
Fragments arranged by size:
2579 1709 1404
Bpu10I CC'TnA_GC
Cuts at: 2220 2220
Size: 5692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2066 2135 4355 882
Size: 617 567 69 2220 2219
Fragments arranged by size:
2220 2219 617 567 69
BsaAI yAC'GTr
Cuts at: 493 1204 2332 3483 493
Size: 711 1128 1151 2702
Fragments arranged by size:
2702 1151 1128 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2442 2472 1178
Size: 30 165 30 1039 30 4398
Fragments arranged by size:
4398 1039 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 2767 1186
Size: 1003 578 4111
Fragments arranged by size:
4111 1003 578
BseYI C'CCAG_C
Cuts at: 2279 2513 2669 4895 5391 2279
Size: 234 156 2226 496 2580
Fragments arranged by size:
2580 2226 496 234 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1997 2291 1163
Size: 573 261 294 4564
Fragments arranged by size:
4564 573 294 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 3753 3838 4909 2328
Size: 1425 85 1071 3111
Fragments arranged by size:
3111 1425 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
BsmBI CGTCTCn'nnnn_
Cuts at: 2256 2573 2256
Size: 317 5375
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2514 2543 2931 3011 3753 3838
Size: 399 538 186 29 388 80 742 85
Cuts at: 3838 4909 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 388 186 85
80 29
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2849 3047 844
Size: 2005 198 3489
Fragments arranged by size:
3489 2005 198
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 3579 1394
Size: 429 260 1496 3507
Fragments arranged by size:
3507 1496 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 2491 4187 4361 65
Size: 1183 1243 1696 174 1396
Fragments arranged by size:
1696 1396 1243 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2115 4336 4523 1700
Size: 110 305 2221 187 2869
Fragments arranged by size:
2869 2221 305 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2118 2855 794
Size: 1324 737 3631
Fragments arranged by size:
3631 1324 737
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 2284 1184
Size: 1100 4592
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2725 2233
Size: 492 5200
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2148 2148
Size: 5692
BtgI C'CryG_G
Cuts at: 513 1065 2004 2397 2520 513
Size: 552 939 393 123 3685
Fragments arranged by size:
3685 939 552 393 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2293 2578 487
Size: 683 56 1067 285 3601
Fragments arranged by size:
3601 1067 683 285 56
BtsI GCAGTG_nn'
Cuts at: 2274 3208 4002 4022 2274
Size: 934 794 20 3944
Fragments arranged by size:
3944 934 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2185 2996 2185
Size: 811 4881
DraIII CAC_nnn'GTG
Cuts at: 2032 2332 2032
Size: 300 5392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 3139 4028
Size: 54 1425 50 275 18 489 820 889
Cuts at: 4028 5657 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 820 489 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2102 2624 3622 1353
Size: 749 522 998 3423
Fragments arranged by size:
3423 998 749 522
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2463 4279 4653 4799 387
Size: 1064 138 874 1816 374 146 1280
Fragments arranged by size:
1816 1280 1064 874 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2113 2948 3755 4352 5139 5407 1239
Size: 393 481 835 807 597 787 268 1524
Fragments arranged by size:
1524 835 807 787 597 481 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2702 2702
Size: 5692
EcoO109I rG'GnC_Cy
Cuts at: 2389 2861 2959 2389
Size: 472 98 5122
Fragments arranged by size:
5122 472 98
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 5692
FspI TGC'GCA
Cuts at: 2804 4198 2804
Size: 1394 4298
FspAI rTGC'GCAy
Cuts at: 2804 2804
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2845 2877 4333 4365 4407
Size: 32 135 32 1273 32 1456 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1456 1273 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2259 2375 2723 3650 4009 4168
Size: 648 996 97 116 348 927 359 159
Cuts at: 4168 518
Size: 2042
Fragments arranged by size:
2042 996 927 648 359 348 159 116
97
MspA1I CmG'CkG
Cuts at: 1606 2259 2876 2968 3108 3785 4933 5178 1606
Size: 653 617 92 140 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 653 617 245 140 92
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4201
Fragments arranged by size:
4201 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 3050 4595 1132
Size: 97 1821 1545 2229
Fragments arranged by size:
2229 1821 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2821 1348
Size: 1473 4219
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2413 2923 1108
Size: 1305 510 3877
Fragments arranged by size:
3877 1305 510
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PmlI CAC'GTG
Cuts at: 2332 2332
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2389 2861 2959 2389
Size: 472 98 5122
Fragments arranged by size:
5122 472 98
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2510 2510
Size: 5692
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SapI GCTCTTCn'nnn_
Cuts at: 2102 2102
Size: 5692
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 3040 4175 4856 5047 826
Size: 219 1995 1135 681 191 1471
Fragments arranged by size:
1995 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 2982 1517
Size: 1465 4227
StyI C'CwwG_G
Cuts at: 513 1065 2004 2640 3131 513
Size: 552 939 636 491 3074
Fragments arranged by size:
3074 939 636 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2090 2293 3719 3736 3889 4074 4519
Size: 1255 510 203 1426 17 153 185 445
Cuts at: 4519 325
Size: 1498
Fragments arranged by size:
1498 1426 1255 510 445 203 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2442 2474 2512 2544 5652 5684 2442
Size: 32 38 32 3108 32 2450
Fragments arranged by size:
3108 2450 38 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 2552 2747 3821 2552
Size: 195 1074 4423
Fragments arranged by size:
4423 1074 195
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoICRI EcoRV FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI