Restriction map for
pFN21AE0503
(Circular) (Six-base) MAPSORT of: pFN21AE0503.seq Check: 1718 from: 1 to: 5365
pFN21AE0503 5365 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3175 278
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2708 1937
Size: 771 4594
Acc65I G'GTAC_C
Cuts at: 2686 2686
Size: 5365
AclI AA'CG_TT
Cuts at: 1563 3492 3865 1563
Size: 1929 373 3063
Fragments arranged by size:
3063 1929 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 3428 4812 5080 1632
Size: 701 1095 1384 268 1917
Fragments arranged by size:
1917 1384 1095 701 268
AfeI AGC'GCT
Cuts at: 2012 2129 2012
Size: 117 5248
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5168
AflIII A'CryG_T
Cuts at: 1203 4264 5241 1203
Size: 3061 977 1327
Fragments arranged by size:
3061 1327 977
AgeI A'CCGG_T
Cuts at: 4196 4196
Size: 5365
AhdI GACnn_n'nnGTC
Cuts at: 4094 4094
Size: 5365
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2640 1184
Size: 1456 3909
AlwNI CAG_nnn'CTG
Cuts at: 2077 2095 2251 2290 2521 3108 4680 2077
Size: 18 156 39 231 587 1572 2762
Fragments arranged by size:
2762 1572 587 231 156 39 18
ApaLI G'TGCA_C
Cuts at: 3422 4578 3422
Size: 1156 4209
ApoI r'AATT_y
Cuts at: 1493 2674 2900 3052 5161 1493
Size: 1181 226 152 2109 1697
Fragments arranged by size:
2109 1697 1181 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3919 160
Size: 872 2887 1606
Fragments arranged by size:
2887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
AvaI C'yCGr_G
Cuts at: 1870 1943 2690 1870
Size: 73 747 4545
Fragments arranged by size:
4545 747 73
BamHI G'GATC_C
Cuts at: 2391 2695 3062 2391
Size: 304 367 4694
Fragments arranged by size:
4694 367 304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2045 2177 2686 4141
Size: 325 217 627 234 24 132 509 1455
Cuts at: 4141 618
Size: 1842
Fragments arranged by size:
1842 1455 627 509 325 234 217 132
24
BanII G_rGCy'C
Cuts at: 1391 2684 1391
Size: 1293 4072
BbeI G_GCGC'C
Cuts at: 2025 2025
Size: 5365
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2167 2201 3556 3590 1903
Size: 34 230 34 1355 34 3678
Fragments arranged by size:
3678 1355 230 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3257 4467 3257
Size: 1210 4155
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 3976 136
Size: 107 122 71 631 605 646 1658 1525
Fragments arranged by size:
1658 1525 646 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5360 5360
Size: 5365
BlpI GC'TnA_GC
Cuts at: 2147 2358 2782 2147
Size: 211 424 4730
Fragments arranged by size:
4730 424 211
Bme1580I G_kGCm'C
Cuts at: 1790 2050 3426 4582 1790
Size: 260 1376 1156 2573
Fragments arranged by size:
2573 1376 1156 260
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2626 3096 4054
Size: 416 53 83 399 24 1209 470 958
Cuts at: 4054 442
Size: 1753
Fragments arranged by size:
1753 1209 958 470 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2453 2492 4025 1239
Size: 989 225 39 1533 2579
Fragments arranged by size:
2579 1533 989 225 39
Bpu10I CC'TnA_GC
Cuts at: 2279 2279
Size: 5365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3490 4355 4653 3490
Size: 865 298 4202
Fragments arranged by size:
4202 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4028 882
Size: 617 2529 2219
Fragments arranged by size:
2529 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2500 3156 493
Size: 711 1296 656 2702
Fragments arranged by size:
2702 1296 711 656
BsaBI GATnn'nnATC
Cuts at: 5359 5359
Size: 5365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 3172 3554 4189
Size: 53 83 186 936 489 1150 382 635
Cuts at: 4189 275
Size: 1451
Fragments arranged by size:
1451 1150 936 635 489 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2388 2442 2553 3798 4196 4470 4617 5028 2388
Size: 54 111 1245 398 274 147 411 2725
Fragments arranged by size:
2725 1245 411 398 274 147 111 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5140
Fragments arranged by size:
5140 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2414 1186
Size: 859 369 4137
Fragments arranged by size:
4137 859 369
BseYI C'CCAG_C
Cuts at: 2111 2313 2577 4568 5064 2111
Size: 202 264 1991 496 2412
Fragments arranged by size:
2412 1991 496 264 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2216 1163
Size: 573 480 4312
Fragments arranged by size:
4312 573 480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2815 3576 4604 1540
Size: 275 186 814 761 1028 2301
Fragments arranged by size:
2301 1028 814 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2280 2388 2468 3426 3511 4582 2280
Size: 108 80 958 85 1071 3063
Fragments arranged by size:
3063 1071 958 108 85 80
BsmI GAATG_Cn'
Cuts at: 2876 2969 2876
Size: 93 5272
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2280 2388 2468 2684 3426 3511
Size: 399 260 230 108 80 216 742 85
Cuts at: 3511 4582 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 260 230 216 108
85 80
BspEI T'CCGG_A
Cuts at: 2553 5028 2553
Size: 2475 2890
BspHI T'CATG_A
Cuts at: 3254 4984 3254
Size: 1730 3635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 2547 2720 844
Size: 1502 201 173 3489
Fragments arranged by size:
3489 1502 201 173
BsrBI CCG'CTC
Cuts at: 1394 1823 3252 1394
Size: 429 1429 3507
Fragments arranged by size:
3507 1429 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3860 4034 65
Size: 1183 2612 174 1396
Fragments arranged by size:
2612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2325 4009 4196 1700
Size: 110 515 1684 187 2869
Fragments arranged by size:
2869 1684 515 187 110
BsrGI T'GTAC_A
Cuts at: 96 2238 96
Size: 2142 3223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5365
BssSI C'ACGA_G
Cuts at: 3425 4437 3425
Size: 1012 4353
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2275 2290 794
Size: 1481 15 3869
Fragments arranged by size:
3869 1481 15
BstBI TT'CG_AA
Cuts at: 2187 4169 2187
Size: 1982 3383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5365
BstYI r'GATC_y
Cuts at: 1972 2211 2391 2695 3062 3447 3464 4916 5002
Size: 239 180 304 367 385 17 1452 86
Cuts at: 5002 5014 5360 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 304 239 180
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3874
Fragments arranged by size:
3874 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2426 487
Size: 683 56 1200 3426
Fragments arranged by size:
3426 1200 683 56
BtsI GCAGTG_nn'
Cuts at: 2881 3675 3695 2881
Size: 794 20 4551
Fragments arranged by size:
4551 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5330
DraI TTT'AAA
Cuts at: 2669 2669
Size: 5365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4372 809
Size: 507 3056 1802
Fragments arranged by size:
3056 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2812 3701 5330
Size: 54 1425 50 275 18 982 889 1629
Cuts at: 5330 8
Size: 43
Fragments arranged by size:
1629 1425 982 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2812 1537
Size: 275 1000 4090
Fragments arranged by size:
4090 1000 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3295 1353
Size: 1942 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 3952 4326 4472 387
Size: 1064 138 465 1898 374 146 1280
Fragments arranged by size:
1898 1280 1064 465 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2333 2453 2492 3428 4025 4812
Size: 393 596 105 120 39 936 597 787
Cuts at: 4812 5080 1239
Size: 268 1524
Fragments arranged by size:
1524 936 787 597 596 393 268 120
105 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2099
Size: 5365
EcoO109I rG'GnC_Cy
Cuts at: 2609 2609
Size: 5365
EcoRI G'AATT_C
Cuts at: 2674 3052 2674
Size: 378 4987
FspI TGC'GCA
Cuts at: 3871 3871
Size: 5365
HaeII r_GCGC'y
Cuts at: 1678 2014 2025 2131 2168 2251 2291 4512 1678
Size: 336 11 106 37 83 40 2221 2531
Fragments arranged by size:
2531 2221 336 106 83 40 37 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4006 4038 4080 4112 1373
Size: 32 135 32 2434 32 42 32 2626
Fragments arranged by size:
2626 2434 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2709 2955 669
Size: 988 281 771 246 3079
Fragments arranged by size:
3079 988 771 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5365
HpaI GTT'AAC
Cuts at: 2955 2955
Size: 5365
KasI G'GCGC_C
Cuts at: 2021 2021
Size: 5365
KpnI G_GTAC'C
Cuts at: 2690 2690
Size: 5365
MfeI C'AATT_G
Cuts at: 2964 2964
Size: 5365
MluI A'CGCG_T
Cuts at: 5241 5241
Size: 5365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4479 4663 1305
Size: 3174 184 2007
Fragments arranged by size:
3174 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3543
Fragments arranged by size:
3543 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3323 3682 3841 518
Size: 648 2157 359 159 2042
Fragments arranged by size:
2157 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2407 2781 3458 4606 4851 1606
Size: 468 333 374 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 468 374 333 245
NaeI GCC'GGC
Cuts at: 2327 2327
Size: 5365
NarI GG'CG_CC
Cuts at: 2022 2022
Size: 5365
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3874
Fragments arranged by size:
3874 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5365
NgoMIV G'CCGG_C
Cuts at: 2325 2325
Size: 5365
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5365
NotI GC'GGCC_GC
Cuts at: 2812 2812
Size: 5365
NspI r_CATG'y
Cuts at: 1132 1229 2723 4268 1132
Size: 97 1494 1545 2229
Fragments arranged by size:
2229 1545 1494 97
PasI CC'CwG_GG
Cuts at: 1348 2596 1348
Size: 1248 4117
PciI A'CATG_T
Cuts at: 4264 4264
Size: 5365
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5365
PfoI T'CCnGG_A
Cuts at: 2430 2430
Size: 5365
PmeI GTTT'AAAC
Cuts at: 2669 2669
Size: 5365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 4947 4979 3434
Size: 32 1481 32 3820
Fragments arranged by size:
3820 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2609 2609
Size: 5365
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5365
PsiI TTA'TAA
Cuts at: 2935 2935
Size: 5365
PstI C_TGCA'G
Cuts at: 830 2532 2565 2717 830
Size: 1702 33 152 3478
Fragments arranged by size:
3478 1702 152 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5365
PvuII CAG'CTG
Cuts at: 2074 2074
Size: 5365
SalI G'TCGA_C
Cuts at: 1936 2707 1936
Size: 771 4594
SbfI CC_TGCA'GG
Cuts at: 2717 2717
Size: 5365
ScaI AGT'ACT
Cuts at: 3613 5325 3613
Size: 1712 3653
SfcI C'TryA_G
Cuts at: 826 1045 2258 2528 2561 2713 3848 4529 4720
Size: 219 1213 270 33 152 1135 681 191
Cuts at: 4720 826
Size: 1471
Fragments arranged by size:
1471 1213 1135 681 270 219 191 152
33
SfoI GGC'GCC
Cuts at: 2023 2023
Size: 5365
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5365
SmaI CCC'GGG
Cuts at: 1872 2692 1872
Size: 820 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3469 4370 4632 820
Size: 197 926 1526 901 262 1553
Fragments arranged by size:
1553 1526 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3156 493
Size: 2663 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5365
SphI G_CATG'C
Cuts at: 1132 2723 1132
Size: 1591 3774
SspI AAT'ATT
Cuts at: 5 52 3289 5
Size: 47 3237 2081
Fragments arranged by size:
3237 2081 47
StuI AGG'CCT
Cuts at: 1517 2321 1517
Size: 804 4561
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 2804 513
Size: 552 939 21 779 3074
Fragments arranged by size:
3074 939 779 552 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3392 3409 3562 3747 4192 325
Size: 1255 1812 17 153 185 445 1498
Fragments arranged by size:
1812 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2238 3611 5323
Size: 274 80 33 51 1443 261 1373 1712
Cuts at: 5323 96
Size: 138
Fragments arranged by size:
1712 1443 1373 274 261 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5325 5357 5325
Size: 32 5333
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5365
XbaI T'CTAG_A
Cuts at: 2701 2701
Size: 5365
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5365
XmaI C'CCGG_G
Cuts at: 1870 2690 1870
Size: 820 4545
XmnI GAAnn'nnTTC
Cuts at: 3494 3494
Size: 5365
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3173 276
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI