Restriction map for
pFN21AE0500
(Circular) (Six-base) MAPSORT of: pFN21AE0500.seq Check: 414 from: 1 to: 6880
pFN21AE0500 6880 bp
With 182 enzymes: SgfI *
February 26, 2010 09:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3147 4690 278
Size: 53 83 186 936 1611 1543 2468
Fragments arranged by size:
2468 1611 1543 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2976 4223 1937
Size: 1039 1247 4594
Fragments arranged by size:
4594 1247 1039
Acc65I G'GTAC_C
Cuts at: 4201 4201
Size: 6880
AclI AA'CG_TT
Cuts at: 1563 5007 5380 1563
Size: 3444 373 3063
Fragments arranged by size:
3444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2009 3176 3593 4943 6327 6595 1632
Size: 377 1167 417 1350 1384 268 1917
Fragments arranged by size:
1917 1384 1350 1167 417 377 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6683
AflIII A'CryG_T
Cuts at: 1203 3417 5779 6756 1203
Size: 2214 2362 977 1327
Fragments arranged by size:
2362 2214 1327 977
AgeI A'CCGG_T
Cuts at: 5711 5711
Size: 6880
AhdI GACnn_n'nnGTC
Cuts at: 5609 5609
Size: 6880
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3405 1184
Size: 2221 4659
AlwNI CAG_nnn'CTG
Cuts at: 2477 3366 3646 4623 6195 2477
Size: 889 280 977 1572 3162
Fragments arranged by size:
3162 1572 977 889 280
ApaI G_GGCC'C
Cuts at: 3592 3786 3592
Size: 194 6686
ApaLI G'TGCA_C
Cuts at: 4937 6093 4937
Size: 1156 5724
ApoI r'AATT_y
Cuts at: 1493 2301 2696 2717 4189 4415 4567 6676 1493
Size: 808 395 21 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 808 395 226 152 21
AseI AT'TA_AT
Cuts at: 160 1032 5434 160
Size: 872 4402 1606
Fragments arranged by size:
4402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
AvaI C'yCGr_G
Cuts at: 1870 1943 3138 4205 1870
Size: 73 1195 1067 4545
Fragments arranged by size:
4545 1195 1067 73
BamHI G'GATC_C
Cuts at: 4210 4577 4210
Size: 367 6513
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 4201 5656 618
Size: 325 217 627 363 2051 1455 1842
Fragments arranged by size:
2051 1842 1455 627 363 325 217
BanII G_rGCy'C
Cuts at: 1391 2665 3592 3773 3786 3960 3999 4199 1391
Size: 1274 927 181 13 174 39 200 4072
Fragments arranged by size:
4072 1274 927 200 181 174 39 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2315 3139 3879 928
Size: 971 416 824 740 3929
Fragments arranged by size:
3929 971 824 740 416
BbvCI CC'TCA_GC
Cuts at: 3647 3647
Size: 6880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5071 5105 1903
Size: 34 3134 34 3678
Fragments arranged by size:
3678 3134 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4772 5982 4772
Size: 1210 5670
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6880
BfrBI ATG'CAT
Cuts at: 3110 3457 3110
Size: 347 6533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5491 136
Size: 107 122 71 631 605 3819 1525
Fragments arranged by size:
3819 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3213 3478 6875 3213
Size: 265 3397 3218
Fragments arranged by size:
3397 3218 265
BlpI GC'TnA_GC
Cuts at: 3767 4297 3767
Size: 530 6350
Bme1580I G_kGCm'C
Cuts at: 1790 3592 3786 4941 6097 1790
Size: 1802 194 1155 1156 2573
Fragments arranged by size:
2573 1802 1156 1155 194
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6880
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2701 3780 4611
Size: 416 53 83 399 24 1284 1079 831
Cuts at: 4611 5569 442
Size: 958 1753
Fragments arranged by size:
1753 1284 1079 958 831 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4149 1056
Size: 3093 3787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2936 3290 3956 4052 5540 1239
Size: 1697 354 666 96 1488 2579
Fragments arranged by size:
2579 1697 1488 666 354 96
Bpu10I CC'TnA_GC
Cuts at: 2376 2664 3647 2376
Size: 288 983 5609
Fragments arranged by size:
5609 983 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3944 4013 5005 5870 6168 3944
Size: 69 992 865 298 4656
Fragments arranged by size:
4656 992 865 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 3078 4028 5543 882
Size: 617 552 1027 950 1515 2219
Fragments arranged by size:
2219 1515 1027 950 617 552
BsaAI yAC'GTr
Cuts at: 493 1204 4671 493
Size: 711 3467 2702
Fragments arranged by size:
3467 2702 711
BsaBI GATnn'nnATC
Cuts at: 6874 6874
Size: 6880
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3144 4687 5069 5704
Size: 53 83 186 936 1611 1543 382 635
Cuts at: 5704 275
Size: 1451
Fragments arranged by size:
1611 1543 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3174 5313 5711 5985 6132 6543 3174
Size: 2139 398 274 147 411 3511
Fragments arranged by size:
3511 2139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3869 3899 1178
Size: 30 165 30 2466 30 4159
Fragments arranged by size:
4159 2466 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 2858 3890 3953 1186
Size: 1333 339 1032 63 4113
Fragments arranged by size:
4113 1333 1032 339 63
BseYI C'CCAG_C
Cuts at: 4068 6083 6579 4068
Size: 2015 496 4369
Fragments arranged by size:
4369 2015 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6307
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4330 5091 6119 1540
Size: 275 186 2329 761 1028 2301
Fragments arranged by size:
2329 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2665 2775 3960 3999 4941 5026 6097 2665
Size: 110 1185 39 942 85 1071 3448
Fragments arranged by size:
3448 1185 1071 942 110 85 39
BsmI GAATG_Cn'
Cuts at: 2927 3112 3459 4391 4484 2927
Size: 185 347 932 93 5323
Fragments arranged by size:
5323 932 347 185 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2665 2775 3592 3773 3786 3960 3999
Size: 399 875 110 817 181 13 174 39
Cuts at: 3999 4199 4941 5026 6097 1391
Size: 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 875 817 742 399 200 181
174 110 85 39 13
BspEI T'CCGG_A
Cuts at: 6543 6543
Size: 6880
BspHI T'CATG_A
Cuts at: 4769 6499 4769
Size: 1730 5150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3246 4235 844
Size: 2402 989 3489
Fragments arranged by size:
3489 2402 989
BsrBI CCG'CTC
Cuts at: 1394 1823 2334 3411 4767 1394
Size: 429 511 1077 1356 3507
Fragments arranged by size:
3507 1356 1077 511 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5375 5549 65
Size: 1183 4127 174 1396
Fragments arranged by size:
4127 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3522 5524 5711 1700
Size: 110 1712 2002 187 2869
Fragments arranged by size:
2869 2002 1712 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6880
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6880
BssSI C'ACGA_G
Cuts at: 4940 5952 4940
Size: 1012 5868
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3289 3366 794
Size: 2495 77 4308
Fragments arranged by size:
4308 2495 77
BstBI TT'CG_AA
Cuts at: 2326 2963 5684 2326
Size: 637 2721 3522
Fragments arranged by size:
3522 2721 637
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6880
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 4132 2011
Size: 2121 4759
BstYI r'GATC_y
Cuts at: 1972 2058 2285 3120 3213 3478 4210 4577 4962
Size: 86 227 835 93 265 732 367 385
Cuts at: 4962 4979 6431 6517 6529 6875 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 835 732 385 367 346 265
227 93 86 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 3129 513
Size: 552 939 6 1119 4264
Fragments arranged by size:
4264 1119 939 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6141
Fragments arranged by size:
6141 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 4396 5190 5210 2478
Size: 1918 794 20 4148
Fragments arranged by size:
4148 1918 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6845
DraI TTT'AAA
Cuts at: 3732 4184 3732
Size: 452 6428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5887 809
Size: 507 4571 1802
Fragments arranged by size:
4571 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2823 3046
Size: 54 1425 50 275 18 177 816 223
Cuts at: 3046 4122 4327 5216 6845 8
Size: 1076 205 889 1629 43
Fragments arranged by size:
1629 1425 1076 889 816 275 223 205
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4327 1537
Size: 275 2515 4090
Fragments arranged by size:
4090 2515 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 2548 2616 2651 2763 3813 3867 3948
Size: 1155 40 68 35 112 1050 54 81
Cuts at: 3948 4131 4810 1353
Size: 183 679 3423
Fragments arranged by size:
3423 1155 1050 679 183 112 81 68
54 40 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3096 5467 5841 5987 387
Size: 1064 138 1507 2371 374 146 1280
Fragments arranged by size:
2371 1507 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2663 3958 3997 2663
Size: 1295 39 5546
Fragments arranged by size:
5546 1295 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2009 2936 3176 3290 3593 3956 4052
Size: 393 377 927 240 114 303 363 96
Cuts at: 4052 4943 5540 6327 6595 1239
Size: 891 597 787 268 1524
Fragments arranged by size:
1524 927 891 787 597 393 377 363
303 268 240 114 96
EcoO109I rG'GnC_Cy
Cuts at: 3588 3589 3588
Size: 1 6879
EcoRI G'AATT_C
Cuts at: 4189 4567 4189
Size: 378 6502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2339 3580 2339
Size: 1241 5639
FspI TGC'GCA
Cuts at: 5386 5386
Size: 6880
HaeII r_GCGC'y
Cuts at: 1678 3366 3848 6027 1678
Size: 1688 482 2179 2531
Fragments arranged by size:
2531 2179 1688 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3869 3901 4089 4121 5521
Size: 32 135 32 2297 32 188 32 1400
Cuts at: 5521 5553 5595 5627 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2297 1400 188 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2293 3322 4224 4470 669
Size: 988 281 355 1029 902 246 3079
Fragments arranged by size:
3079 1029 988 902 355 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6880
HpaI GTT'AAC
Cuts at: 3322 4470 3322
Size: 1148 5732
KpnI G_GTAC'C
Cuts at: 4205 4205
Size: 6880
MfeI C'AATT_G
Cuts at: 4479 4479
Size: 6880
MluI A'CGCG_T
Cuts at: 3417 6756 3417
Size: 3339 3541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2086 5994 6178 1305
Size: 781 3908 184 2007
Fragments arranged by size:
3908 2007 781 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2825 3048 4124 10
Size: 54 1425 343 177 816 223 1076 2766
Fragments arranged by size:
2766 1425 1076 816 343 223 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2342 2393 3344 4838 5197 5356
Size: 648 843 333 51 951 1494 359 159
Cuts at: 5356 518
Size: 2042
Fragments arranged by size:
2042 1494 951 843 648 359 333 159
51
MspA1I CmG'CkG
Cuts at: 1606 2411 4132 4296 4973 6121 6366 1606
Size: 805 1721 164 677 1148 245 2120
Fragments arranged by size:
2120 1721 1148 805 677 245 164
NcoI C'CATG_G
Cuts at: 513 1065 2004 2010 3129 513
Size: 552 939 6 1119 4264
Fragments arranged by size:
4264 1119 939 552 6
NdeI CA'TA_TG
Cuts at: 387 3043 387
Size: 2656 4224
NheI G'CTAG_C
Cuts at: 1052 4145 1052
Size: 3093 3787
NotI GC'GGCC_GC
Cuts at: 4327 4327
Size: 6880
NsiI A_TGCA'T
Cuts at: 3112 3459 3112
Size: 347 6533
NspI r_CATG'y
Cuts at: 1132 1229 2347 4238 5783 1132
Size: 97 1118 1891 1545 2229
Fragments arranged by size:
2229 1891 1545 1118 97
PasI CC'CwG_GG
Cuts at: 1348 3592 1348
Size: 2244 4636
PciI A'CATG_T
Cuts at: 5779 5779
Size: 6880
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6880
PmeI GTTT'AAAC
Cuts at: 4184 4184
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4949 4981 6462 6494 4949
Size: 32 1481 32 5335
Fragments arranged by size:
5335 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6880
PsiI TTA'TAA
Cuts at: 4450 4450
Size: 6880
PspOMI G'GGCC_C
Cuts at: 3588 3782 3588
Size: 194 6686
PstI C_TGCA'G
Cuts at: 830 2410 2473 3309 3371 4232 830
Size: 1580 63 836 62 861 3478
Fragments arranged by size:
3478 1580 861 836 63 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6880
PvuII CAG'CTG
Cuts at: 2411 4132 2411
Size: 1721 5159
SacI G_AGCT'C
Cuts at: 2665 3960 3999 2665
Size: 1295 39 5546
Fragments arranged by size:
5546 1295 39
SalI G'TCGA_C
Cuts at: 1936 4222 1936
Size: 2286 4594
SapI GCTCTTCn'nnn_
Cuts at: 2508 2548 2763 3948 4131 2508
Size: 40 215 1185 183 5257
Fragments arranged by size:
5257 1185 215 183 40
SbfI CC_TGCA'GG
Cuts at: 4232 4232
Size: 6880
ScaI AGT'ACT
Cuts at: 5128 6840 5128
Size: 1712 5168
SfcI C'TryA_G
Cuts at: 826 1045 2406 2469 2977 3305 3367 4228 5363
Size: 219 1361 63 508 328 62 861 1135
Cuts at: 5363 6044 6235 826
Size: 681 191 1471
Fragments arranged by size:
1471 1361 1135 861 681 508 328 219
191 63 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6880
SmaI CCC'GGG
Cuts at: 1872 3140 4207 1872
Size: 1268 1067 4545
Fragments arranged by size:
4545 1268 1067
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3959 3992 4984 5885 6147 820
Size: 197 926 2016 33 992 901 262 1553
Fragments arranged by size:
2016 1553 992 926 901 262 197 33
SnaBI TAC'GTA
Cuts at: 493 4671 493
Size: 4178 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6880
SphI G_CATG'C
Cuts at: 1132 4238 1132
Size: 3106 3774
SspI AAT'ATT
Cuts at: 5 52 4804 5
Size: 47 4752 2081
Fragments arranged by size:
4752 2081 47
StuI AGG'CCT
Cuts at: 1517 2458 4112 1517
Size: 941 1654 4285
Fragments arranged by size:
4285 1654 941
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 3129 3867 4319 513
Size: 552 939 6 1119 738 452 3074
Fragments arranged by size:
3074 1119 939 738 552 452 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4025 4907 4924 5077 5262 5707 325
Size: 1255 2445 882 17 153 185 445 1498
Fragments arranged by size:
2445 1498 1255 882 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5126 6838 96
Size: 274 80 33 51 1443 3149 1712 138
Fragments arranged by size:
3149 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6840 6872 6840
Size: 32 6848
Tth111I GACn'n_nGTC
Cuts at: 1316 3755 1316
Size: 2439 4441
XbaI T'CTAG_A
Cuts at: 4216 4216
Size: 6880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2069 2893 3056 2069
Size: 824 163 5893
Fragments arranged by size:
5893 824 163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6880
XmaI C'CCGG_G
Cuts at: 1870 3138 4205 1870
Size: 1268 1067 4545
Fragments arranged by size:
4545 1268 1067
XmnI GAAnn'nnTTC
Cuts at: 2322 5009 2322
Size: 2687 4193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3145 4688 276
Size: 53 83 186 936 1611 1543 2468
Fragments arranged by size:
2468 1611 1543 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI