Restriction map for
pFN21AE0497
(Circular) (Six-base) MAPSORT of: pFN21AE0497.seq Check: 6687 from: 1 to: 5905
pFN21AE0497 5905 bp
With 182 enzymes: SgfI *
February 2, 2010 11:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2997 2997
Size: 5905
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2958 3715 278
Size: 53 83 186 936 1422 757 2468
Fragments arranged by size:
2468 1422 936 757 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2316 3248 1937
Size: 379 932 4594
Fragments arranged by size:
4594 932 379
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2237 2375 2837 2951 3968 5352 5620 1632
Size: 605 138 462 114 1017 1384 268 1917
Fragments arranged by size:
1917 1384 1017 605 462 268 138 114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 2571 4804 5781 1203
Size: 1368 2233 977 1327
Fragments arranged by size:
2233 1368 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 2685 2711 1166
Size: 1519 26 4360
Fragments arranged by size:
4360 1519 26
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2482 2749 3171 3648 5220 2482
Size: 267 422 477 1572 3167
Fragments arranged by size:
3167 1572 477 422 267
ApaI G_GGCC'C
Cuts at: 2369 2369
Size: 5905
ApaLI G'TGCA_C
Cuts at: 2714 3123 3962 5118 2714
Size: 409 839 1156 3501
Fragments arranged by size:
3501 1156 839 409
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
AvrII C'CTAG_G
Cuts at: 3148 3148
Size: 5905
BamHI G'GATC_C
Cuts at: 3016 3235 3602 3016
Size: 219 367 5319
Fragments arranged by size:
5319 367 219
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3226 4681 618
Size: 325 217 627 1439 1455 1842
Fragments arranged by size:
1842 1455 1439 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2369 2520 2562 3224 1391
Size: 978 151 42 662 4072
Fragments arranged by size:
4072 978 662 151 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3025 3059 4096 4130 1903
Size: 34 1088 34 1037 34 3678
Fragments arranged by size:
3678 1088 1037 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3131 3322 3131
Size: 191 5714
Bme1580I G_kGCm'C
Cuts at: 1790 2369 2718 3127 3966 5122 1790
Size: 579 349 409 839 1156 2573
Fragments arranged by size:
2573 1156 839 579 409 349
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2764 2929 3636
Size: 416 53 83 399 24 1347 165 707
Cuts at: 3636 4594 442
Size: 958 1753
Fragments arranged by size:
1753 1347 958 707 416 399 165 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4565 1239
Size: 3326 2579
Bpu10I CC'TnA_GC
Cuts at: 2423 2423
Size: 5905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2617 3696 493
Size: 711 1413 1079 2702
Fragments arranged by size:
2702 1413 1079 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2955 3712 4094 4729
Size: 53 83 186 936 1422 757 382 635
Cuts at: 4729 275
Size: 1451
Fragments arranged by size:
1451 1422 936 757 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2055 2292 4338 4736 5010 5157 5568 2055
Size: 237 2046 398 274 147 411 2392
Fragments arranged by size:
2392 2046 411 398 274 237 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2222 2252 1178
Size: 30 165 30 819 30 4831
Fragments arranged by size:
4831 819 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2156 2246 2267 2542 2957 3113 1186
Size: 898 72 90 21 275 415 156 3978
Fragments arranged by size:
3978 898 415 275 156 90 72 21
BseYI C'CCAG_C
Cuts at: 2694 2980 5108 5604 2694
Size: 286 2128 496 2995
Fragments arranged by size:
2995 2128 496 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 3106 3157 1163
Size: 573 771 599 51 3911
Fragments arranged by size:
3911 771 599 573 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2769 2876 3355 4116 5144 1540
Size: 275 186 768 107 479 761 1028 2301
Fragments arranged by size:
2301 1028 768 761 479 275 186 107
BsiHKAI G_wGCw'C
Cuts at: 2562 2718 3127 3966 4051 5122 2562
Size: 156 409 839 85 1071 3345
Fragments arranged by size:
3345 1071 839 409 156 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 2520 2562 2718 3127 3224 3966
Size: 399 579 151 42 156 409 97 742
Cuts at: 3966 4051 5122 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 409 399 156 151
97 85 42
BspEI T'CCGG_A
Cuts at: 2055 2292 5568 2055
Size: 237 3276 2392
Fragments arranged by size:
3276 2392 237
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2684 2904 2997 3260 844
Size: 1840 220 93 263 3489
Fragments arranged by size:
3489 1840 263 220 93
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 2050 3792 1394
Size: 429 205 22 1742 3507
Fragments arranged by size:
3507 1742 429 205 22
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 2199 2868 4549 4736 1700
Size: 110 338 51 669 1681 187 2869
Fragments arranged by size:
2869 1681 669 338 187 110 51
BsrGI T'GTAC_A
Cuts at: 96 2574 96
Size: 2478 3427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3171 794
Size: 2377 3528
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstXI CCAn_nnnn'nTGG
Cuts at: 2308 2687 2844 2308
Size: 379 157 5369
Fragments arranged by size:
5369 379 157
BstYI r'GATC_y
Cuts at: 1972 2991 3016 3235 3602 3987 4004 5456 5542
Size: 1019 25 219 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1019 385 367 346 219 86
25 17 12
Bsu36I CC'TnA_GG
Cuts at: 2111 2525 2111
Size: 414 5491
BtgI C'CryG_G
Cuts at: 513 1065 2004 2563 513
Size: 552 939 559 3855
Fragments arranged by size:
3855 939 559 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 2384 2643 2906 3421 4215 4235 2384
Size: 259 263 515 794 20 4054
Fragments arranged by size:
4054 794 515 263 259 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2280 2547
Size: 54 1425 50 275 18 327 123 267
Cuts at: 2547 2873 2973 3352 4241 5870 8
Size: 326 100 379 889 1629 43
Fragments arranged by size:
1629 1425 889 379 327 326 275 267
123 100 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2873 3352 1537
Size: 275 1061 479 4090
Fragments arranged by size:
4090 1061 479 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3835 1353
Size: 2482 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 2081 2141 4492 4866 5012
Size: 1064 138 471 21 60 2351 374 146
Cuts at: 5012 387
Size: 1280
Fragments arranged by size:
2351 1280 1064 471 374 146 138 60
21
EcoICRI GAG'CTC
Cuts at: 2560 2560
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2375 2837 2951 3968 4565 5352
Size: 393 605 138 462 114 1017 597 787
Cuts at: 5352 5620 1239
Size: 268 1524
Fragments arranged by size:
1524 1017 787 605 597 462 393 268
138 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 2114 2426 3004 2093
Size: 21 312 578 4994
Fragments arranged by size:
4994 578 312 21
EcoO109I rG'GnC_Cy
Cuts at: 2212 2365 2366 2691 3103 2212
Size: 153 1 325 412 5014
Fragments arranged by size:
5014 412 325 153 1
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2089 2139 2289 5052 1678
Size: 411 50 150 2763 2531
Fragments arranged by size:
2763 2531 411 150 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373
Size: 32 135 32 2974 32 42 32 2626
Fragments arranged by size:
2974 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2879 748
Size: 2131 3774
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3053 5019 5203 1305
Size: 1748 1966 184 2007
Fragments arranged by size:
2007 1966 1748 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 10
Size: 54 1425 343 450 3633
Fragments arranged by size:
3633 1425 450 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 2685 2711 3863 4222 4381 518
Size: 648 1284 235 26 1152 359 159 2042
Fragments arranged by size:
2042 1284 1152 648 359 235 159 26
MspA1I CmG'CkG
Cuts at: 1606 2698 3321 3998 5146 5391 1606
Size: 1092 623 677 1148 245 2120
Fragments arranged by size:
2120 1148 1092 677 623 245
NaeI GCC'GGC
Cuts at: 2150 2201 2870 2150
Size: 51 669 5185
Fragments arranged by size:
5185 669 51
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4414
Fragments arranged by size:
4414 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NgoMIV G'CCGG_C
Cuts at: 2148 2199 2868 2148
Size: 51 669 5185
Fragments arranged by size:
5185 669 51
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2495 2575 3263 4808 1132
Size: 97 1266 80 688 1545 2229
Fragments arranged by size:
2229 1545 1266 688 97 80
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 2571 4804 2571
Size: 2233 3672
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 2271 2271
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2691 2691
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2365 2365
Size: 5905
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2288 2320 2288
Size: 32 5873
PstI C_TGCA'G
Cuts at: 830 2190 2901 3257 830
Size: 1360 711 356 3478
Fragments arranged by size:
3478 1360 711 356
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2698 2698
Size: 5905
SacI G_AGCT'C
Cuts at: 2562 2562
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SanDI GG'GwC_CC
Cuts at: 2691 2691
Size: 5905
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SexAI A'CCwGG_T
Cuts at: 2172 2172
Size: 5905
SfcI C'TryA_G
Cuts at: 826 1045 2186 2897 3001 3253 4388 5069 5260
Size: 219 1141 711 104 252 1135 681 191
Cuts at: 5260 826
Size: 1471
Fragments arranged by size:
1471 1141 1135 711 681 252 219 191
104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2004 3148 3344 513
Size: 552 939 1144 196 3074
Fragments arranged by size:
3074 1144 939 552 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2462 2783 3932 3949 4102 4287 4732
Size: 1255 882 321 1149 17 153 185 445
Cuts at: 4732 325
Size: 1498
Fragments arranged by size:
1498 1255 1149 882 445 321 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 4151 5863
Size: 274 80 33 51 1443 597 1577 1712
Cuts at: 5863 96
Size: 138
Fragments arranged by size:
1712 1577 1443 597 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 5865 5897 2222
Size: 32 3611 32 2230
Fragments arranged by size:
3611 2230 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2528 3028 2528
Size: 500 5405
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2956 3713 276
Size: 53 83 186 936 1422 757 2468
Fragments arranged by size:
2468 1422 936 757 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI RsrII
SacII SapI SfiI SfoI SrfI SwaI