Restriction map for
pFN21AE0496
(Circular) (Six-base) MAPSORT of: pFN21AE0496.seq Check: 6212 from: 1 to: 5461
pFN21AE0496 5461 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2095 2095
Size: 5461
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2532 2804 1937
Size: 595 272 4594
Fragments arranged by size:
4594 595 272
Acc65I G'GTAC_C
Cuts at: 2613 2782 2613
Size: 169 5292
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2524 3524 4908 5176 1632
Size: 892 1000 1384 268 1917
Fragments arranged by size:
1917 1384 1000 892 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 2306 4190 2306
Size: 1884 3577
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5461
AlwNI CAG_nnn'CTG
Cuts at: 2060 2526 2689 3204 4776 2060
Size: 466 163 515 1572 2745
Fragments arranged by size:
2745 1572 515 466 163
ApaI G_GGCC'C
Cuts at: 2083 2083
Size: 5461
ApaLI G'TGCA_C
Cuts at: 3518 4674 3518
Size: 1156 4305
ApoI r'AATT_y
Cuts at: 1493 2228 2770 2996 3148 5257 1493
Size: 735 542 226 152 2109 1697
Fragments arranged by size:
2109 1697 735 542 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2233 2720 2786 1870
Size: 73 290 487 66 4545
Fragments arranged by size:
4545 487 290 73 66
BamHI G'GATC_C
Cuts at: 2791 3158 2791
Size: 367 5094
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2613 2715 2782 4237 618
Size: 325 217 627 826 102 67 1455 1842
Fragments arranged by size:
1842 1455 826 627 325 217 102 67
BanII G_rGCy'C
Cuts at: 1391 2083 2754 2780 1391
Size: 692 671 26 4072
Fragments arranged by size:
4072 692 671 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2185 928
Size: 971 286 4204
Fragments arranged by size:
4204 971 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2007 2041 2214 2248 3652 3686 1903
Size: 34 70 34 173 34 1404 34 3678
Fragments arranged by size:
3678 1404 173 70 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2607 3353 4563 2607
Size: 746 1210 3505
Fragments arranged by size:
3505 1210 746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 2156 4072
Size: 107 122 71 631 605 370 114 1916
Cuts at: 4072 136
Size: 1525
Fragments arranged by size:
1916 1525 631 605 370 122 114 107
71
BglII A'GATC_T
Cuts at: 5456 5456
Size: 5461
BlpI GC'TnA_GC
Cuts at: 2878 2878
Size: 5461
Bme1580I G_kGCm'C
Cuts at: 1790 2083 3522 4678 1790
Size: 293 1439 1156 2573
Fragments arranged by size:
2573 1439 1156 293
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2514 3192 4150
Size: 416 53 83 399 24 1097 678 958
Cuts at: 4150 442
Size: 1753
Fragments arranged by size:
1753 1097 958 678 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2554 4121 1239
Size: 1133 182 1567 2579
Fragments arranged by size:
2579 1567 1133 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3586 4451 4749 3586
Size: 865 298 4298
Fragments arranged by size:
4298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2572 4124 882
Size: 617 1073 1552 2219
Fragments arranged by size:
2219 1552 1073 617
BsaAI yAC'GTr
Cuts at: 493 1204 3252 493
Size: 711 2048 2702
Fragments arranged by size:
2702 2048 711
BsaBI GATnn'nnATC
Cuts at: 2345 5455 2345
Size: 3110 2351
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3268 3650 4285 275
Size: 53 83 186 936 1735 382 635 1451
Fragments arranged by size:
1735 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3894 4292 4566 4713 5124 3894
Size: 398 274 147 411 4231
Fragments arranged by size:
4231 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 1178
Size: 30 165 30 1038 30 4168
Fragments arranged by size:
4168 1038 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5461
BseYI C'CCAG_C
Cuts at: 4664 5160 4664
Size: 496 4965
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4888
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2272 2911 3672 4700 1540
Size: 275 186 271 639 761 1028 2301
Fragments arranged by size:
2301 1028 761 639 275 271 186
BsiHKAI G_wGCw'C
Cuts at: 2246 2451 3522 3607 4678 2246
Size: 205 1071 85 1071 3029
Fragments arranged by size:
3029 1071 1071 205 85
BsmI GAATG_Cn'
Cuts at: 2972 3065 2972
Size: 93 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2246 2451 2754 2780 3522 3607
Size: 399 293 163 205 303 26 742 85
Cuts at: 3607 4678 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 293 205 163
85 26
BspEI T'CCGG_A
Cuts at: 5124 5124
Size: 5461
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2095 2816 844
Size: 1251 721 3489
Fragments arranged by size:
3489 1251 721
BsrBI CCG'CTC
Cuts at: 1394 1823 3348 1394
Size: 429 1525 3507
Fragments arranged by size:
3507 1525 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3956 4130 65
Size: 1183 2708 174 1396
Fragments arranged by size:
2708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4105 4292 1700
Size: 110 2295 187 2869
Fragments arranged by size:
2869 2295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5461
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5461
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2689 794
Size: 1895 3566
BstBI TT'CG_AA
Cuts at: 2196 4265 2196
Size: 2069 3392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5461
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2434
Size: 5461
BstYI r'GATC_y
Cuts at: 1972 2499 2791 3158 3543 3560 5012 5098 5110
Size: 527 292 367 385 17 1452 86 12
Cuts at: 5110 5456 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 527 385 367 346 292 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2260 2295 2332 2608 513
Size: 552 1195 35 37 276 3366
Fragments arranged by size:
3366 1195 552 276 37 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4722
Fragments arranged by size:
4722 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 2717 2977 3771 3791 2407
Size: 310 260 794 20 4077
Fragments arranged by size:
4077 794 310 260 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2742 2908
Size: 54 1425 50 275 18 204 708 166
Cuts at: 2908 3797 5426 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 708 275 204 166 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2908 1537
Size: 275 1096 4090
Fragments arranged by size:
4090 1096 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3391 1353
Size: 2038 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 4048 4422 4568 387
Size: 1064 138 585 1874 374 146 1280
Fragments arranged by size:
1874 1280 1064 585 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2372 2524 2554 3524 4121 4908 5176
Size: 393 740 152 30 970 597 787 268
Cuts at: 5176 1239
Size: 1524
Fragments arranged by size:
1524 970 787 740 597 393 268 152
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2354
Size: 5461
EcoO109I rG'GnC_Cy
Cuts at: 2057 2079 2080 2300 2727 2057
Size: 22 1 220 427 4791
Fragments arranged by size:
4791 427 220 22 1
EcoRI G'AATT_C
Cuts at: 2228 2770 3148 2228
Size: 542 378 4541
Fragments arranged by size:
4541 542 378
FspI TGC'GCA
Cuts at: 2279 3967 2279
Size: 1688 3773
HaeII r_GCGC'y
Cuts at: 1678 2151 4608 1678
Size: 473 2457 2531
Fragments arranged by size:
2531 2457 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2292 2324 4102 4134 4176
Size: 32 135 32 720 32 1778 32 42
Cuts at: 4176 4208 1373
Size: 32 2626
Fragments arranged by size:
2626 1778 720 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KpnI G_GTAC'C
Cuts at: 2617 2786 2617
Size: 169 5292
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 4575 4759 1305
Size: 1044 2226 184 2007
Fragments arranged by size:
2226 2007 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2744 10
Size: 54 1425 343 912 2727
Fragments arranged by size:
2727 1425 912 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 2652 3419 3778 3937 518
Size: 648 1266 220 767 359 159 2042
Fragments arranged by size:
2042 1266 767 648 359 220 159
MspA1I CmG'CkG
Cuts at: 1606 2297 2334 2610 2877 3554 4702 4947 1606
Size: 691 37 276 267 677 1148 245 2120
Fragments arranged by size:
2120 1148 691 677 276 267 245 37
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4909
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2819 4364 1132
Size: 97 1590 1545 2229
Fragments arranged by size:
2229 1590 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2054 2304 1348
Size: 706 250 4505
Fragments arranged by size:
4505 706 250
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2526 1108
Size: 1418 4043
PfoI T'CCnGG_A
Cuts at: 2349 2349
Size: 5461
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2300 2727 2300
Size: 427 5034
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PspOMI G'GGCC_C
Cuts at: 2079 2079
Size: 5461
PspXI vC'TCGA_Gb
Cuts at: 2720 2720
Size: 5461
PstI C_TGCA'G
Cuts at: 830 2092 2813 830
Size: 1262 721 3478
Fragments arranged by size:
3478 1262 721
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
SacII CC_GC'GG
Cuts at: 2298 2335 2611 2298
Size: 37 276 5148
Fragments arranged by size:
5148 276 37
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SanDI GG'GwC_CC
Cuts at: 2300 2300
Size: 5461
SbfI CC_TGCA'GG
Cuts at: 2092 2813 2092
Size: 721 4740
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SfcI C'TryA_G
Cuts at: 826 1045 2088 2179 2809 3944 4625 4816 826
Size: 219 1043 91 630 1135 681 191 1471
Fragments arranged by size:
1471 1135 1043 681 630 219 191 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2788 1872
Size: 916 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2720 3565 4466 4728 820
Size: 197 926 777 845 901 262 1553
Fragments arranged by size:
1553 926 901 845 777 262 197
SnaBI TAC'GTA
Cuts at: 493 3252 493
Size: 2759 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2819 1132
Size: 1687 3774
SspI AAT'ATT
Cuts at: 5 52 3385 5
Size: 47 3333 2081
Fragments arranged by size:
3333 2081 47
StuI AGG'CCT
Cuts at: 1517 2024 1517
Size: 507 4954
StyI C'CwwG_G
Cuts at: 513 1065 2067 2151 2757 2900 513
Size: 552 1002 84 606 143 3074
Fragments arranged by size:
3074 1002 606 552 143 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2533 3488 3505 3658 3843 4288 325
Size: 1255 953 955 17 153 185 445 1498
Fragments arranged by size:
1498 1255 955 953 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3707 5419 96
Size: 274 80 33 51 1443 1730 1712 138
Fragments arranged by size:
1730 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2441 2473 5421 5453 2441
Size: 32 2948 32 2449
Fragments arranged by size:
2948 2449 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XhoI C'TCGA_G
Cuts at: 1943 2720 1943
Size: 777 4684
XmaI C'CCGG_G
Cuts at: 1870 2786 1870
Size: 916 4545
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 5461
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PsrI PvuII RsrII SacI
SapI SexAI SfiI SfoI SrfI SwaI XcmI