Restriction map for
pFN21AE0494
(Circular) (Six-base) MAPSORT of: pFN21AE0494.seq Check: 9473 from: 1 to: 5359
pFN21AE0494 5359 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2086 2330 2086
Size: 244 5115
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3169 278
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2702 1937
Size: 765 4594
Acc65I G'GTAC_C
Cuts at: 2680 2680
Size: 5359
AclI AA'CG_TT
Cuts at: 1563 3486 3859 1563
Size: 1923 373 3063
Fragments arranged by size:
3063 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3422 4806 5074 1632
Size: 1790 1384 268 1917
Fragments arranged by size:
1917 1790 1384 268
AfeI AGC'GCT
Cuts at: 2493 2493
Size: 5359
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5162
AflIII A'CryG_T
Cuts at: 1203 4258 5235 1203
Size: 3055 977 1327
Fragments arranged by size:
3055 1327 977
AgeI A'CCGG_T
Cuts at: 4190 4190
Size: 5359
AhdI GACnn_n'nnGTC
Cuts at: 4088 4088
Size: 5359
AleI CACnn'nnGTG
Cuts at: 1166 2231 2324 1166
Size: 1065 93 4201
Fragments arranged by size:
4201 1065 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2249 1184
Size: 1065 4294
AlwNI CAG_nnn'CTG
Cuts at: 3102 4674 3102
Size: 1572 3787
ApaI G_GGCC'C
Cuts at: 2339 2339
Size: 5359
ApaLI G'TGCA_C
Cuts at: 2314 3416 4572 2314
Size: 1102 1156 3101
Fragments arranged by size:
3101 1156 1102
ApoI r'AATT_y
Cuts at: 1493 2668 2894 3046 5155 1493
Size: 1175 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3913 160
Size: 872 2881 1606
Fragments arranged by size:
2881 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
AvaI C'yCGr_G
Cuts at: 1870 1943 2332 2684 1870
Size: 73 389 352 4545
Fragments arranged by size:
4545 389 352 73
BamHI G'GATC_C
Cuts at: 2689 3056 2689
Size: 367 4992
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 2680 4135 618
Size: 325 217 627 768 125 1455 1842
Fragments arranged by size:
1842 1455 768 627 325 217 125
BanII G_rGCy'C
Cuts at: 1391 2339 2678 1391
Size: 948 339 4072
Fragments arranged by size:
4072 948 339
BbeI G_GCGC'C
Cuts at: 2559 2559
Size: 5359
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4388
BbvCI CC'TCA_GC
Cuts at: 2585 2585
Size: 5359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3550 3584 1903
Size: 34 1613 34 3678
Fragments arranged by size:
3678 1613 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3251 4461 3251
Size: 1210 4149
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 2092 2617
Size: 107 122 71 631 605 380 40 525
Cuts at: 2617 3970 136
Size: 1353 1525
Fragments arranged by size:
1525 1353 631 605 525 380 122 107
71 40
BglII A'GATC_T
Cuts at: 5354 5354
Size: 5359
BlpI GC'TnA_GC
Cuts at: 2776 2776
Size: 5359
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2318 2339 2463 3420 4576 1790
Size: 397 131 21 124 957 1156 2573
Fragments arranged by size:
2573 1156 957 397 131 124 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5359
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3090 4048 442
Size: 416 53 83 399 24 1673 958 1753
Fragments arranged by size:
1753 1673 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5359
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2390 2390
Size: 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4019 1239
Size: 2780 2579
Bpu10I CC'TnA_GC
Cuts at: 2585 2585
Size: 5359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3484 4349 4647 3484
Size: 865 298 4196
Fragments arranged by size:
4196 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4022 882
Size: 617 2523 2219
Fragments arranged by size:
2523 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3150 493
Size: 711 1946 2702
Fragments arranged by size:
2702 1946 711
BsaBI GATnn'nnATC
Cuts at: 5353 5353
Size: 5359
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2556 3166 3548 4183
Size: 53 83 186 936 1023 610 382 635
Cuts at: 4183 275
Size: 1451
Fragments arranged by size:
1451 1023 936 635 610 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3792 4190 4464 4611 5022 3792
Size: 398 274 147 411 4129
Fragments arranged by size:
4129 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5134
Fragments arranged by size:
5134 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5359
BseYI C'CCAG_C
Cuts at: 2055 2482 2505 4562 5058 2055
Size: 427 23 2057 496 2356
Fragments arranged by size:
2356 2057 496 427 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 2255 2551 1163
Size: 573 456 63 296 3971
Fragments arranged by size:
3971 573 456 296 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2575 2809 3570 4598 1540
Size: 275 186 574 234 761 1028 2301
Fragments arranged by size:
2301 1028 761 574 275 234 186
BsiHKAI G_wGCw'C
Cuts at: 2180 2228 2318 2376 3420 3505 4576 2180
Size: 48 90 58 1044 85 1071 2963
Fragments arranged by size:
2963 1071 1044 90 85 58 48
BsmI GAATG_Cn'
Cuts at: 2870 2963 2870
Size: 93 5266
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2187 2228 2318 2339 2376 2463
Size: 399 390 7 41 90 21 37 87
Cuts at: 2463 2678 3420 3505 4576 1391
Size: 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 215 90 87
85 41 37 21 7
BspEI T'CCGG_A
Cuts at: 5022 5022
Size: 5359
BspHI T'CATG_A
Cuts at: 3248 4978 3248
Size: 1730 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2086 2330 2714 844
Size: 1242 244 384 3489
Fragments arranged by size:
3489 1242 384 244
BsrBI CCG'CTC
Cuts at: 1394 1823 3246 1394
Size: 429 1423 3507
Fragments arranged by size:
3507 1423 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 4028 65
Size: 1183 2606 174 1396
Fragments arranged by size:
2606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2570 2630 4003 4190 1700
Size: 110 760 60 1373 187 2869
Fragments arranged by size:
2869 1373 760 187 110 60
BsrGI T'GTAC_A
Cuts at: 96 2496 96
Size: 2400 2959
BssHII G'CGCG_C
Cuts at: 1925 2307 1925
Size: 382 4977
BssSI C'ACGA_G
Cuts at: 2402 3419 4431 2402
Size: 1017 1012 3330
Fragments arranged by size:
3330 1017 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2322 794
Size: 1528 3831
BstBI TT'CG_AA
Cuts at: 4163 4163
Size: 5359
BstEII G'GTnAC_C
Cuts at: 1184 2098 1184
Size: 914 4445
BstXI CCAn_nnnn'nTGG
Cuts at: 2326 2415 2326
Size: 89 5270
BstYI r'GATC_y
Cuts at: 1972 2025 2689 3056 3441 3458 4910 4996 5008
Size: 53 664 367 385 17 1452 86 12
Cuts at: 5008 5354 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 664 385 367 346 86 53
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2612 2622 513
Size: 552 939 608 10 3250
Fragments arranged by size:
3250 939 608 552 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4620
Fragments arranged by size:
4620 683 56
BtsI GCAGTG_nn'
Cuts at: 2198 2318 2875 3669 3689 2198
Size: 120 557 794 20 3868
Fragments arranged by size:
3868 794 557 120 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5324
DraI TTT'AAA
Cuts at: 2663 2663
Size: 5359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4366 809
Size: 507 3050 1802
Fragments arranged by size:
3050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 2511 2572
Size: 54 1425 50 275 18 510 171 61
Cuts at: 2572 2625 2806 3695 5324 8
Size: 53 181 889 1629 43
Fragments arranged by size:
1629 1425 889 510 275 181 171 61
54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2572 2806 1537
Size: 275 760 234 4090
Fragments arranged by size:
4090 760 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 3289 1353
Size: 941 995 3423
Fragments arranged by size:
3423 995 941
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3946 4320 4466 387
Size: 1064 138 2357 374 146 1280
Fragments arranged by size:
2357 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3422 4019 4806 5074 1239
Size: 393 1790 597 787 268 1524
Fragments arranged by size:
1790 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2668 3046 2668
Size: 378 4981
FspI TGC'GCA
Cuts at: 3865 3865
Size: 5359
HaeII r_GCGC'y
Cuts at: 1678 2252 2265 2402 2495 2559 2612 4506 1678
Size: 574 13 137 93 64 53 1894 2531
Fragments arranged by size:
2531 1894 574 137 93 64 53 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4000 4032 4074 4106 1373
Size: 32 135 32 2428 32 42 32 2626
Fragments arranged by size:
2626 2428 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 2949 669
Size: 988 281 765 246 3079
Fragments arranged by size:
3079 988 765 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5359
HpaI GTT'AAC
Cuts at: 2949 2949
Size: 5359
KasI G'GCGC_C
Cuts at: 2555 2555
Size: 5359
KpnI G_GTAC'C
Cuts at: 2684 2684
Size: 5359
MfeI C'AATT_G
Cuts at: 2958 2958
Size: 5359
MluI A'CGCG_T
Cuts at: 5235 5235
Size: 5359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 4473 4657 1305
Size: 696 2472 184 2007
Fragments arranged by size:
2472 2007 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3537
Fragments arranged by size:
3537 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2324 3317 3676 3835 518
Size: 648 1065 93 993 359 159 2042
Fragments arranged by size:
2042 1065 993 648 359 159 93
MspA1I CmG'CkG
Cuts at: 1606 2614 2775 3452 4600 4845 1606
Size: 1008 161 677 1148 245 2120
Fragments arranged by size:
2120 1148 1008 677 245 161
NarI GG'CG_CC
Cuts at: 2556 2556
Size: 5359
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3868
Fragments arranged by size:
3868 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5359
NotI GC'GGCC_GC
Cuts at: 2806 2806
Size: 5359
NspI r_CATG'y
Cuts at: 1132 1229 2191 2717 4262 1132
Size: 97 962 526 1545 2229
Fragments arranged by size:
2229 1545 962 526 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5359
PciI A'CATG_T
Cuts at: 4258 4258
Size: 5359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5359
PfoI T'CCnGG_A
Cuts at: 2109 2109
Size: 5359
PmeI GTTT'AAAC
Cuts at: 2663 2663
Size: 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 4941 4973 3428
Size: 32 1481 32 3814
Fragments arranged by size:
3814 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5359
PsiI TTA'TAA
Cuts at: 2929 2929
Size: 5359
PspOMI G'GGCC_C
Cuts at: 2335 2335
Size: 5359
PstI C_TGCA'G
Cuts at: 830 2711 830
Size: 1881 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5359
SacII CC_GC'GG
Cuts at: 2615 2615
Size: 5359
SalI G'TCGA_C
Cuts at: 1936 2701 1936
Size: 765 4594
SapI GCTCTTCn'nnn_
Cuts at: 2294 2294
Size: 5359
SbfI CC_TGCA'GG
Cuts at: 2711 2711
Size: 5359
ScaI AGT'ACT
Cuts at: 2603 3607 5319 2603
Size: 1004 1712 2643
Fragments arranged by size:
2643 1712 1004
SfcI C'TryA_G
Cuts at: 826 1045 2707 3842 4523 4714 826
Size: 219 1662 1135 681 191 1471
Fragments arranged by size:
1662 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2557 2557
Size: 5359
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
SgrAI Cr'CCGG_yG
Cuts at: 1700 2630 1700
Size: 930 4429
SmaI CCC'GGG
Cuts at: 1872 2334 2686 1872
Size: 462 352 4545
Fragments arranged by size:
4545 462 352
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3463 4364 4626 820
Size: 197 926 1520 901 262 1553
Fragments arranged by size:
1553 1520 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3150 493
Size: 2657 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5359
SphI G_CATG'C
Cuts at: 1132 2717 1132
Size: 1585 3774
SspI AAT'ATT
Cuts at: 5 52 3283 5
Size: 47 3231 2081
Fragments arranged by size:
3231 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5359
StyI C'CwwG_G
Cuts at: 513 1065 2004 2343 2798 513
Size: 552 939 339 455 3074
Fragments arranged by size:
3074 939 552 455 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 3403 3556 3741 4186 325
Size: 1255 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 2601 3605
Size: 274 80 33 51 1443 519 105 1004
Cuts at: 3605 5317 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1004 519 274 138 105 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2560 2592 5319 5351 2560
Size: 32 2727 32 2568
Fragments arranged by size:
2727 2568 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5359
XbaI T'CTAG_A
Cuts at: 2695 2695
Size: 5359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5359
XmaI C'CCGG_G
Cuts at: 1870 2332 2684 1870
Size: 462 352 4545
Fragments arranged by size:
4545 462 352
XmnI GAAnn'nnTTC
Cuts at: 3488 3488
Size: 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3167 276
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI
SrfI SwaI