Restriction map for
pFN21AE0490
(Circular) (Six-base) MAPSORT of: pFN21AE0490.seq Check: 6174 from: 1 to: 5926
pFN21AE0490 5926 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2217 2483 3088 2217
Size: 266 605 5055
Fragments arranged by size:
5055 605 266
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2625 3736 278
Size: 53 83 186 936 1089 1111 2468
Fragments arranged by size:
2468 1111 1089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3989 5373 5641 1632
Size: 2357 1384 268 1917
Fragments arranged by size:
2357 1917 1384 268
AfeI AGC'GCT
Cuts at: 2240 2240
Size: 5926
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 2328 4825 5802 1203
Size: 1125 2497 977 1327
Fragments arranged by size:
2497 1327 1125 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 3669 5241 3669
Size: 1572 4354
ApaI G_GGCC'C
Cuts at: 3126 3126
Size: 5926
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 3221 3235 3461 3613 5722 1493
Size: 1728 14 226 152 2109 1697
Fragments arranged by size:
2109 1728 1697 226 152 14
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2712 3251 1870
Size: 73 144 625 539 4545
Fragments arranged by size:
4545 625 539 144 73
AvrII C'CTAG_G
Cuts at: 2404 2404
Size: 5926
BamHI G'GATC_C
Cuts at: 2035 3256 3623 2035
Size: 1221 367 4338
Fragments arranged by size:
4338 1221 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2339 2471 2867 3140 3176
Size: 325 217 627 552 132 396 273 36
Cuts at: 3176 3247 4702 618
Size: 71 1455 1842
Fragments arranged by size:
1842 1455 627 552 396 325 273 217
132 71 36
BanII G_rGCy'C
Cuts at: 1391 3126 3245 1391
Size: 1735 119 4072
Fragments arranged by size:
4072 1735 119
BbeI G_GCGC'C
Cuts at: 3144 3180 3144
Size: 36 5890
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3200 928
Size: 971 1301 3654
Fragments arranged by size:
3654 1301 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2046 2080 4117 4151 1903
Size: 34 109 34 2037 34 3678
Fragments arranged by size:
3678 2037 109 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2144 3130 3174
Size: 107 122 71 631 605 472 986 44
Cuts at: 3174 4537 136
Size: 1363 1525
Fragments arranged by size:
1525 1363 986 631 605 472 122 107
71 44
BglII A'GATC_T
Cuts at: 2196 5921 2196
Size: 3725 2201
BlpI GC'TnA_GC
Cuts at: 2294 2723 3206 3343 2294
Size: 429 483 137 4877
Fragments arranged by size:
4877 483 429 137
Bme1580I G_kGCm'C
Cuts at: 1790 2271 2870 2998 3126 3987 5143 1790
Size: 481 599 128 128 861 1156 2573
Fragments arranged by size:
2573 1156 861 599 481 128 128
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3657 4615 442
Size: 416 53 83 399 24 2240 958 1753
Fragments arranged by size:
2240 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 2831 2998 3134 4586 1239
Size: 1331 261 167 136 1452 2579
Fragments arranged by size:
2579 1452 1331 261 167 136
Bpu10I CC'TnA_GC
Cuts at: 2142 2384 2142
Size: 242 5684
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 3008 4051 4916 5214 2729
Size: 279 1043 865 298 3441
Fragments arranged by size:
3441 1043 865 298 279
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3023 4589 882
Size: 617 1524 1566 2219
Fragments arranged by size:
2219 1566 1524 617
BsaAI yAC'GTr
Cuts at: 493 1204 2329 2644 3717 493
Size: 711 1125 315 1073 2702
Fragments arranged by size:
2702 1125 1073 711 315
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2622 3060 3141 3177
Size: 53 83 186 936 1089 438 81 36
Cuts at: 3177 3733 4115 4750 275
Size: 556 382 635 1451
Fragments arranged by size:
1451 1089 936 635 556 438 382 186
83 81 53 36
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2648 2678 3137 3167 1178
Size: 30 165 30 1245 30 459 30 3937
Fragments arranged by size:
3937 1245 459 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2424 1186
Size: 1105 133 4688
Fragments arranged by size:
4688 1105 133
BseYI C'CCAG_C
Cuts at: 2069 5129 5625 2069
Size: 3060 496 2370
Fragments arranged by size:
3060 2370 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3074 1163
Size: 573 1338 4015
Fragments arranged by size:
4015 1338 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 3376 4137 5165 1540
Size: 275 186 54 1321 761 1028 2301
Fragments arranged by size:
2301 1321 1028 761 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 3987 4072 5143 3987
Size: 85 1071 4770
Fragments arranged by size:
4770 1071 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
BsmBI CGTCTCn'nnnn_
Cuts at: 2080 2080
Size: 5926
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2271 2870 2998 3126 3245 3987 4072
Size: 399 481 599 128 128 119 742 85
Cuts at: 4072 5143 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 599 481 399 128 128
119 85
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2483 3088 3281 844
Size: 1373 266 605 193 3489
Fragments arranged by size:
3489 1373 605 266 193
BsrBI CCG'CTC
Cuts at: 1394 1823 2141 3159 3813 1394
Size: 429 318 1018 654 3507
Fragments arranged by size:
3507 1018 654 429 318
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2879 4570 4757 1700
Size: 110 1069 1691 187 2869
Fragments arranged by size:
2869 1691 1069 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2478 2794 794
Size: 1684 316 3926
Fragments arranged by size:
3926 1684 316
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2107
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2035 2196 3256 3623 4008 4025 5477 5563
Size: 63 161 1060 367 385 17 1452 86
Cuts at: 5563 5575 5921 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1060 385 367 346 161 86
63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2106 2871 3145 513
Size: 552 1041 765 274 3294
Fragments arranged by size:
3294 1041 765 552 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 3442 4236 4256 2100
Size: 1342 794 20 3770
Fragments arranged by size:
3770 1342 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2621 4933 809
Size: 507 1305 2312 1802
Fragments arranged by size:
2312 1802 1305 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2802 3373 4262
Size: 54 1425 50 275 18 972 571 889
Cuts at: 4262 5891 8
Size: 1629 43
Fragments arranged by size:
1629 1425 972 889 571 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 2309 2696 2702 2999 3856 1353
Size: 911 45 387 6 297 857 3423
Fragments arranged by size:
3423 911 857 387 297 45 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2570 2831 2998 3134 3989 4586 5373
Size: 393 938 261 167 136 855 597 787
Cuts at: 5373 5641 1239
Size: 268 1524
Fragments arranged by size:
1524 938 855 787 597 393 268 261
167 136
EcoRI G'AATT_C
Cuts at: 3221 3235 3613 3221
Size: 14 378 5534
Fragments arranged by size:
5534 378 14
FspI TGC'GCA
Cuts at: 2252 4432 2252
Size: 2180 3746
FspAI rTGC'GCAy
Cuts at: 2252 2252
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2242 3144 3180 5073 1678
Size: 564 902 36 1893 2531
Fragments arranged by size:
2531 1893 902 564 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 4567 4599 4641
Size: 32 135 32 455 32 2508 32 42
Cuts at: 4641 4673 1373
Size: 32 2626
Fragments arranged by size:
2626 2508 455 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 3140 3176 3140
Size: 36 5890
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 5040 5224 1305
Size: 1104 2631 184 2007
Fragments arranged by size:
2631 2007 1104 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2804 10
Size: 54 1425 343 972 3132
Fragments arranged by size:
3132 1425 972 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2105 2903 3884 4243 4402 518
Size: 648 843 96 798 981 359 159 2042
Fragments arranged by size:
2042 981 843 798 648 359 159 96
MspA1I CmG'CkG
Cuts at: 1606 2223 2460 2991 3342 4019 5167 5412 1606
Size: 617 237 531 351 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 617 531 351 245 237
NaeI GCC'GGC
Cuts at: 2881 2881
Size: 5926
NarI GG'CG_CC
Cuts at: 3141 3177 3141
Size: 36 5890
NcoI C'CATG_G
Cuts at: 513 1065 2106 513
Size: 552 1041 4333
Fragments arranged by size:
4333 1041 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NgoMIV G'CCGG_C
Cuts at: 2879 2879
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2190 2206 2797 3284 4829 1132
Size: 97 961 16 591 487 1545 2229
Fragments arranged by size:
2229 1545 961 591 487 97 16
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5926
PfoI T'CCnGG_A
Cuts at: 2808 2808
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PspOMI G'GGCC_C
Cuts at: 3122 3122
Size: 5926
PstI C_TGCA'G
Cuts at: 830 2541 3278 830
Size: 1711 737 3478
Fragments arranged by size:
3478 1711 737
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2223 2460 2223
Size: 237 5689
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SapI GCTCTTCn'nnn_
Cuts at: 2999 2999
Size: 5926
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 2324 4174 5886 2324
Size: 1850 1712 2364
Fragments arranged by size:
2364 1850 1712
SfcI C'TryA_G
Cuts at: 826 1045 2218 2537 2731 3274 4409 5090 5281
Size: 219 1173 319 194 543 1135 681 191
Cuts at: 5281 826
Size: 1471
Fragments arranged by size:
1471 1173 1135 681 543 319 219 194
191
SfoI GGC'GCC
Cuts at: 3142 3178 3142
Size: 36 5890
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 2714 3253 1872
Size: 842 539 4545
Fragments arranged by size:
4545 842 539
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 3023 4030 4931 5193 820
Size: 197 926 801 279 1007 901 262 1553
Fragments arranged by size:
1553 1007 926 901 801 279 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 2797 3284 1132
Size: 1665 487 3774
Fragments arranged by size:
3774 1665 487
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 2363 2591 1517
Size: 846 228 4852
Fragments arranged by size:
4852 846 228
StyI C'CwwG_G
Cuts at: 513 1065 2106 2364 2404 3365 513
Size: 552 1041 258 40 961 3074
Fragments arranged by size:
3074 1041 961 552 258 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3953 3970 4123 4308 4753 325
Size: 1255 2373 17 153 185 445 1498
Fragments arranged by size:
2373 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2331 3093
Size: 274 80 33 51 1443 345 9 762
Cuts at: 3093 4172 5884 96
Size: 1079 1712 138
Fragments arranged by size:
1712 1443 1079 762 345 274 138 80
51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3137 3169 5886 5918 3137
Size: 32 2717 32 3145
Fragments arranged by size:
3145 2717 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3170 3170
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 2712 3251 1870
Size: 842 539 4545
Fragments arranged by size:
4545 842 539
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2623 3734 276
Size: 53 83 186 936 1089 1111 2468
Fragments arranged by size:
2468 1111 1089 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI