Restriction map for
pFN21AE0487
(Circular) (Six-base) MAPSORT of: pFN21AE0487.seq Check: 7591 from: 1 to: 5776
pFN21AE0487 5776 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2226 2634 3586 278
Size: 53 83 186 936 690 408 952 2468
Fragments arranged by size:
2468 952 936 690 408 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2816 3839 5223 5491 1632
Size: 1184 1023 1384 268 1917
Fragments arranged by size:
1917 1384 1184 1023 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2925 1184
Size: 1741 4035
AlwNI CAG_nnn'CTG
Cuts at: 2092 2945 3056 3519 5091 2092
Size: 853 111 463 1572 2777
Fragments arranged by size:
2777 1572 853 463 111
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 3101 1870
Size: 73 1158 4545
Fragments arranged by size:
4545 1158 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2426 3097 4552 618
Size: 325 217 627 639 671 1455 1842
Fragments arranged by size:
1842 1455 671 639 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3095 1391
Size: 1704 4072
BbeI G_GCGC'C
Cuts at: 2430 2430
Size: 5776
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2814 928
Size: 971 915 3890
Fragments arranged by size:
3890 971 915
BbvCI CC'TCA_GC
Cuts at: 2408 2408
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2872 3668 4878 2872
Size: 796 1210 3770
Fragments arranged by size:
3770 1210 796
BclI T'GATC_A
Cuts at: 1548 2722 1548
Size: 1174 4602
BfrBI ATG'CAT
Cuts at: 2981 2981
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4387 136
Size: 107 122 71 631 605 2715 1525
Fragments arranged by size:
2715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2919 5771 2919
Size: 2852 2924
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2550 3837 4993 1790
Size: 760 1287 1156 2573
Fragments arranged by size:
2573 1287 1156 760
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2387 2483 2648 3087 4436 1239
Size: 983 165 96 165 439 1349 2579
Fragments arranged by size:
2579 1349 983 439 165 165 96
Bpu10I CC'TnA_GC
Cuts at: 2408 2408
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2622 3901 4766 5064 2622
Size: 1279 865 298 3334
Fragments arranged by size:
3334 1279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2705 4439 882
Size: 617 1206 1734 2219
Fragments arranged by size:
2219 1734 1206 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 2946 5770 2946
Size: 2824 2952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 2223 2427 2562
Size: 53 83 186 936 582 108 204 135
Cuts at: 2562 2631 3583 3965 4600 275
Size: 69 952 382 635 1451
Fragments arranged by size:
1451 952 936 635 582 382 204 186
135 108 83 69 53
BsaWI w'CCGG_w
Cuts at: 2073 2235 2289 2379 4209 4607 4881 5028 5439
Size: 162 54 90 1830 398 274 147 411
Cuts at: 5439 2073
Size: 2410
Fragments arranged by size:
2410 1830 411 398 274 162 147 90
54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3060 3090 1178
Size: 30 165 30 1657 30 3864
Fragments arranged by size:
3864 1657 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5776
BseYI C'CCAG_C
Cuts at: 2324 4979 5475 2324
Size: 2655 496 2625
Fragments arranged by size:
2655 2625 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 2828 1163
Size: 573 459 633 4111
Fragments arranged by size:
4111 633 573 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2781 3837 3922 4993 2781
Size: 1056 85 1071 3564
Fragments arranged by size:
3564 1071 1056 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
BsmBI CGTCTCn'nnnn_
Cuts at: 2214 2214
Size: 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2550 2781 3095 3837 3922 4993 1391
Size: 399 760 231 314 742 85 1071 2174
Fragments arranged by size:
2174 1071 760 742 399 314 231 85
BspEI T'CCGG_A
Cuts at: 2073 2235 2289 2379 5439 2073
Size: 162 54 90 3060 2410
Fragments arranged by size:
3060 2410 162 90 54
BspHI T'CATG_A
Cuts at: 2719 3665 5395 2719
Size: 946 1730 3100
Fragments arranged by size:
3100 1730 946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2466 3131 844
Size: 1622 665 3489
Fragments arranged by size:
3489 1622 665
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2475 4420 4607 1700
Size: 110 665 1945 187 2869
Fragments arranged by size:
2869 1945 665 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 2155 3836 4848 2155
Size: 1681 1012 3083
Fragments arranged by size:
3083 1681 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2785 794
Size: 1991 3785
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 2971 2971
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2919 3106 3473 3858 3875 5327 5413 5425
Size: 947 187 367 385 17 1452 86 12
Cuts at: 5425 5771 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 947 385 367 346 187 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2430 2493 513
Size: 552 939 426 63 3796
Fragments arranged by size:
3796 939 552 426 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2328 487
Size: 683 56 1102 3935
Fragments arranged by size:
3935 1102 683 56
BtsI GCAGTG_nn'
Cuts at: 2133 3292 4086 4106 2133
Size: 1159 794 20 3803
Fragments arranged by size:
3803 1159 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 2061 3080 2061
Size: 1019 4757
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2648 4783 809
Size: 507 1332 2135 1802
Fragments arranged by size:
2135 1802 1332 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2473 3223 4112
Size: 54 1425 50 275 18 643 750 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 750 643 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3006 3706 1353
Size: 1653 700 3423
Fragments arranged by size:
3423 1653 700
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2387 2483 2648 2816 3087 3839
Size: 393 590 165 96 165 168 271 752
Cuts at: 3839 4436 5223 5491 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 752 597 590 393 271 268
168 165 165 96
EcoNI CCTnn'n_nnAGG
Cuts at: 2307 2348 2462 2307
Size: 41 114 5621
Fragments arranged by size:
5621 114 41
EcoO109I rG'GnC_Cy
Cuts at: 2260 2527 2737 2260
Size: 267 210 5299
Fragments arranged by size:
5299 267 210
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2430 4923 1678
Size: 752 2493 2531
Fragments arranged by size:
2531 2493 752
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2287 2319 4417 4449 4491
Size: 32 135 32 715 32 2098 32 42
Cuts at: 4491 4523 1373
Size: 32 2626
Fragments arranged by size:
2626 2098 715 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KasI G'GCGC_C
Cuts at: 2426 2426
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 3734 4093 4252 518
Size: 648 1623 945 359 159 2042
Fragments arranged by size:
2042 1623 945 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2200 3192 3869 5017 5262 1606
Size: 594 992 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 594 245
NaeI GCC'GGC
Cuts at: 2477 2477
Size: 5776
NarI GG'CG_CC
Cuts at: 2427 2427
Size: 5776
NcoI C'CATG_G
Cuts at: 513 1065 2004 2430 2493 513
Size: 552 939 426 63 3796
Fragments arranged by size:
3796 939 552 426 63
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NgoMIV G'CCGG_C
Cuts at: 2475 2475
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NsiI A_TGCA'T
Cuts at: 2983 2983
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2788 2985 3134 4679 1132
Size: 97 1559 197 149 1545 2229
Fragments arranged by size:
2229 1559 1545 197 149 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PfoI T'CCnGG_A
Cuts at: 2334 2890 2334
Size: 556 5220
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 2527 2260
Size: 267 5509
PshAI GACnn'nnGTC
Cuts at: 1613 2114 2525 1613
Size: 501 411 4864
Fragments arranged by size:
4864 501 411
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2194 2400 3128 830
Size: 1364 206 728 3478
Fragments arranged by size:
3478 1364 728 206
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2200 2200
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2190 2396 3124 4259 4940 5131 826
Size: 219 1145 206 728 1135 681 191 1471
Fragments arranged by size:
1471 1145 1135 728 681 219 206 191
SfoI GGC'GCC
Cuts at: 2428 2428
Size: 5776
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2637 3880 4781 5043 820
Size: 197 926 694 1243 901 262 1553
Fragments arranged by size:
1553 1243 926 901 694 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 2985 3134 1132
Size: 1853 149 3774
Fragments arranged by size:
3774 1853 149
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 2468 1517
Size: 951 4825
StyI C'CwwG_G
Cuts at: 513 1065 2004 2430 2493 2523 3215 513
Size: 552 939 426 63 30 692 3074
Fragments arranged by size:
3074 939 692 552 426 63 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3803 3820 3973 4158 4603 325
Size: 1255 2223 17 153 185 445 1498
Fragments arranged by size:
2223 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2500 2011
Size: 489 5287
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2224 2632 3584 276
Size: 53 83 186 936 690 408 952 2468
Fragments arranged by size:
2468 952 936 690 408 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI