Restriction map for
pFN21AE0485
(Circular) (Six-base) MAPSORT of: pFN21AE0485.seq Check: 8962 from: 1 to: 5302
pFN21AE0485 5302 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3112 278
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2645 1937
Size: 708 4594
Acc65I G'GTAC_C
Cuts at: 2623 2623
Size: 5302
AclI AA'CG_TT
Cuts at: 1563 2601 3429 3802 1563
Size: 1038 828 373 3063
Fragments arranged by size:
3063 1038 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2330 3365 4749 5017 1632
Size: 698 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1035 698 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5105
AflIII A'CryG_T
Cuts at: 1203 2359 4201 5178 1203
Size: 1156 1842 977 1327
Fragments arranged by size:
1842 1327 1156 977
AgeI A'CCGG_T
Cuts at: 4133 4133
Size: 5302
AhdI GACnn_n'nnGTC
Cuts at: 4031 4031
Size: 5302
AleI CACnn'nnGTG
Cuts at: 1166 2360 2498 1166
Size: 1194 138 3970
Fragments arranged by size:
3970 1194 138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5302
AlwNI CAG_nnn'CTG
Cuts at: 3045 4617 3045
Size: 1572 3730
ApaI G_GGCC'C
Cuts at: 2031 2449 2031
Size: 418 4884
ApaLI G'TGCA_C
Cuts at: 3359 4515 3359
Size: 1156 4146
ApoI r'AATT_y
Cuts at: 1493 2474 2611 2837 2989 5098 1493
Size: 981 137 226 152 2109 1697
Fragments arranged by size:
2109 1697 981 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 3856 160
Size: 872 2824 1606
Fragments arranged by size:
2824 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 1870
Size: 73 684 4545
Fragments arranged by size:
4545 684 73
BamHI G'GATC_C
Cuts at: 2044 2632 2999 2044
Size: 588 367 4347
Fragments arranged by size:
4347 588 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2623 4078 618
Size: 325 217 627 345 491 1455 1842
Fragments arranged by size:
1842 1455 627 491 345 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 2303 2449 2621 1391
Size: 640 272 146 172 4072
Fragments arranged by size:
4072 640 272 172 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3493 3527 1903
Size: 34 1556 34 3678
Fragments arranged by size:
3678 1556 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3194 4404 3194
Size: 1210 4092
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3913 136
Size: 107 122 71 631 605 2241 1525
Fragments arranged by size:
2241 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2268 5297 2268
Size: 3029 2273
BlpI GC'TnA_GC
Cuts at: 2719 2719
Size: 5302
Bme1580I G_kGCm'C
Cuts at: 1790 2031 2449 3363 4519 1790
Size: 241 418 914 1156 2573
Fragments arranged by size:
2573 1156 914 418 241
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5302
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3033 3991 442
Size: 416 53 83 399 24 1616 958 1753
Fragments arranged by size:
1753 1616 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2317 2570 3962 1239
Size: 1078 253 1392 2579
Fragments arranged by size:
2579 1392 1078 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3427 4292 4590 3427
Size: 865 298 4139
Fragments arranged by size:
4139 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3965 882
Size: 617 2466 2219
Fragments arranged by size:
2466 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2362 3093 493
Size: 711 1158 731 2702
Fragments arranged by size:
2702 1158 731 711
BsaBI GATnn'nnATC
Cuts at: 5296 5296
Size: 5302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3109 3491 4126 275
Size: 53 83 186 936 1576 382 635 1451
Fragments arranged by size:
1576 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3735 4133 4407 4554 4965 3735
Size: 398 274 147 411 4072
Fragments arranged by size:
4072 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5077
Fragments arranged by size:
5077 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5302
BseYI C'CCAG_C
Cuts at: 2017 2441 2487 4505 5001 2017
Size: 424 46 2018 496 2318
Fragments arranged by size:
2318 2018 496 424 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4729
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2209 2752 3513 4541 1540
Size: 275 186 208 543 761 1028 2301
Fragments arranged by size:
2301 1028 761 543 275 208 186
BsiHKAI G_wGCw'C
Cuts at: 2550 3363 3448 4519 2550
Size: 813 85 1071 3333
Fragments arranged by size:
3333 1071 813 85
BsmI GAATG_Cn'
Cuts at: 2813 2906 2813
Size: 93 5209
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2303 2449 2550 2621 3363 3448
Size: 399 241 272 146 101 71 742 85
Cuts at: 3448 4519 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 272 241 146 101
85 71
BspEI T'CCGG_A
Cuts at: 4965 4965
Size: 5302
BspHI T'CATG_A
Cuts at: 2460 3191 4921 2460
Size: 731 1730 2841
Fragments arranged by size:
2841 1730 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2657 844
Size: 1813 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3189 1394
Size: 429 1366 3507
Fragments arranged by size:
3507 1366 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3797 3971 65
Size: 1183 2549 174 1396
Fragments arranged by size:
2549 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3946 4133 1700
Size: 110 2136 187 2869
Fragments arranged by size:
2869 2136 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5302
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5302
BssSI C'ACGA_G
Cuts at: 3362 4374 3362
Size: 1012 4290
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2431 794
Size: 1637 3665
BstBI TT'CG_AA
Cuts at: 4106 4106
Size: 5302
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5302
BstYI r'GATC_y
Cuts at: 1972 2044 2268 2632 2999 3384 3401 4853 4939
Size: 72 224 364 367 385 17 1452 86
Cuts at: 4939 4951 5297 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 364 346 224 86
72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3811
Fragments arranged by size:
3811 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4563
Fragments arranged by size:
4563 683 56
BtsI GCAGTG_nn'
Cuts at: 2420 2482 2818 3612 3632 2420
Size: 62 336 794 20 4090
Fragments arranged by size:
4090 794 336 62 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5267
DraI TTT'AAA
Cuts at: 2606 2606
Size: 5302
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4309 809
Size: 507 2993 1802
Fragments arranged by size:
2993 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2749 3638
Size: 54 1425 50 275 18 376 543 889
Cuts at: 3638 5267 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 543 376 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2206 2749 1537
Size: 275 394 543 4090
Fragments arranged by size:
4090 543 394 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3232 1353
Size: 1879 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3889 4263 4409 387
Size: 1064 138 2300 374 146 1280
Fragments arranged by size:
2300 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2330 2570 3365 3962 4749 5017
Size: 393 685 13 240 795 597 787 268
Cuts at: 5017 1239
Size: 1524
Fragments arranged by size:
1524 795 787 685 597 393 268 240
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 2411
Size: 5302
EcoO109I rG'GnC_Cy
Cuts at: 2445 2445
Size: 5302
EcoRI G'AATT_C
Cuts at: 2611 2989 2611
Size: 378 4924
FspI TGC'GCA
Cuts at: 3808 3808
Size: 5302
HaeII r_GCGC'y
Cuts at: 1678 2122 4449 1678
Size: 444 2327 2531
Fragments arranged by size:
2531 2327 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2358 2390 3943 3975 4017
Size: 32 135 32 786 32 1553 32 42
Cuts at: 4017 4049 1373
Size: 32 2626
Fragments arranged by size:
2626 1553 786 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 2892 669
Size: 988 281 708 246 3079
Fragments arranged by size:
3079 988 708 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5302
HpaI GTT'AAC
Cuts at: 2892 2892
Size: 5302
KpnI G_GTAC'C
Cuts at: 2627 2627
Size: 5302
MfeI C'AATT_G
Cuts at: 2901 2901
Size: 5302
MluI A'CGCG_T
Cuts at: 5178 5178
Size: 5302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2088 2235 4416 4600 1305
Size: 783 147 2181 184 2007
Fragments arranged by size:
2181 2007 783 184 147
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3480
Fragments arranged by size:
3480 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2498 3260 3619 3778 518
Size: 648 1194 138 762 359 159 2042
Fragments arranged by size:
2042 1194 762 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2718 3395 4543 4788 1606
Size: 1112 677 1148 245 2120
Fragments arranged by size:
2120 1148 1112 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3811
Fragments arranged by size:
3811 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5302
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5302
NotI GC'GGCC_GC
Cuts at: 2749 2749
Size: 5302
NspI r_CATG'y
Cuts at: 1132 1229 2660 4205 1132
Size: 97 1431 1545 2229
Fragments arranged by size:
2229 1545 1431 97
PasI CC'CwG_GG
Cuts at: 1348 2529 1348
Size: 1181 4121
PciI A'CATG_T
Cuts at: 4201 4201
Size: 5302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5302
PfoI T'CCnGG_A
Cuts at: 2294 2294
Size: 5302
PmeI GTTT'AAAC
Cuts at: 2606 2606
Size: 5302
PmlI CAC'GTG
Cuts at: 2362 2362
Size: 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 4884 4916 3371
Size: 32 1481 32 3757
Fragments arranged by size:
3757 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5302
PsiI TTA'TAA
Cuts at: 2872 2872
Size: 5302
PspOMI G'GGCC_C
Cuts at: 2027 2445 2027
Size: 418 4884
PstI C_TGCA'G
Cuts at: 830 2654 830
Size: 1824 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5302
SalI G'TCGA_C
Cuts at: 1936 2644 1936
Size: 708 4594
SbfI CC_TGCA'GG
Cuts at: 2654 2654
Size: 5302
ScaI AGT'ACT
Cuts at: 3550 5262 3550
Size: 1712 3590
SexAI A'CCwGG_T
Cuts at: 2235 2235
Size: 5302
SfcI C'TryA_G
Cuts at: 826 1045 2272 2650 3785 4466 4657 826
Size: 219 1227 378 1135 681 191 1471
Fragments arranged by size:
1471 1227 1135 681 378 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5302
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5302
SmaI CCC'GGG
Cuts at: 1872 2629 1872
Size: 757 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3406 4307 4569 820
Size: 197 926 1463 901 262 1553
Fragments arranged by size:
1553 1463 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3093 493
Size: 2600 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5302
SphI G_CATG'C
Cuts at: 1132 2660 1132
Size: 1528 3774
SspI AAT'ATT
Cuts at: 5 52 3226 5
Size: 47 3174 2081
Fragments arranged by size:
3174 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5302
StyI C'CwwG_G
Cuts at: 513 1065 2004 2741 513
Size: 552 939 737 3074
Fragments arranged by size:
3074 939 737 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2128 3329 3346 3499 3684 4129 325
Size: 1255 548 1201 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1201 548 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3548 5260 96
Size: 274 80 33 51 1443 1571 1712 138
Fragments arranged by size:
1712 1571 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5262 5294 5262
Size: 32 5270
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5302
XbaI T'CTAG_A
Cuts at: 2638 2638
Size: 5302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2531 2561 2038
Size: 493 30 4779
Fragments arranged by size:
4779 493 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5302
XmaI C'CCGG_G
Cuts at: 1870 2627 1870
Size: 757 4545
XmnI GAAnn'nnTTC
Cuts at: 3431 3431
Size: 5302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3110 276
Size: 53 83 186 936 1576 2468
Fragments arranged by size:
2468 1576 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI