Restriction map for
pFN21AE0481
(Circular) (Six-base) MAPSORT of: pFN21AE0481.seq Check: 5249 from: 1 to: 5962
pFN21AE0481 5962 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2256 2633 3305 1937
Size: 319 377 672 4594
Fragments arranged by size:
4594 672 377 319
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4025 5409 5677 1632
Size: 2393 1384 268 1917
Fragments arranged by size:
2393 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 3206 3705 5277 3206
Size: 499 1572 3891
Fragments arranged by size:
3891 1572 499
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3012 3271 3497 3649 5758 1493
Size: 1519 259 226 152 2109 1697
Fragments arranged by size:
2109 1697 1519 259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3108 4516 160
Size: 872 2076 1408 1606
Fragments arranged by size:
2076 1606 1408 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 3287 1870
Size: 73 400 944 4545
Fragments arranged by size:
4545 944 400 73
BamHI G'GATC_C
Cuts at: 2357 2883 3292 3659 2357
Size: 526 409 367 4660
Fragments arranged by size:
4660 526 409 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3283 4738 618
Size: 325 217 627 1496 1455 1842
Fragments arranged by size:
1842 1496 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2058 3177 3281 1391
Size: 667 1119 104 4072
Fragments arranged by size:
4072 1119 667 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4991
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BfrBI ATG'CAT
Cuts at: 2318 2318
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 4023 5179 1790
Size: 2233 1156 2573
Fragments arranged by size:
2573 2233 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2663 2687 2892
Size: 416 53 83 399 24 1246 24 205
Cuts at: 2892 2966 3693 4651 442
Size: 74 727 958 1753
Fragments arranged by size:
1753 1246 958 727 416 399 205 83
74 53 24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3068
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2443 2456 2508 4622 1239
Size: 1204 13 52 2114 2579
Fragments arranged by size:
2579 2114 1204 52 13
Bpu10I CC'TnA_GC
Cuts at: 2445 2445
Size: 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4087 4952 5250 4087
Size: 865 298 4799
Fragments arranged by size:
4799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2477 4625 882
Size: 617 978 2148 2219
Fragments arranged by size:
2219 2148 978 617
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5737
Fragments arranged by size:
5737 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2415 2699 1186
Size: 1229 284 4449
Fragments arranged by size:
4449 1229 284
BseYI C'CCAG_C
Cuts at: 5165 5661 5165
Size: 496 5466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2014 1163
Size: 573 278 5111
Fragments arranged by size:
5111 573 278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4023 4108 5179 4023
Size: 85 1071 4806
Fragments arranged by size:
4806 1071 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 3177 3281 4023 4108 5179 1391
Size: 399 268 1119 104 742 85 1071 2174
Fragments arranged by size:
2174 1119 1071 742 399 268 104 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2100 3317 844
Size: 1256 1217 3489
Fragments arranged by size:
3489 1256 1217
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2192 3085 4606 4793 1700
Size: 110 382 893 1521 187 2869
Fragments arranged by size:
2869 1521 893 382 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 2905 4022 5034 2905
Size: 1117 1012 3833
Fragments arranged by size:
3833 1117 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2357 2883 3292 3659 4044 4061 5513 5599
Size: 385 526 409 367 385 17 1452 86
Cuts at: 5599 5611 5957 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 526 409 385 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2634 2634
Size: 5962
BtgI C'CryG_G
Cuts at: 513 1065 2004 2487 513
Size: 552 939 483 3988
Fragments arranged by size:
3988 939 552 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 2692 2971 3478 4272 4292 2100
Size: 592 279 507 794 20 3770
Fragments arranged by size:
3770 794 592 507 279 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 2079 3266 2079
Size: 1187 4775
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3409 4298 5927
Size: 54 1425 50 275 18 1579 889 1629
Cuts at: 5927 8
Size: 43
Fragments arranged by size:
1629 1579 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3892 1353
Size: 2539 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3137 4549 4923 5069 387
Size: 1064 138 1548 1412 374 146 1280
Fragments arranged by size:
1548 1412 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2443 2456 2508 4025 4622 5409 5677
Size: 393 811 13 52 1517 597 787 268
Cuts at: 5677 1239
Size: 1524
Fragments arranged by size:
1524 1517 811 787 597 393 268 52
13
EcoO109I rG'GnC_Cy
Cuts at: 2224 2604 2642 2921 2224
Size: 380 38 279 5265
Fragments arranged by size:
5265 380 279 38
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 2539 2571 3123
Size: 32 135 32 770 32 165 32 552
Cuts at: 3123 3155 4603 4635 4677 4709 1373
Size: 32 1448 32 42 32 2626
Fragments arranged by size:
2626 1448 770 552 165 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 5260 1305
Size: 3771 184 2007
Fragments arranged by size:
3771 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3920 4279 4438 518
Size: 648 2754 359 159 2042
Fragments arranged by size:
2754 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3378 4055 5203 5448 1606
Size: 1772 677 1148 245 2120
Fragments arranged by size:
2120 1772 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2487 513
Size: 552 939 483 3988
Fragments arranged by size:
3988 939 552 483
NdeI CA'TA_TG
Cuts at: 387 2248 387
Size: 1861 4101
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NsiI A_TGCA'T
Cuts at: 2320 2320
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 3320 4865 1132
Size: 97 2091 1545 2229
Fragments arranged by size:
2229 2091 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2478 2757 2856 1348
Size: 1130 279 99 4454
Fragments arranged by size:
4454 1130 279 99
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2604 2642 2921 2224
Size: 380 38 279 5265
Fragments arranged by size:
5265 380 279 38
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2798 2830 2798
Size: 32 5930
PstI C_TGCA'G
Cuts at: 830 2641 3314 830
Size: 1811 673 3478
Fragments arranged by size:
3478 1811 673
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2637 3310 4445 5126 5317 826
Size: 219 1592 673 1135 681 191 1471
Fragments arranged by size:
1592 1471 1135 681 673 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 2345 3289 1872
Size: 473 944 4545
Fragments arranged by size:
4545 944 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4066 4967 5229 820
Size: 197 926 2123 901 262 1553
Fragments arranged by size:
2123 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 3401 513
Size: 552 939 483 914 3074
Fragments arranged by size:
3074 939 914 552 483
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2609 2961 3989 4006 4159 4344 4789
Size: 1255 1029 352 1028 17 153 185 445
Cuts at: 4789 325
Size: 1498
Fragments arranged by size:
1498 1255 1029 1028 445 352 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4208 5920 96
Size: 274 80 33 51 1443 2231 1712 138
Fragments arranged by size:
2231 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 2693 2972 1316
Size: 1377 279 4306
Fragments arranged by size:
4306 1377 279
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2485 2485
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 2343 3287 1870
Size: 473 944 4545
Fragments arranged by size:
4545 944 473
XmnI GAAnn'nnTTC
Cuts at: 3117 4091 3117
Size: 974 4988
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI