Restriction map for
pFN21AE0480
(Circular) (Six-base) MAPSORT of: pFN21AE0480.seq Check: 756 from: 1 to: 6130
pFN21AE0480 6130 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3363 3473 1937
Size: 1426 110 4594
Fragments arranged by size:
4594 1426 110
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2338 2460 2841 4193 5577 5845 1632
Size: 706 122 381 1352 1384 268 1917
Fragments arranged by size:
1917 1384 1352 706 381 268 122
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 2268 5029 6006 1203
Size: 1065 2761 977 1327
Fragments arranged by size:
2761 1327 1065 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 2826 4859 2826
Size: 2033 4097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 3873 5445 3873
Size: 1572 4558
ApaLI G'TGCA_C
Cuts at: 2525 4187 5343 2525
Size: 1662 1156 3312
Fragments arranged by size:
3312 1662 1156
ApoI r'AATT_y
Cuts at: 1493 2050 2126 3066 3122 3353 3439 3665 3817
Size: 557 76 940 56 231 86 226 152
Cuts at: 3817 5926 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 940 557 231 226 152 86
76 56
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 3455 1870
Size: 73 1512 4545
Fragments arranged by size:
4545 1512 73
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3451 4906 618
Size: 325 217 627 1664 1455 1842
Fragments arranged by size:
1842 1664 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3449 1391
Size: 2058 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2344 2822 928
Size: 971 445 478 4236
Fragments arranged by size:
4236 971 478 445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BfrBI ATG'CAT
Cuts at: 2762 2762
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2529 4191 5347 1790
Size: 739 1662 1156 2573
Fragments arranged by size:
2573 1662 1156 739
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3861 4819 442
Size: 416 53 83 399 24 2444 958 1753
Fragments arranged by size:
2444 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3036 4790 1239
Size: 1797 1754 2579
Fragments arranged by size:
2579 1797 1754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4255 5120 5418 4255
Size: 865 298 4967
Fragments arranged by size:
4967 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4319 4954 275
Size: 53 83 186 936 2404 382 635 1451
Fragments arranged by size:
2404 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5905
Fragments arranged by size:
5905 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6130
BseYI C'CCAG_C
Cuts at: 5333 5829 5333
Size: 496 5634
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5557
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2529 4191 4276 5347 2529
Size: 1662 85 1071 3312
Fragments arranged by size:
3312 1662 1071 85
BsmI GAATG_Cn'
Cuts at: 2583 2762 3431 3641 3734 2583
Size: 179 669 210 93 4979
Fragments arranged by size:
4979 669 210 179 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2529 3449 4191 4276 5347 1391
Size: 399 739 920 742 85 1071 2174
Fragments arranged by size:
2174 1071 920 742 739 399 85
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 3079 4019 5749 3079
Size: 940 1730 3460
Fragments arranged by size:
3460 1730 940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4774 4961 1700
Size: 110 2964 187 2869
Fragments arranged by size:
2964 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2271 2305 96
Size: 2175 34 3921
Fragments arranged by size:
3921 2175 34
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3290 794
Size: 2496 3634
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 3460 3827 4212 4229 5681 5767 5779 6125
Size: 1488 367 385 17 1452 86 12 346
Cuts at: 6125 1972
Size: 1977
Fragments arranged by size:
1977 1488 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3364 3364
Size: 6130
Bsu36I CC'TnA_GG
Cuts at: 3342 3342
Size: 6130
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 3272 3646 4440 4460 3272
Size: 374 794 20 4942
Fragments arranged by size:
4942 794 374 20
ClaI AT'CG_AT
Cuts at: 1554 2455 1554
Size: 901 5229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3261 3296 529
Size: 35 2697 35 3363
Fragments arranged by size:
3363 2697 35 35
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095
Size: 54 1425 50 275 18 1747 889 1629
Cuts at: 6095 8
Size: 43
Fragments arranged by size:
1747 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2571 4060 1353
Size: 1218 1489 3423
Fragments arranged by size:
3423 1489 1218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2338 2460 2841 3036 4193 4790 5577
Size: 393 706 122 381 195 1157 597 787
Cuts at: 5577 5845 1239
Size: 268 1524
Fragments arranged by size:
1524 1157 787 706 597 393 381 268
195 122
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2240 2240
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 5277 1678
Size: 3599 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2812 2844 2900 2932 3215
Size: 32 135 32 1240 32 56 32 283
Cuts at: 3215 3247 4771 4803 4845 4877 1373
Size: 32 1524 32 42 32 2626
Fragments arranged by size:
2626 1524 1240 283 135 56 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2746 3474 3720 669
Size: 988 281 808 728 246 3079
Fragments arranged by size:
3079 988 808 728 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3247 3278 3729 3247
Size: 31 451 5648
Fragments arranged by size:
5648 451 31
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 5244 5428 1305
Size: 1116 2823 184 2007
Fragments arranged by size:
2823 2007 1116 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4308
Fragments arranged by size:
4308 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2388 4088 4447 4606 518
Size: 648 1222 1700 359 159 2042
Fragments arranged by size:
2042 1700 1222 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3119 3546 4223 5371 5616 1606
Size: 1513 427 677 1148 245 2120
Fragments arranged by size:
2120 1513 1148 677 427 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NsiI A_TGCA'T
Cuts at: 2764 2764
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2272 3288 3488 5033 1132
Size: 97 1043 1016 200 1545 2229
Fragments arranged by size:
2229 1545 1043 1016 200 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 2268 5029 2268
Size: 2761 3369
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 1108
Size: 1035 5095
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2959 2991 2959
Size: 32 6098
PstI C_TGCA'G
Cuts at: 830 3482 830
Size: 2652 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 3119 3119
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 3230 4378 6090 3230
Size: 1148 1712 3270
Fragments arranged by size:
3270 1712 1148
SfcI C'TryA_G
Cuts at: 826 1045 2313 3234 3294 3478 4613 5294 5485
Size: 219 1268 921 60 184 1135 681 191
Cuts at: 5485 826
Size: 1471
Fragments arranged by size:
1471 1268 1135 921 681 219 191 184
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4234 5135 5397 820
Size: 197 926 2291 901 262 1553
Fragments arranged by size:
2291 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 2468 4054 5
Size: 47 2416 1586 2081
Fragments arranged by size:
2416 2081 1586 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 2004 3569 513
Size: 552 939 1565 3074
Fragments arranged by size:
3074 1565 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4157 4174 4327 4512 4957 325
Size: 1255 2577 17 153 185 445 1498
Fragments arranged by size:
2577 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 2305 2975
Size: 274 80 33 51 1443 294 34 670
Cuts at: 2975 3228 4376 6088 96
Size: 253 1148 1712 138
Fragments arranged by size:
1712 1443 1148 670 294 274 253 138
80 51 34 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6090 6122 6090
Size: 32 6098
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3041 3466 3041
Size: 425 5705
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI