Restriction map for
pFN21AE0479
(Circular) (Six-base) MAPSORT of: pFN21AE0479.seq Check: 9012 from: 1 to: 5650
pFN21AE0479 5650 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 3713 5097 5365 1632
Size: 558 1523 1384 268 1917
Fragments arranged by size:
1917 1523 1384 558 268
AfeI AGC'GCT
Cuts at: 2312 2312
Size: 5650
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 2652 4549 5526 1203
Size: 1449 1897 977 1327
Fragments arranged by size:
1897 1449 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 2432 3393 4965 2432
Size: 961 1572 3117
Fragments arranged by size:
3117 1572 961
ApaLI G'TGCA_C
Cuts at: 2041 3707 4863 2041
Size: 1666 1156 2828
Fragments arranged by size:
2828 1666 1156
ApoI r'AATT_y
Cuts at: 1493 2297 2959 3185 3337 5446 1493
Size: 804 662 226 152 2109 1697
Fragments arranged by size:
2109 1697 804 662 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
BamHI G'GATC_C
Cuts at: 2775 2980 3347 2775
Size: 205 367 5078
Fragments arranged by size:
5078 367 205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2817 2971 4426 618
Size: 325 217 627 1030 154 1455 1842
Fragments arranged by size:
1842 1455 1030 627 325 217 154
BanII G_rGCy'C
Cuts at: 1391 2283 2969 1391
Size: 892 686 4072
Fragments arranged by size:
4072 892 686
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4679
BbvCI CC'TCA_GC
Cuts at: 2100 2100
Size: 5650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 2188 2794 1548
Size: 640 606 4404
Fragments arranged by size:
4404 640 606
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2045 3711 4867 1790
Size: 255 1666 1156 2573
Fragments arranged by size:
2573 1666 1156 255
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4310 1239
Size: 3071 2579
Bpu10I CC'TnA_GC
Cuts at: 2100 2100
Size: 5650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2077 3775 4640 4938 2077
Size: 1698 865 298 2789
Fragments arranged by size:
2789 1698 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2499 2756 4313 882
Size: 617 1000 257 1557 2219
Fragments arranged by size:
2219 1557 1000 617 257
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 2427 5644 2427
Size: 3217 2433
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2540 2567 2882 1186
Size: 1213 141 27 315 3954
Fragments arranged by size:
3954 1213 315 141 27
BseYI C'CCAG_C
Cuts at: 4853 5349 4853
Size: 496 5154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 1163
Size: 573 792 4285
Fragments arranged by size:
4285 792 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2045 2283 3711 3796 4867 2045
Size: 238 1428 85 1071 2828
Fragments arranged by size:
2828 1428 1071 238 85
BsmI GAATG_Cn'
Cuts at: 2617 3161 3254 2617
Size: 544 93 5013
Fragments arranged by size:
5013 544 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2283 2969 3711 3796 4867 1391
Size: 399 255 238 686 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 686 399 255 238 85
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 2092 3539 5269 2092
Size: 1447 1730 2473
Fragments arranged by size:
2473 1730 1447
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2019 3005 844
Size: 1175 986 3489
Fragments arranged by size:
3489 1175 986
BsrBI CCG'CTC
Cuts at: 1394 1823 2476 3537 1394
Size: 429 653 1061 3507
Fragments arranged by size:
3507 1061 653 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2511 4145 4319 65
Size: 1183 1263 1634 174 1396
Fragments arranged by size:
1634 1396 1263 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 2086 2655 96
Size: 1990 569 3091
Fragments arranged by size:
3091 1990 569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 2821 2821
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2428 2775 2980 3347 3732 3749 5201 5287
Size: 456 347 205 367 385 17 1452 86
Cuts at: 5287 5299 5645 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 456 385 367 347 346 205
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2550 2550
Size: 5650
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4159
Fragments arranged by size:
4159 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3960 3980 3166
Size: 794 20 4836
Fragments arranged by size:
4836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2899 2934 529
Size: 35 2335 35 3245
Fragments arranged by size:
3245 2335 35 35
DraI TTT'AAA
Cuts at: 2485 2954 2485
Size: 469 5181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2264 4657 809
Size: 507 948 2393 1802
Fragments arranged by size:
2393 1802 948 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2146 3097 3986
Size: 54 1425 50 275 18 316 951 889
Cuts at: 3986 5615 8
Size: 1629 43
Fragments arranged by size:
1629 1425 951 889 316 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2631 2754 3580 1353
Size: 846 432 123 826 3423
Fragments arranged by size:
3423 846 826 432 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2281 2281
Size: 5650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 3713 4310 5097 5365 1239
Size: 393 558 1523 597 787 268 1524
Fragments arranged by size:
1524 1523 787 597 558 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 5650
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 5650
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 2314 4797 1678
Size: 636 2483 2531
Fragments arranged by size:
2531 2483 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2173 2205 4291 4323 4365
Size: 32 135 32 601 32 2086 32 42
Cuts at: 4365 4397 1373
Size: 32 2626
Fragments arranged by size:
2626 2086 601 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 2994 3240 669
Size: 988 281 940 116 246 3079
Fragments arranged by size:
3079 988 940 281 246 116
HindIII A'AGCT_T
Cuts at: 748 2855 748
Size: 2107 3543
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4764 4948 1305
Size: 3459 184 2007
Fragments arranged by size:
3459 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2148 10
Size: 54 1425 343 316 3512
Fragments arranged by size:
3512 1425 343 316 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2124 3608 3967 4126 518
Size: 648 958 1484 359 159 2042
Fragments arranged by size:
2042 1484 958 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2194 3066 3743 4891 5136 1606
Size: 588 872 677 1148 245 2120
Fragments arranged by size:
2120 1148 872 677 588 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4159
Fragments arranged by size:
4159 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2656 3008 4553 1132
Size: 97 1427 352 1545 2229
Fragments arranged by size:
2229 1545 1427 352 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 2652 4549 2652
Size: 1897 3753
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2772 1108
Size: 1664 3986
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 3002 830
Size: 2172 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2194 2194
Size: 5650
SacI G_AGCT'C
Cuts at: 2283 2283
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SexAI A'CCwGG_T
Cuts at: 2820 2820
Size: 5650
SfcI C'TryA_G
Cuts at: 826 1045 2998 4133 4814 5005 826
Size: 219 1953 1135 681 191 1471
Fragments arranged by size:
1953 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2056 3754 4655 4917 820
Size: 197 926 113 1698 901 262 1553
Fragments arranged by size:
1698 1553 926 901 262 197 113
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 2927 1517
Size: 1410 4240
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 2862 3089 513
Size: 552 939 516 342 227 3074
Fragments arranged by size:
3074 939 552 516 342 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 3677 3694 3847 4032 4477 325
Size: 1255 573 1524 17 153 185 445 1498
Fragments arranged by size:
1524 1498 1255 573 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2086 2581 2655
Size: 274 80 33 51 1443 109 495 74
Cuts at: 2655 3896 5608 96
Size: 1241 1712 138
Fragments arranged by size:
1712 1443 1241 495 274 138 109 80
74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2632 2986 2632
Size: 354 5296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2846 2846
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI