Restriction map for
pFN21AE0478
(Circular) (Six-base) MAPSORT of: pFN21AE0478.seq Check: 900 from: 1 to: 5830
pFN21AE0478 5830 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2735 2735
Size: 5830
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3893 5277 5545 1632
Size: 2261 1384 268 1917
Fragments arranged by size:
2261 1917 1384 268
AfeI AGC'GCT
Cuts at: 2688 2809 2688
Size: 121 5709
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 2457 4729 5706 1203
Size: 1254 2272 977 1327
Fragments arranged by size:
2272 1327 1254 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 2720 1166
Size: 1554 4276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2073 1184
Size: 889 4941
AlwNI CAG_nnn'CTG
Cuts at: 2320 3573 5145 2320
Size: 1253 1572 3005
Fragments arranged by size:
3005 1572 1253
ApaI G_GGCC'C
Cuts at: 3118 3118
Size: 5830
ApaLI G'TGCA_C
Cuts at: 2048 3060 3887 5043 2048
Size: 1012 827 1156 2835
Fragments arranged by size:
2835 1156 1012 827
ApoI r'AATT_y
Cuts at: 1493 3139 3365 3517 5626 1493
Size: 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 2372 2960 3117 3155 1870
Size: 73 429 588 157 38 4545
Fragments arranged by size:
4545 588 429 157 73 38
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2327 2393 2875 2938 3151
Size: 325 217 627 540 66 482 63 213
Cuts at: 3151 4606 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 540 482 325 217 213
66 63
BanII G_rGCy'C
Cuts at: 1391 3032 3118 3149 1391
Size: 1641 86 31 4072
Fragments arranged by size:
4072 1641 86 31
BbeI G_GCGC'C
Cuts at: 2331 2942 2331
Size: 611 5219
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BbvCI CC'TCA_GC
Cuts at: 2127 2771 2127
Size: 644 5186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4021 4055 1903
Size: 34 2084 34 3678
Fragments arranged by size:
3678 2084 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BfrBI ATG'CAT
Cuts at: 2820 2820
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2585 2735 4441
Size: 107 122 71 631 605 913 150 1706
Cuts at: 4441 136
Size: 1525
Fragments arranged by size:
1706 1525 913 631 605 150 122 107
71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 2313 3247 2313
Size: 934 4896
Bme1580I G_kGCm'C
Cuts at: 1790 2052 3064 3118 3891 5047 1790
Size: 262 1012 54 773 1156 2573
Fragments arranged by size:
2573 1156 1012 773 262 54
BmgBI CAC'GTC
Cuts at: 1318 2053 1318
Size: 735 5095
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2633 3561 4519
Size: 416 53 83 399 24 1216 928 958
Cuts at: 4519 442
Size: 1753
Fragments arranged by size:
1753 1216 958 928 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2345 1056
Size: 1289 4541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4490 1239
Size: 3251 2579
Bpu10I CC'TnA_GC
Cuts at: 2127 2771 2127
Size: 644 5186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2492 2504 3955 4820 5118 2342
Size: 150 12 1451 865 298 3054
Fragments arranged by size:
3054 1451 865 298 150 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2198 4493 882
Size: 617 699 2295 2219
Fragments arranged by size:
2295 2219 699 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2328 2711 2939
Size: 53 83 186 936 699 96 383 228
Cuts at: 2939 3637 4019 4654 275
Size: 698 382 635 1451
Fragments arranged by size:
1451 936 699 698 635 383 382 228
186 96 83 53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2386 2416 1178
Size: 30 165 30 597 30 356 30 4592
Fragments arranged by size:
4592 597 356 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5830
BseYI C'CCAG_C
Cuts at: 2861 3052 5033 5529 2861
Size: 191 1981 496 3162
Fragments arranged by size:
3162 1981 496 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2771 1163
Size: 573 1035 4222
Fragments arranged by size:
4222 1035 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 2910 3064 3891 3976 5047 2052
Size: 858 154 827 85 1071 2835
Fragments arranged by size:
2835 1071 858 827 154 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
BsmBI CGTCTCn'nnnn_
Cuts at: 2059 2702 2059
Size: 643 5187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2910 3032 3064 3118 3149 3891
Size: 399 262 858 122 32 54 31 742
Cuts at: 3891 3976 5047 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 858 742 399 262 122 85
54 32 31
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 3185 844
Size: 1891 450 3489
Fragments arranged by size:
3489 1891 450
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 4325 4499 65
Size: 1183 1284 1793 174 1396
Fragments arranged by size:
1793 1396 1284 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 2390 4474 4661 1700
Size: 110 376 204 2084 187 2869
Fragments arranged by size:
2869 2084 376 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 2011 2788 1925
Size: 86 777 4967
Fragments arranged by size:
4967 777 86
BssSI C'ACGA_G
Cuts at: 3023 3890 4902 3023
Size: 867 1012 3951
Fragments arranged by size:
3951 1012 867
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5830
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 2178 3160 3527 3912 3929 5381 5467 5479
Size: 206 982 367 385 17 1452 86 12
Cuts at: 5479 5825 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 982 385 367 346 206 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2240 513
Size: 552 939 236 4103
Fragments arranged by size:
4103 939 552 236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 2507 3346 4140 4160 2507
Size: 839 794 20 4177
Fragments arranged by size:
4177 839 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2410 2445 529
Size: 35 1846 35 3914
Fragments arranged by size:
3914 1846 35 35
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2670 3277 4166
Size: 54 1425 50 275 18 840 607 889
Cuts at: 4166 5795 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 840 607 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3760 1353
Size: 2407 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3893 4490 5277 5545 1239
Size: 393 2261 597 787 268 1524
Fragments arranged by size:
2261 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2377 3114 2377
Size: 737 5093
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 2693 4336 2693
Size: 1643 4187
HaeII r_GCGC'y
Cuts at: 1678 2331 2690 2811 2835 2942 4977 1678
Size: 653 359 121 24 107 2035 2531
Fragments arranged by size:
2531 2035 653 359 121 107 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2632 2664 4471
Size: 32 135 32 428 32 600 32 1807
Cuts at: 4471 4503 4545 4577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1807 600 428 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KasI G'GCGC_C
Cuts at: 2327 2938 2327
Size: 611 5219
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2702 2720 3788 4147 4306 518
Size: 648 1536 18 1068 359 159 2042
Fragments arranged by size:
2042 1536 1068 648 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 2044 2103 2242 2407 2919 3246 3923 5071
Size: 438 59 139 165 512 327 677 1148
Cuts at: 5071 5316 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 512 438 327 245 165
139 59
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 5830
NarI GG'CG_CC
Cuts at: 2328 2939 2328
Size: 611 5219
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4339
Fragments arranged by size:
4339 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 2341 1052
Size: 1289 4541
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NsiI A_TGCA'T
Cuts at: 2822 2822
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2824 3188 4733 1132
Size: 97 1595 364 1545 2229
Fragments arranged by size:
2229 1595 1545 364 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PfoI T'CCnGG_A
Cuts at: 2617 2617
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2355 2387 3899 3931 5412 5444 2355
Size: 32 1512 32 1481 32 2741
Fragments arranged by size:
2741 1512 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 2377
Size: 5830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspOMI G'GGCC_C
Cuts at: 3114 3114
Size: 5830
PspXI vC'TCGA_Gb
Cuts at: 2372 2372
Size: 5830
PstI C_TGCA'G
Cuts at: 830 2167 2499 3182 830
Size: 1337 332 683 3478
Fragments arranged by size:
3478 1337 683 332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
PvuII CAG'CTG
Cuts at: 2919 2919
Size: 5830
SacII CC_GC'GG
Cuts at: 2243 2243
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2163 2495 3178 4313 4994 5185 826
Size: 219 1118 332 683 1135 681 191 1471
Fragments arranged by size:
1471 1135 1118 683 681 332 219 191
SfoI GGC'GCC
Cuts at: 2329 2940 2329
Size: 611 5219
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3119 3157 1872
Size: 1247 38 4545
Fragments arranged by size:
4545 1247 38
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2357 2372 2507 2519 3934 4835
Size: 197 926 414 15 135 12 1415 901
Cuts at: 4835 5097 820
Size: 262 1553
Fragments arranged by size:
1553 1415 926 901 414 262 197 135
15 12
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 2517 1517
Size: 1000 4830
StyI C'CwwG_G
Cuts at: 513 1065 2004 3109 3269 513
Size: 552 939 1105 160 3074
Fragments arranged by size:
3074 1105 939 552 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3857 3874 4027 4212 4657 325
Size: 1255 2277 17 153 185 445 1498
Fragments arranged by size:
2277 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4076 5788 96
Size: 274 80 33 51 1443 2099 1712 138
Fragments arranged by size:
2099 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2386 2418 3084 3116 5790 5822 2386
Size: 32 666 32 2674 32 2394
Fragments arranged by size:
2674 2394 666 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2258 3073 1316
Size: 942 815 4073
Fragments arranged by size:
4073 942 815
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 2372 1943
Size: 429 5401
XmaI C'CCGG_G
Cuts at: 1870 3117 3155 1870
Size: 1247 38 4545
Fragments arranged by size:
4545 1247 38
XmnI GAAnn'nnTTC
Cuts at: 2571 2993 3959 2571
Size: 422 966 4442
Fragments arranged by size:
4442 966 422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BplI BsiWI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI PacI PmlI PsrI RsrII SacI SanDI SapI
SexAI SfiI SrfI SwaI XcmI