Restriction map for
pFN21AE0477
(Circular) (Six-base) MAPSORT of: pFN21AE0477.seq Check: 1047 from: 1 to: 5620
pFN21AE0477 5620 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2432 2644 2941 2432
Size: 212 297 5111
Fragments arranged by size:
5111 297 212
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2574 3683 5067 5335 1632
Size: 596 346 1109 1384 268 1917
Fragments arranged by size:
1917 1384 1109 596 346 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 2792 1166
Size: 1626 3994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2810 1184
Size: 1626 3994
AlwNI CAG_nnn'CTG
Cuts at: 2048 3363 4935 2048
Size: 1315 1572 2733
Fragments arranged by size:
2733 1572 1315
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2487 2687 2929 3155 3307 5416 1493
Size: 994 200 242 226 152 2109 1697
Fragments arranged by size:
2109 1697 994 242 226 200 152
AseI AT'TA_AT
Cuts at: 160 1032 2528 4174 160
Size: 872 1496 1646 1606
Fragments arranged by size:
1646 1606 1496 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2432 2644 2941 4396
Size: 325 217 627 249 396 212 297 1455
Cuts at: 4396 618
Size: 1842
Fragments arranged by size:
1842 1455 627 396 325 297 249 217
212
BanII G_rGCy'C
Cuts at: 1391 2939 1391
Size: 1548 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2041 3681 4837 1790
Size: 251 1640 1156 2573
Fragments arranged by size:
2573 1640 1156 251
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4280 1239
Size: 3041 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2811 3745 4610 4908 2811
Size: 934 865 298 3523
Fragments arranged by size:
3523 934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2787 1186
Size: 946 655 4019
Fragments arranged by size:
4019 946 655
BseYI C'CCAG_C
Cuts at: 2041 4823 5319 2041
Size: 2782 496 2342
Fragments arranged by size:
2782 2342 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2094 2816 1163
Size: 573 358 722 3967
Fragments arranged by size:
3967 722 573 358
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3681 3766 4837 3681
Size: 85 1071 4464
Fragments arranged by size:
4464 1071 85
BsmI GAATG_Cn'
Cuts at: 2235 3131 3224 2235
Size: 896 93 4631
Fragments arranged by size:
4631 896 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2939 3681 3766 4837 1391
Size: 399 251 898 742 85 1071 2174
Fragments arranged by size:
2174 1071 898 742 399 251 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 2194 2569 3509 5239 2194
Size: 375 940 1730 2575
Fragments arranged by size:
2575 1730 940 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2747 4115 4289 65
Size: 1183 1499 1368 174 1396
Fragments arranged by size:
1499 1396 1368 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 2010 1925
Size: 85 5535
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 2603 1184
Size: 1419 4201
BstYI r'GATC_y
Cuts at: 1972 2478 2950 3317 3702 3719 5171 5257 5269
Size: 506 472 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 506 472 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5068
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 3067 3956
Size: 54 1425 50 275 18 295 942 889
Cuts at: 3956 5585 8
Size: 1629 43
Fragments arranged by size:
1629 1425 942 889 295 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 2886 3550 1353
Size: 806 727 664 3423
Fragments arranged by size:
3423 806 727 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2574 3683 4280 5067 5335 1239
Size: 393 596 346 1109 597 787 268 1524
Fragments arranged by size:
1524 1109 787 597 596 393 346 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2915 2915
Size: 5620
EcoRI G'AATT_C
Cuts at: 2687 2929 3307 2687
Size: 242 378 5000
Fragments arranged by size:
5000 378 242
EcoRV GAT'ATC
Cuts at: 2802 2802
Size: 5620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2636 2768 2636
Size: 132 5488
FspI TGC'GCA
Cuts at: 2030 4126 2030
Size: 2096 3524
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4261 4293 4335 4367 1373
Size: 32 135 32 2689 32 42 32 2626
Fragments arranged by size:
2689 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2436 2648 2945 2436
Size: 212 297 5111
Fragments arranged by size:
5111 297 212
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2720 4734 4918 1305
Size: 1415 2014 184 2007
Fragments arranged by size:
2014 2007 1415 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3798
Fragments arranged by size:
3798 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 2792 3578 3937 4096 518
Size: 648 1074 552 786 359 159 2042
Fragments arranged by size:
2042 1074 786 648 552 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 3036 3713 4861 5106 1606
Size: 442 988 677 1148 245 2120
Fragments arranged by size:
2120 1148 988 677 442 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5068
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2972 830
Size: 2142 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SapI GCTCTTCn'nnn_
Cuts at: 2886 2886
Size: 5620
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2968 4103 4784 4975 826
Size: 219 1923 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2826 3724 4625 4887 820
Size: 197 926 883 898 901 262 1553
Fragments arranged by size:
1553 926 901 898 883 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2334 2482 2911 3059 513
Size: 552 1269 148 429 148 3074
Fragments arranged by size:
3074 1269 552 429 148 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3647 3664 3817 4002 4447 325
Size: 1255 2067 17 153 185 445 1498
Fragments arranged by size:
2067 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2168 2200 5580 5612 2168
Size: 32 3380 32 2176
Fragments arranged by size:
3380 2176 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2083 1316
Size: 767 4853
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 2399 2466 3749 2399
Size: 67 1283 4270
Fragments arranged by size:
4270 1283 67
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI